anti human antibodies  (Cell Signaling Technology Inc)

 
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    Name:
    Human T Cell Co inhibitory and Co stimulatory Receptor IHC Antibody Sampler Kit
    Description:
    The Human T Cell Co inhibitory and Co stimulatory Receptor IHC Antibody Sampler Kit provides an economical means of detecting expression of receptors that modulate T cell activity in formalin fixed paraffin embedded tissue samples
    Catalog Number:
    44689
    Price:
    None
    Category:
    Primary Antibodies
    Source:
    Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein, Gln264 of human OX40 protein, Val142 of human GITR protein, Ala94 of human B7-H3 protein, or near the carboxy terminus of human VISTA protein. Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the extracellular domain of human TIM-3 protein, human 4-1BB/CD137/TNFRSF9 protein, human CD40 ligand protein, or the amino terminus of human LAG3 protein.
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    Structured Review

    Cell Signaling Technology Inc anti human antibodies
    The Human T Cell Co inhibitory and Co stimulatory Receptor IHC Antibody Sampler Kit provides an economical means of detecting expression of receptors that modulate T cell activity in formalin fixed paraffin embedded tissue samples
    https://www.bioz.com/result/anti human antibodies/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human antibodies - by Bioz Stars, 2021-05
    97/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Effects of Interval and Continuous Exercise Training on CD4 Lymphocyte Apoptotic and Autophagic Responses to Hypoxic Stress in Sedentary Men
    Article Snippet: After surface staining, 1 ml of commercial fixation buffer (eBioscience) was added to each sample, and these were incubated at room temperature for 20 min in the dark. .. Following fixation, the cells were washed twice and permeabilized using a commercial permeabilization washing buffer (eBioscience), and then incubated at 4°C for 30 min in the dark with a saturation concentration (10 μg/mL) of monoclonal anti-human mTOR (Cell Signaling), Beclin-1 (Novus Biologicals), Phospho-Bcl-2 (Cell Signaling), or LAMP-2 (eBioscience) monoclonal antibody conjugated with FITC, or anti-IgG (eBioscience) control antibody conjugated with FITC. .. Moreover, the second antibody termed donkey anti-goat IgG (Jackson Immuno Research Laboratories) is conjugated with FITC for binding to the anti-human Atg-1 (Cruz Marker), similarly antibody termed goat anti-rabbit IgG (Jackson Immuno Research Laboratories) is conjugated with FITC for binding with the LC3-II (Cell Signaling) and Atg-12 (Cell Signaling) in dark for 30 min at 4°C.

    Article Title: Hydrogen Sulfide Demonstrates Promising Antitumor Efficacy in Gastric Carcinoma by Targeting MGAT5
    Article Snippet: Apoptotic cells were stained using the Apo-Alert Annexin V kit (BD Biosciences) before analysis. .. Immunofluorescence Staining Cells were fixed with 4% paraformaldehyde at 4°C for 10 min. After washing and pre-blocking, the cells were incubated at 4°C overnight with antibodies against Monoclonal anti-human c-Jun and Monoclonal anti-human MGAT5, respectively, followed by incubation with the FITC-conjugated secondary antibody (1:50; CST) for 1 hour. .. DAPI was used for nuclear staining (10 μg/ml in PBS, Invitrogen, Life Technologies).

    Article Title: Hydrogen Sulfide Demonstrates Promising Antitumor Efficacy in Gastric Carcinoma by Targeting MGAT5
    Article Snippet: Apoptotic cells were stained using the Apo-Alert Annexin V kit (BD Biosciences) before analysis. .. Cells were fixed with 4% paraformaldehyde at 4°C for 10 min. After washing and pre-blocking, the cells were incubated at 4°C overnight with antibodies against Monoclonal anti-human c-Jun and Monoclonal anti-human MGAT5, respectively, followed by incubation with the FITC-conjugated secondary antibody (1:50; CST) for 1 hour. .. DAPI was used for nuclear staining (10 μg/ml in PBS, Invitrogen, Life Technologies).

    Article Title: Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density
    Article Snippet: ImmunohistochemistryImmunohistochemistry was performed automatically on whole tissue sections using a consecutive double staining protocol on a Leica BOND-MAX (Leica Biosystems, Wetzlar, Germany). .. In the first step, antigen retrieval was performed using BOND Epitope Retrieval Solution 2 (Leica Biosystems, Wetzlar, Germany) for 20 min, followed by incubation with anti-human B7-H3 (D9M2L, 1:50, Cell Signaling Technology, Leiden, Netherlands). .. Detection and visualization were done with the Bond Polymer Refine Detection kit (Leica Biosystems, Wetzlar, Germany).

    Concentration Assay:

    Article Title: Effects of Interval and Continuous Exercise Training on CD4 Lymphocyte Apoptotic and Autophagic Responses to Hypoxic Stress in Sedentary Men
    Article Snippet: After surface staining, 1 ml of commercial fixation buffer (eBioscience) was added to each sample, and these were incubated at room temperature for 20 min in the dark. .. Following fixation, the cells were washed twice and permeabilized using a commercial permeabilization washing buffer (eBioscience), and then incubated at 4°C for 30 min in the dark with a saturation concentration (10 μg/mL) of monoclonal anti-human mTOR (Cell Signaling), Beclin-1 (Novus Biologicals), Phospho-Bcl-2 (Cell Signaling), or LAMP-2 (eBioscience) monoclonal antibody conjugated with FITC, or anti-IgG (eBioscience) control antibody conjugated with FITC. .. Moreover, the second antibody termed donkey anti-goat IgG (Jackson Immuno Research Laboratories) is conjugated with FITC for binding to the anti-human Atg-1 (Cruz Marker), similarly antibody termed goat anti-rabbit IgG (Jackson Immuno Research Laboratories) is conjugated with FITC for binding with the LC3-II (Cell Signaling) and Atg-12 (Cell Signaling) in dark for 30 min at 4°C.

    SDS Page:

    Article Title: MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells
    Article Snippet: .. Then, the lysates were heated for 5 min at 98 °C and analyzed by SDS-PAGE, followed by western blotting using the following antibodies: anti-human RIP1 antibody (1 : 1000, monoclonal mouse IgG clone no. 334640; R & D Systems Inc., Minneapolis, MN, USA), anti-human RIP3 antibody (1 : 1000, monoclonal rabbit IgG no. 13526; Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-p53 antibody (7F5) (1 : 1000, monoclonal rabbit IgG no. 2527; Cell Signaling Technology Japan), anti-phosphor-histone H2A.X antibody (1 : 1000, monoclonal mouse IgG clone JBW301; Millipore), anti-GAPDH antibody (1 : 2000, monoclonal mouse IgG clone 6C5 (Millipore) or 1 : 2000, monoclonal rabbit IgG clone 14C10 (Cell Signaling Technology Japan)), HRP-conjugated anti-mouse antibody (1 : 2000, monoclonal goat IgG no. C2011; Santa Cruz Biotechnology, Dallas, TX, USA) and HRP-conjugated anti-rabbit antibody (1 : 2000, monoclonal goat IgG no. 7074; Cell Signaling Technology Japan). .. Detection of the mitochondrial membrane potential The mitochondrial membrane potential was assessed by staining with TMRM (Cosmo Bio Co., Tokyo, Japan).

    Western Blot:

    Article Title: MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells
    Article Snippet: .. Then, the lysates were heated for 5 min at 98 °C and analyzed by SDS-PAGE, followed by western blotting using the following antibodies: anti-human RIP1 antibody (1 : 1000, monoclonal mouse IgG clone no. 334640; R & D Systems Inc., Minneapolis, MN, USA), anti-human RIP3 antibody (1 : 1000, monoclonal rabbit IgG no. 13526; Cell Signaling Technology Japan, K.K., Tokyo, Japan), anti-p53 antibody (7F5) (1 : 1000, monoclonal rabbit IgG no. 2527; Cell Signaling Technology Japan), anti-phosphor-histone H2A.X antibody (1 : 1000, monoclonal mouse IgG clone JBW301; Millipore), anti-GAPDH antibody (1 : 2000, monoclonal mouse IgG clone 6C5 (Millipore) or 1 : 2000, monoclonal rabbit IgG clone 14C10 (Cell Signaling Technology Japan)), HRP-conjugated anti-mouse antibody (1 : 2000, monoclonal goat IgG no. C2011; Santa Cruz Biotechnology, Dallas, TX, USA) and HRP-conjugated anti-rabbit antibody (1 : 2000, monoclonal goat IgG no. 7074; Cell Signaling Technology Japan). .. Detection of the mitochondrial membrane potential The mitochondrial membrane potential was assessed by staining with TMRM (Cosmo Bio Co., Tokyo, Japan).

    Immunofluorescence:

    Article Title: Hydrogen Sulfide Demonstrates Promising Antitumor Efficacy in Gastric Carcinoma by Targeting MGAT5
    Article Snippet: Apoptotic cells were stained using the Apo-Alert Annexin V kit (BD Biosciences) before analysis. .. Immunofluorescence Staining Cells were fixed with 4% paraformaldehyde at 4°C for 10 min. After washing and pre-blocking, the cells were incubated at 4°C overnight with antibodies against Monoclonal anti-human c-Jun and Monoclonal anti-human MGAT5, respectively, followed by incubation with the FITC-conjugated secondary antibody (1:50; CST) for 1 hour. .. DAPI was used for nuclear staining (10 μg/ml in PBS, Invitrogen, Life Technologies).

    Staining:

    Article Title: Hydrogen Sulfide Demonstrates Promising Antitumor Efficacy in Gastric Carcinoma by Targeting MGAT5
    Article Snippet: Apoptotic cells were stained using the Apo-Alert Annexin V kit (BD Biosciences) before analysis. .. Immunofluorescence Staining Cells were fixed with 4% paraformaldehyde at 4°C for 10 min. After washing and pre-blocking, the cells were incubated at 4°C overnight with antibodies against Monoclonal anti-human c-Jun and Monoclonal anti-human MGAT5, respectively, followed by incubation with the FITC-conjugated secondary antibody (1:50; CST) for 1 hour. .. DAPI was used for nuclear staining (10 μg/ml in PBS, Invitrogen, Life Technologies).

    other:

    Article Title: B7-H3 regulates migration and invasion in salivary gland adenoid cystic carcinoma via the JAK2/STAT3 signaling pathway
    Article Snippet: Primary antibody against human B7-H3, E-cadherin, N-cadherin, Vimentin, Slug and p-STAT3 were purchased from Cell Signaling Technology (Danvers, MA).

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  • 99
    Cell Signaling Technology Inc monoclonal rabbit anti human antibody against β actin
    Expression of Six1 in prostate cancer tissues and normal prostate tissues detected by western blotting and immunochemistry. a Relative mRNA expression of Six1 normalized to <t>β-actin</t> was calculated ( n = 8), ★ indicates P
    Monoclonal Rabbit Anti Human Antibody Against β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rabbit anti human antibody against β actin/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal rabbit anti human antibody against β actin - by Bioz Stars, 2021-05
    99/100 stars
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    94
    Cell Signaling Technology Inc anti human cd133
    Colorectal cancer samples with membranous positivity and corresponding negative staining for <t>CD133</t> ( A and B ), CD166 ( C and D ), CD44s ( E and F ), EpCAM ( G and H ) and cytoplasmic positivity and negativity for ALDH1 ( I and J ).
    Anti Human Cd133, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cd133/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human cd133 - by Bioz Stars, 2021-05
    94/100 stars
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    99
    Cell Signaling Technology Inc anti caspase 9
    BYD inhibits apoptosis in CM-induced H9C2 cells.  (A)  Flow diagram of CM-induced H9C2 cell injury.  (B)  BYD showed a significant protective effect on CM-induced H9C2 cell injury.  (C)  BYD could increase the release of T-SOD from H9C2 injuried by CM.  (D)  Hoechst 33258 staining results showed that BYD attenuated apoptosis in CM-induced H9C2 cells.  (E)  BYD could up-regulate the expression of p-CRYAB  in vitro .  (F)  Different doses of BYD treatments down-regulated the increased expression of Bax, cleaved-caspase 9 and cleaved-caspase 3 compared with that in the model group. The same GAPDH band was selected due to reusing the membrane by stripping solution. All the data were presented as the means ± SEM from independent experiments performed in triplicate.  ∗ P
    Anti Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 9/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 9 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Expression of Six1 in prostate cancer tissues and normal prostate tissues detected by western blotting and immunochemistry. a Relative mRNA expression of Six1 normalized to β-actin was calculated ( n = 8), ★ indicates P

    Journal: Cancer Cell International

    Article Title: Increased expression of Six1 correlates with progression and prognosis of prostate cancer

    doi: 10.1186/s12935-015-0215-z

    Figure Lengend Snippet: Expression of Six1 in prostate cancer tissues and normal prostate tissues detected by western blotting and immunochemistry. a Relative mRNA expression of Six1 normalized to β-actin was calculated ( n = 8), ★ indicates P

    Article Snippet: After the blots were washed with 1 × TBST buffer (10 mM Tris-HCl [pH 7.6], 150 mM NaCl, and 0.05 % Tween-20), the membranes were blocked overnight with 5 % skim milk and incubated with the appropriate primary antibody at room temperature for 2 h. Polyclonal rabbit anti-human antibody against Six1 (Atlas antibody, Sweden, 1:500), and monoclonal rabbit anti-human antibody against β-actin (Cell Signaling Technology, Danvers, MA, USA, 1:3000) were used for detecting the protein level of Six1 and β-actin in each sample.

    Techniques: Expressing, Western Blot

    Colorectal cancer samples with membranous positivity and corresponding negative staining for CD133 ( A and B ), CD166 ( C and D ), CD44s ( E and F ), EpCAM ( G and H ) and cytoplasmic positivity and negativity for ALDH1 ( I and J ).

    Journal: British Journal of Cancer

    Article Title: Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM, and ALDH1 in colorectal cancer

    doi: 10.1038/sj.bjc.6605762

    Figure Lengend Snippet: Colorectal cancer samples with membranous positivity and corresponding negative staining for CD133 ( A and B ), CD166 ( C and D ), CD44s ( E and F ), EpCAM ( G and H ) and cytoplasmic positivity and negativity for ALDH1 ( I and J ).

    Article Snippet: The following primary antibodies were used: anti-human CD133 (clone C24B9; 1:100; Cell Signaling, Allschwil, Swizerland), anti-human CD166 (clone M0G/07; 1:200; Novocastra, Newcastle, UK), anti-human CD44s (clone DF1485; 1:50; Dako, Glostrup, Denmark), anti-human EpCAM (clone VU-1D9; 1:200; Novocastra), and anti-human ALDH1 isoform α1(polyclonal; 1:500; AbCam, Cambridge, UK).

    Techniques: Negative Staining

    BYD inhibits apoptosis in CM-induced H9C2 cells.  (A)  Flow diagram of CM-induced H9C2 cell injury.  (B)  BYD showed a significant protective effect on CM-induced H9C2 cell injury.  (C)  BYD could increase the release of T-SOD from H9C2 injuried by CM.  (D)  Hoechst 33258 staining results showed that BYD attenuated apoptosis in CM-induced H9C2 cells.  (E)  BYD could up-regulate the expression of p-CRYAB  in vitro .  (F)  Different doses of BYD treatments down-regulated the increased expression of Bax, cleaved-caspase 9 and cleaved-caspase 3 compared with that in the model group. The same GAPDH band was selected due to reusing the membrane by stripping solution. All the data were presented as the means ± SEM from independent experiments performed in triplicate.  ∗ P

    Journal: Frontiers in Physiology

    Article Title: BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway

    doi: 10.3389/fphys.2018.00505

    Figure Lengend Snippet: BYD inhibits apoptosis in CM-induced H9C2 cells. (A) Flow diagram of CM-induced H9C2 cell injury. (B) BYD showed a significant protective effect on CM-induced H9C2 cell injury. (C) BYD could increase the release of T-SOD from H9C2 injuried by CM. (D) Hoechst 33258 staining results showed that BYD attenuated apoptosis in CM-induced H9C2 cells. (E) BYD could up-regulate the expression of p-CRYAB in vitro . (F) Different doses of BYD treatments down-regulated the increased expression of Bax, cleaved-caspase 9 and cleaved-caspase 3 compared with that in the model group. The same GAPDH band was selected due to reusing the membrane by stripping solution. All the data were presented as the means ± SEM from independent experiments performed in triplicate. ∗ P

    Article Snippet: The following antibodies were used: anti-CRYAB phosphor-S59 (ab5577; Abcam, United States), anti-CRYAB (ab13497; Abcam, United States), anti-phospho MAPK-activated protein kinase 2 (MAPKAPK2; 3041; Cell Signaling Technology, Germany), anti-MAPKAPK2 (3042; Cell Signaling Technology, Germany), anti-phospho MKK 3/MKK6 (9236; Cell Signaling Technology, Germany), anti-MKK6 (8550; Cell Signaling Technology, Germany), anti-phospho P38 MAPK (4511; Cell Signaling Technology, Germany), anti-P38 MAPK (8690; Cell Signaling Technology, Germany), anti-Caspase 3 (9665; Cell Signaling Technology, Germany), anti-Caspase 9 (9580; Cell Signaling Technology, Germany), anti-Bax (ab32503; Abcam, United States), anti-Bcl-2 (ab7973; Abcam, United States), anti-GAPDH (ab8245; Abcam, United States), anti-rabbit IgG H & L (HRP; ab16284; Abcam, United States), anti-mouse IgG H & L (HRP; ab97250; Abcam, United States).

    Techniques: Flow Cytometry, Staining, Expressing, In Vitro, Stripping Membranes

    BYD inhibited apoptosis and increased CRYAB in HF post-AMI rats.  (A)  TUNEL results showed that BYD attenuated the apoptosis of cardiomyocytes.  (B)  Western blot analysis showed that BYD decreased the expression of Bax, cleaved-caspase 9 and cleaved-caspase 3 and increased the expressions of Bcl-2 in cardiac tissue compared with that in the model group.  (C)  Immunohistochemistry results showed that BYD up-regulated the expression of p-CRYAB in cardiac tissue.  (D)  Western blot analysis results showed that BYD up-regulated the expression of p-CRYAB in cardiac tissue compared with that in the model group. The same GAPDH band was selected due to reusing the membrane by stripping solution. All the data were presented as the means ± SEM from independent experiments performed in triplicate.  ∗ P

    Journal: Frontiers in Physiology

    Article Title: BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway

    doi: 10.3389/fphys.2018.00505

    Figure Lengend Snippet: BYD inhibited apoptosis and increased CRYAB in HF post-AMI rats. (A) TUNEL results showed that BYD attenuated the apoptosis of cardiomyocytes. (B) Western blot analysis showed that BYD decreased the expression of Bax, cleaved-caspase 9 and cleaved-caspase 3 and increased the expressions of Bcl-2 in cardiac tissue compared with that in the model group. (C) Immunohistochemistry results showed that BYD up-regulated the expression of p-CRYAB in cardiac tissue. (D) Western blot analysis results showed that BYD up-regulated the expression of p-CRYAB in cardiac tissue compared with that in the model group. The same GAPDH band was selected due to reusing the membrane by stripping solution. All the data were presented as the means ± SEM from independent experiments performed in triplicate. ∗ P

    Article Snippet: The following antibodies were used: anti-CRYAB phosphor-S59 (ab5577; Abcam, United States), anti-CRYAB (ab13497; Abcam, United States), anti-phospho MAPK-activated protein kinase 2 (MAPKAPK2; 3041; Cell Signaling Technology, Germany), anti-MAPKAPK2 (3042; Cell Signaling Technology, Germany), anti-phospho MKK 3/MKK6 (9236; Cell Signaling Technology, Germany), anti-MKK6 (8550; Cell Signaling Technology, Germany), anti-phospho P38 MAPK (4511; Cell Signaling Technology, Germany), anti-P38 MAPK (8690; Cell Signaling Technology, Germany), anti-Caspase 3 (9665; Cell Signaling Technology, Germany), anti-Caspase 9 (9580; Cell Signaling Technology, Germany), anti-Bax (ab32503; Abcam, United States), anti-Bcl-2 (ab7973; Abcam, United States), anti-GAPDH (ab8245; Abcam, United States), anti-rabbit IgG H & L (HRP; ab16284; Abcam, United States), anti-mouse IgG H & L (HRP; ab97250; Abcam, United States).

    Techniques: TUNEL Assay, Western Blot, Expressing, Immunohistochemistry, Stripping Membranes

    Methazolamide inhibits the release of mitochondrial apoptogenic factors and forestalls the activation of caspase-9 and -3. A , Cell death is induced in mutant-htt striatal cells by shifting them to the nonpermissive temperature of 37°C in SDM. Test cell cultures contain 100 μ M methazolamide, whereas controls are devoid of these drugs. After 5 h, the cells are stained with Mitotracker and then fixed and stained with antibodies to cytochrome c . Mitochondrial cytochrome c is demonstrated as having a punctate pattern that colocalizes with Mitotracker. Cells in which cytochrome c is released demonstrate a more diffuse and decreased intensity of cytochrome c staining (arrows). Methazolamide retains intensity and punctate characteristics of the cytochrome c staining (Scale bar, 5 μ m). B , Mutant-htt striatal cells are treated with 100 μ M methazolamide (or processed in the absence of methazolamide) for 18 h. Subsequently, they were extracted; either cytosolic components or whole cell lysates were obtained. The samples, each of which contains 50 μ g of protein, were analyzed by Western blot using antibodies either to cytochrome c , Smac with cytosolic components, or to caspase-9, or caspase-3 with whole cell lysates. β -Actin was used as a loading control. This blot is representative of three independent experiments. Densitometry was performed to quantify the intensity of the bands from the three independent experiments (* p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Inhibitors of Cytochrome c Release with Therapeutic Potential for Huntington's Disease

    doi: 10.1523/JNEUROSCI.1867-08.2008

    Figure Lengend Snippet: Methazolamide inhibits the release of mitochondrial apoptogenic factors and forestalls the activation of caspase-9 and -3. A , Cell death is induced in mutant-htt striatal cells by shifting them to the nonpermissive temperature of 37°C in SDM. Test cell cultures contain 100 μ M methazolamide, whereas controls are devoid of these drugs. After 5 h, the cells are stained with Mitotracker and then fixed and stained with antibodies to cytochrome c . Mitochondrial cytochrome c is demonstrated as having a punctate pattern that colocalizes with Mitotracker. Cells in which cytochrome c is released demonstrate a more diffuse and decreased intensity of cytochrome c staining (arrows). Methazolamide retains intensity and punctate characteristics of the cytochrome c staining (Scale bar, 5 μ m). B , Mutant-htt striatal cells are treated with 100 μ M methazolamide (or processed in the absence of methazolamide) for 18 h. Subsequently, they were extracted; either cytosolic components or whole cell lysates were obtained. The samples, each of which contains 50 μ g of protein, were analyzed by Western blot using antibodies either to cytochrome c , Smac with cytosolic components, or to caspase-9, or caspase-3 with whole cell lysates. β -Actin was used as a loading control. This blot is representative of three independent experiments. Densitometry was performed to quantify the intensity of the bands from the three independent experiments (* p

    Article Snippet: Antibody to caspase-3 were purchased from Cell Signaling Technology, antibody to caspase-9 from Cell Signaling Technology, antibody to β -actin from Sigma-Aldrich, and secondary antibodies and ECL reagents from GE Healthcare.

    Techniques: Activation Assay, Mutagenesis, Staining, Western Blot