anti hdac1 5356 mouse monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of <t>HDAC1</t> and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hdac1 5356 mouse monoclonal antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hdac1 5356 mouse monoclonal antibody - by Bioz Stars, 2023-03
    96/100 stars

    Images

    1) Product Images from "CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells"

    Article Title: CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

    Journal: Journal of Clinical Biochemistry and Nutrition

    doi: 10.3164/jcbn.16-109

    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Figure Legend Snippet: Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.
    Figure Legend Snippet: Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.

    Techniques Used: Binding Assay, Negative Control, Western Blot

    Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.
    Figure Legend Snippet: Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.

    Techniques Used: Expressing

    anti hdac1 5356 mouse monoclonal antibody  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hdac1 5356 mouse monoclonal antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hdac1 5356 mouse monoclonal antibody - by Bioz Stars, 2023-03
    86/100 stars

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    anti hdac1 5356 mouse monoclonal antibody  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of <t>HDAC1</t> and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hdac1 5356 mouse monoclonal antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hdac1 5356 mouse monoclonal antibody - by Bioz Stars, 2023-03
    96/100 stars

    Images

    1) Product Images from "CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells"

    Article Title: CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

    Journal: Journal of Clinical Biochemistry and Nutrition

    doi: 10.3164/jcbn.16-109

    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Figure Legend Snippet: Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.
    Figure Legend Snippet: Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.

    Techniques Used: Binding Assay, Negative Control, Western Blot

    Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.
    Figure Legend Snippet: Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.

    Techniques Used: Expressing

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    Cell Signaling Technology Inc anti hdac1 5356 mouse monoclonal antibody
    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of <t>HDAC1</t> and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.
    Anti Hdac1 5356 Mouse Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hdac1 5356 mouse monoclonal antibody/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hdac1 5356 mouse monoclonal antibody - by Bioz Stars, 2023-03
    96/100 stars
    		  Buy from Supplier

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    Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

    doi: 10.3164/jcbn.16-109

    Figure Lengend Snippet: Effects of CAPE on the expression of HDACs. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, mRNA levels were measured by real-time RT-PCR. Real-time RT-PCR data were normalized using 18S rRNA levels. Data were shown as the mean ± SE ( n = 3). (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the protein expression of HDAC1 and HDAC3 was detected by Western blotting. Values are expressed as fold change relative to the level of HDAC1 or HDAC3 in control cells.

    Article Snippet: An anti-HDAC1 (#5356) mouse monoclonal antibody and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

    doi: 10.3164/jcbn.16-109

    Figure Lengend Snippet: Effects of CAPE on the interaction between MEF2A and HDAC1. (A) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the ChIP assay was performed. Relative binding to the promoter region was expressed as a fold amount over input (2%). Data were shown as the mean ± SE ( n = 4). ** p <0.01, NS, not significant. (B) HRECs were treated with 10 µM CAPE for 24 h. After cells had been treated, the interaction between MEF2A and HDAC1 or MEF2D was assessed by IP. NC, negative control. (C) The phosphorylation of MEF2A threonine 312 (MEF2A phospho T312) was determined by Western blotting. Values (B and C) are expressed as fold change relative to the level of HDAC1 or MEF2D (B), or MEF2A phospho T312 (C) in control cells.

    Article Snippet: An anti-HDAC1 (#5356) mouse monoclonal antibody and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Binding Assay, Negative Control, Western Blot

    Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells

    doi: 10.3164/jcbn.16-109

    Figure Lengend Snippet: Proposed model for the involvement of MEF2A/D and HDAC1 in the CAPE-induced up-regulation of SOD3 expression. Under basal conditions, MEF2A/D-HDAC1 complexes bind to the MEF2 regulatory element (MRE) within the SOD3 promoter region, and inhibit the transcription of SOD3 through histone deacetylation. After HRECs have been treated with CAPE, HDAC1 dissociates from the MEF2A/D heterodimer, which increases the enrichment of acetylated histone H3 within the SOD3 promoter region and induces its expression.

    Article Snippet: An anti-HDAC1 (#5356) mouse monoclonal antibody and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Expressing