h2b k120ub rabbit monoclonal anti h2b k120ub  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc h2b k120ub rabbit monoclonal anti h2b k120ub
    H2b K120ub Rabbit Monoclonal Anti H2b K120ub, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2b k120ub rabbit monoclonal anti h2b k120ub/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2b k120ub rabbit monoclonal anti h2b k120ub - by Bioz Stars, 2024-06
    86/100 stars

    Images

    anti h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti h2b
    Anti H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h2b - by Bioz Stars, 2024-06
    86/100 stars

    Images

    anti h2b 2934s  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti h2b 2934s
    A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and <t>H2B</t> antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.
    Anti H2b 2934s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2b 2934s/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h2b 2934s - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "RAD18 O-GlcNAcylation promotes translesion DNA synthesis and homologous recombination repair"

    Article Title: RAD18 O-GlcNAcylation promotes translesion DNA synthesis and homologous recombination repair

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06700-y

    A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and H2B antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.
    Figure Legend Snippet: A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and H2B antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.

    Techniques Used: Staining, Irradiation, Western Blot, Transfection, Construct, Expressing

    h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc h2b
    H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2b - by Bioz Stars, 2024-06
    86/100 stars

    Images

    h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc h2b
    H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2b - by Bioz Stars, 2024-06
    86/100 stars

    Images

    anti h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti h2b
    Anti H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h2b - by Bioz Stars, 2024-06
    86/100 stars

    Images

    ubiquityl histone h2b lys120 d11 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc ubiquityl histone h2b lys120 d11 rabbit mab
    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and <t>H2B.</t> Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.
    Ubiquityl Histone H2b Lys120 D11 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubiquityl histone h2b lys120 d11 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ubiquityl histone h2b lys120 d11 rabbit mab - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Structure of the human Bre1 complex bound to the nucleosome"

    Article Title: Structure of the human Bre1 complex bound to the nucleosome

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46910-8

    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and H2B. Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.
    Figure Legend Snippet: a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and H2B. Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.

    Techniques Used: Binding Assay

    nb100 2247 rrid ab 2238596 h2b k120ub1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc nb100 2247 rrid ab 2238596 h2b k120ub1
    Nb100 2247 Rrid Ab 2238596 H2b K120ub1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb100 2247 rrid ab 2238596 h2b k120ub1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nb100 2247 rrid ab 2238596 h2b k120ub1 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    ubiquityl h2b  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc ubiquityl h2b
    Ubiquityl H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubiquityl h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ubiquityl h2b - by Bioz Stars, 2024-06
    86/100 stars

    Images

    anti ubiquityl histone h2b lys120  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc anti ubiquityl histone h2b lys120
    Anti Ubiquityl Histone H2b Lys120, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ubiquityl histone h2b lys120/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ubiquityl histone h2b lys120 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Cell Signaling Technology Inc h2b k120ub rabbit monoclonal anti h2b k120ub
    H2b K120ub Rabbit Monoclonal Anti H2b K120ub, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2b k120ub rabbit monoclonal anti h2b k120ub/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2b k120ub rabbit monoclonal anti h2b k120ub - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti h2b
    Anti H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h2b - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti h2b 2934s
    A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and <t>H2B</t> antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.
    Anti H2b 2934s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2b 2934s/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h2b 2934s - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc h2b
    A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and <t>H2B</t> antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.
    H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2b - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc ubiquityl histone h2b lys120 d11 rabbit mab
    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and <t>H2B.</t> Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.
    Ubiquityl Histone H2b Lys120 D11 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubiquityl histone h2b lys120 d11 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ubiquityl histone h2b lys120 d11 rabbit mab - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc nb100 2247 rrid ab 2238596 h2b k120ub1
    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and <t>H2B.</t> Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.
    Nb100 2247 Rrid Ab 2238596 H2b K120ub1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb100 2247 rrid ab 2238596 h2b k120ub1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nb100 2247 rrid ab 2238596 h2b k120ub1 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc ubiquityl h2b
    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and <t>H2B.</t> Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.
    Ubiquityl H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubiquityl h2b/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ubiquityl h2b - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti ubiquityl histone h2b lys120
    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and <t>H2B.</t> Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.
    Anti Ubiquityl Histone H2b Lys120, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ubiquityl histone h2b lys120/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ubiquityl histone h2b lys120 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and H2B antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.

    Journal: Cell Death & Disease

    Article Title: RAD18 O-GlcNAcylation promotes translesion DNA synthesis and homologous recombination repair

    doi: 10.1038/s41419-024-06700-y

    Figure Lengend Snippet: A Representative image for the dynamic recruitment of WT or 3A GFP-RAD18 to laser-induced DSBs. Data were presented as mean ± SEM from 15 cells. Scale bar, 10 μm. B Quantification of the time course of WT or 3A GFP-RAD18 recruitment after laser microirradiation. C Representative images of GFP-RAD18 foci stained with DAPI after UV irradiation. Scale bars: 2 μm. The protein levels of RAD18 in RAD18+/+ and RAD18-/- U2OS cells were detected by immunoblotting. D Quantification of the percentage of RAD18-/- U2OS cells transfected with WT or 3A GFP-RAD18 constructs with more than 30 RAD18 foci after CPT, Bleomycin and UV exposure by counting at least 200 cells in each experiment. Data represent means ± SEM from three independent experiments. E Chromatin fractions of HEK293T cells expressing WT or 3A SFB-RAD18 were extracted followed by immunoblotting with Flag and H2B antibodies. The whole cell extract (WCE) was harvested and immunoblotted with Flag and β-actin antibodies.

    Article Snippet: Antibodies sources were as follows: mouse anti-Flag (F1804, 1:1000) from Sigma (St Louis, MO), anti-RAD18 (ab17725, 1:1000) from Abcam and (H00056852-M01) from Novus Biologicals, anti-RAD18 pS434 from Dia-An Biotechnology, O-linked β-N-acetylglucosamine (O-GlcNAc, ab2739, 1:2000) and anti-RAD51 (ab133534, 1:200) from Abcam, anti-HA (902302, 1:2000) from BioLegend, anti-Myc (MMS-150R-500, 1:1000) from Covance, anti-H3.1 (P30266, 1:2000) from Abmart, anti-β-Tubulin (AbM59005-37-PU, 1:4000) from Beijing Protein Innovation (Beijing, China), anti-GFP (sc-8334, 1:500), OGT (sc-32921, 1:1000) and anti-PCNA (sc-56, 1:1000) from Santa Cruz Biotechnology, anti-H2B (2934S) from Cell Signaling Technology (CST).

    Techniques: Staining, Irradiation, Western Blot, Transfection, Construct, Expressing

    a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and H2B. Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.

    Journal: Nature Communications

    Article Title: Structure of the human Bre1 complex bound to the nucleosome

    doi: 10.1038/s41467-024-46910-8

    Figure Lengend Snippet: a Model structure of the RINGA-RINGB-Rad6A-ubiquitin complex bound to the nucleosome in two views. b Close-up view of ubiquitin and H2B. Two lysine residues (H2BK120 and H2BK116) near G76 of ubiquitin are shown. H2BS112, whose GlcNAcylation stimulates H2BK120 ubiquitination, is also shown. c Proposed mechanistic model. The wild-type Bre1 complex can bind to the nucleosome in two orientations, but H2BK120 ubiquitination occurs only when Bre1A binds to the acidic patch, as RING A , but not RING B , can recruit Rad6A and ubiquitin. Bre1B with G974T B -A978T B double substitution can recruit Rad6A and ubiquitin; thus, H2BK120 ubiquitination occurs in both binding modes.

    Article Snippet: For Western blotting, Ubiquityl-Histone H2B (Lys120) (D11) Rabbit mAb (No. 5546; Cell Signaling) was used as the primary antibody (1:1000 dilution), goat anti-rabbit IgG-HRP (sc-2004; Santa Cruz Biotechnology) as the secondary antibody (1:2000 dilution), and ECL Prime (Cytiva) as the chemiluminescent reagent.

    Techniques: Binding Assay