anti Goat IgG H L Cross Adsorbed
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1) Product Images from "Disease progression in iridocorneal angle tissues of BMP2-induced ocular hypertensive mice with optical coherence tomography"
Article Title: Disease progression in iridocorneal angle tissues of BMP2-induced ocular hypertensive mice with optical coherence tomography
Journal: Molecular Vision
Figure Legend Snippet: Assessment of damage to peripheral retinal ganglion cells and optic nerves in the mouse eyes that overexpressed BMP2 in conventional outflow tissues. Thirty-six days after the viral infusion, the mouse eyes and the optic nerves were collected. A : An image from the periphery of the flatmounted retinas of transduced mouse eyes that were immunostained with Brn3a immunoglobulin G (IgG) and imaged with epifluorescence microscopy. B : The morphology of the myelinated axons (toluidine blue stained) of mice 36 days after intracameral transduction with BMP2. The data are representative of images taken of four mice.
Techniques Used: Epifluorescence Microscopy, Staining, Mouse Assay, Transduction
2) Product Images from "The FGFR/MEK/ERK/brachyury pathway is critical for chordoma cell growth and survival"
Article Title: The FGFR/MEK/ERK/brachyury pathway is critical for chordoma cell growth and survival
Figure Legend Snippet: Expressions of FGF2 and FGFR/MEK/ERK/brachyury in chordoma cells. ( A ) Protein extracts from JHC7, UCH1 and UCH2 cells were used for western blot analysis of FGFR1, 2, 3 and 4, MEK phosphorylation (p-MEK 1/2), MEK 1/2, ERK phosphorylation (p-ERK 1/2), ERK (ERK 1/2) and brachyury. ( B ) Human foreskin fibroblast BJ cells (as a control) and chordoma cells (JHC7, UCH1 and UCH2) were cultured for 3 days in serum-free medium. FGF2 secreted into the medium was measured by ELISA and normalized to protein measured in cell lysates. ( C ) JHC7, UCH1 and UCH2 cells was incubated in serum-free medium (control) and treated with neutralizing antibody to FGF2 (FGF2 antibody, 20 µg/ml) or normal IgG (20 µg/ml) for 48h. Protein extracts from cells were used for western blot analysis of FGF2, MEK phosphorylation (p-MEK 1/2), MEK 1/2, ERK phosphorylation (p-ERK 1/2), ERK (ERK 1/2) and brachyury. ( D , E and F ) JHC7, UCH1 and UCH2 cells were incubated in triplicate serum-free medium (control) and treated with neutralizing antibody to FGF2 (FGF2 antibody, 20 µg/ml) or normal IgG (20 µg/ml) as negative control for 48h (D E) or 5 days (F). Caspase 3 activity (D), DNA fragmentation (E) and cell growth (F) were measured by the Caspase-GloR 3/7 assay, a DNA Cell Death Detection ELISA PLUS Kit and MTS assay. Values represent means ± SD ( n = 3). * P
Techniques Used: Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay, Incubation, Negative Control, Activity Assay, MTS Assay
Article Title: Transferrin Receptor Is a Specific Ferroptosis Marker
Article Snippet: Sections were washed with PBT for three times before incubating with goat anti-mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,