anti heparanase  (Thermo Fisher)


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  • 90
    Name:
    anti Heparanase Polyclonal
    Description:

    Catalog Number:
    PA5-11688
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    Structured Review

    Thermo Fisher anti heparanase
    <t>Heparanase,</t> 8C and T5 induction enhances tumor xenograft development. A. Tumor volume. Heparanase-, 8C-, and T5-infected CAG myeloma cells were injected subcutaneously (1×10 6 /0.1 ml) and tumor development in mice supplemented (red) or not supplemented

    https://www.bioz.com/result/anti heparanase/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti heparanase - by Bioz Stars, 2021-07
    90/100 stars

    Images

    1) Product Images from "Heparanase enhances myeloma progression via CXCL10 down regulation"

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation

    Journal: Leukemia

    doi: 10.1038/leu.2014.121

    Heparanase, 8C and T5 induction enhances tumor xenograft development. A. Tumor volume. Heparanase-, 8C-, and T5-infected CAG myeloma cells were injected subcutaneously (1×10 6 /0.1 ml) and tumor development in mice supplemented (red) or not supplemented
    Figure Legend Snippet: Heparanase, 8C and T5 induction enhances tumor xenograft development. A. Tumor volume. Heparanase-, 8C-, and T5-infected CAG myeloma cells were injected subcutaneously (1×10 6 /0.1 ml) and tumor development in mice supplemented (red) or not supplemented

    Techniques Used: Infection, Injection, Mouse Assay

    Validation of gene array results. Total RNA was extracted from CAG cell clone 13 carrying Tet-inducible heparanase before (0) and 8, 16, and 24 hours after the addition of Dox and subjected to gene array analysis as described under ‘Materials
    Figure Legend Snippet: Validation of gene array results. Total RNA was extracted from CAG cell clone 13 carrying Tet-inducible heparanase before (0) and 8, 16, and 24 hours after the addition of Dox and subjected to gene array analysis as described under ‘Materials

    Techniques Used:

    Heparanase down-regulates CXCL10 expression. A . Total RNA was extracted from CAG-heparanase Tet-on clone 1 cells before (0) and 8, 16, and 24 hours after the addition of Dox, and CXCL10 expression was quantified by real time PCR. B . CAG-heparanase clone
    Figure Legend Snippet: Heparanase down-regulates CXCL10 expression. A . Total RNA was extracted from CAG-heparanase Tet-on clone 1 cells before (0) and 8, 16, and 24 hours after the addition of Dox, and CXCL10 expression was quantified by real time PCR. B . CAG-heparanase clone

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    CXCL10 attenuates tumor xenograft development. CAG-heparanase cells were infected with CXCL10 or control empty vector (Vo) and inoculated (1×10 6 ) subcutaneously into SCID mice (n=9-10) and tumor development was calculated from external caliper
    Figure Legend Snippet: CXCL10 attenuates tumor xenograft development. CAG-heparanase cells were infected with CXCL10 or control empty vector (Vo) and inoculated (1×10 6 ) subcutaneously into SCID mice (n=9-10) and tumor development was calculated from external caliper

    Techniques Used: Infection, Plasmid Preparation, Mouse Assay

    Inducible expression of heparanase, 8C and T5. A . CAG myeloma cells were infected with Tet-inducible heparanase, 8C, or T5 gene constructs. Cells were left untreated (-) or incubated with Dox (1μg/ml; +) for 24 h. Cell lysates were then prepared
    Figure Legend Snippet: Inducible expression of heparanase, 8C and T5. A . CAG myeloma cells were infected with Tet-inducible heparanase, 8C, or T5 gene constructs. Cells were left untreated (-) or incubated with Dox (1μg/ml; +) for 24 h. Cell lysates were then prepared

    Techniques Used: Expressing, Infection, Construct, Incubation

    2) Product Images from "Heparanase induces VEGF C and facilitates tumor lymphangiogenesis"

    Article Title: Heparanase induces VEGF C and facilitates tumor lymphangiogenesis

    Journal: International journal of cancer. Journal international du cancer

    doi: 10.1002/ijc.23898

    Heparanase staining correlates with VEGF C expression
    Figure Legend Snippet: Heparanase staining correlates with VEGF C expression

    Techniques Used: Staining, Expressing

    Heparanase expression correlates with LVD
    Figure Legend Snippet: Heparanase expression correlates with LVD

    Techniques Used: Expressing

    Heparanase induces VEGF C expression. A . Tumor-derived cell lines were stably transfected with control empty vector (Vo, ◆) or heparanase (Hepa, ■) plasmid and total cell lysates were subjected to heparanase activity assay (upper panel)
    Figure Legend Snippet: Heparanase induces VEGF C expression. A . Tumor-derived cell lines were stably transfected with control empty vector (Vo, ◆) or heparanase (Hepa, ■) plasmid and total cell lysates were subjected to heparanase activity assay (upper panel)

    Techniques Used: Expressing, Derivative Assay, Stable Transfection, Transfection, Plasmid Preparation, Activity Assay

    Heparanase facilitates tumor xenograft lymphangiogenesis. Control (Vo) and heparanase (Hepa) transfected MDA-435 cells (5×10 6 ) were inoculated at the flank of Balb C/nude mice. Tumor xenografts were harvested after 6 weeks and 5 micron sections
    Figure Legend Snippet: Heparanase facilitates tumor xenograft lymphangiogenesis. Control (Vo) and heparanase (Hepa) transfected MDA-435 cells (5×10 6 ) were inoculated at the flank of Balb C/nude mice. Tumor xenografts were harvested after 6 weeks and 5 micron sections

    Techniques Used: Transfection, Multiple Displacement Amplification, Mouse Assay

    Related Articles

    SDS Page:

    Article Title: Heparanase induces VEGF C and facilitates tumor lymphangiogenesis
    Article Snippet: Total cellular protein concentration was determined by the BCA assay, according to the manufacturer's instructions (Pierce, Rockford, IL). .. Thirty μg of cellular protein were resolved on SDS polyacrylamid gel (SDS-PAGE) and immunoblotting was performed applying anti-heparanase, anti-VEGF C (Zymed, South San Francisco, CA) and anti-actin (Sigma, St. Louis, MI) antibodies, as described , . .. Total RNA was extracted with TRIzol (Gibco BRL Life Technologies, Rockville, MD) and RNA (1 μg) was amplified using one step PCR amplification kit, according to the manufacturer's instructions (ABgene, Epsom, UK) essentially as described .

    shRNA:

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation
    Article Snippet: Human CXCL10 cDNA was purchased from OriGene (Rockville, MD) and sub-cloned into NSPI-CMV-MCS-myc-HIS viral plasmid . .. Anti-heparanase and anti-CXCL10 shRNAs (GIPZ lentiviral shRNA) were purchased from Thermo Scientific (Waltham, MA). .. Mouse IgG1 Fc (Hinge CH2-CH3) constant region was cloned from RNA extracted from mouse splenocytes that were cultured for 96 h in the presence of LPS and mouse IL-4.

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  • 90
    Thermo Fisher anti heparanase
    <t>Heparanase,</t> 8C and T5 induction enhances tumor xenograft development. A. Tumor volume. Heparanase-, 8C-, and T5-infected CAG myeloma cells were injected subcutaneously (1×10 6 /0.1 ml) and tumor development in mice supplemented (red) or not supplemented
    Anti Heparanase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti heparanase/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti heparanase - by Bioz Stars, 2021-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Heparanase, 8C and T5 induction enhances tumor xenograft development. A. Tumor volume. Heparanase-, 8C-, and T5-infected CAG myeloma cells were injected subcutaneously (1×10 6 /0.1 ml) and tumor development in mice supplemented (red) or not supplemented

    Journal: Leukemia

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation

    doi: 10.1038/leu.2014.121

    Figure Lengend Snippet: Heparanase, 8C and T5 induction enhances tumor xenograft development. A. Tumor volume. Heparanase-, 8C-, and T5-infected CAG myeloma cells were injected subcutaneously (1×10 6 /0.1 ml) and tumor development in mice supplemented (red) or not supplemented

    Article Snippet: Anti-heparanase and anti-CXCL10 shRNAs (GIPZ lentiviral shRNA) were purchased from Thermo Scientific (Waltham, MA).

    Techniques: Infection, Injection, Mouse Assay

    Validation of gene array results. Total RNA was extracted from CAG cell clone 13 carrying Tet-inducible heparanase before (0) and 8, 16, and 24 hours after the addition of Dox and subjected to gene array analysis as described under ‘Materials

    Journal: Leukemia

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation

    doi: 10.1038/leu.2014.121

    Figure Lengend Snippet: Validation of gene array results. Total RNA was extracted from CAG cell clone 13 carrying Tet-inducible heparanase before (0) and 8, 16, and 24 hours after the addition of Dox and subjected to gene array analysis as described under ‘Materials

    Article Snippet: Anti-heparanase and anti-CXCL10 shRNAs (GIPZ lentiviral shRNA) were purchased from Thermo Scientific (Waltham, MA).

    Techniques:

    Heparanase down-regulates CXCL10 expression. A . Total RNA was extracted from CAG-heparanase Tet-on clone 1 cells before (0) and 8, 16, and 24 hours after the addition of Dox, and CXCL10 expression was quantified by real time PCR. B . CAG-heparanase clone

    Journal: Leukemia

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation

    doi: 10.1038/leu.2014.121

    Figure Lengend Snippet: Heparanase down-regulates CXCL10 expression. A . Total RNA was extracted from CAG-heparanase Tet-on clone 1 cells before (0) and 8, 16, and 24 hours after the addition of Dox, and CXCL10 expression was quantified by real time PCR. B . CAG-heparanase clone

    Article Snippet: Anti-heparanase and anti-CXCL10 shRNAs (GIPZ lentiviral shRNA) were purchased from Thermo Scientific (Waltham, MA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    CXCL10 attenuates tumor xenograft development. CAG-heparanase cells were infected with CXCL10 or control empty vector (Vo) and inoculated (1×10 6 ) subcutaneously into SCID mice (n=9-10) and tumor development was calculated from external caliper

    Journal: Leukemia

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation

    doi: 10.1038/leu.2014.121

    Figure Lengend Snippet: CXCL10 attenuates tumor xenograft development. CAG-heparanase cells were infected with CXCL10 or control empty vector (Vo) and inoculated (1×10 6 ) subcutaneously into SCID mice (n=9-10) and tumor development was calculated from external caliper

    Article Snippet: Anti-heparanase and anti-CXCL10 shRNAs (GIPZ lentiviral shRNA) were purchased from Thermo Scientific (Waltham, MA).

    Techniques: Infection, Plasmid Preparation, Mouse Assay

    Inducible expression of heparanase, 8C and T5. A . CAG myeloma cells were infected with Tet-inducible heparanase, 8C, or T5 gene constructs. Cells were left untreated (-) or incubated with Dox (1μg/ml; +) for 24 h. Cell lysates were then prepared

    Journal: Leukemia

    Article Title: Heparanase enhances myeloma progression via CXCL10 down regulation

    doi: 10.1038/leu.2014.121

    Figure Lengend Snippet: Inducible expression of heparanase, 8C and T5. A . CAG myeloma cells were infected with Tet-inducible heparanase, 8C, or T5 gene constructs. Cells were left untreated (-) or incubated with Dox (1μg/ml; +) for 24 h. Cell lysates were then prepared

    Article Snippet: Anti-heparanase and anti-CXCL10 shRNAs (GIPZ lentiviral shRNA) were purchased from Thermo Scientific (Waltham, MA).

    Techniques: Expressing, Infection, Construct, Incubation

    Heparanase staining correlates with VEGF C expression

    Journal: International journal of cancer. Journal international du cancer

    Article Title: Heparanase induces VEGF C and facilitates tumor lymphangiogenesis

    doi: 10.1002/ijc.23898

    Figure Lengend Snippet: Heparanase staining correlates with VEGF C expression

    Article Snippet: Thirty μg of cellular protein were resolved on SDS polyacrylamid gel (SDS-PAGE) and immunoblotting was performed applying anti-heparanase, anti-VEGF C (Zymed, South San Francisco, CA) and anti-actin (Sigma, St. Louis, MI) antibodies, as described , .

    Techniques: Staining, Expressing

    Heparanase expression correlates with LVD

    Journal: International journal of cancer. Journal international du cancer

    Article Title: Heparanase induces VEGF C and facilitates tumor lymphangiogenesis

    doi: 10.1002/ijc.23898

    Figure Lengend Snippet: Heparanase expression correlates with LVD

    Article Snippet: Thirty μg of cellular protein were resolved on SDS polyacrylamid gel (SDS-PAGE) and immunoblotting was performed applying anti-heparanase, anti-VEGF C (Zymed, South San Francisco, CA) and anti-actin (Sigma, St. Louis, MI) antibodies, as described , .

    Techniques: Expressing

    Heparanase induces VEGF C expression. A . Tumor-derived cell lines were stably transfected with control empty vector (Vo, ◆) or heparanase (Hepa, ■) plasmid and total cell lysates were subjected to heparanase activity assay (upper panel)

    Journal: International journal of cancer. Journal international du cancer

    Article Title: Heparanase induces VEGF C and facilitates tumor lymphangiogenesis

    doi: 10.1002/ijc.23898

    Figure Lengend Snippet: Heparanase induces VEGF C expression. A . Tumor-derived cell lines were stably transfected with control empty vector (Vo, ◆) or heparanase (Hepa, ■) plasmid and total cell lysates were subjected to heparanase activity assay (upper panel)

    Article Snippet: Thirty μg of cellular protein were resolved on SDS polyacrylamid gel (SDS-PAGE) and immunoblotting was performed applying anti-heparanase, anti-VEGF C (Zymed, South San Francisco, CA) and anti-actin (Sigma, St. Louis, MI) antibodies, as described , .

    Techniques: Expressing, Derivative Assay, Stable Transfection, Transfection, Plasmid Preparation, Activity Assay

    Heparanase facilitates tumor xenograft lymphangiogenesis. Control (Vo) and heparanase (Hepa) transfected MDA-435 cells (5×10 6 ) were inoculated at the flank of Balb C/nude mice. Tumor xenografts were harvested after 6 weeks and 5 micron sections

    Journal: International journal of cancer. Journal international du cancer

    Article Title: Heparanase induces VEGF C and facilitates tumor lymphangiogenesis

    doi: 10.1002/ijc.23898

    Figure Lengend Snippet: Heparanase facilitates tumor xenograft lymphangiogenesis. Control (Vo) and heparanase (Hepa) transfected MDA-435 cells (5×10 6 ) were inoculated at the flank of Balb C/nude mice. Tumor xenografts were harvested after 6 weeks and 5 micron sections

    Article Snippet: Thirty μg of cellular protein were resolved on SDS polyacrylamid gel (SDS-PAGE) and immunoblotting was performed applying anti-heparanase, anti-VEGF C (Zymed, South San Francisco, CA) and anti-actin (Sigma, St. Louis, MI) antibodies, as described , .

    Techniques: Transfection, Multiple Displacement Amplification, Mouse Assay

    Heparanase expression and HS fragment analysis in the transgenic mouse brain. Immunohistochemistry with the anti-heparanase antibody 63 in tgHpa*Swe and tgSwe mice revealed overexpression of heparanase in tgHpa*Swe mice, particularly in the pyramidal

    Journal: The Journal of Biological Chemistry

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *

    doi: 10.1074/jbc.M114.600569

    Figure Lengend Snippet: Heparanase expression and HS fragment analysis in the transgenic mouse brain. Immunohistochemistry with the anti-heparanase antibody 63 in tgHpa*Swe and tgSwe mice revealed overexpression of heparanase in tgHpa*Swe mice, particularly in the pyramidal

    Article Snippet: 2 μg/ml donkey anti-rabbit Alexa Fluor 594 (catalog no. A-21207, Invitrogen) was used for detecting the anti-heparanase antibody, whereas 2 μg/ml donkey anti-goat Alexa Fluor 488 (catalog no. A-11055, Invitrogen) was used for detecting Iba1.

    Techniques: Expressing, Transgenic Assay, Immunohistochemistry, Mouse Assay, Over Expression

    Neuronal heparanase expression in the tgHpa*Swe mouse brain. Immunofluorescence with the anti-heparanase antibody anti-8 ( green ) and anti-neuronal nuclei antibody ( red ) in the brains of 15-month-old single-transgenic tgSwe ( A–C ) and double-transgenic

    Journal: The Journal of Biological Chemistry

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *

    doi: 10.1074/jbc.M114.600569

    Figure Lengend Snippet: Neuronal heparanase expression in the tgHpa*Swe mouse brain. Immunofluorescence with the anti-heparanase antibody anti-8 ( green ) and anti-neuronal nuclei antibody ( red ) in the brains of 15-month-old single-transgenic tgSwe ( A–C ) and double-transgenic

    Article Snippet: 2 μg/ml donkey anti-rabbit Alexa Fluor 594 (catalog no. A-21207, Invitrogen) was used for detecting the anti-heparanase antibody, whereas 2 μg/ml donkey anti-goat Alexa Fluor 488 (catalog no. A-11055, Invitrogen) was used for detecting Iba1.

    Techniques: Expressing, Immunofluorescence, Transgenic Assay

    Astrocytic heparanase expression in tgHpa*Swe mice. Immunofluorescent detection of positive staining with Methoxy-04 ( blue ), anti-GFAP ( red ), and anti-heparanase ( green ) antibodies on sections in the cerebral cortex from 15-month-old single-transgenic

    Journal: The Journal of Biological Chemistry

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *

    doi: 10.1074/jbc.M114.600569

    Figure Lengend Snippet: Astrocytic heparanase expression in tgHpa*Swe mice. Immunofluorescent detection of positive staining with Methoxy-04 ( blue ), anti-GFAP ( red ), and anti-heparanase ( green ) antibodies on sections in the cerebral cortex from 15-month-old single-transgenic

    Article Snippet: 2 μg/ml donkey anti-rabbit Alexa Fluor 594 (catalog no. A-21207, Invitrogen) was used for detecting the anti-heparanase antibody, whereas 2 μg/ml donkey anti-goat Alexa Fluor 488 (catalog no. A-11055, Invitrogen) was used for detecting Iba1.

    Techniques: Expressing, Mouse Assay, Staining, Transgenic Assay