anti glutaryl lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti glutaryl lysine
    Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, <xref ref-type=Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA. " width="250" height="auto" />
    Anti Glutaryl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Deglutarylation of glutaryl-CoA dehydrogenase by deacylating enzyme SIRT5 promotes lysine oxidation in mice"

    Article Title: Deglutarylation of glutaryl-CoA dehydrogenase by deacylating enzyme SIRT5 promotes lysine oxidation in mice

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2022.101723

    Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, <xref ref-type=Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA. " title="... and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA.

    Techniques Used: CRISPR, Western Blot

    pan glutaryl lysine ptm 1151  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pan glutaryl lysine ptm 1151
    Pan Glutaryl Lysine Ptm 1151, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti glutaryl lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti glutaryl lysine
    (A) Immunoblot for SIRT5 protein from HEK293T crWT (circles) clone, and crKO (blank) clone (using CRISPR guide E, table S1). (B) Relative SIRT5 protein quantification of (A) (mean ± SD, n=3). (C) Relative mRNA expression of mitochondrial sirtuins (SIRT3, 4 and 5) normalized to ATP subunit 5 mRNA levels in 293T SIRT5 crWT and crKO cells (mean ± SEM, n=5, *= p-value ≤ 0.05). (D-G) Immunoblots of SIRT5 crWT and crKO whole cell lysates blotted using antibodies against <t>glutaryl-lysine</t> (D), -succinyl-lysine (E), malonyl-lysine (F), and acetyl-lysine (G). (H-K) Relative quantification of acylated proteins normalized <t>to</t> <t>β-actin</t> levels: glutarylation (H), succinylation (I), malonylation (J), and acetylation (K) (mean ± SD, n=3).
    Anti Glutaryl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Deglutarylation of GCDH by SIRT5 controls lysine metabolism in mice"

    Article Title: Deglutarylation of GCDH by SIRT5 controls lysine metabolism in mice

    Journal: bioRxiv

    doi: 10.1101/2020.06.28.176677

    (A) Immunoblot for SIRT5 protein from HEK293T crWT (circles) clone, and crKO (blank) clone (using CRISPR guide E, table S1). (B) Relative SIRT5 protein quantification of (A) (mean ± SD, n=3). (C) Relative mRNA expression of mitochondrial sirtuins (SIRT3, 4 and 5) normalized to ATP subunit 5 mRNA levels in 293T SIRT5 crWT and crKO cells (mean ± SEM, n=5, *= p-value ≤ 0.05). (D-G) Immunoblots of SIRT5 crWT and crKO whole cell lysates blotted using antibodies against glutaryl-lysine (D), -succinyl-lysine (E), malonyl-lysine (F), and acetyl-lysine (G). (H-K) Relative quantification of acylated proteins normalized to β-actin levels: glutarylation (H), succinylation (I), malonylation (J), and acetylation (K) (mean ± SD, n=3).
    Figure Legend Snippet: (A) Immunoblot for SIRT5 protein from HEK293T crWT (circles) clone, and crKO (blank) clone (using CRISPR guide E, table S1). (B) Relative SIRT5 protein quantification of (A) (mean ± SD, n=3). (C) Relative mRNA expression of mitochondrial sirtuins (SIRT3, 4 and 5) normalized to ATP subunit 5 mRNA levels in 293T SIRT5 crWT and crKO cells (mean ± SEM, n=5, *= p-value ≤ 0.05). (D-G) Immunoblots of SIRT5 crWT and crKO whole cell lysates blotted using antibodies against glutaryl-lysine (D), -succinyl-lysine (E), malonyl-lysine (F), and acetyl-lysine (G). (H-K) Relative quantification of acylated proteins normalized to β-actin levels: glutarylation (H), succinylation (I), malonylation (J), and acetylation (K) (mean ± SD, n=3).

    Techniques Used: Western Blot, CRISPR, Expressing

    (A) Immunoblot for immuno-purified GCDH-FLAG by Flag-M2 resin from 293T SIRT5 crWT or crKO cells grown in complete media or acylation media (DMEM - glucose, - glutamine, - pyruvate, + 10% FBS). Blots are representative of at least three independent experiments and quantitative values of succinyl-lysine intensity normalized to FLAG intensity expressed as ratios relative to control (overexpressed GCDH-FLAG in SIRT5 crWT cells grown in complete media, lane 2. Same experiment as , blotted for Succ-K). (B) Immunoblot for immuno-purified GCDH-FLAG by Flag-M2 resin from SIRT5 crWT or crKO cells grown in complete media or glutarylation media (EBSS +50mM HEPES, +0.8mM Lysine) with or without co-expressed SIRT5-HA. Blots are representative of at least three independent experiments and quantitative values of glutaryl-lysine intensity normalized to FLAG intensity expressed as ratios relative to control (overexpressed GCDH-FLAG in SIRT5 crWT cells grown in complete media, lane 1. Same experiment as , blotted for Succ-K). (C) Immunoblot for immuno-purified GCDH-FLAG by Flag-M2 resin or succinyl-proteins by anti-succinyl-lysine antibody (Succ-K) from 293T SIRT5 CRISPR cells grown in complete media media. Blots representative of at least three independent experiments. (D) Immunoblot of immuno-purified GCDH-FLAG by Flag-M2 resin from 293T SIRT5 CRISPR cells grown in complete media with or without co-expressed SIRT5-HA. Blots representative of at least three independent experiments and quantitative values of succinyl-lysine intensity normalized to FLAG intensity expressed as rations relative to control (overexpressed GCDH-FLAG, lane 2. Same experiment as , blotted for Succ-K) (E) Immunoblot of chemically modified GCDH (hGCDH-6xHIS) using succinyl-CoA with or without incubation with recombinant SIRT5 (mSIRT5-6xHIS). Quantitative values of succinyl-lysine intensity normalized to total protein are expressed as ratios relative to control (succinyl-modified recombinant GCDH, lane 2) (F) Enzymatic activity of unmodified (black), succinylated (dark green) and desuccinylated (bright green) GCDH determined by using PMS/DCPIP as artificial electron acceptors and Succinyl-CoA as electron donor (mean ± SD, n=6, 8, and 7 for unmodified, modified and deacylated GCDH respectively; * = p-value ≤ 0.05). (G) Succinyl-K sites as identified by label-free quantitative LC-MS/MS on recombinant GCDH are mapped on full-length human GCDH protein.
    Figure Legend Snippet: (A) Immunoblot for immuno-purified GCDH-FLAG by Flag-M2 resin from 293T SIRT5 crWT or crKO cells grown in complete media or acylation media (DMEM - glucose, - glutamine, - pyruvate, + 10% FBS). Blots are representative of at least three independent experiments and quantitative values of succinyl-lysine intensity normalized to FLAG intensity expressed as ratios relative to control (overexpressed GCDH-FLAG in SIRT5 crWT cells grown in complete media, lane 2. Same experiment as , blotted for Succ-K). (B) Immunoblot for immuno-purified GCDH-FLAG by Flag-M2 resin from SIRT5 crWT or crKO cells grown in complete media or glutarylation media (EBSS +50mM HEPES, +0.8mM Lysine) with or without co-expressed SIRT5-HA. Blots are representative of at least three independent experiments and quantitative values of glutaryl-lysine intensity normalized to FLAG intensity expressed as ratios relative to control (overexpressed GCDH-FLAG in SIRT5 crWT cells grown in complete media, lane 1. Same experiment as , blotted for Succ-K). (C) Immunoblot for immuno-purified GCDH-FLAG by Flag-M2 resin or succinyl-proteins by anti-succinyl-lysine antibody (Succ-K) from 293T SIRT5 CRISPR cells grown in complete media media. Blots representative of at least three independent experiments. (D) Immunoblot of immuno-purified GCDH-FLAG by Flag-M2 resin from 293T SIRT5 CRISPR cells grown in complete media with or without co-expressed SIRT5-HA. Blots representative of at least three independent experiments and quantitative values of succinyl-lysine intensity normalized to FLAG intensity expressed as rations relative to control (overexpressed GCDH-FLAG, lane 2. Same experiment as , blotted for Succ-K) (E) Immunoblot of chemically modified GCDH (hGCDH-6xHIS) using succinyl-CoA with or without incubation with recombinant SIRT5 (mSIRT5-6xHIS). Quantitative values of succinyl-lysine intensity normalized to total protein are expressed as ratios relative to control (succinyl-modified recombinant GCDH, lane 2) (F) Enzymatic activity of unmodified (black), succinylated (dark green) and desuccinylated (bright green) GCDH determined by using PMS/DCPIP as artificial electron acceptors and Succinyl-CoA as electron donor (mean ± SD, n=6, 8, and 7 for unmodified, modified and deacylated GCDH respectively; * = p-value ≤ 0.05). (G) Succinyl-K sites as identified by label-free quantitative LC-MS/MS on recombinant GCDH are mapped on full-length human GCDH protein.

    Techniques Used: Western Blot, Purification, CRISPR, Modification, Incubation, Recombinant, Activity Assay, Liquid Chromatography with Mass Spectroscopy

    (A) Enzymatic activity of unmodified (black), glutarylated (dark purple) and de-glutarylated (light purple) GCDH determined by using ETF/DCPIP as an electron acceptors and Glutaryl-CoA as electron donor (mean ± SD, n = 4, 4, and 5 for unmodified, modified and deacylated GCDH respectively, *: p-value ≤ 0.05). (B) Enzymatic activity of unmodified (black), succinylated (dark green) and de-succinylated (light green) GCDH determined by using ETF/DCPIP as an electron acceptors and Succinyl-CoA as electron donor (mean ± SD, n = 4, 4, and 3 for unmodified, modified and deacylated GCDH respectively, *: p-value ≤ 0.05). (C) Immunoblot of blue-native PAGE separated GCDH tetramer and SDS-PAGE for denatured GCDH isolated from liver mitochondria of 24 h fed SIRT5WT and SIRT5KO mice. Fed GCDHKO mouse liver mitochondrial lysates used as control to identify correct GCDH tetramer complex. (D-E) Homotetrameric structure of human GCDH with each subunit colored separately (purple, blue, red, and green). Yellow sticks indicate modified lysine residues. The FAD cofactor and CoA substrate are black and light gray spheres, respectively. (F) Quantitation of GCDH tetramer intensity normalized to ETF complex intensity. Data representative of two independent experiments (mean ± SD, n = 3 for both SIRT5 WT and SIRT5 KO). (G) Far-UV CD spectra for glutarylated-GCDH (purple) and unmodified GCDH (black). (H) Thermal stability profiles for glutarylated-GCDH (open circles, purple curve) and unmodified GCDH (closed squares, dark curve). The solid lines represent two-state sigmoid curves from which the apparent midpoint temperature were determined. (I) Far-UV CD spectra for succinylated-GCDH (green) and unmodified GCDH (black). (J) Thermal stability profiles for succinylated-GCDH (open circles, green curve) and unmodified GCDH (closed squares, green curve). The solid lines represent two-state sigmoid curves from which the apparent midpoint temperature was determined.
    Figure Legend Snippet: (A) Enzymatic activity of unmodified (black), glutarylated (dark purple) and de-glutarylated (light purple) GCDH determined by using ETF/DCPIP as an electron acceptors and Glutaryl-CoA as electron donor (mean ± SD, n = 4, 4, and 5 for unmodified, modified and deacylated GCDH respectively, *: p-value ≤ 0.05). (B) Enzymatic activity of unmodified (black), succinylated (dark green) and de-succinylated (light green) GCDH determined by using ETF/DCPIP as an electron acceptors and Succinyl-CoA as electron donor (mean ± SD, n = 4, 4, and 3 for unmodified, modified and deacylated GCDH respectively, *: p-value ≤ 0.05). (C) Immunoblot of blue-native PAGE separated GCDH tetramer and SDS-PAGE for denatured GCDH isolated from liver mitochondria of 24 h fed SIRT5WT and SIRT5KO mice. Fed GCDHKO mouse liver mitochondrial lysates used as control to identify correct GCDH tetramer complex. (D-E) Homotetrameric structure of human GCDH with each subunit colored separately (purple, blue, red, and green). Yellow sticks indicate modified lysine residues. The FAD cofactor and CoA substrate are black and light gray spheres, respectively. (F) Quantitation of GCDH tetramer intensity normalized to ETF complex intensity. Data representative of two independent experiments (mean ± SD, n = 3 for both SIRT5 WT and SIRT5 KO). (G) Far-UV CD spectra for glutarylated-GCDH (purple) and unmodified GCDH (black). (H) Thermal stability profiles for glutarylated-GCDH (open circles, purple curve) and unmodified GCDH (closed squares, dark curve). The solid lines represent two-state sigmoid curves from which the apparent midpoint temperature were determined. (I) Far-UV CD spectra for succinylated-GCDH (green) and unmodified GCDH (black). (J) Thermal stability profiles for succinylated-GCDH (open circles, green curve) and unmodified GCDH (closed squares, green curve). The solid lines represent two-state sigmoid curves from which the apparent midpoint temperature was determined.

    Techniques Used: Activity Assay, Modification, Western Blot, Blue Native PAGE, SDS Page, Isolation, Quantitation Assay

    glutaryl lysine antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc glutaryl lysine antibody
    Glutaryl Lysine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monoclonal glutaryl lysine antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal glutaryl lysine antibodies
    Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids <t>in</t> <t>succinyl-CoA,</t> <t>glutaryl-CoA,</t> HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.
    Monoclonal Glutaryl Lysine Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Class of Reactive Acyl-CoA Species Reveals the Non-Enzymatic Origins of Protein Acylation"

    Article Title: A Class of Reactive Acyl-CoA Species Reveals the Non-Enzymatic Origins of Protein Acylation

    Journal: Cell metabolism

    doi: 10.1016/j.cmet.2017.03.006

    Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids in succinyl-CoA, glutaryl-CoA, HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.
    Figure Legend Snippet: Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids in succinyl-CoA, glutaryl-CoA, HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.

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    glutaryl lysine antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc glutaryl lysine antibody
    Glutaryl Lysine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monoclonal glutaryl lysine antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal glutaryl lysine antibodies
    Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids <t>in</t> <t>succinyl-CoA,</t> <t>glutaryl-CoA,</t> HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.
    Monoclonal Glutaryl Lysine Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Class of Reactive Acyl-CoA Species Reveals the Non-Enzymatic Origins of Protein Acylation"

    Article Title: A Class of Reactive Acyl-CoA Species Reveals the Non-Enzymatic Origins of Protein Acylation

    Journal: Cell metabolism

    doi: 10.1016/j.cmet.2017.03.006

    Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids in succinyl-CoA, glutaryl-CoA, HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.
    Figure Legend Snippet: Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids in succinyl-CoA, glutaryl-CoA, HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.

    Techniques Used:

    anti glutaryl lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti glutaryl lysine
    Anti Glutaryl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti glutaryl lysine  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti glutaryl lysine
    Anti Glutaryl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti glutaryl lysine
    Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, <xref ref-type=Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA. " width="250" height="auto" />
    Anti Glutaryl Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc pan glutaryl lysine ptm 1151
    Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, <xref ref-type=Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA. " width="250" height="auto" />
    Pan Glutaryl Lysine Ptm 1151, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc glutaryl lysine antibody
    Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, <xref ref-type=Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA. " width="250" height="auto" />
    Glutaryl Lysine Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutaryl lysine antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    glutaryl lysine antibody - by Bioz Stars, 2024-06
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    Cell Signaling Technology Inc monoclonal glutaryl lysine antibodies
    Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids <t>in</t> <t>succinyl-CoA,</t> <t>glutaryl-CoA,</t> HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.
    Monoclonal Glutaryl Lysine Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, <xref ref-type=Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA. " width="100%" height="100%">

    Journal: The Journal of Biological Chemistry

    Article Title: Deglutarylation of glutaryl-CoA dehydrogenase by deacylating enzyme SIRT5 promotes lysine oxidation in mice

    doi: 10.1016/j.jbc.2022.101723

    Figure Lengend Snippet: Characterization of the SIRT5 crKO cell line. A , schematic of the CRISPR-Cas9 system used to generate the SIRT5 crKO cell line. B , schematic of the human SIRT5 gene showing the sites of crRNA targets within Exon5/4 the four known SIRT5 coding transcripts in humans. C , immunoblot for SIRT5 protein from HEK293T crWT ( circles ) clone and crKO ( blank ) clone (using CRISPR guide E, Table S1 ). D – F , immunoblots of SIRT5 crWT and crKO whole-cell lysates blotted using antibodies against glutaryl-lysine ( D ), glutaryl-succinyl-lysine ( E ), and malonyl-lysine ( F ). G – I , relative quantification of acylated proteins normalized to β-actin levels: glutarylation ( G ), succinylation ( H ), and malonylation ( I ) (mean ± SD, n = 3, ∗ p -value ≤ 0.05). crRNA, crispr RNA; PAM, protospacer adjacent motif; SIRT5, sirtuin 5; tracrRNA, trans-activating crispr RNA.

    Article Snippet: Antibodies: anti-MG-lysine (Millipore-Sigma # ABS2120-25UG), anti-SIRT5 (Millipore-Sigma #HPA022002 [for human SIRT5] and Cell Signaling Technologies #8782 [for mouse SIRT5]), anti-GCDH (Millipore-Sigma # HPA048492), mouse anti-β-actin (Cell Signaling Technologies, #3700), rabbit anti-β-actin (Cell Signaling Technologies #8457), anti-glutaryl-lysine (Cell Signaling Technologies, noncommercial #14943MF and PTM-Biolabs #1151), anti-succinyl-lysine (Cell Signaling Technologies, noncommercial # 13599 and PTM-Biolabs #401), anti-malonyl-lysine (Cell Signaling Technologies #14942), anti-acetyl-lysine (Cell Signaling Technologies, #9441), IRDye 680RD donkey anti-mouse IgG (LI-COR #926-68072), and IRDye 800CW donkey anti-rabbit IgG (LI-COR #926-32213).

    Techniques: CRISPR, Western Blot

    Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids in succinyl-CoA, glutaryl-CoA, HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.

    Journal: Cell metabolism

    Article Title: A Class of Reactive Acyl-CoA Species Reveals the Non-Enzymatic Origins of Protein Acylation

    doi: 10.1016/j.cmet.2017.03.006

    Figure Lengend Snippet: Protein acylation by four- and five-carbon dicarboxylic acyl-CoAs. The terminal carboxylic acids in succinyl-CoA, glutaryl-CoA, HMG-CoA, and 3-methylglutaryl-CoA facilitate non-enzymatic lysine acylation through intramolecular general base catalysis-mediated formation of a highly reactive cyclic anhydride intermediate. In a mouse model of the human disorder HMG-CoA lyase deficiency, HMG-CoA and 3-methylglutaryl-CoA accumulate and, consequently, protein lysine HMGylation and 3-MGylation are elevated. In a mouse model of the human disorder glutaric academia (GA I), glutaryl-CoA accumulates and, consequently, protein lysine glutarylation is elevated.

    Article Snippet: Antibodies used were a polyclonal acetyl-lysine and monoclonal glutaryl-lysine antibodies (Cell Signaling, non-commercial), polyclonal succinyl-lysine, butyryl-lysine, and malonyl-lysine antibodies (PTM Biolabs, Inc.), and custom-made polyclonal glutaryl-lysine, succinyl-lysine, hydroxylmethylglutaryl-lysine (custom made from Yenzym Antibodies) and anti-myc (Santa Cruz Biotechnology).

    Techniques: