anti gsc  (Thermo Fisher)


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    Name:
    GSC Monoclonal Antibody 1C2
    Description:
    GSC Monoclonal Antibody for Western Blot IHC PFA ELISA
    Catalog Number:
    MA523070
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher anti gsc
    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) <t>MYC,</t> at two positions- the minimal promoter binding site and the upstream binding site, (D) <t>GSC.</t> All error bars represent standard error within triplicates.
    GSC Monoclonal Antibody for Western Blot IHC PFA ELISA
    https://www.bioz.com/result/anti gsc/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gsc - by Bioz Stars, 2021-07
    92/100 stars

    Images

    1) Product Images from "Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines"

    Article Title: Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines

    Journal: Genes, chromosomes & cancer

    doi: 10.1002/gcc.22735

    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.
    Figure Legend Snippet: Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    Techniques Used: Binding Assay, Positive Control, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines
    Article Snippet: Chromatin was sheared by sonication for 2x [4× 20s on/20s off] by the Bioruptor Pico (Diagenode, Denville, NJ). .. The following antibodies were used (10 µg per ChIP): anti-CTCF (#2899, Cell Signaling Technology, Danvers, CA), anti-MYC (#9402, Cell Signaling Technology), anti-GABPA (#27795, ThermoFisher), and anti-GSC (#40495, ThermoFisher). .. DNA was purified by MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

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  • 92
    Thermo Fisher anti gsc
    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) <t>MYC,</t> at two positions- the minimal promoter binding site and the upstream binding site, (D) <t>GSC.</t> All error bars represent standard error within triplicates.
    Anti Gsc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gsc/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gsc - by Bioz Stars, 2021-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    Journal: Genes, chromosomes & cancer

    Article Title: Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines

    doi: 10.1002/gcc.22735

    Figure Lengend Snippet: Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    Article Snippet: The following antibodies were used (10 µg per ChIP): anti-CTCF (#2899, Cell Signaling Technology, Danvers, CA), anti-MYC (#9402, Cell Signaling Technology), anti-GABPA (#27795, ThermoFisher), and anti-GSC (#40495, ThermoFisher).

    Techniques: Binding Assay, Positive Control, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction