anti glyceraldehyde 3 phosphate dehydrogenase gapdh polyclonal  (Millipore)


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    Structured Review

    Millipore anti glyceraldehyde 3 phosphate dehydrogenase gapdh polyclonal
    Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Polyclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glyceraldehyde 3 phosphate dehydrogenase gapdh polyclonal/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glyceraldehyde 3 phosphate dehydrogenase gapdh polyclonal - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: For western blot analysis, 100, 50, or 25 μg of total proteins were separated by 8% SDS-polyacrylamide gel electrophoresis and then transferred onto 0.45 μm supported nitrocellulose membrane (Bio-Rad) at 350 mA for 90 min using a Mini Trans-Blot Cell apparatus (Bio-Rad). .. The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively.

    Protein Concentration:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: Protein concentration was determinated by the bicinchoninic acid method (BCA Protein Assay Kit, Pierce). .. The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively.

    Immunodetection:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively. .. Immunoreactivity was detected using enhanced chemiluminescence (ECL Plus, Amersham) and the WesternBreeze Chemiluminescent Western Blot Immunodetection Kit (Invitrogen).

    Incubation:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: Filters were blocked overnight at 4°C with 5% nonfat dry milk in Tris-buffered saline (TBS) and Tween 20 (19.9 mM Tris base, 136 mM NaCl, 0.1% Tween 20, pH 7.6) and incubated overnight at 4°C with the following primary antibodies: anti-Notch1 rabbit polyclonal (Upstate Biotech), anti-Notch2 goat polyclonal (Santa Cruz Biotechnology), anti-Notch3 goat polyclonal (Upstate Biotech), and anti-Notch4 rabbit polyclonal (Santa Cruz Biotechnology) diluted at 1:2000, 1:400, 1:200, and 1:200, respectively. .. The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively.

    BIA-KA:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: Protein concentration was determinated by the bicinchoninic acid method (BCA Protein Assay Kit, Pierce). .. The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively.

    Western Blot:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: Paragraph title: Western Blot Analysis ... The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively.

    Lysis:

    Article Title: Differential patterns of NOTCH1–4 receptor expression are markers of glioma cell differentiation
    Article Snippet: Cells were lysed using a lysis buffer containing Tris-HCl (1 M, pH 6.8), Leupeptin (10 µg/mL), Aprotinin (5 µg/mL), phenylmethylsulfonyl fluoride (50 µM), EDTA (0.5 M), EGTA (0.5 M), phosphatase inhibitor cocktail II (5 mg/mL, Sigma-Aldrich), and sodium dodecyl sulfate (SDS) 10%. .. The antibodies anti-actin goat polyclonal (Santa Cruz Biotechnology), anti–glial fibrillary acidic protein (GFAP) monoclonal (Immunological Sciences), anti-vimentin monoclonal (Dako), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonal (Chemicon), anti-Hes1 polyclonal (Chemicon), anti-nestin polyclonal (Abcam), and anti-CD133 polyclonal (Abcam) were used at dilutions of 1:1000, 1:500, 1:25, 1:1000, 1:200, 1:500, and 1 µg/mL, respectively.

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    Millipore rabbit anti bax
    Effects of coffee oil-algae oil nanoemulsions on protein expressions of <t>cyclin</t> B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and <t>Bax,</t> Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p
    Rabbit Anti Bax, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax/product/Millipore
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    91
    Millipore rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to <t>glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH),</t> which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.
    Rabbit Polyclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    82
    Millipore rabbit anti bax antibody
    Relationship of BNIP3 expression to <t>Bax</t> activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control <t>COX</t> IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.
    Rabbit Anti Bax Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax antibody/product/Millipore
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti bax antibody - by Bioz Stars, 2020-04
    82/100 stars
      Buy from Supplier

    Image Search Results


    Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

    doi: 10.2147/IJN.S144705

    Figure Lengend Snippet: Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Article Snippet: The primary antibodies include mouse monoclonal anti-α-tubulin antibody (Sigma–Aldrich Co.), mouse anti-cyclin A and rabbit anti-Bax (EMD Millipore), mouse anti-CDK2, mouse anti-cytochrome C, mouse anti-p21, mouse anti-cyclin B, and mouse anti-Bcl-2 (BD Biosciences), as well as anti-cdc2 (CDK1) and mouse anti-p53 (Novus Biologicals Co., Littleton, CO, USA).

    Techniques: Incubation, Standard Deviation

    L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Journal: BMC Medicine

    Article Title: Sodium vanadate combined with l-ascorbic acid delays disease progression, enhances motor performance, and ameliorates muscle atrophy and weakness in mice with spinal muscular atrophy

    doi: 10.1186/1741-7015-11-38

    Figure Lengend Snippet: L-Ascorbic acid (L-AA) eliminates sodium vanadate (SV)-induced cytotoxicity . (A) Western blots showing survival motor neuron (SMN) expression in SMN2 -NSC34 cells treated with 400 μM L -AA, 200 μM SV, or SV combined with L -AA at different time points. β-Actin was used as an internal control. (B-D) Quantification of SMN protein expression in (A). (E-G) Quantification of the viability of SMN2 -NSC34 cells (E) and human dermal fibroblasts (HDFs) (F, G) treated with L -AA, SV or SV combined with L -AA. (H, I) Western blots showing SMN and B cell lymphoma 2-associated X protein (Bax) expression in SMN2 -NSC34 cells (H) and HDFs (I) treated with vehicle, L -AA, SV or SV combined with L -AA. β-Actin was used as an internal control. (J, K) Quantification of SMN and Bax expression in (H). (L, M) Quantification of SMN and Bax expression in (I). The experiment was repeated at least three times, and the mean ± SEM was calculated. Statistical comparisons were performed by one-way analysis of variance (ANOVA) (E-G) and Student's t test (B-D, and J-M).* P

    Article Snippet: Primary antibodies used for western blotting included mouse anti-SMN (1:5,000; BD Biosciences, San Diego, CA, USA), mouse anti-β-actin (1:10,000; Sigma), rabbit anti-Bax (1:1,000; Millipore, Temecula, CA, USA), and rabbit anti-caspase 3 (1:1,000; Cell Signaling, Temecula, CA, USA).

    Techniques: Western Blot, Expressing

    Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Journal: Acta Physiologica (Oxford, England)

    Article Title: Impaired structural and functional regeneration of skeletal muscles from β2-adrenoceptor knockout mice

    doi: 10.1111/apha.12329

    Figure Lengend Snippet: Smad4 and akirin1 expression. (a) Representative Western blots of contralateral muscles (CL) and injured muscles analysed on post-cryolesion days 3 and 10 (Cryo 3d; Cryo 10d) from WT and β 2 KO mice. Blots (50 μ g per lane) were reacted with antibodies specific to Smad4 and akirin1 (7 and 12% SDS-PAGE gels respectively), and subsequently with antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which was used as standard. MW, molecular weight. (b) Densitometry analyses of Smad4 and akirin1. Data are presented as mean and SD of arbitrary unit (A.U.). n = 3 (analysis of variance followed by Bonferroni's test for multiple comparisons). * P ≤ 0.05 vs. CL; # P ≤ 0.05 vs. WT mice.

    Article Snippet: Antibodies for Western blotting The primary antibodies used for Western blotting were (1) rabbit polyclonal anti-Smad4 (1 : 1000; cat# 9515; Cell Signaling Technology), (2) rabbit polyclonal anti-akirin1 (1 : 1000; cat# ab77075; Abcam) and (3) rabbit polyclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1 : 2000; cat# ABS16; Millipore, Temecula, CA, USA).

    Techniques: Expressing, Western Blot, Mouse Assay, SDS Page, Molecular Weight

    Relationship of BNIP3 expression to Bax activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control COX IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Cyanide-Induced Apoptosis of Dopaminergic Cells Is Promoted by BNIP3 and Bax Modulation of Endoplasmic Reticulum-Mitochondrial Ca2+ Levels S⃞

    doi: 10.1124/jpet.109.159103

    Figure Lengend Snippet: Relationship of BNIP3 expression to Bax activation. A, cells were transfected with control or BNIP3 siRNA and 24 h later were treated with KCN (400 μM) for 12 h. Bax expression in mitochondria (HM) and ER (LM) was detected by Western blotting. Densitometric data were normalized with the loading control COX IV (mitochondria) or calnexin (ER) and represent the mean ± S.E.M. of three separate determinations. #, Significantly different from the KCN + control siRNA group in HM; , significantly different from the KCN + control siRNA group in LM. B, cells were transfected with wild-type BNIP3 cDNA, and 24 h later, Bax and BNIP3 expression was determined by Western blotting in HM and LM fractions. EV = empty vector.

    Article Snippet: The primary antibodies were: mouse anti-BNIP3 antibody, mouse anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO), mouse anti-cytochrome c oxidase (COX IV), rabbit anticalnexin antibodies (Abcam Inc., Cambridge, MA), rabbit anti-Bax antibody (Millipore, Billerica, MA), and mouse anti-Bax antibody (Santa Cruz Biotechnology, San Cruz, CA).

    Techniques: Expressing, Activation Assay, Transfection, Western Blot, Plasmid Preparation