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polyclonal goat anti human c5  (Hycult Biotech)


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    Structured Review

    Hycult Biotech polyclonal goat anti human c5
    Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface <t>(polyclonal</t> antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
    Polyclonal Goat Anti Human C5, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal goat anti human c5/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    polyclonal goat anti human c5 - by Bioz Stars, 2025-04
    86/100 stars

    Images

    1) Product Images from "Acinetobacter baumannii Clinical Isolates Resist Complement-Mediated Lysis by Inhibiting the Complement Cascade and Improperly Depositing MAC"

    Article Title: Acinetobacter baumannii Clinical Isolates Resist Complement-Mediated Lysis by Inhibiting the Complement Cascade and Improperly Depositing MAC

    Journal: Journal of Innate Immunity

    doi: 10.1159/000543664

    Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface (polyclonal antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
    Figure Legend Snippet: Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface (polyclonal antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Techniques Used: Bacteria, Titration, Flow Cytometry, Western Blot



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    Hycult Biotech polyclonal goat anti human c5
    Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface <t>(polyclonal</t> antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
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    Alexion Phrama humanized anti c5 monoclonal antibody mab eculizumab
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    Image Search Results


    Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface (polyclonal antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Journal: Journal of Innate Immunity

    Article Title: Acinetobacter baumannii Clinical Isolates Resist Complement-Mediated Lysis by Inhibiting the Complement Cascade and Improperly Depositing MAC

    doi: 10.1159/000543664

    Figure Lengend Snippet: Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface (polyclonal antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Article Snippet: The following antibodies were used for the complement deposition analyses: polyclonal rabbit anti-human C3c (Dako, A0062); polyclonal goat anti-human C5 (Complement Technologies, A220); polyclonal rabbit anti-human C5b (specific for cleavage neoepitope) [ ]; monoclonal mouse anti-human C9 neoantigen (Hycult Biotech, HM2167); polyclonal goat anti-human C9 (Complement Technologies, A226); monoclonal mouse anti-human factor Bb (Quidel, A227); polyclonal goat anti-human factor P (Complement Technologies, A239).

    Techniques: Bacteria, Titration, Flow Cytometry, Western Blot