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Santa Cruz Biotechnology anti goat igg secondary abs
Anti Goat Igg Secondary Abs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti goat igg secondary abs/product/Santa Cruz Biotechnology
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
anti goat igg secondary abs - by Bioz Stars, 2020-09
85/100 stars

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Western Blot:

Article Title: Arsenic-Specific Stem Cell Selection During Malignant Transformation
Article Snippet: .. The membranes were washed and incubated with horseradish peroxidase–conjugated anti-mouse, anti-rabbit, or anti-goat IgG secondary antibodies (1:1000 to 1:2000 dilution; Santa Cruz Biotechnology) as appropriate, and bound antibody was detected using Amersham ECL Western Blot Detection Reagents (GE Healthcare, Buckinghamshire, UK). .. Membranes were then stripped with Restore Western Blot Stripping Buffer (Pierce) and incubated with anti-β-actin antibody (JLA20, mouse monoclonal, 1:2500 dilution; Calbiochem) followed by incubation with horseradish peroxidase–conjugated anti-mouse IgG (Santa Cruz Biotechnology).

Incubation:

Article Title: Arsenic-Specific Stem Cell Selection During Malignant Transformation
Article Snippet: .. The membranes were washed and incubated with horseradish peroxidase–conjugated anti-mouse, anti-rabbit, or anti-goat IgG secondary antibodies (1:1000 to 1:2000 dilution; Santa Cruz Biotechnology) as appropriate, and bound antibody was detected using Amersham ECL Western Blot Detection Reagents (GE Healthcare, Buckinghamshire, UK). .. Membranes were then stripped with Restore Western Blot Stripping Buffer (Pierce) and incubated with anti-β-actin antibody (JLA20, mouse monoclonal, 1:2500 dilution; Calbiochem) followed by incubation with horseradish peroxidase–conjugated anti-mouse IgG (Santa Cruz Biotechnology).

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  • 91
    Santa Cruz Biotechnology tritc conjugated secondary antibody
    Immunofluorescent images showing the deposition of osteocalcin (green, FITC) and osteopontin (red, <t>TRITC)</t> on nanofiber scaffold surfaces. Images obtained on 0% HAp ( A B ), 1% HAp ( C D ), or 10% HAp ( E F ) scaffolds. Magnification is 10×, scale bar is 50 mm in all images.
    Tritc Conjugated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tritc conjugated secondary antibody/product/Santa Cruz Biotechnology
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    tritc conjugated secondary antibody - by Bioz Stars, 2020-09
    91/100 stars
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    93
    Santa Cruz Biotechnology fitc labeled secondary antibody
    Immunofluorescence staining assayed the p11 protein in SH-sy5y cells treated with hIFN-α-2b (0, 500, and 3000 IU/mL) for 24 h. The cells were stained with antibodies against p11 (primary antibody) and <t>FITC-labeled</t> secondary antibodies. ( A ) The FITC column in the micrographs showed the p11 protein levels. The white numbers at the bottom left of each section represented the average fluorescent intensity (MFI). In the middle column, DAPI staining shows the cells nuclei. Merged images of FITC and DAPI staining were shown in the column on the right. Confocal microscopy was conducted at 400× and the scale bar represents 20 μm. The negative control group was stained with rabbit <t>IgG</t> instead of the primer antibody. ( B ) Histogram of the mean fluorescence intensity of FITC. All results were representative of three separate experiments. The data represented the mean ± S.E. compared with the controls. * P
    Fitc Labeled Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc labeled secondary antibody/product/Santa Cruz Biotechnology
    Average 93 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    fitc labeled secondary antibody - by Bioz Stars, 2020-09
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    90
    Santa Cruz Biotechnology alexa fluor 594 conjugate goat anti rabbit igg
    Labeling of nuclear envelope proteins SUN2, Nesprin2, and Emerin in H. capsulatum infected alveolar macrophages . In Panels (A–C) show confocal scanning microscopy images of representative AMJ2-C11 macrophages infected with H. capsulatum strains EH-315 and 60I at 37°C for 5 h. Non-infected macrophages were processed as a control. (A) AMJ2-C11 stained for SUN2, (B) Nesprin2, and (C) Emerin as indicated in the figure. The arrows show phagosome formation where several yeast cells are present. DAPI was used for nuclear staining (blue). <t>Alexa</t> Fluor®488 and Alexa <t>Fluor®594</t> conjugates were used as secondary antibodies to reveal nuclear proteins (green) and yeast cells (red), respectively. Bright field images were merged with Alexa Fluor®594 and DAPI stain with orthogonal z stack sections after 5 h of macrophage-yeast infection. The assay was performed twice.
    Alexa Fluor 594 Conjugate Goat Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 594 conjugate goat anti rabbit igg/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 conjugate goat anti rabbit igg - by Bioz Stars, 2020-09
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    88
    Santa Cruz Biotechnology secondary antibody goat anti mouse igg fitc
    rADAM9D binds to DU145 through β1, α6, αvβ5 and αvβ3 integrins as demonstrated by an antibody competition assay ( A ) and by flow cytometry analysis ( B ). The integrin content of DU145 cell line was assessed by flow cytometry ( C ). ( A ) For antibody competition assay CMFDA-labeled cells were incubated with different anti-integrin antibodies (β1, α6, αvβ5, αvβ3, α2 and α4, at 10 µg/ml) and <t>IgG</t> control (10 µg/ml) before being plated on rADAM9D-coated (10 µg) wells. DU145 directly plated on rADAM9D or IgG-coated wells, without previous incubation with any antibody was used as positive control and BSA was used as negative control. ( B ) The integrin content of DU145 cells was determined by flow cytometry. Cells (1 × 10 5 ) were incubated for 40 min at 4°C with the specific antibodies cited earlier or control IgG. Cells were washed and incubated with secondary antibody labeled with <t>FITC,</t> at same conditions described before, washed and fixed with FACs buffer contaning 1% phormaldehyde overnight at 4°C. C. To verify the interaction with integrins, rADAM9D (1 µM) was previously incubated (30 min at room temperature) with DU145 cells, before the addition of antibodies. Cells were analyzed in FACSCanto. The results were obtained from 3 independent experiments in triplicate. The error bars show the SE of three samples with less deviation from the mean. The means that are significantly different from rADAM9D and IgG-coated wells using ANOVA followed of post hoc Dunnett's test were shown by *( P ≤ 0.001).
    Secondary Antibody Goat Anti Mouse Igg Fitc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibody goat anti mouse igg fitc/product/Santa Cruz Biotechnology
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary antibody goat anti mouse igg fitc - by Bioz Stars, 2020-09
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    Image Search Results


    Immunofluorescent images showing the deposition of osteocalcin (green, FITC) and osteopontin (red, TRITC) on nanofiber scaffold surfaces. Images obtained on 0% HAp ( A B ), 1% HAp ( C D ), or 10% HAp ( E F ) scaffolds. Magnification is 10×, scale bar is 50 mm in all images.

    Journal: Journal of Functional Biomaterials

    Article Title: Mineralization Content Alters Osteogenic Responses of Bone Marrow Stromal Cells on Hydroxyapatite/Polycaprolactone Composite Nanofiber Scaffolds

    doi: 10.3390/jfb3040776

    Figure Lengend Snippet: Immunofluorescent images showing the deposition of osteocalcin (green, FITC) and osteopontin (red, TRITC) on nanofiber scaffold surfaces. Images obtained on 0% HAp ( A B ), 1% HAp ( C D ), or 10% HAp ( E F ) scaffolds. Magnification is 10×, scale bar is 50 mm in all images.

    Article Snippet: After an additional blocking step and PBS wash, the scaffolds were then incubated in a FITC-conjugated or TRITC-conjugated secondary antibody (1:200 donkey anti-goat IgG, Santa Cruz Biotechnology) for 45 min in the dark.

    Techniques:

    Immunofluorescence staining assayed the p11 protein in SH-sy5y cells treated with hIFN-α-2b (0, 500, and 3000 IU/mL) for 24 h. The cells were stained with antibodies against p11 (primary antibody) and FITC-labeled secondary antibodies. ( A ) The FITC column in the micrographs showed the p11 protein levels. The white numbers at the bottom left of each section represented the average fluorescent intensity (MFI). In the middle column, DAPI staining shows the cells nuclei. Merged images of FITC and DAPI staining were shown in the column on the right. Confocal microscopy was conducted at 400× and the scale bar represents 20 μm. The negative control group was stained with rabbit IgG instead of the primer antibody. ( B ) Histogram of the mean fluorescence intensity of FITC. All results were representative of three separate experiments. The data represented the mean ± S.E. compared with the controls. * P

    Journal: Scientific Reports

    Article Title: Probable involvement of p11 with interferon alpha induced depression

    doi: 10.1038/srep17029

    Figure Lengend Snippet: Immunofluorescence staining assayed the p11 protein in SH-sy5y cells treated with hIFN-α-2b (0, 500, and 3000 IU/mL) for 24 h. The cells were stained with antibodies against p11 (primary antibody) and FITC-labeled secondary antibodies. ( A ) The FITC column in the micrographs showed the p11 protein levels. The white numbers at the bottom left of each section represented the average fluorescent intensity (MFI). In the middle column, DAPI staining shows the cells nuclei. Merged images of FITC and DAPI staining were shown in the column on the right. Confocal microscopy was conducted at 400× and the scale bar represents 20 μm. The negative control group was stained with rabbit IgG instead of the primer antibody. ( B ) Histogram of the mean fluorescence intensity of FITC. All results were representative of three separate experiments. The data represented the mean ± S.E. compared with the controls. * P

    Article Snippet: After five washes with TBS-T, the sections were incubated with an FITC-labeled secondary antibody (goat anti-rabbit IgG-FITC 1:2000, Santa Cruz) for 1 h at room temperature, followed by three washes with TBS-T. Next, the cells were stained with DAPI (4,6-diamidino-2-phenylindole, Invitrogen) for 20 min and sealed with 20 μL antifade mounting medium.

    Techniques: Immunofluorescence, Staining, Labeling, Confocal Microscopy, Negative Control, Fluorescence

    Labeling of nuclear envelope proteins SUN2, Nesprin2, and Emerin in H. capsulatum infected alveolar macrophages . In Panels (A–C) show confocal scanning microscopy images of representative AMJ2-C11 macrophages infected with H. capsulatum strains EH-315 and 60I at 37°C for 5 h. Non-infected macrophages were processed as a control. (A) AMJ2-C11 stained for SUN2, (B) Nesprin2, and (C) Emerin as indicated in the figure. The arrows show phagosome formation where several yeast cells are present. DAPI was used for nuclear staining (blue). Alexa Fluor®488 and Alexa Fluor®594 conjugates were used as secondary antibodies to reveal nuclear proteins (green) and yeast cells (red), respectively. Bright field images were merged with Alexa Fluor®594 and DAPI stain with orthogonal z stack sections after 5 h of macrophage-yeast infection. The assay was performed twice.

    Journal: Frontiers in Microbiology

    Article Title: An Intracellular Arrangement of Histoplasma capsulatum Yeast-Aggregates Generates Nuclear Damage to the Cultured Murine Alveolar Macrophages

    doi: 10.3389/fmicb.2015.01526

    Figure Lengend Snippet: Labeling of nuclear envelope proteins SUN2, Nesprin2, and Emerin in H. capsulatum infected alveolar macrophages . In Panels (A–C) show confocal scanning microscopy images of representative AMJ2-C11 macrophages infected with H. capsulatum strains EH-315 and 60I at 37°C for 5 h. Non-infected macrophages were processed as a control. (A) AMJ2-C11 stained for SUN2, (B) Nesprin2, and (C) Emerin as indicated in the figure. The arrows show phagosome formation where several yeast cells are present. DAPI was used for nuclear staining (blue). Alexa Fluor®488 and Alexa Fluor®594 conjugates were used as secondary antibodies to reveal nuclear proteins (green) and yeast cells (red), respectively. Bright field images were merged with Alexa Fluor®594 and DAPI stain with orthogonal z stack sections after 5 h of macrophage-yeast infection. The assay was performed twice.

    Article Snippet: Primary anti-H. capsulatum antibody was added for 1 h. Unbound antibodies were removed by washing in PBS, then, Alexa Fluor®594-conjugate goat anti-rabbit IgG (secondary antibody) was added for 1 h. Afterward, in another staining series, anti-SUN2 antibody (Santa Cruz Biotechnology Inc., Heidelberg, Germany), anti-Nesprin2 antibody (Abcam, Cambridge, UK), or anti-Emerin antibody (Abcam) obtained in mice was added as a primary antibody, and macrophage samples were incubated overnight.

    Techniques: Labeling, Infection, Microscopy, Staining

    Effect of H. capsulatum yeast cells on the infection of AMJ2-C11 macrophages . Macrophages infected with H. capsulatum yeast cells were incubated at 37°C for 5 h (see details in Section Materials and Methods). (A,B) AMJ2-C11 macrophages infected with the EH-315; (C) AMJ2-C11 macrophages infected with 60I; and (D) non-infected AMJ2-C11 macrophages. Indirect immunofluorescence: FITC-phalloidin in green showing the macrophage cytoplasm; Alexa Fluor®594 in red to yellow staining H. capsulatum yeast cells; DAPI in blue staining macrophages nucleus. Images were obtained using IN Cell Analyzer light microscopy. The results are representative of two assays. Arrows indicate the architectural arrangement of yeast-aggregates apparently surrounding the nuclei of the phagocytic cells.

    Journal: Frontiers in Microbiology

    Article Title: An Intracellular Arrangement of Histoplasma capsulatum Yeast-Aggregates Generates Nuclear Damage to the Cultured Murine Alveolar Macrophages

    doi: 10.3389/fmicb.2015.01526

    Figure Lengend Snippet: Effect of H. capsulatum yeast cells on the infection of AMJ2-C11 macrophages . Macrophages infected with H. capsulatum yeast cells were incubated at 37°C for 5 h (see details in Section Materials and Methods). (A,B) AMJ2-C11 macrophages infected with the EH-315; (C) AMJ2-C11 macrophages infected with 60I; and (D) non-infected AMJ2-C11 macrophages. Indirect immunofluorescence: FITC-phalloidin in green showing the macrophage cytoplasm; Alexa Fluor®594 in red to yellow staining H. capsulatum yeast cells; DAPI in blue staining macrophages nucleus. Images were obtained using IN Cell Analyzer light microscopy. The results are representative of two assays. Arrows indicate the architectural arrangement of yeast-aggregates apparently surrounding the nuclei of the phagocytic cells.

    Article Snippet: Primary anti-H. capsulatum antibody was added for 1 h. Unbound antibodies were removed by washing in PBS, then, Alexa Fluor®594-conjugate goat anti-rabbit IgG (secondary antibody) was added for 1 h. Afterward, in another staining series, anti-SUN2 antibody (Santa Cruz Biotechnology Inc., Heidelberg, Germany), anti-Nesprin2 antibody (Abcam, Cambridge, UK), or anti-Emerin antibody (Abcam) obtained in mice was added as a primary antibody, and macrophage samples were incubated overnight.

    Techniques: Infection, Incubation, Immunofluorescence, Staining, Light Microscopy

    rADAM9D binds to DU145 through β1, α6, αvβ5 and αvβ3 integrins as demonstrated by an antibody competition assay ( A ) and by flow cytometry analysis ( B ). The integrin content of DU145 cell line was assessed by flow cytometry ( C ). ( A ) For antibody competition assay CMFDA-labeled cells were incubated with different anti-integrin antibodies (β1, α6, αvβ5, αvβ3, α2 and α4, at 10 µg/ml) and IgG control (10 µg/ml) before being plated on rADAM9D-coated (10 µg) wells. DU145 directly plated on rADAM9D or IgG-coated wells, without previous incubation with any antibody was used as positive control and BSA was used as negative control. ( B ) The integrin content of DU145 cells was determined by flow cytometry. Cells (1 × 10 5 ) were incubated for 40 min at 4°C with the specific antibodies cited earlier or control IgG. Cells were washed and incubated with secondary antibody labeled with FITC, at same conditions described before, washed and fixed with FACs buffer contaning 1% phormaldehyde overnight at 4°C. C. To verify the interaction with integrins, rADAM9D (1 µM) was previously incubated (30 min at room temperature) with DU145 cells, before the addition of antibodies. Cells were analyzed in FACSCanto. The results were obtained from 3 independent experiments in triplicate. The error bars show the SE of three samples with less deviation from the mean. The means that are significantly different from rADAM9D and IgG-coated wells using ANOVA followed of post hoc Dunnett's test were shown by *( P ≤ 0.001).

    Journal: Cell Adhesion & Migration

    Article Title: Recombinant disintegrin domain of human ADAM9 inhibits migration and invasion of DU145 prostate tumor cells

    doi: 10.4161/19336918.2014.994917

    Figure Lengend Snippet: rADAM9D binds to DU145 through β1, α6, αvβ5 and αvβ3 integrins as demonstrated by an antibody competition assay ( A ) and by flow cytometry analysis ( B ). The integrin content of DU145 cell line was assessed by flow cytometry ( C ). ( A ) For antibody competition assay CMFDA-labeled cells were incubated with different anti-integrin antibodies (β1, α6, αvβ5, αvβ3, α2 and α4, at 10 µg/ml) and IgG control (10 µg/ml) before being plated on rADAM9D-coated (10 µg) wells. DU145 directly plated on rADAM9D or IgG-coated wells, without previous incubation with any antibody was used as positive control and BSA was used as negative control. ( B ) The integrin content of DU145 cells was determined by flow cytometry. Cells (1 × 10 5 ) were incubated for 40 min at 4°C with the specific antibodies cited earlier or control IgG. Cells were washed and incubated with secondary antibody labeled with FITC, at same conditions described before, washed and fixed with FACs buffer contaning 1% phormaldehyde overnight at 4°C. C. To verify the interaction with integrins, rADAM9D (1 µM) was previously incubated (30 min at room temperature) with DU145 cells, before the addition of antibodies. Cells were analyzed in FACSCanto. The results were obtained from 3 independent experiments in triplicate. The error bars show the SE of three samples with less deviation from the mean. The means that are significantly different from rADAM9D and IgG-coated wells using ANOVA followed of post hoc Dunnett's test were shown by *( P ≤ 0.001).

    Article Snippet: Secondary antibody goat anti-mouse IgG-FITC (Fluorescein isothiocyanate, sc-2010), used for flow cytometry, were purchased from Santa Cruz Biotechnology.

    Techniques: Competitive Binding Assay, Flow Cytometry, Cytometry, Labeling, Incubation, Positive Control, Negative Control, FACS