# h2b active motif catalog no 39237 (Active Motif)

## Structured Review

H2b Active Motif Catalog No 39237, supplied by Active Motif, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/h2b active motif catalog no 39237/product/Active Motif

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# anti histone h2a h2b dna complex (Davids Biotechnologie)

## Structured Review

Anti Histone H2a H2b Dna Complex, supplied by Davids Biotechnologie, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti histone h2a h2b dna complex/product/Davids Biotechnologie

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# h2b active motif catalog no 39237 (Active Motif)

## Structured Review

H2b Active Motif Catalog No 39237, supplied by Active Motif, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/h2b active motif catalog no 39237/product/Active Motif

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# anti histone 2b (Proteintech)

## Structured Review

Anti Histone 2b, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti histone 2b/product/Proteintech

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "P7C3 suppresses astrocytic senescence to protect dopaminergic neurons: Implication in the mouse model of Parkinson’s disease"

**Article Title: **P7C3 suppresses astrocytic senescence to protect dopaminergic neurons: Implication in the mouse model of Parkinson’s disease

**Journal: **CNS Neuroscience & Therapeutics

**doi: **10.1111/cns.14819

**Figure Legend Snippet:**P7C3 Promoted Sirt3 expression to enhance mitophagy and decrease mitochondrial ROS. (A) Astrocytes were cultured in vitro for 10 or 40 days and then treated with P7C3 (10 μM) for 5 days. The protein levels of SIRT3 and β‐actin were measured using immunoblot analysis. The intensity quantification of SIRT3 relative to β‐actin is shown in the right panel. The values are presented as the mean ± SEM. ** p < 0.01 one‐way ANOVA followed by Dunnett's multiple‐comparisons test. (B) Astrocytes were cultured in vitro for 40 days, the were transfected with control siRNA or Sirt3 siRNA for 48 h, and then exposed to P7C3 for 5 days. The protein levels of LaminB1, p16 Ink4a , SIRT3, and β‐actin were measured using immunoblot analysis. The intensity quantification of LaminB1, p16 Ink4a , SIRT3 relative to β‐actin is shown in the right panel. The values are presented as the mean ± SEM. ** p < 0.01, ns, no significance, one‐way ANOVA followed by Tukey's multiple‐comparisons test. (C) Astrocytes were treated as described in (B), the SA‐β‐gal was stained and the percentage of SA‐β‐gal + cells was shown in the right panel. * p < 0.05, ** p < 0.01 one‐way ANOVA followed by Tukey's multiple‐comparisons test. Scale bar, 50 μm. (D) Astrocytes were treated as described in (B), the protein levels of LC3, p62, Tom20, COXIV, SIRT3 and β‐actin were measured using immunoblot analysis. The intensity quantification of LC3 II, p62, Tom20, COXIV, SIRT3 relative to β‐actin is shown in the right panel. The values are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, ns, no significance, one‐way ANOVA followed by Tukey's multiple‐comparisons test. (E) Astrocytes were treated as described in (B), mitochondrial reactive oxygen species (MitoROS) was detected using MitoSox Red. The relative quantification of MitoROS is shown in the right panel. ** p < 0.01, ns, no significance, one‐way ANOVA followed by Dunnett's multiple‐comparisons test. Scale bar, 10 μm. (F) Astrocytes were treated as described in (A). Immunofluorescence staining was performed with anti‐NRF2 and DAPI, relative NRF2 fluorescence intensity in nuclues is shown in the right panel. * p < 0.05, Student's t ‐test. Scale bar, 5 μm. (G) Astrocytes were treated as described in (A). Then, the cytoplasmic and nuclear fractions were separated using subcellular fractionation methods. The NRF2 protein levels in cytoplasm and nucleus were detected using immunoblot analysis. The quantitative analyses of the relative density of NRF2 to loading controls in cytoplasm (α‐Tubulin) or in the nucleus (Histone 2B) were shown in the right panels. The values were presented as the means ± SEM from three independent experiments. ** p < 0.01, Student's t ‐test. (H) Astrocytes were treated as described in (A), Immunoprecipitation (IP) shows the protein levels of NRF2 and KEAP1‐Flag in astrocytes with indicated treatment. An anti‐Flag antibody was used for IP. 10% total protein used as input. ** p < 0.01, Student's t ‐test. (I) Astrocytes were cultured in vitro for 40 days, then were treated with or without Trigoneline for 24 h, followed by the treatment of P7C3 for 5 days. The protein levels of SIRT3 and β‐actin were measured using immunoblot analysis. The intensity quantification of SIRT3 relative to β‐actin is shown in the right panel. The values are presented as the mean ± SEM. ** p < 0.01, ns, no significance, Student's t ‐test.

**Techniques Used: **Expressing, Cell Culture, In Vitro, Western Blot, Transfection, Control, Staining, Immunofluorescence, Fluorescence, Fractionation, Immunoprecipitation

# anti histone h2b (Danaher Inc)

Danaher Inc is a verified supplier

Danaher Inc manufactures this product

## Structured Review

Anti Histone H2b, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti histone h2b/product/Danaher Inc

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force"

**Article Title: **Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force

**Journal: **Cell reports

**doi: **10.1016/j.celrep.2024.114462

**Figure Legend Snippet:**(A) Schematic of anti-histone-H2B-ab-coated 200-nm FMNP microinjection to a CHO (Chinese hamster ovary) cell nucleus. (B) Applying a sinusoidal cyclic stress after magnetizing 200-nm FMNPs in the y direction and twisting toward the z direction using the magnetic twisting cytometry (MTC). (C) Representative images from 4 different cell nuclei in 4 different rows (the same cell in each row) of anti-H2B-ab-coated 200 nm FMNPs and DHFR (dihydrofolate reductase)-GFP spots or free GFP of CHO cell nucleus. Scale bar, 5 μm. (D) Displacements of anti-H2B-ab-coated FMNPs in the same four CHO cells as in (C). (E) Chromatin and nucleoplasm complex moduli (ratio of applied stress to measured chromatin strain) with FMNP applied stress (5 Pa at 0.3 Hz) in CHO cell nuclei. (F) Chromatin and nucleoplasm storage modulus G′ (G′ = (σ 0 /ε 0 ) cos δ) in CHO cell nuclei. (G) Chromatin and nucleoplasm loss modulus G′′ (G′′ = (σ 0 /ε 0 ) sin δ) in CHO cell nuclei. Data (E, F, and G) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates. See also and .

**Techniques Used: **Microinjection, Cytometry, MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin stretching and complex modulus G of CHO cells in the same chromatin domain for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (B) Chromatin storage and loss modulus in CHO cell for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (C) Chromatin average complex, storage, and loss moduli versus stress frequency on a log-log scale for CHO cells. Data (A and B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin displacements depend on DHFR -GFP spots distance from the anti-H2B-ab-coated FMNP. (B) Chromatin stretching (percentage) for microinjected anti-histone-H2B-ab-coated, anti-IgG-ab-coated, or uncoated magnetic FMNPs or a 4-μm RGD (Arg-Gly-Asp)-coated ferromagnetic bead on CHO cell surface. (C) Representative images of CHO cell bright-field (BF) and Cy3 (red) DHFR fluorescence in Situ hybridization (FISH) (same cell in BF and Cy3 [red] fluorescence image for each condition). Scale bar, 5 μm. (D) Normalized DHFR gene transcription from original data in (C). Data (B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. Data (D) are mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Fluorescence, In Situ Hybridization, MANN-WHITNEY

**Figure Legend Snippet:**(A) Diffusion coefficient of DHFR -GFP on the chromatin 5 min after 0-, 2-, or 10-min stress (5 Pa at 0.3 Hz), 1 h post 2- and 10-min FMNP stress and 5 min after 10-min stress, or 1 h post-10-min stress from a 4-μm RGD-coated bead attached to CHO cell surface with the same stress (5 Pa at 0.3 Hz). (B) Diffusion coefficient of free GFP inside the nucleoplasm (free GFPs <5-μm and >5-μm distance from location of DHFR -GFP spots) 5 min after 0-, 2-, or 10-min stress and 1 h post 2- or 10-min stress from anti-H2B-ab-coated FMNP and 10 min or 1 h post-10-min stress from the 4-μm RGD-coated bead attached to a CHO cell surface. Data are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–6 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images from two different hiPSC nuclei. One cell shown on the left (BF image; white arrow points to the nanoparticle) and the middle left (GFP image) was microinjected with an anti-H2B-ab-coated FMNP; another cell shown on the middle right (BF image; white arrow points to the bead) and the right (GFP image) was bound to a 4-μm RGD-coated magnetic bead on its surface. Scale bars, 5 μm. (B) Diffusion coefficient of RNA polymerase II (RNA Pol II) spots 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. (C) Diffusion coefficient of single RNA Pol II molecule (<0.5 μm distance from RNA Pol II arrow shown in A) 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the microinjected anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. Data (B and C) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of BFs and immunofluorescence (red) in hiPSC nuclei. Scale bar, 5 μm. (B) Normalized RNA Pol II activity in hiPSCs 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated magnetic bead at the cell surface. (C) Representative images of BFs and immunofluorescence of RNA Pol II Ser 2 phosphorylation (red) in CHO cell nuclei. Scale bar, 5 μm. (D) Normalized RNA Pol II activity in CHO cells 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress for the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead at the cell surface. Data (B and D) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Immunofluorescence, Activity Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of CHO cell nuclei after microinjection of an anti-H2B-ab-coated FMNP per cell on polyacrylamide (PA) gel substrates of 1-, 10-, or 20-kPa stiffness. In each inset, an FMNP is within a dashed circle. Scale bar, 5 μm. (B) Diffusion coefficient of DHFR -GFP on the chromatin of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness at 0 or 5 min after 2- or 10-min stress or 1 h post-2-or 10-min stress from an anti-H2B-ab-coated FMNP. (C) Chromatin stretching in CHO cells in response to microinjected anti-H2B-ab-coated FMNP stress on a 1-, 10-, or 20-kPa substrate stiffness. (D) Chromatin complex modulus of CHO cells on 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected anti-H2B-ab-coated FMNP stress. (E) Chromatin storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (F) Chromatin loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (G) Nucleoplasm complex modulus of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected uncoated FMNP stress. (H) Nucleoplasm storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. (I) Nucleoplasm loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. The applied stress was 5 Pa at 0.3 Hz for (B)–(I). Data (B–I) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **Microinjection, Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**KEY RESOURCES TABLE

**Techniques Used: **Recombinant, Membrane, Knock-Out, Software, Inverted Microscopy, Transmission Assay, Microscopy

# anti h2b (MathWorks Inc)

## Structured Review

Anti H2b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti h2b/product/MathWorks Inc

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force"

**Article Title: **Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force

**Journal: **Cell reports

**doi: **10.1016/j.celrep.2024.114462

**Figure Legend Snippet:**(A) Schematic of anti-histone-H2B-ab-coated 200-nm FMNP microinjection to a CHO (Chinese hamster ovary) cell nucleus. (B) Applying a sinusoidal cyclic stress after magnetizing 200-nm FMNPs in the y direction and twisting toward the z direction using the magnetic twisting cytometry (MTC). (C) Representative images from 4 different cell nuclei in 4 different rows (the same cell in each row) of anti-H2B-ab-coated 200 nm FMNPs and DHFR (dihydrofolate reductase)-GFP spots or free GFP of CHO cell nucleus. Scale bar, 5 μm. (D) Displacements of anti-H2B-ab-coated FMNPs in the same four CHO cells as in (C). (E) Chromatin and nucleoplasm complex moduli (ratio of applied stress to measured chromatin strain) with FMNP applied stress (5 Pa at 0.3 Hz) in CHO cell nuclei. (F) Chromatin and nucleoplasm storage modulus G′ (G′ = (σ 0 /ε 0 ) cos δ) in CHO cell nuclei. (G) Chromatin and nucleoplasm loss modulus G′′ (G′′ = (σ 0 /ε 0 ) sin δ) in CHO cell nuclei. Data (E, F, and G) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates. See also and .

**Techniques Used: **Microinjection, Cytometry, MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin stretching and complex modulus G of CHO cells in the same chromatin domain for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (B) Chromatin storage and loss modulus in CHO cell for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (C) Chromatin average complex, storage, and loss moduli versus stress frequency on a log-log scale for CHO cells. Data (A and B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin displacements depend on DHFR -GFP spots distance from the anti-H2B-ab-coated FMNP. (B) Chromatin stretching (percentage) for microinjected anti-histone-H2B-ab-coated, anti-IgG-ab-coated, or uncoated magnetic FMNPs or a 4-μm RGD (Arg-Gly-Asp)-coated ferromagnetic bead on CHO cell surface. (C) Representative images of CHO cell bright-field (BF) and Cy3 (red) DHFR fluorescence in Situ hybridization (FISH) (same cell in BF and Cy3 [red] fluorescence image for each condition). Scale bar, 5 μm. (D) Normalized DHFR gene transcription from original data in (C). Data (B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. Data (D) are mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Fluorescence, In Situ Hybridization, MANN-WHITNEY

**Figure Legend Snippet:**(A) Diffusion coefficient of DHFR -GFP on the chromatin 5 min after 0-, 2-, or 10-min stress (5 Pa at 0.3 Hz), 1 h post 2- and 10-min FMNP stress and 5 min after 10-min stress, or 1 h post-10-min stress from a 4-μm RGD-coated bead attached to CHO cell surface with the same stress (5 Pa at 0.3 Hz). (B) Diffusion coefficient of free GFP inside the nucleoplasm (free GFPs <5-μm and >5-μm distance from location of DHFR -GFP spots) 5 min after 0-, 2-, or 10-min stress and 1 h post 2- or 10-min stress from anti-H2B-ab-coated FMNP and 10 min or 1 h post-10-min stress from the 4-μm RGD-coated bead attached to a CHO cell surface. Data are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–6 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images from two different hiPSC nuclei. One cell shown on the left (BF image; white arrow points to the nanoparticle) and the middle left (GFP image) was microinjected with an anti-H2B-ab-coated FMNP; another cell shown on the middle right (BF image; white arrow points to the bead) and the right (GFP image) was bound to a 4-μm RGD-coated magnetic bead on its surface. Scale bars, 5 μm. (B) Diffusion coefficient of RNA polymerase II (RNA Pol II) spots 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. (C) Diffusion coefficient of single RNA Pol II molecule (<0.5 μm distance from RNA Pol II arrow shown in A) 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the microinjected anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. Data (B and C) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of BFs and immunofluorescence (red) in hiPSC nuclei. Scale bar, 5 μm. (B) Normalized RNA Pol II activity in hiPSCs 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated magnetic bead at the cell surface. (C) Representative images of BFs and immunofluorescence of RNA Pol II Ser 2 phosphorylation (red) in CHO cell nuclei. Scale bar, 5 μm. (D) Normalized RNA Pol II activity in CHO cells 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress for the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead at the cell surface. Data (B and D) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Immunofluorescence, Activity Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of CHO cell nuclei after microinjection of an anti-H2B-ab-coated FMNP per cell on polyacrylamide (PA) gel substrates of 1-, 10-, or 20-kPa stiffness. In each inset, an FMNP is within a dashed circle. Scale bar, 5 μm. (B) Diffusion coefficient of DHFR -GFP on the chromatin of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness at 0 or 5 min after 2- or 10-min stress or 1 h post-2-or 10-min stress from an anti-H2B-ab-coated FMNP. (C) Chromatin stretching in CHO cells in response to microinjected anti-H2B-ab-coated FMNP stress on a 1-, 10-, or 20-kPa substrate stiffness. (D) Chromatin complex modulus of CHO cells on 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected anti-H2B-ab-coated FMNP stress. (E) Chromatin storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (F) Chromatin loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (G) Nucleoplasm complex modulus of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected uncoated FMNP stress. (H) Nucleoplasm storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. (I) Nucleoplasm loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. The applied stress was 5 Pa at 0.3 Hz for (B)–(I). Data (B–I) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **Microinjection, Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**KEY RESOURCES TABLE

**Techniques Used: **Recombinant, Membrane, Knock-Out, Software, Inverted Microscopy, Transmission Assay, Microscopy

# anti histone h2b antibody (Danaher Inc)

Danaher Inc is a verified supplier

Danaher Inc manufactures this product

## Structured Review

Anti Histone H2b Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti histone h2b antibody/product/Danaher Inc

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force"

**Article Title: **Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force

**Journal: **Cell reports

**doi: **10.1016/j.celrep.2024.114462

**Figure Legend Snippet:**(A) Schematic of anti-histone-H2B-ab-coated 200-nm FMNP microinjection to a CHO (Chinese hamster ovary) cell nucleus. (B) Applying a sinusoidal cyclic stress after magnetizing 200-nm FMNPs in the y direction and twisting toward the z direction using the magnetic twisting cytometry (MTC). (C) Representative images from 4 different cell nuclei in 4 different rows (the same cell in each row) of anti-H2B-ab-coated 200 nm FMNPs and DHFR (dihydrofolate reductase)-GFP spots or free GFP of CHO cell nucleus. Scale bar, 5 μm. (D) Displacements of anti-H2B-ab-coated FMNPs in the same four CHO cells as in (C). (E) Chromatin and nucleoplasm complex moduli (ratio of applied stress to measured chromatin strain) with FMNP applied stress (5 Pa at 0.3 Hz) in CHO cell nuclei. (F) Chromatin and nucleoplasm storage modulus G′ (G′ = (σ 0 /ε 0 ) cos δ) in CHO cell nuclei. (G) Chromatin and nucleoplasm loss modulus G′′ (G′′ = (σ 0 /ε 0 ) sin δ) in CHO cell nuclei. Data (E, F, and G) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates. See also and .

**Techniques Used: **Microinjection, Cytometry, MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin stretching and complex modulus G of CHO cells in the same chromatin domain for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (B) Chromatin storage and loss modulus in CHO cell for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (C) Chromatin average complex, storage, and loss moduli versus stress frequency on a log-log scale for CHO cells. Data (A and B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin displacements depend on DHFR -GFP spots distance from the anti-H2B-ab-coated FMNP. (B) Chromatin stretching (percentage) for microinjected anti-histone-H2B-ab-coated, anti-IgG-ab-coated, or uncoated magnetic FMNPs or a 4-μm RGD (Arg-Gly-Asp)-coated ferromagnetic bead on CHO cell surface. (C) Representative images of CHO cell bright-field (BF) and Cy3 (red) DHFR fluorescence in Situ hybridization (FISH) (same cell in BF and Cy3 [red] fluorescence image for each condition). Scale bar, 5 μm. (D) Normalized DHFR gene transcription from original data in (C). Data (B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. Data (D) are mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Fluorescence, In Situ Hybridization, MANN-WHITNEY

**Figure Legend Snippet:**(A) Diffusion coefficient of DHFR -GFP on the chromatin 5 min after 0-, 2-, or 10-min stress (5 Pa at 0.3 Hz), 1 h post 2- and 10-min FMNP stress and 5 min after 10-min stress, or 1 h post-10-min stress from a 4-μm RGD-coated bead attached to CHO cell surface with the same stress (5 Pa at 0.3 Hz). (B) Diffusion coefficient of free GFP inside the nucleoplasm (free GFPs <5-μm and >5-μm distance from location of DHFR -GFP spots) 5 min after 0-, 2-, or 10-min stress and 1 h post 2- or 10-min stress from anti-H2B-ab-coated FMNP and 10 min or 1 h post-10-min stress from the 4-μm RGD-coated bead attached to a CHO cell surface. Data are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–6 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images from two different hiPSC nuclei. One cell shown on the left (BF image; white arrow points to the nanoparticle) and the middle left (GFP image) was microinjected with an anti-H2B-ab-coated FMNP; another cell shown on the middle right (BF image; white arrow points to the bead) and the right (GFP image) was bound to a 4-μm RGD-coated magnetic bead on its surface. Scale bars, 5 μm. (B) Diffusion coefficient of RNA polymerase II (RNA Pol II) spots 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. (C) Diffusion coefficient of single RNA Pol II molecule (<0.5 μm distance from RNA Pol II arrow shown in A) 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the microinjected anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. Data (B and C) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of BFs and immunofluorescence (red) in hiPSC nuclei. Scale bar, 5 μm. (B) Normalized RNA Pol II activity in hiPSCs 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated magnetic bead at the cell surface. (C) Representative images of BFs and immunofluorescence of RNA Pol II Ser 2 phosphorylation (red) in CHO cell nuclei. Scale bar, 5 μm. (D) Normalized RNA Pol II activity in CHO cells 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress for the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead at the cell surface. Data (B and D) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Immunofluorescence, Activity Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of CHO cell nuclei after microinjection of an anti-H2B-ab-coated FMNP per cell on polyacrylamide (PA) gel substrates of 1-, 10-, or 20-kPa stiffness. In each inset, an FMNP is within a dashed circle. Scale bar, 5 μm. (B) Diffusion coefficient of DHFR -GFP on the chromatin of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness at 0 or 5 min after 2- or 10-min stress or 1 h post-2-or 10-min stress from an anti-H2B-ab-coated FMNP. (C) Chromatin stretching in CHO cells in response to microinjected anti-H2B-ab-coated FMNP stress on a 1-, 10-, or 20-kPa substrate stiffness. (D) Chromatin complex modulus of CHO cells on 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected anti-H2B-ab-coated FMNP stress. (E) Chromatin storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (F) Chromatin loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (G) Nucleoplasm complex modulus of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected uncoated FMNP stress. (H) Nucleoplasm storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. (I) Nucleoplasm loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. The applied stress was 5 Pa at 0.3 Hz for (B)–(I). Data (B–I) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **Microinjection, Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**KEY RESOURCES TABLE

**Techniques Used: **Recombinant, Membrane, Knock-Out, Software, Inverted Microscopy, Transmission Assay, Microscopy

# anti h2b ab (Santa Cruz Biotechnology)

## Structured Review

Anti H2b Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti h2b ab/product/Santa Cruz Biotechnology

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

**Article Title: **Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force

**Journal: **Cell reports

**doi: **10.1016/j.celrep.2024.114462

**Figure Legend Snippet:**(A) Schematic of anti-histone-H2B-ab-coated 200-nm FMNP microinjection to a CHO (Chinese hamster ovary) cell nucleus. (B) Applying a sinusoidal cyclic stress after magnetizing 200-nm FMNPs in the y direction and twisting toward the z direction using the magnetic twisting cytometry (MTC). (C) Representative images from 4 different cell nuclei in 4 different rows (the same cell in each row) of anti-H2B-ab-coated 200 nm FMNPs and DHFR (dihydrofolate reductase)-GFP spots or free GFP of CHO cell nucleus. Scale bar, 5 μm. (D) Displacements of anti-H2B-ab-coated FMNPs in the same four CHO cells as in (C). (E) Chromatin and nucleoplasm complex moduli (ratio of applied stress to measured chromatin strain) with FMNP applied stress (5 Pa at 0.3 Hz) in CHO cell nuclei. (F) Chromatin and nucleoplasm storage modulus G′ (G′ = (σ 0 /ε 0 ) cos δ) in CHO cell nuclei. (G) Chromatin and nucleoplasm loss modulus G′′ (G′′ = (σ 0 /ε 0 ) sin δ) in CHO cell nuclei. Data (E, F, and G) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates. See also and .

**Techniques Used: **Microinjection, Cytometry, MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin stretching and complex modulus G of CHO cells in the same chromatin domain for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (B) Chromatin storage and loss modulus in CHO cell for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (C) Chromatin average complex, storage, and loss moduli versus stress frequency on a log-log scale for CHO cells. Data (A and B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin displacements depend on DHFR -GFP spots distance from the anti-H2B-ab-coated FMNP. (B) Chromatin stretching (percentage) for microinjected anti-histone-H2B-ab-coated, anti-IgG-ab-coated, or uncoated magnetic FMNPs or a 4-μm RGD (Arg-Gly-Asp)-coated ferromagnetic bead on CHO cell surface. (C) Representative images of CHO cell bright-field (BF) and Cy3 (red) DHFR fluorescence in Situ hybridization (FISH) (same cell in BF and Cy3 [red] fluorescence image for each condition). Scale bar, 5 μm. (D) Normalized DHFR gene transcription from original data in (C). Data (B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. Data (D) are mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Fluorescence, In Situ Hybridization, MANN-WHITNEY

**Figure Legend Snippet:**(A) Diffusion coefficient of DHFR -GFP on the chromatin 5 min after 0-, 2-, or 10-min stress (5 Pa at 0.3 Hz), 1 h post 2- and 10-min FMNP stress and 5 min after 10-min stress, or 1 h post-10-min stress from a 4-μm RGD-coated bead attached to CHO cell surface with the same stress (5 Pa at 0.3 Hz). (B) Diffusion coefficient of free GFP inside the nucleoplasm (free GFPs <5-μm and >5-μm distance from location of DHFR -GFP spots) 5 min after 0-, 2-, or 10-min stress and 1 h post 2- or 10-min stress from anti-H2B-ab-coated FMNP and 10 min or 1 h post-10-min stress from the 4-μm RGD-coated bead attached to a CHO cell surface. Data are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–6 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images from two different hiPSC nuclei. One cell shown on the left (BF image; white arrow points to the nanoparticle) and the middle left (GFP image) was microinjected with an anti-H2B-ab-coated FMNP; another cell shown on the middle right (BF image; white arrow points to the bead) and the right (GFP image) was bound to a 4-μm RGD-coated magnetic bead on its surface. Scale bars, 5 μm. (B) Diffusion coefficient of RNA polymerase II (RNA Pol II) spots 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. (C) Diffusion coefficient of single RNA Pol II molecule (<0.5 μm distance from RNA Pol II arrow shown in A) 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the microinjected anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. Data (B and C) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of BFs and immunofluorescence (red) in hiPSC nuclei. Scale bar, 5 μm. (B) Normalized RNA Pol II activity in hiPSCs 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated magnetic bead at the cell surface. (C) Representative images of BFs and immunofluorescence of RNA Pol II Ser 2 phosphorylation (red) in CHO cell nuclei. Scale bar, 5 μm. (D) Normalized RNA Pol II activity in CHO cells 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress for the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead at the cell surface. Data (B and D) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Immunofluorescence, Activity Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of CHO cell nuclei after microinjection of an anti-H2B-ab-coated FMNP per cell on polyacrylamide (PA) gel substrates of 1-, 10-, or 20-kPa stiffness. In each inset, an FMNP is within a dashed circle. Scale bar, 5 μm. (B) Diffusion coefficient of DHFR -GFP on the chromatin of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness at 0 or 5 min after 2- or 10-min stress or 1 h post-2-or 10-min stress from an anti-H2B-ab-coated FMNP. (C) Chromatin stretching in CHO cells in response to microinjected anti-H2B-ab-coated FMNP stress on a 1-, 10-, or 20-kPa substrate stiffness. (D) Chromatin complex modulus of CHO cells on 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected anti-H2B-ab-coated FMNP stress. (E) Chromatin storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (F) Chromatin loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (G) Nucleoplasm complex modulus of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected uncoated FMNP stress. (H) Nucleoplasm storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. (I) Nucleoplasm loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. The applied stress was 5 Pa at 0.3 Hz for (B)–(I). Data (B–I) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **Microinjection, Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**KEY RESOURCES TABLE

**Techniques Used: **Recombinant, Membrane, Knock-Out, Software, Inverted Microscopy, Transmission Assay, Microscopy

# anti h2b (Santa Cruz Biotechnology)

## Structured Review

Anti H2b, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti h2b/product/Santa Cruz Biotechnology

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

**Article Title: **Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force

**Journal: **Cell reports

**doi: **10.1016/j.celrep.2024.114462

**Figure Legend Snippet:**(A) Schematic of anti-histone-H2B-ab-coated 200-nm FMNP microinjection to a CHO (Chinese hamster ovary) cell nucleus. (B) Applying a sinusoidal cyclic stress after magnetizing 200-nm FMNPs in the y direction and twisting toward the z direction using the magnetic twisting cytometry (MTC). (C) Representative images from 4 different cell nuclei in 4 different rows (the same cell in each row) of anti-H2B-ab-coated 200 nm FMNPs and DHFR (dihydrofolate reductase)-GFP spots or free GFP of CHO cell nucleus. Scale bar, 5 μm. (D) Displacements of anti-H2B-ab-coated FMNPs in the same four CHO cells as in (C). (E) Chromatin and nucleoplasm complex moduli (ratio of applied stress to measured chromatin strain) with FMNP applied stress (5 Pa at 0.3 Hz) in CHO cell nuclei. (F) Chromatin and nucleoplasm storage modulus G′ (G′ = (σ 0 /ε 0 ) cos δ) in CHO cell nuclei. (G) Chromatin and nucleoplasm loss modulus G′′ (G′′ = (σ 0 /ε 0 ) sin δ) in CHO cell nuclei. Data (E, F, and G) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates. See also and .

**Techniques Used: **Microinjection, Cytometry, MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin stretching and complex modulus G of CHO cells in the same chromatin domain for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (B) Chromatin storage and loss modulus in CHO cell for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (C) Chromatin average complex, storage, and loss moduli versus stress frequency on a log-log scale for CHO cells. Data (A and B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin displacements depend on DHFR -GFP spots distance from the anti-H2B-ab-coated FMNP. (B) Chromatin stretching (percentage) for microinjected anti-histone-H2B-ab-coated, anti-IgG-ab-coated, or uncoated magnetic FMNPs or a 4-μm RGD (Arg-Gly-Asp)-coated ferromagnetic bead on CHO cell surface. (C) Representative images of CHO cell bright-field (BF) and Cy3 (red) DHFR fluorescence in Situ hybridization (FISH) (same cell in BF and Cy3 [red] fluorescence image for each condition). Scale bar, 5 μm. (D) Normalized DHFR gene transcription from original data in (C). Data (B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. Data (D) are mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Fluorescence, In Situ Hybridization, MANN-WHITNEY

**Figure Legend Snippet:**(A) Diffusion coefficient of DHFR -GFP on the chromatin 5 min after 0-, 2-, or 10-min stress (5 Pa at 0.3 Hz), 1 h post 2- and 10-min FMNP stress and 5 min after 10-min stress, or 1 h post-10-min stress from a 4-μm RGD-coated bead attached to CHO cell surface with the same stress (5 Pa at 0.3 Hz). (B) Diffusion coefficient of free GFP inside the nucleoplasm (free GFPs <5-μm and >5-μm distance from location of DHFR -GFP spots) 5 min after 0-, 2-, or 10-min stress and 1 h post 2- or 10-min stress from anti-H2B-ab-coated FMNP and 10 min or 1 h post-10-min stress from the 4-μm RGD-coated bead attached to a CHO cell surface. Data are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–6 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images from two different hiPSC nuclei. One cell shown on the left (BF image; white arrow points to the nanoparticle) and the middle left (GFP image) was microinjected with an anti-H2B-ab-coated FMNP; another cell shown on the middle right (BF image; white arrow points to the bead) and the right (GFP image) was bound to a 4-μm RGD-coated magnetic bead on its surface. Scale bars, 5 μm. (B) Diffusion coefficient of RNA polymerase II (RNA Pol II) spots 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. (C) Diffusion coefficient of single RNA Pol II molecule (<0.5 μm distance from RNA Pol II arrow shown in A) 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the microinjected anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. Data (B and C) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of BFs and immunofluorescence (red) in hiPSC nuclei. Scale bar, 5 μm. (B) Normalized RNA Pol II activity in hiPSCs 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated magnetic bead at the cell surface. (C) Representative images of BFs and immunofluorescence of RNA Pol II Ser 2 phosphorylation (red) in CHO cell nuclei. Scale bar, 5 μm. (D) Normalized RNA Pol II activity in CHO cells 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress for the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead at the cell surface. Data (B and D) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Immunofluorescence, Activity Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of CHO cell nuclei after microinjection of an anti-H2B-ab-coated FMNP per cell on polyacrylamide (PA) gel substrates of 1-, 10-, or 20-kPa stiffness. In each inset, an FMNP is within a dashed circle. Scale bar, 5 μm. (B) Diffusion coefficient of DHFR -GFP on the chromatin of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness at 0 or 5 min after 2- or 10-min stress or 1 h post-2-or 10-min stress from an anti-H2B-ab-coated FMNP. (C) Chromatin stretching in CHO cells in response to microinjected anti-H2B-ab-coated FMNP stress on a 1-, 10-, or 20-kPa substrate stiffness. (D) Chromatin complex modulus of CHO cells on 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected anti-H2B-ab-coated FMNP stress. (E) Chromatin storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (F) Chromatin loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (G) Nucleoplasm complex modulus of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected uncoated FMNP stress. (H) Nucleoplasm storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. (I) Nucleoplasm loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. The applied stress was 5 Pa at 0.3 Hz for (B)–(I). Data (B–I) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **Microinjection, Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**KEY RESOURCES TABLE

**Techniques Used: **Recombinant, Membrane, Knock-Out, Software, Inverted Microscopy, Transmission Assay, Microscopy

# anti histone h2b (Eppendorf AG)

## Structured Review

Anti Histone H2b, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/anti histone h2b/product/Eppendorf AG

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

**Article Title: **Mechanomemory of nucleoplasm and RNA polymerase II after chromatin stretching by a microinjected magnetic nanoparticle force

**Journal: **Cell reports

**doi: **10.1016/j.celrep.2024.114462

**Figure Legend Snippet:**(A) Schematic of anti-histone-H2B-ab-coated 200-nm FMNP microinjection to a CHO (Chinese hamster ovary) cell nucleus. (B) Applying a sinusoidal cyclic stress after magnetizing 200-nm FMNPs in the y direction and twisting toward the z direction using the magnetic twisting cytometry (MTC). (C) Representative images from 4 different cell nuclei in 4 different rows (the same cell in each row) of anti-H2B-ab-coated 200 nm FMNPs and DHFR (dihydrofolate reductase)-GFP spots or free GFP of CHO cell nucleus. Scale bar, 5 μm. (D) Displacements of anti-H2B-ab-coated FMNPs in the same four CHO cells as in (C). (E) Chromatin and nucleoplasm complex moduli (ratio of applied stress to measured chromatin strain) with FMNP applied stress (5 Pa at 0.3 Hz) in CHO cell nuclei. (F) Chromatin and nucleoplasm storage modulus G′ (G′ = (σ 0 /ε 0 ) cos δ) in CHO cell nuclei. (G) Chromatin and nucleoplasm loss modulus G′′ (G′′ = (σ 0 /ε 0 ) sin δ) in CHO cell nuclei. Data (E, F, and G) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates. See also and .

**Techniques Used: **Microinjection, Cytometry, MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin stretching and complex modulus G of CHO cells in the same chromatin domain for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (B) Chromatin storage and loss modulus in CHO cell for anti-H2B-ab-coated FMNP with applied stress 5 Pa at 0.1, 0.3, 0.8, or 5 Hz. (C) Chromatin average complex, storage, and loss moduli versus stress frequency on a log-log scale for CHO cells. Data (A and B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **MANN-WHITNEY

**Figure Legend Snippet:**(A) Chromatin displacements depend on DHFR -GFP spots distance from the anti-H2B-ab-coated FMNP. (B) Chromatin stretching (percentage) for microinjected anti-histone-H2B-ab-coated, anti-IgG-ab-coated, or uncoated magnetic FMNPs or a 4-μm RGD (Arg-Gly-Asp)-coated ferromagnetic bead on CHO cell surface. (C) Representative images of CHO cell bright-field (BF) and Cy3 (red) DHFR fluorescence in Situ hybridization (FISH) (same cell in BF and Cy3 [red] fluorescence image for each condition). Scale bar, 5 μm. (D) Normalized DHFR gene transcription from original data in (C). Data (B) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. Data (D) are mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Fluorescence, In Situ Hybridization, MANN-WHITNEY

**Figure Legend Snippet:**(A) Diffusion coefficient of DHFR -GFP on the chromatin 5 min after 0-, 2-, or 10-min stress (5 Pa at 0.3 Hz), 1 h post 2- and 10-min FMNP stress and 5 min after 10-min stress, or 1 h post-10-min stress from a 4-μm RGD-coated bead attached to CHO cell surface with the same stress (5 Pa at 0.3 Hz). (B) Diffusion coefficient of free GFP inside the nucleoplasm (free GFPs <5-μm and >5-μm distance from location of DHFR -GFP spots) 5 min after 0-, 2-, or 10-min stress and 1 h post 2- or 10-min stress from anti-H2B-ab-coated FMNP and 10 min or 1 h post-10-min stress from the 4-μm RGD-coated bead attached to a CHO cell surface. Data are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–6 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images from two different hiPSC nuclei. One cell shown on the left (BF image; white arrow points to the nanoparticle) and the middle left (GFP image) was microinjected with an anti-H2B-ab-coated FMNP; another cell shown on the middle right (BF image; white arrow points to the bead) and the right (GFP image) was bound to a 4-μm RGD-coated magnetic bead on its surface. Scale bars, 5 μm. (B) Diffusion coefficient of RNA polymerase II (RNA Pol II) spots 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. (C) Diffusion coefficient of single RNA Pol II molecule (<0.5 μm distance from RNA Pol II arrow shown in A) 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the microinjected anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead on the hiPSC surface. Data (B and C) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of BFs and immunofluorescence (red) in hiPSC nuclei. Scale bar, 5 μm. (B) Normalized RNA Pol II activity in hiPSCs 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress from the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated magnetic bead at the cell surface. (C) Representative images of BFs and immunofluorescence of RNA Pol II Ser 2 phosphorylation (red) in CHO cell nuclei. Scale bar, 5 μm. (D) Normalized RNA Pol II activity in CHO cells 5 min after 0-, 2-, or 10-min stress and 1 h post-2- or 10-min stress for the anti-H2B-ab-coated FMNP and the 4-μm RGD-coated bead at the cell surface. Data (B and D) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3–4 biological replicates. See also .

**Techniques Used: **Immunofluorescence, Activity Assay, MANN-WHITNEY

**Figure Legend Snippet:**(A) Representative images of CHO cell nuclei after microinjection of an anti-H2B-ab-coated FMNP per cell on polyacrylamide (PA) gel substrates of 1-, 10-, or 20-kPa stiffness. In each inset, an FMNP is within a dashed circle. Scale bar, 5 μm. (B) Diffusion coefficient of DHFR -GFP on the chromatin of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness at 0 or 5 min after 2- or 10-min stress or 1 h post-2-or 10-min stress from an anti-H2B-ab-coated FMNP. (C) Chromatin stretching in CHO cells in response to microinjected anti-H2B-ab-coated FMNP stress on a 1-, 10-, or 20-kPa substrate stiffness. (D) Chromatin complex modulus of CHO cells on 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected anti-H2B-ab-coated FMNP stress. (E) Chromatin storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (F) Chromatin loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the anti-H2B-ab-coated FMNP stress. (G) Nucleoplasm complex modulus of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to a microinjected uncoated FMNP stress. (H) Nucleoplasm storage modulus (G′ = (σ 0 /ε 0 ) cos δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. (I) Nucleoplasm loss modulus (G′′ = (σ 0 /ε 0 ) sin δ) of CHO cells on a 1-, 10-, or 20-kPa substrate stiffness in response to the uncoated FMNP stress. The applied stress was 5 Pa at 0.3 Hz for (B)–(I). Data (B–I) are boxplots with minimum, 5% percentile, 25% percentile, median, 75% percentile, maximum, and mean. * p < 0.05, ** p < 0.01, and *** p < 0.001, ns = not statistically different using one-way ANOVA with Tukey and Mann-Whitney tests. n = 3 biological replicates.

**Techniques Used: **Microinjection, Diffusion-based Assay, MANN-WHITNEY

**Figure Legend Snippet:**KEY RESOURCES TABLE

**Techniques Used: **Recombinant, Membrane, Knock-Out, Software, Inverted Microscopy, Transmission Assay, Microscopy