Structured Review

Abcam rabbit anti glutathione peroxidase 1
Gene expression (A) and immunohistochemical staining (B–E) of glutathione peroxidase 1 in the airways of filtered air (B and D) or DFP exposed (C and E) neonatal (B and C) or adult rats (D and E). Gene expression of glutathione peroxidase 1 (A)
Rabbit Anti Glutathione Peroxidase 1, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti glutathione peroxidase 1/product/Abcam
Average 95 stars, based on 2 article reviews
Price from $9.99 to $1999.99
rabbit anti glutathione peroxidase 1 - by Bioz Stars, 2022-09
95/100 stars

Images

1) Product Images from "Age specific responses to acute inhalation of diffusion flame soot particles: Cellular injury and the airway antioxidant response"

Article Title: Age specific responses to acute inhalation of diffusion flame soot particles: Cellular injury and the airway antioxidant response

Journal: Inhalation toxicology

doi: 10.3109/08958378.2010.513403

Gene expression (A) and immunohistochemical staining (B–E) of glutathione peroxidase 1 in the airways of filtered air (B and D) or DFP exposed (C and E) neonatal (B and C) or adult rats (D and E). Gene expression of glutathione peroxidase 1 (A)
Figure Legend Snippet: Gene expression (A) and immunohistochemical staining (B–E) of glutathione peroxidase 1 in the airways of filtered air (B and D) or DFP exposed (C and E) neonatal (B and C) or adult rats (D and E). Gene expression of glutathione peroxidase 1 (A)

Techniques Used: Expressing, Immunohistochemistry, Staining

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    Abcam horseradish peroxidase hrp conjugated anti glutathione s transferase gst antibodies
    Binding of the identified compounds to the p21-binding domain (PBD). ( a ) Binding sensorgram for compound 2 . PBD or <t>glutathione</t> S -transferase <t>(GST;</t> control) was immobilized on the gold surface, and then various concentrations of compound 2 were passed over the immobilized PBD or GST (RU: response unit). ( b ) The K d values for the compound–PBD interactions as measured by SPR. ( c ) Effect of a mutation in the CRIB motif on the interaction of PBD with compound 2 . Compound 2 (100 μ M ) was passed over the immobilized wild-type PBD (Wt-PBD) or the PBD with two mutations (H83L/H86L; PBD-LL). All data are representative of at least three independent experiments.
    Horseradish Peroxidase Hrp Conjugated Anti Glutathione S Transferase Gst Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated anti glutathione s transferase gst antibodies/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated anti glutathione s transferase gst antibodies - by Bioz Stars, 2022-09
    94/100 stars
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    93
    Abcam anti gpx 1
    Crocin reduces UVB-induced intracellular ROS. (A) Intracellular ROS measured by DCFH 2 -DA staining at 20 min post-UVB irradiation. Scale bars, 500 µm. (B) Intracellular ROS measured by DCFH2-DA staining and flow cytometry. (C) Antioxidant effect of crocin measured by 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity. (D) Western blot analysis of (E) <t>GPX-1</t> expression at 72 h post-UVB irradiation. *P
    Anti Gpx 1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gpx 1/product/Abcam
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    anti gpx 1 - by Bioz Stars, 2022-09
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    Image Search Results


    Binding of the identified compounds to the p21-binding domain (PBD). ( a ) Binding sensorgram for compound 2 . PBD or glutathione S -transferase (GST; control) was immobilized on the gold surface, and then various concentrations of compound 2 were passed over the immobilized PBD or GST (RU: response unit). ( b ) The K d values for the compound–PBD interactions as measured by SPR. ( c ) Effect of a mutation in the CRIB motif on the interaction of PBD with compound 2 . Compound 2 (100 μ M ) was passed over the immobilized wild-type PBD (Wt-PBD) or the PBD with two mutations (H83L/H86L; PBD-LL). All data are representative of at least three independent experiments.

    Journal: Experimental & Molecular Medicine

    Article Title: Small molecules that allosterically inhibit p21-activated kinase activity by binding to the regulatory p21-binding domain

    doi: 10.1038/emm.2016.13

    Figure Lengend Snippet: Binding of the identified compounds to the p21-binding domain (PBD). ( a ) Binding sensorgram for compound 2 . PBD or glutathione S -transferase (GST; control) was immobilized on the gold surface, and then various concentrations of compound 2 were passed over the immobilized PBD or GST (RU: response unit). ( b ) The K d values for the compound–PBD interactions as measured by SPR. ( c ) Effect of a mutation in the CRIB motif on the interaction of PBD with compound 2 . Compound 2 (100 μ M ) was passed over the immobilized wild-type PBD (Wt-PBD) or the PBD with two mutations (H83L/H86L; PBD-LL). All data are representative of at least three independent experiments.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-glutathione S -transferase (GST) antibodies were purchased from Abcam (Cambridge, MA, USA), anti-phospho-PAK antibodies from Cell Signaling Technology, Inc. (Danvers, MA, USA), anti-total myelin basic protein (MBP) antibodies from Millipore (Billerica, MA, USA) and anti-myc antibodies from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

    Techniques: Binding Assay, SPR Assay, Mutagenesis

    Crocin reduces UVB-induced intracellular ROS. (A) Intracellular ROS measured by DCFH 2 -DA staining at 20 min post-UVB irradiation. Scale bars, 500 µm. (B) Intracellular ROS measured by DCFH2-DA staining and flow cytometry. (C) Antioxidant effect of crocin measured by 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity. (D) Western blot analysis of (E) GPX-1 expression at 72 h post-UVB irradiation. *P

    Journal: Molecular Medicine Reports

    Article Title: Protective effect of crocin on ultraviolet B-induced dermal fibroblast photoaging

    doi: 10.3892/mmr.2018.9150

    Figure Lengend Snippet: Crocin reduces UVB-induced intracellular ROS. (A) Intracellular ROS measured by DCFH 2 -DA staining at 20 min post-UVB irradiation. Scale bars, 500 µm. (B) Intracellular ROS measured by DCFH2-DA staining and flow cytometry. (C) Antioxidant effect of crocin measured by 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity. (D) Western blot analysis of (E) GPX-1 expression at 72 h post-UVB irradiation. *P

    Article Snippet: The membranes were blocked with 5% non-fat milk in Tris-buffered saline and Tween 20 (TBST) at room temperature for 2 h, followed by incubation with the following primary antibodies overnight at 4°C: Anti-Col-1 (cat. no. ab6308; 1:1,000; Abcam, Cambridge, UK), anti-GPX-1 (cat. no. ab108427; 1:2,000; Abcam) and β-actin (cat. no. 4970S; 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Staining, Irradiation, Flow Cytometry, Cytometry, Activity Assay, Western Blot, Expressing

    Knockdown of ELAVL1 suppresses I/R induced ferroptosis and injury via inhibiting autophagy. For inhibition of ELAVL1 or overexpression of Beclin-1, mice were received injection of lenti-si-ELAVL1 or Beclin-1. a Relative mRNA levels of ELAVL1 and Beclin-1 were measured by qRT-PCR. b – f Relative protein levels of p62, GPx4, Beclin-1, and LC3 were determined by western blotting. Relative iron level g , LDH activity h and LPO level i were assessed by corresponding assay kits. j Representative TTC staining images of myocardial sections from each group of mice. k Quantifications of infarct size in each group of mice. The results were presented as the mean ± SD. n = 5; * P

    Journal: Molecular Medicine

    Article Title: ELAVL1 is transcriptionally activated by FOXC1 and promotes ferroptosis in myocardial ischemia/reperfusion injury by regulating autophagy

    doi: 10.1186/s10020-021-00271-w

    Figure Lengend Snippet: Knockdown of ELAVL1 suppresses I/R induced ferroptosis and injury via inhibiting autophagy. For inhibition of ELAVL1 or overexpression of Beclin-1, mice were received injection of lenti-si-ELAVL1 or Beclin-1. a Relative mRNA levels of ELAVL1 and Beclin-1 were measured by qRT-PCR. b – f Relative protein levels of p62, GPx4, Beclin-1, and LC3 were determined by western blotting. Relative iron level g , LDH activity h and LPO level i were assessed by corresponding assay kits. j Representative TTC staining images of myocardial sections from each group of mice. k Quantifications of infarct size in each group of mice. The results were presented as the mean ± SD. n = 5; * P

    Article Snippet: Primary antibodies used in the study were: Anti-ELAVL1 (1:1000; Abcam, USA); Anti-GPx4 (1:5000; Abcam); Anti-ferritin heavy chain (FTH1, 1:1000; Abcam); Anti-Beclin-1 (1:1000; Santa Cruz, USA); Anti-p62 (1:1000; Abcam); Anti-LC3 (1:3000; Abcam); Anti-FOXC1 (1:1000; Abcam); Anti-β-actin (1: 1000; Santa Cruz).

    Techniques: Inhibition, Over Expression, Mouse Assay, Injection, Quantitative RT-PCR, Western Blot, Activity Assay, Staining

    Myocardial I/R induces ferroptosis and increases ELAVL1 expression level. Mice were subjected to myocardial I/R or sham surgery. a Representative TTC staining images of myocardial sections from sham-surgery mice and I/R surgery mice. b Quantifications of infarct size in each group. c Relative LDH activity in sham-surgery group and I/R group. d , e Relative protein levels of ELAVL1, GPx4, and FTH1 in sham-surgery group and I/R group. f Relative cellular iron levels in sham-surgery group and I/R group. g Relative GPx4 activity in sham-surgery group and I/R group. h Relative cellular GSH level in sham-surgery group and I/R group. The results were presented as the mean ± SD. n = 5; ** P

    Journal: Molecular Medicine

    Article Title: ELAVL1 is transcriptionally activated by FOXC1 and promotes ferroptosis in myocardial ischemia/reperfusion injury by regulating autophagy

    doi: 10.1186/s10020-021-00271-w

    Figure Lengend Snippet: Myocardial I/R induces ferroptosis and increases ELAVL1 expression level. Mice were subjected to myocardial I/R or sham surgery. a Representative TTC staining images of myocardial sections from sham-surgery mice and I/R surgery mice. b Quantifications of infarct size in each group. c Relative LDH activity in sham-surgery group and I/R group. d , e Relative protein levels of ELAVL1, GPx4, and FTH1 in sham-surgery group and I/R group. f Relative cellular iron levels in sham-surgery group and I/R group. g Relative GPx4 activity in sham-surgery group and I/R group. h Relative cellular GSH level in sham-surgery group and I/R group. The results were presented as the mean ± SD. n = 5; ** P

    Article Snippet: Primary antibodies used in the study were: Anti-ELAVL1 (1:1000; Abcam, USA); Anti-GPx4 (1:5000; Abcam); Anti-ferritin heavy chain (FTH1, 1:1000; Abcam); Anti-Beclin-1 (1:1000; Santa Cruz, USA); Anti-p62 (1:1000; Abcam); Anti-LC3 (1:3000; Abcam); Anti-FOXC1 (1:1000; Abcam); Anti-β-actin (1: 1000; Santa Cruz).

    Techniques: Expressing, Mouse Assay, Staining, Activity Assay

    Knockdown of ELAVL1 suppresses ferroptosis and H/R injury. Myocardial cells were transfected with si-ELAVL1 or si-NC and then subjected to H/R. a Relative mRNA levels of ELAVL1 and GPx4 in transfected myocardial cells following H/R exposure. b , c Relative protein levels of ELAVL1 and GPx4 in transfected myocardial cells following H/R exposure. d CCK8 assay to assess viability of transfected cells upon H/R exposure. e , f Relative cellular ROS levels were measured in each group of cells. Relative cellular iron level ( g ), LDH activity ( h ), LPO level ( i ) and GSH level ( j ) in each group were detected respectively. The results were presented as the mean ± SD. n = 3; * P

    Journal: Molecular Medicine

    Article Title: ELAVL1 is transcriptionally activated by FOXC1 and promotes ferroptosis in myocardial ischemia/reperfusion injury by regulating autophagy

    doi: 10.1186/s10020-021-00271-w

    Figure Lengend Snippet: Knockdown of ELAVL1 suppresses ferroptosis and H/R injury. Myocardial cells were transfected with si-ELAVL1 or si-NC and then subjected to H/R. a Relative mRNA levels of ELAVL1 and GPx4 in transfected myocardial cells following H/R exposure. b , c Relative protein levels of ELAVL1 and GPx4 in transfected myocardial cells following H/R exposure. d CCK8 assay to assess viability of transfected cells upon H/R exposure. e , f Relative cellular ROS levels were measured in each group of cells. Relative cellular iron level ( g ), LDH activity ( h ), LPO level ( i ) and GSH level ( j ) in each group were detected respectively. The results were presented as the mean ± SD. n = 3; * P

    Article Snippet: Primary antibodies used in the study were: Anti-ELAVL1 (1:1000; Abcam, USA); Anti-GPx4 (1:5000; Abcam); Anti-ferritin heavy chain (FTH1, 1:1000; Abcam); Anti-Beclin-1 (1:1000; Santa Cruz, USA); Anti-p62 (1:1000; Abcam); Anti-LC3 (1:3000; Abcam); Anti-FOXC1 (1:1000; Abcam); Anti-β-actin (1: 1000; Santa Cruz).

    Techniques: Transfection, CCK-8 Assay, Activity Assay

    Inhibition of autophagy suppresses H/R induced ferroptosis and cell injury. Myocardial cells were pretreated with 3-MA (5 mM) for 24 h before being subjected to H/R. a , b Relative protein levels of Beclin-1, p62 and LC3 were determined by western blotting. c – e Relative mRNA and protein levels of GPx4 in cells were assessed respectively. g CCK8 assay was performed to assess cell viability. (G H) Relative cellular ROS levels in 3-MA treated cells were measured after H/R. Relative cellular iron level ( i ), LDH activity ( j ), LPO level ( k ) and GSH level ( l ) in each group of cells were detected by corresponding assay kits after H/R. The results were presented as the mean ± SD. n = 3; * P

    Journal: Molecular Medicine

    Article Title: ELAVL1 is transcriptionally activated by FOXC1 and promotes ferroptosis in myocardial ischemia/reperfusion injury by regulating autophagy

    doi: 10.1186/s10020-021-00271-w

    Figure Lengend Snippet: Inhibition of autophagy suppresses H/R induced ferroptosis and cell injury. Myocardial cells were pretreated with 3-MA (5 mM) for 24 h before being subjected to H/R. a , b Relative protein levels of Beclin-1, p62 and LC3 were determined by western blotting. c – e Relative mRNA and protein levels of GPx4 in cells were assessed respectively. g CCK8 assay was performed to assess cell viability. (G H) Relative cellular ROS levels in 3-MA treated cells were measured after H/R. Relative cellular iron level ( i ), LDH activity ( j ), LPO level ( k ) and GSH level ( l ) in each group of cells were detected by corresponding assay kits after H/R. The results were presented as the mean ± SD. n = 3; * P

    Article Snippet: Primary antibodies used in the study were: Anti-ELAVL1 (1:1000; Abcam, USA); Anti-GPx4 (1:5000; Abcam); Anti-ferritin heavy chain (FTH1, 1:1000; Abcam); Anti-Beclin-1 (1:1000; Santa Cruz, USA); Anti-p62 (1:1000; Abcam); Anti-LC3 (1:3000; Abcam); Anti-FOXC1 (1:1000; Abcam); Anti-β-actin (1: 1000; Santa Cruz).

    Techniques: Inhibition, Western Blot, CCK-8 Assay, Activity Assay

    Representative 2D-PAGE Western blot images of antioxidant proteins in wildtype and Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for antioxidant proteins. Pooled liver protein extracts (n = 12) were separated by 2D PAGE in triplicate and transferred onto nitrocellulose membrane. Each membrane was probed with one of the following primary antibodies: Sod1 (a, b), Gpx1 (c, d), or Prdx6 (e, f). The proteins of interest were detected with an HRP-conjugated secondary antibody and imaged on a LAS-3000 luminescent analyzer. Arrows indicate the protein spots that correspond to those identified by 2D-PAGE analysis.

    Journal: BioMed Research International

    Article Title: Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response

    doi: 10.1155/2018/4963942

    Figure Lengend Snippet: Representative 2D-PAGE Western blot images of antioxidant proteins in wildtype and Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for antioxidant proteins. Pooled liver protein extracts (n = 12) were separated by 2D PAGE in triplicate and transferred onto nitrocellulose membrane. Each membrane was probed with one of the following primary antibodies: Sod1 (a, b), Gpx1 (c, d), or Prdx6 (e, f). The proteins of interest were detected with an HRP-conjugated secondary antibody and imaged on a LAS-3000 luminescent analyzer. Arrows indicate the protein spots that correspond to those identified by 2D-PAGE analysis.

    Article Snippet: Cell lysates were harvested and 40 μ g of cell lysate protein was subject to western blot analysis with anti-Gpx1 (ab108427, Abcam), anti-Prdx6 (ab16947 Abcam), and anti-SOD (ab13498 Abcam) antibodies.

    Techniques: Polyacrylamide Gel Electrophoresis, Western Blot, Mouse Assay

    Lp(a) upregulates GPx1 and Prdx6 expression in human HepG2 cells. HepG2 cells were treated with 5 μ g/mL of Lp(a) or LDL for 6 hours at 37°C. Western blots of cell lysates were performed with an anti-Gpx1 antibody (a), an anti-Prdx6 antibody (b), an anti-Sod1 antibody (c), and an anti-Park7 antibody (d) using an anti-actin antibody as a loading control. Representative blots are shown. Protein levels were normalized against actin and expressed relative to that of untreated cells. Results are expressed as mean ± SEM for pooled triplicate incubations run in quadruplicate. ∗ P

    Journal: BioMed Research International

    Article Title: Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response

    doi: 10.1155/2018/4963942

    Figure Lengend Snippet: Lp(a) upregulates GPx1 and Prdx6 expression in human HepG2 cells. HepG2 cells were treated with 5 μ g/mL of Lp(a) or LDL for 6 hours at 37°C. Western blots of cell lysates were performed with an anti-Gpx1 antibody (a), an anti-Prdx6 antibody (b), an anti-Sod1 antibody (c), and an anti-Park7 antibody (d) using an anti-actin antibody as a loading control. Representative blots are shown. Protein levels were normalized against actin and expressed relative to that of untreated cells. Results are expressed as mean ± SEM for pooled triplicate incubations run in quadruplicate. ∗ P

    Article Snippet: Cell lysates were harvested and 40 μ g of cell lysate protein was subject to western blot analysis with anti-Gpx1 (ab108427, Abcam), anti-Prdx6 (ab16947 Abcam), and anti-SOD (ab13498 Abcam) antibodies.

    Techniques: Expressing, Western Blot