Journal: Spine

Article Title: Hsa_circ_0001946 Ameliorates Mechanical Stress-induced Intervertebral Disk Degeneration Via Targeting miR-432-5p and SOX9

doi: 10.1097/BRS.0000000000004777

Figure Lengend Snippet: Effects of circ-CDR1as on compression-induced NP cell apoptosis and extracellular matrix degradation. A and B, The quantitative real-time polymerase chain reaction analysis was used to determine the expression level of circ-CDR1as in human NP cells treated with circ-CDR1as siRNA (si) or circ-CDR1as OE vector. C, The western blot analysis was applied to detect the protein levels of aggrecan, collagen II, Bax, and Bcl-2 in human NP cells transfected with circ-CDR1as siRNA under compression treatment. GAPDH served as an internal control. D, The protein levels of aggrecan, collagen II, Bax, and Bcl-2 in human NP cells transfected with circ-CDR1as OE vector under compression treatment. GAPDH served as an internal control. Data were shown as means with error bars representing SD. * P <0.05, ** P <0.01, *** P <0.001, n=3. circRNA indicates circular RNA, GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NP, nucleus pulposus; OE, overexpression.

Article Snippet: Primary antibodies against the following proteins were used: glyceraldehyde 3-phosphate dehydrogenase (GAPDH, #5174; CST), Bax (ab32503; Abcam), Bcl-2 (ab196495; Abcam), collagen II (ab34712; Abcam), aggrecan (13880-1-AP; Proteintech), MMP13 (ab39012; Abcam), SOX9 (ab185966; Abcam).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Plasmid Preparation, Western Blot, Transfection, Over Expression

Journal: Spine

Article Title: Hsa_circ_0001946 Ameliorates Mechanical Stress-induced Intervertebral Disk Degeneration Via Targeting miR-432-5p and SOX9

doi: 10.1097/BRS.0000000000004777

Figure Lengend Snippet: MiR-432-5p facilitated compression-induced NP cell apoptosis and extracellular matrix degradation. A and B, The human NP cells were treated with miR-432-5p inhibitor or miR-432-5p mimics, and the relative expression levels of miR-432-5p were validated by quantitative real-time polymerase chain reaction analysis. C and D, The protein levels of aggrecan, collagen II, Bax, and Bcl-2 in human NP cells treated with miR-432-5p inhibitor or miR-432-5p mimics under compression. GAPDH served as an internal control. Data were presented as means with error bars representing SD. ** P <0.01, *** P <0.001, n=3. GAPDH indicates glyceraldehyde 3-phosphate dehydrogenase; NP, nucleus pulposus.

Article Snippet: Primary antibodies against the following proteins were used: glyceraldehyde 3-phosphate dehydrogenase (GAPDH, #5174; CST), Bax (ab32503; Abcam), Bcl-2 (ab196495; Abcam), collagen II (ab34712; Abcam), aggrecan (13880-1-AP; Proteintech), MMP13 (ab39012; Abcam), SOX9 (ab185966; Abcam).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Neural Regeneration Research

Article Title: Cyclo (MQCNS) has the potential to treat ischemic stroke

doi: 10.4103/1673-5374.371367

Figure Lengend Snippet: LZ-3 inhibits ischemia- or hypoxia-induced JAK1-STAT6 signaling. (A) Representative western blot showing p-JAK1 and p-STAT6 expression in the cortex on day 3 after reperfusion. (B, C) Quantification of p-JAK1 and p-STAT6 expression based on western blotting ( n = 3/group). ### P < 0.001, vs . sham group; ** P < 0.01, *** P < 0.001, vs . model group. (D) Representative western blot showing p-JAK1 and p-STAT6 expression in BV2 cells treated with LZ-3 or upadacitinib after 24 hours of re-oxygenation after OGD. (E, F) Quantification of p-JAK1 and p-STAT6 expression based on western blotting ( n = 3). (G) Representative western blot showing p-JAK1 and p-STAT6 expression in BV2 cells overexpressing JAK1 and treated with LZ-3 or upadacitinib after 24 hours of re-oxygenation after OGD. (H, I) Quantification of p-JAK1 and p-STAT6 expression based on western blotting ( n = 3). ### P < 0.001, vs . control group; *** P < 0.001, vs . OGD group. Data are expressed as mean ± SD and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. Con: Control group; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; JAK1: Janus kinase 1; Mod: model group; OGD: oxygen-glucose deprivation; pEX-3-JAK1: peroxisomal biogenesis factor 3-Janus kinase 1; p-JAK1: phospho-Janus kinase 1; p-STAT6: phospho-signal transducer and activator of transcription; STAT6: signal transducer and activator of transcription.

Article Snippet: Rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (1:1000, Cell Signaling Technology, Cat# 5174, RRID: AB_10622025) was used to confirm equal loading.

Techniques: Western Blot, Expressing