anti goat igg  (Nordic-Mubio)

 
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    Name:
    Goat IgG
    Description:
    Chromatographically purified Goat IgG Caprine
    Catalog Number:
    GIgG
    Price:
    [322.26]
    Size:
    100 mg
    Category:
    Antibody
    Antibody Type:
    Isotype control
    Reactivity:
    Caprids
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    Structured Review

    Nordic-Mubio anti goat igg
    Chromatographically purified Goat IgG Caprine
    https://www.bioz.com/result/anti goat igg/product/Nordic-Mubio
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti goat igg - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Staining:

    Article Title: Evidence for different origin of sex chromosomes in snakes, birds, and mammals and step-wise differentiation of snake sex chromosomes
    Article Snippet: .. For cDNA mapping, the slides were reacted with goat anti-biotin antibody (Vector Laboratories, Burlingame, CA) and then stained with fluoresceinated anti-goat IgG (Nordic Immunology, Tilburg, The Netherlands). .. FISH images were observed under a fluorescence microscope (Nikon, Tokyo, Japan) using B-2A and UV-2A filter sets.

    Incubation:

    Article Title: Removal of N-Linked Glycosylation Sites in the V1 Region of Simian Immunodeficiency Virus gp120 Results in Redirection of B-Cell Responses to V3
    Article Snippet: .. After the appropriate wash step (phosphate-buffered saline [PBS] alone for endpoint titer and conformational dependence; 8 M urea versus PBS for antibody avidity), all wells were incubated with peroxidase-labeled goat anti-monkey immunoglobulin G (IgG) or goat anti-mouse IgG (Nordic Immunology) for 1 h at room temperature. .. All wells were incubated with TM blue (Serologicals Corp., Gaithersburg, Md.) substrate for 20 min at room temperature, color was developed by the addition of 1 N sulfuric acid, and the optical density at 450 nm (OD450 ) was read using an automated ELISA plate reader (Dynex Technologies).

    Article Title: Kinetic Rates of Antibody Binding Correlate with Neutralization Sensitivity of Variant Simian Immunodeficiency Virus Strains †
    Article Snippet: .. Following extensive washing, peroxidase-conjugated anti-monkey immunoglobulin G (IgG) (Nordic Immunology Laboratories, Tilburg, The Netherlands) was diluted in blotto, added to each well, and incubated for 1 h at room temperature. .. After a final washing step, all wells were incubated with TM Blue substrate (Serologicals Corp., Norcross, GA) for 20 min at room temperature, color was developed by the addition of 1 N sulfuric acid, and the wells were read (optical density [OD] at 450 nm) using an automated ELISA plate reader (Thermo Electron Corp., Waltham, MA).

    Article Title: Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti
    Article Snippet: .. Blocking was performed with 2 % casein (Sigma) in PBS containing 0.05 % Tween20 (PBS-T) for 2 h. Wells were incubated with duck sera diluted 1:160 in PBS-T for 2 h, washed 3 × 1 min with 150 μl of PBS-T and then incubated with HRP-labelled goat anti-duck “IgG” (= anti-duck IgY, whole molecule, supplied under this “erroneous label” by KPL) diluted 1:500 in PBS-T or anti-IgM (Nordic Immunology) diluted 1:5000 in PBS-T (according to the manufacturer’s recommendations) for 1.5 h. The wells were washed 6 × 1 min with 150 μl of PBS-T and developed using tetramethylbenzidine (TMB) substrate kit (Sigma T0440). .. Reaction was stopped by addition of 1 M HCl (100 μl/well) and read at 450 nm using Infinite M200 reader (Tecan, Austria).

    other:

    Article Title: Anti-GM1 Antibodies Cause Complement-Mediated Disruption of Sodium Channel Clusters in Peripheral Motor Nerve Fibers
    Article Snippet: Fluorescein isothiocyanate-conjugated goat IgG to C3 component of rabbit complement was purchased from Nordic Immunological Laboratories (Tilburg, The Netherlands).

    Blocking Assay:

    Article Title: Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti
    Article Snippet: .. Blocking was performed with 2 % casein (Sigma) in PBS containing 0.05 % Tween20 (PBS-T) for 2 h. Wells were incubated with duck sera diluted 1:160 in PBS-T for 2 h, washed 3 × 1 min with 150 μl of PBS-T and then incubated with HRP-labelled goat anti-duck “IgG” (= anti-duck IgY, whole molecule, supplied under this “erroneous label” by KPL) diluted 1:500 in PBS-T or anti-IgM (Nordic Immunology) diluted 1:5000 in PBS-T (according to the manufacturer’s recommendations) for 1.5 h. The wells were washed 6 × 1 min with 150 μl of PBS-T and developed using tetramethylbenzidine (TMB) substrate kit (Sigma T0440). .. Reaction was stopped by addition of 1 M HCl (100 μl/well) and read at 450 nm using Infinite M200 reader (Tecan, Austria).

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  • 88
    Nordic-Mubio goat anti rhesus igg
    Effect of administration of rUre plus LT or placebo plus LT on conversion in specific antiurease serum and saliva <t>IgG</t> and <t>IgA</t> at 1 week after the fourth immunization. The H. pylori + or − designation reflects the status at endoscopy 10 months after the first immunization. ODU, OD units.
    Goat Anti Rhesus Igg, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rhesus igg/product/Nordic-Mubio
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rhesus igg - by Bioz Stars, 2020-09
    88/100 stars
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    85
    Nordic-Mubio igg2b
    Immunofluorescent detection of glomerular deposits of <t>IgG1</t> in the kidneys of DBA/2 mice administered mercuric chloride in combination with lipopolysaccharide (LPS). The kidneys from the same animals presented in were tested for the presence of
    Igg2b, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2b/product/Nordic-Mubio
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    85
    Nordic-Mubio goat anti mouse igg1
    Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and <t>IgG1</t> antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P
    Goat Anti Mouse Igg1, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg1/product/Nordic-Mubio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg1 - by Bioz Stars, 2020-09
    85/100 stars
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    90
    Nordic-Mubio peroxidase conjugated goat anti human igg antibody
    Improvement of soluble antigen export from endosomes into cytosol in chloroquine-treated iDCs. (A and B) iDCs were pulsed/chased with either NS3Ag alone or NS3Ag and TRITC-Tf, in the presence or absence of chloroquine, fixed, and stained at different times with human anti-NS3 alone or with anti-NS3 + rabbit anti–cathepsin D (C and D), followed by staining with the appropriate secondary antibodies. The signal of NS3 alone is green. The signals of double staining of Tf or CD and NS3 are red and green, respectively. The extent of colocalization is shown in yellow after merging. Arrows indicate NS3 enrichment or colocalization with Tf or CD. In control experiments, DCs were incubated for 1 h at 37°C in the presence of 1 mg/ml FITC-dextran with the indicated molecular masses and then fixed. One representative out of three different experiments is shown. (C) Quantitative analysis of the fluorescence intensity of cytosolic NS3Ag in iDCs pulsed/chased with NS3Ag in the presence or absence of chloroquine. (D) Quantitative analysis of the fluorescence intensity of cytosolic FITC-dextran in iDCs pulsed/chased with FITC-dextran of the indicated molecular masses, in the presence or absence of chloroquine. (E) One representative out of three WB analyses on both endocytic- and cytosol-enriched preparations, purified from iDCs, which had been previously pulsed/chased (20′/90′) with the indicated concentrations of NS3Ag, in the presence or absence of chloroquine. The two preparations were then separated by SDS-PAGE and immunoblotted for NS3Ag with the relevant human mAb and a secondary peroxidase-conjugated goat <t>anti–human</t> <t>IgG</t> antibody. NS3Ag alone (↓) was used as positive control. (F) Fluorometric analyses of different FITC-dextrans in endocytic or cytosolic fractions from iDCs pulsed with the indicated FITC-dextrans in the presence or absence of chloroquine. (G) One representative kinetic out of four detected by FC of NS3Ag, TAP-2, or LAMP-2 staining in phagosomes containing a mixture of soluble NS3Ag and LB. Analyses were performed on gated phagosomes containing LB. Isotype control, green; staining after 20′ of pulsing, red; staining after 20' of pulsing + 90′ of chase, blue.
    Peroxidase Conjugated Goat Anti Human Igg Antibody, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti human igg antibody/product/Nordic-Mubio
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Effect of administration of rUre plus LT or placebo plus LT on conversion in specific antiurease serum and saliva IgG and IgA at 1 week after the fourth immunization. The H. pylori + or − designation reflects the status at endoscopy 10 months after the first immunization. ODU, OD units.

    Journal: Infection and Immunity

    Article Title: Immunization against Natural Helicobacter pylori Infection in Nonhuman Primates

    doi:

    Figure Lengend Snippet: Effect of administration of rUre plus LT or placebo plus LT on conversion in specific antiurease serum and saliva IgG and IgA at 1 week after the fourth immunization. The H. pylori + or − designation reflects the status at endoscopy 10 months after the first immunization. ODU, OD units.

    Article Snippet: The detecting antibodies were goat anti-rhesus IgG or IgA conjugated to horseradish peroxidase (Nordic Immunological Laboratories, The Netherlands).

    Techniques:

    Immunofluorescent detection of glomerular deposits of IgG1 in the kidneys of DBA/2 mice administered mercuric chloride in combination with lipopolysaccharide (LPS). The kidneys from the same animals presented in were tested for the presence of

    Journal:

    Article Title: Bacterial lipopolysaccharide both renders resistant mice susceptible to mercury-induced autoimmunity and exacerbates such autoimmunity in susceptible mice

    doi: 10.1111/j.1365-2249.2005.02849.x

    Figure Lengend Snippet: Immunofluorescent detection of glomerular deposits of IgG1 in the kidneys of DBA/2 mice administered mercuric chloride in combination with lipopolysaccharide (LPS). The kidneys from the same animals presented in were tested for the presence of

    Article Snippet: [ ], employing rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC, USA) and IgG2b (Nordic Immunological Laboratories, Tillburg, the Netherlands) as the developing reagents.

    Techniques: Mouse Assay

    Administration of bacterial lipopolysaccharide (LPS) together with mercuric chloride to DBA/2 mice increases their production of IgG1 antibodies to both exogenous and endogenous antigens. The levels of IgG1 antibodies directed against TNP (a), ssDNA (b),

    Journal:

    Article Title: Bacterial lipopolysaccharide both renders resistant mice susceptible to mercury-induced autoimmunity and exacerbates such autoimmunity in susceptible mice

    doi: 10.1111/j.1365-2249.2005.02849.x

    Figure Lengend Snippet: Administration of bacterial lipopolysaccharide (LPS) together with mercuric chloride to DBA/2 mice increases their production of IgG1 antibodies to both exogenous and endogenous antigens. The levels of IgG1 antibodies directed against TNP (a), ssDNA (b),

    Article Snippet: [ ], employing rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC, USA) and IgG2b (Nordic Immunological Laboratories, Tillburg, the Netherlands) as the developing reagents.

    Techniques: Mouse Assay

    Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and IgG1 antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P

    Journal: Infection and Immunity

    Article Title: Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling

    doi: 10.1128/IAI.69.3.1714-1721.2001

    Figure Lengend Snippet: Induction of specific immune responses in IL-4Rα −/− mice receiving UreA-specific CD4 + T cells. (A) Proliferation of splenic CD4 + T cells isolated from recipient challenged or nonrecipient infected mice. Results are expressed as mean ± standard error of the mean. (B) Analysis of IgG2a and IgG1 antibody responses in serum. Results are expressed as the mean ± standard deviation. IgG2a titers in recipient mice were significantly higher than in nonrecipient mice (∗) ( P

    Article Snippet: Bound specific antibodies were detected with goat anti-mouse IgG2a or goat anti-mouse IgG1 conjugated to horseradish peroxidase, used at a dilution of 1/5,000 (Nordic Immunological Laboratories, Tilburg, The Netherlands).

    Techniques: Mouse Assay, Isolation, Infection, Standard Deviation

    Mice which received UreA-specific CD4 + T cells mounted IgG2a and IgG1 antibody responses against urease. Sera were taken 6 weeks after infection. Results are expressed as mean ± standard deviation. Groups contained five to seven mice. Statistically significant differences (two-tailed t test) between recipient and nonrecipient mice were calculated for IgG2a ( P > 0.1) and IgG1 ( P

    Journal: Infection and Immunity

    Article Title: Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling

    doi: 10.1128/IAI.69.3.1714-1721.2001

    Figure Lengend Snippet: Mice which received UreA-specific CD4 + T cells mounted IgG2a and IgG1 antibody responses against urease. Sera were taken 6 weeks after infection. Results are expressed as mean ± standard deviation. Groups contained five to seven mice. Statistically significant differences (two-tailed t test) between recipient and nonrecipient mice were calculated for IgG2a ( P > 0.1) and IgG1 ( P

    Article Snippet: Bound specific antibodies were detected with goat anti-mouse IgG2a or goat anti-mouse IgG1 conjugated to horseradish peroxidase, used at a dilution of 1/5,000 (Nordic Immunological Laboratories, Tilburg, The Netherlands).

    Techniques: Mouse Assay, Infection, Standard Deviation, Two Tailed Test

    Improvement of soluble antigen export from endosomes into cytosol in chloroquine-treated iDCs. (A and B) iDCs were pulsed/chased with either NS3Ag alone or NS3Ag and TRITC-Tf, in the presence or absence of chloroquine, fixed, and stained at different times with human anti-NS3 alone or with anti-NS3 + rabbit anti–cathepsin D (C and D), followed by staining with the appropriate secondary antibodies. The signal of NS3 alone is green. The signals of double staining of Tf or CD and NS3 are red and green, respectively. The extent of colocalization is shown in yellow after merging. Arrows indicate NS3 enrichment or colocalization with Tf or CD. In control experiments, DCs were incubated for 1 h at 37°C in the presence of 1 mg/ml FITC-dextran with the indicated molecular masses and then fixed. One representative out of three different experiments is shown. (C) Quantitative analysis of the fluorescence intensity of cytosolic NS3Ag in iDCs pulsed/chased with NS3Ag in the presence or absence of chloroquine. (D) Quantitative analysis of the fluorescence intensity of cytosolic FITC-dextran in iDCs pulsed/chased with FITC-dextran of the indicated molecular masses, in the presence or absence of chloroquine. (E) One representative out of three WB analyses on both endocytic- and cytosol-enriched preparations, purified from iDCs, which had been previously pulsed/chased (20′/90′) with the indicated concentrations of NS3Ag, in the presence or absence of chloroquine. The two preparations were then separated by SDS-PAGE and immunoblotted for NS3Ag with the relevant human mAb and a secondary peroxidase-conjugated goat anti–human IgG antibody. NS3Ag alone (↓) was used as positive control. (F) Fluorometric analyses of different FITC-dextrans in endocytic or cytosolic fractions from iDCs pulsed with the indicated FITC-dextrans in the presence or absence of chloroquine. (G) One representative kinetic out of four detected by FC of NS3Ag, TAP-2, or LAMP-2 staining in phagosomes containing a mixture of soluble NS3Ag and LB. Analyses were performed on gated phagosomes containing LB. Isotype control, green; staining after 20′ of pulsing, red; staining after 20' of pulsing + 90′ of chase, blue.

    Journal: The Journal of Experimental Medicine

    Article Title: Chloroquine enhances human CD8+ T cell responses against soluble antigens in vivo

    doi: 10.1084/jem.20051106

    Figure Lengend Snippet: Improvement of soluble antigen export from endosomes into cytosol in chloroquine-treated iDCs. (A and B) iDCs were pulsed/chased with either NS3Ag alone or NS3Ag and TRITC-Tf, in the presence or absence of chloroquine, fixed, and stained at different times with human anti-NS3 alone or with anti-NS3 + rabbit anti–cathepsin D (C and D), followed by staining with the appropriate secondary antibodies. The signal of NS3 alone is green. The signals of double staining of Tf or CD and NS3 are red and green, respectively. The extent of colocalization is shown in yellow after merging. Arrows indicate NS3 enrichment or colocalization with Tf or CD. In control experiments, DCs were incubated for 1 h at 37°C in the presence of 1 mg/ml FITC-dextran with the indicated molecular masses and then fixed. One representative out of three different experiments is shown. (C) Quantitative analysis of the fluorescence intensity of cytosolic NS3Ag in iDCs pulsed/chased with NS3Ag in the presence or absence of chloroquine. (D) Quantitative analysis of the fluorescence intensity of cytosolic FITC-dextran in iDCs pulsed/chased with FITC-dextran of the indicated molecular masses, in the presence or absence of chloroquine. (E) One representative out of three WB analyses on both endocytic- and cytosol-enriched preparations, purified from iDCs, which had been previously pulsed/chased (20′/90′) with the indicated concentrations of NS3Ag, in the presence or absence of chloroquine. The two preparations were then separated by SDS-PAGE and immunoblotted for NS3Ag with the relevant human mAb and a secondary peroxidase-conjugated goat anti–human IgG antibody. NS3Ag alone (↓) was used as positive control. (F) Fluorometric analyses of different FITC-dextrans in endocytic or cytosolic fractions from iDCs pulsed with the indicated FITC-dextrans in the presence or absence of chloroquine. (G) One representative kinetic out of four detected by FC of NS3Ag, TAP-2, or LAMP-2 staining in phagosomes containing a mixture of soluble NS3Ag and LB. Analyses were performed on gated phagosomes containing LB. Isotype control, green; staining after 20′ of pulsing, red; staining after 20' of pulsing + 90′ of chase, blue.

    Article Snippet: The cytosolic- and endocytic-enriched fractions, obtained as described above, were separated by SDS-PAGE and immunoblotted for NS3Ag with the relevant human mAb and the secondary peroxidase-conjugated goat anti–human IgG antibody (Nordic Immunology).

    Techniques: Staining, Double Staining, Incubation, Fluorescence, Western Blot, Purification, SDS Page, Positive Control