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American Research Products anti gapdh
Anti Gapdh, supplied by American Research Products, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gapdh/product/American Research Products
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
anti gapdh - by Bioz Stars, 2020-09
92/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿ †
Article Snippet: .. Whole-cell lysates prepared from transfected HEK293 or A549 cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblot analysis with the respective antibodies for ABCG2 (Kamiya Biomedical Company, Seattle, WA), GAPDH (American Research Products, Belmont, MA), and GFP (Cell Signaling Technology, Danvers, MA). .. The blot was analyzed with an Odyssey (Li-Cor, Licolin, NE) infrared imaging system after incubation with a 1:10,000 dilution of goat anti-mouse (IRDye800CW) or anti-rabbit (IRDye680) secondary antibody (Li-Cor).

Incubation:

Article Title: Irinotecan induces steroid and xenobiotic receptor (SXR) signaling to detoxification pathway in colon cancer cells
Article Snippet: .. The membranes were blocked with 5% defatted milk for 1 h, immunoblotted overnight at 4°C with anti-SXR (Santacruz Biotechnology, Santa Cruz, CA, USA or Abcam, Cambridge, MA, USA), anti-histone H3 (Cell Signaling, Beverly, MA, USA), anti-α-tubulin (Sigma), or anti-GAPDH (American Research Products, Belmont, MA, USA), then incubated with the secondary HRP-conjugated antibody. .. Bands were visualized using ECL detection reagents (Amersham Biosciences, Pittsburgh, PA, USA) and quantified with the ChemiDoc™ XRS imager (Bio-Rad, Hemel Hempstead, UK).

Transfection:

Article Title: Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿ †
Article Snippet: .. Whole-cell lysates prepared from transfected HEK293 or A549 cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblot analysis with the respective antibodies for ABCG2 (Kamiya Biomedical Company, Seattle, WA), GAPDH (American Research Products, Belmont, MA), and GFP (Cell Signaling Technology, Danvers, MA). .. The blot was analyzed with an Odyssey (Li-Cor, Licolin, NE) infrared imaging system after incubation with a 1:10,000 dilution of goat anti-mouse (IRDye800CW) or anti-rabbit (IRDye680) secondary antibody (Li-Cor).

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    American Research Products gapdh
    <t>YY1</t> transcription factor is involved in Cd-induced suppression of UBE2D2 gene expression in HK-2 cells. ( a ) High accuracy between the YY1 binding site and sequences upstream of UBE2D2 and UBE2D4 gene regions. Nucleotide abbreviation in the YY1 binding sequence is as follows: N is any nucleotide. ( b ) DNA binding activity of YY1 transcription factor in Cd-treated cells. ( c ) Knockdown efficiency of the YY1 gene in HK-2 cells by YY1 siRNA treatment. YY1 siRNA was added to HK-2 cells for 24 h. ( d , e ) mRNA levels of UBE2D2 and UBE2D4 in HK-2 cells treated with YY1 siRNA. YY1 siRNA was added to HK-2 cells for 24 h. ( f ) Real-time RT-PCR of YY1 gene expression. HK-2 cells were grown in six-well plates at a density of 2.0 × 10 4 cells/cm 2 and cultured for 48 h. Culture medium was discarded and the cells were treated with Cd (CdCl 2 ) in serum-free culture medium for 3 h. ( c – f ) mRNA levels were examined using real-time RT-PCR. Values are the mean ± S.D. ( n = 3). mRNA levels were normalized to <t>GAPDH</t> . *Significantly different from the control group, P
    Gapdh, supplied by American Research Products, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gapdh/product/American Research Products
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    gapdh - by Bioz Stars, 2020-09
    90/100 stars
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    YY1 transcription factor is involved in Cd-induced suppression of UBE2D2 gene expression in HK-2 cells. ( a ) High accuracy between the YY1 binding site and sequences upstream of UBE2D2 and UBE2D4 gene regions. Nucleotide abbreviation in the YY1 binding sequence is as follows: N is any nucleotide. ( b ) DNA binding activity of YY1 transcription factor in Cd-treated cells. ( c ) Knockdown efficiency of the YY1 gene in HK-2 cells by YY1 siRNA treatment. YY1 siRNA was added to HK-2 cells for 24 h. ( d , e ) mRNA levels of UBE2D2 and UBE2D4 in HK-2 cells treated with YY1 siRNA. YY1 siRNA was added to HK-2 cells for 24 h. ( f ) Real-time RT-PCR of YY1 gene expression. HK-2 cells were grown in six-well plates at a density of 2.0 × 10 4 cells/cm 2 and cultured for 48 h. Culture medium was discarded and the cells were treated with Cd (CdCl 2 ) in serum-free culture medium for 3 h. ( c – f ) mRNA levels were examined using real-time RT-PCR. Values are the mean ± S.D. ( n = 3). mRNA levels were normalized to GAPDH . *Significantly different from the control group, P

    Journal: Scientific Reports

    Article Title: Accumulation of p53 via down-regulation of UBE2D family genes is a critical pathway for cadmium-induced renal toxicity

    doi: 10.1038/srep21968

    Figure Lengend Snippet: YY1 transcription factor is involved in Cd-induced suppression of UBE2D2 gene expression in HK-2 cells. ( a ) High accuracy between the YY1 binding site and sequences upstream of UBE2D2 and UBE2D4 gene regions. Nucleotide abbreviation in the YY1 binding sequence is as follows: N is any nucleotide. ( b ) DNA binding activity of YY1 transcription factor in Cd-treated cells. ( c ) Knockdown efficiency of the YY1 gene in HK-2 cells by YY1 siRNA treatment. YY1 siRNA was added to HK-2 cells for 24 h. ( d , e ) mRNA levels of UBE2D2 and UBE2D4 in HK-2 cells treated with YY1 siRNA. YY1 siRNA was added to HK-2 cells for 24 h. ( f ) Real-time RT-PCR of YY1 gene expression. HK-2 cells were grown in six-well plates at a density of 2.0 × 10 4 cells/cm 2 and cultured for 48 h. Culture medium was discarded and the cells were treated with Cd (CdCl 2 ) in serum-free culture medium for 3 h. ( c – f ) mRNA levels were examined using real-time RT-PCR. Values are the mean ± S.D. ( n = 3). mRNA levels were normalized to GAPDH . *Significantly different from the control group, P

    Article Snippet: The membrane was probed with antibodies against p53 (1:1000; Cell Signaling Technology, Danvers, MA, USA), phospho-p53 (Ser15) (1:1000; Cell Signaling Technology), MDM2 (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), YY1 (1:200; Santa Cruz Biotechnology), FOXF1 (1:500; Santa Cruz Biotechnology), GAPDH (1:2000; American Research Products, Waltham, MA, USA) or Lamin A/C (1:1000; Cell Signaling Technology).

    Techniques: Expressing, Binding Assay, Sequencing, Activity Assay, Quantitative RT-PCR, Cell Culture

    FOXF1 transcription factor is involved in Cd-induced suppression of UBE2D4 gene expression in HK-2 cells. ( a ) High accuracy between the FOXF1 binding site and sequences upstream of UBE2D2 and UBE2D4 gene regions. Nucleotide abbreviation in the FOXF1 binding sequence is as follows: V, any nucleotide except T; N, any nucleotide; D, any nucleotide except C; R, A or G; and Y, C or T. ( b ) DNA binding activity of FOXF1 transcription factor in Cd-treated cells. ( c ) Knockdown efficiency of the FOXF1 gene in HK-2 cells by FOXF1 siRNA treatment. FOXF1 siRNA was added to HK-2 cells for 24 h. ( d , e ) mRNA levels of UBE2D2 and UBE2D4 in HK-2 cells treated with FOXF1 siRNA. FOXF1 siRNA was added to HK-2 cells for 24 h. ( f ) Real-time RT-PCR of FOXF1 gene expression. HK-2 cells were grown in six-well plates at a density of 2.0 × 10 4 cells/cm 2 and cultured for 48 h. Culture medium was discarded and the cells were treated with Cd (CdCl 2 ) in serum-free culture medium for 3 h. ( c – f ) mRNA levels were examined using real-time RT-PCR. Values are the mean ± S.D. ( n = 3). mRNA levels were normalized to GAPDH . *Significantly different from the control group, P

    Journal: Scientific Reports

    Article Title: Accumulation of p53 via down-regulation of UBE2D family genes is a critical pathway for cadmium-induced renal toxicity

    doi: 10.1038/srep21968

    Figure Lengend Snippet: FOXF1 transcription factor is involved in Cd-induced suppression of UBE2D4 gene expression in HK-2 cells. ( a ) High accuracy between the FOXF1 binding site and sequences upstream of UBE2D2 and UBE2D4 gene regions. Nucleotide abbreviation in the FOXF1 binding sequence is as follows: V, any nucleotide except T; N, any nucleotide; D, any nucleotide except C; R, A or G; and Y, C or T. ( b ) DNA binding activity of FOXF1 transcription factor in Cd-treated cells. ( c ) Knockdown efficiency of the FOXF1 gene in HK-2 cells by FOXF1 siRNA treatment. FOXF1 siRNA was added to HK-2 cells for 24 h. ( d , e ) mRNA levels of UBE2D2 and UBE2D4 in HK-2 cells treated with FOXF1 siRNA. FOXF1 siRNA was added to HK-2 cells for 24 h. ( f ) Real-time RT-PCR of FOXF1 gene expression. HK-2 cells were grown in six-well plates at a density of 2.0 × 10 4 cells/cm 2 and cultured for 48 h. Culture medium was discarded and the cells were treated with Cd (CdCl 2 ) in serum-free culture medium for 3 h. ( c – f ) mRNA levels were examined using real-time RT-PCR. Values are the mean ± S.D. ( n = 3). mRNA levels were normalized to GAPDH . *Significantly different from the control group, P

    Article Snippet: The membrane was probed with antibodies against p53 (1:1000; Cell Signaling Technology, Danvers, MA, USA), phospho-p53 (Ser15) (1:1000; Cell Signaling Technology), MDM2 (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), YY1 (1:200; Santa Cruz Biotechnology), FOXF1 (1:500; Santa Cruz Biotechnology), GAPDH (1:2000; American Research Products, Waltham, MA, USA) or Lamin A/C (1:1000; Cell Signaling Technology).

    Techniques: Expressing, Binding Assay, Sequencing, Activity Assay, Quantitative RT-PCR, Cell Culture

    Levels of ABCC4 in cells established using the Flp-In™ system. ABCC4 and GAPDH levels were determined by western blotting analysis with specific antibodies for ABCC4 and GAPDH and their levels were quantified using ImageJ (Wayne Rasband, Bethesda, MD, USA) as described in Materials and Methods. ABCC4-specific monoclonal antibody (M4I-10) or GAPDH-specific antibody was used for protein detection in PNGase F-treated cell lysate. The experiments were performed independently more than two times. Data are expressed as mean values ± S.D. (n = 3). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Journal: Cells

    Article Title: A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance

    doi: 10.3390/cells8010039

    Figure Lengend Snippet: Levels of ABCC4 in cells established using the Flp-In™ system. ABCC4 and GAPDH levels were determined by western blotting analysis with specific antibodies for ABCC4 and GAPDH and their levels were quantified using ImageJ (Wayne Rasband, Bethesda, MD, USA) as described in Materials and Methods. ABCC4-specific monoclonal antibody (M4I-10) or GAPDH-specific antibody was used for protein detection in PNGase F-treated cell lysate. The experiments were performed independently more than two times. Data are expressed as mean values ± S.D. (n = 3). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Article Snippet: The membranes were incubated with monoclonal anti-ABCC4 antibody (M4I-10; GeneTex Inc., Alton Parkway Irvine, CA, USA) or anti-GAPDH antibody (anti-GAPDH-Clone 6C5 mouse monoclonal, igG2b; American Research Products, Inc., Waltham, MA, USA) at 1:1000 dilution in TBST containing 5% (w /v ) skim milk powder for 1 h at room temperature with shaking after rinsing with TBST.

    Techniques: Western Blot

    Levels of ABCC4 mRNA in cells established using the Flp-In™ system. The levels of ABCC4 and GAPDH mRNA were measured using qPCR with specific primer sets for ABCC4 and GAPDH , as described in Materials and Methods. Data are calculated as ratios relative to the GAPDH mRNA levels in the cells and normalized to the ratio of ABCC4 / GAPDH . Data are expressed as mean values ± S.D. (n = 5). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Journal: Cells

    Article Title: A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance

    doi: 10.3390/cells8010039

    Figure Lengend Snippet: Levels of ABCC4 mRNA in cells established using the Flp-In™ system. The levels of ABCC4 and GAPDH mRNA were measured using qPCR with specific primer sets for ABCC4 and GAPDH , as described in Materials and Methods. Data are calculated as ratios relative to the GAPDH mRNA levels in the cells and normalized to the ratio of ABCC4 / GAPDH . Data are expressed as mean values ± S.D. (n = 5). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Article Snippet: The membranes were incubated with monoclonal anti-ABCC4 antibody (M4I-10; GeneTex Inc., Alton Parkway Irvine, CA, USA) or anti-GAPDH antibody (anti-GAPDH-Clone 6C5 mouse monoclonal, igG2b; American Research Products, Inc., Waltham, MA, USA) at 1:1000 dilution in TBST containing 5% (w /v ) skim milk powder for 1 h at room temperature with shaking after rinsing with TBST.

    Techniques: Real-time Polymerase Chain Reaction

    RT-PCR analysis investigating the relative abundances of different fragments of ABCG2 3′UTR in parental S1 and resistant S1MI80 cells. (A) Schematic showing the approximate positions of the six different overlapping fragments within the ABCG2 3′UTR analyzed by RT-PCR. Each fragment is about 500 bp long. (B) Relative abundances of different 3′UTR fragments were plotted relative to the amount in region A after normalization with GAPDH. Open bars, parental S1 cells; closed bars, resistant S1MI80 cells. The data shown represent the means ± SD for three independent experiments. Since the binding site for hsa-miR-519c is located at nt 3820 to 3841 within the ABCG2 3′UTR, only the long ABCG2 transcripts that can be regulated by hsa-miR-519c gave a PCR product for fragment F (nt 3775 to 4388). However, both the long and short ABCG2 transcripts produced PCR products for fragment A (nt 2462 to 3030). By comparing the PCR products for fragments A and F, we estimated that about 77% of ABCG2 transcripts in the parental S1 cells can be regulated by hsa-miR-519c. (C) Relative mRNA stabilities of the six different 3′UTR fragments. Total RNA was isolated at various time intervals after actinomycin D (5 μg/ml) treatment and analyzed for mRNA levels of the six 3′UTR regions by RT-PCR as described for panel A. The data from a representative experiment were plotted as the percentage of mRNA remaining for each 3′UTR fragment compared with the amount before the addition of actinomycin D, after normalization with GAPDH.

    Journal: Molecular and Cellular Biology

    Article Title: Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿ †

    doi: 10.1128/MCB.00331-08

    Figure Lengend Snippet: RT-PCR analysis investigating the relative abundances of different fragments of ABCG2 3′UTR in parental S1 and resistant S1MI80 cells. (A) Schematic showing the approximate positions of the six different overlapping fragments within the ABCG2 3′UTR analyzed by RT-PCR. Each fragment is about 500 bp long. (B) Relative abundances of different 3′UTR fragments were plotted relative to the amount in region A after normalization with GAPDH. Open bars, parental S1 cells; closed bars, resistant S1MI80 cells. The data shown represent the means ± SD for three independent experiments. Since the binding site for hsa-miR-519c is located at nt 3820 to 3841 within the ABCG2 3′UTR, only the long ABCG2 transcripts that can be regulated by hsa-miR-519c gave a PCR product for fragment F (nt 3775 to 4388). However, both the long and short ABCG2 transcripts produced PCR products for fragment A (nt 2462 to 3030). By comparing the PCR products for fragments A and F, we estimated that about 77% of ABCG2 transcripts in the parental S1 cells can be regulated by hsa-miR-519c. (C) Relative mRNA stabilities of the six different 3′UTR fragments. Total RNA was isolated at various time intervals after actinomycin D (5 μg/ml) treatment and analyzed for mRNA levels of the six 3′UTR regions by RT-PCR as described for panel A. The data from a representative experiment were plotted as the percentage of mRNA remaining for each 3′UTR fragment compared with the amount before the addition of actinomycin D, after normalization with GAPDH.

    Article Snippet: Whole-cell lysates prepared from transfected HEK293 or A549 cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblot analysis with the respective antibodies for ABCG2 (Kamiya Biomedical Company, Seattle, WA), GAPDH (American Research Products, Belmont, MA), and GFP (Cell Signaling Technology, Danvers, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Binding Assay, Polymerase Chain Reaction, Produced, Isolation

    (Top) ABCG2 mRNA is more stable in resistant S1MI80 cells than in parental S1 cells. Although ABCG2 is also upregulated by romidepsin treatment (2 ng/ml for 24 h), its mRNA stability is not affected. Nascent RNA synthesis was inhibited with actinomycin D (5 μg/ml), and RNAs were harvested at 0, 4, 8, and 16 h posttreatment. RT-PCR analysis of ABCG2 mRNA was carried out to trace the remaining amount of ABCG2 mRNA with time. c-myc and GAPDH mRNA levels were also monitored as controls for fast-degrading and stable mRNAs, respectively. The value recorded was the percentage of mRNA remaining compared with the amount before the addition of actinomycin D, after normalization with GAPDH. The data shown represent the means ± SD for three independent experiments. (Bottom) Representative gel image showing RT-PCR analysis of ABCG2, c-myc, and GAPDH in cells pretreated with actinomycin D.

    Journal: Molecular and Cellular Biology

    Article Title: Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿ †

    doi: 10.1128/MCB.00331-08

    Figure Lengend Snippet: (Top) ABCG2 mRNA is more stable in resistant S1MI80 cells than in parental S1 cells. Although ABCG2 is also upregulated by romidepsin treatment (2 ng/ml for 24 h), its mRNA stability is not affected. Nascent RNA synthesis was inhibited with actinomycin D (5 μg/ml), and RNAs were harvested at 0, 4, 8, and 16 h posttreatment. RT-PCR analysis of ABCG2 mRNA was carried out to trace the remaining amount of ABCG2 mRNA with time. c-myc and GAPDH mRNA levels were also monitored as controls for fast-degrading and stable mRNAs, respectively. The value recorded was the percentage of mRNA remaining compared with the amount before the addition of actinomycin D, after normalization with GAPDH. The data shown represent the means ± SD for three independent experiments. (Bottom) Representative gel image showing RT-PCR analysis of ABCG2, c-myc, and GAPDH in cells pretreated with actinomycin D.

    Article Snippet: Whole-cell lysates prepared from transfected HEK293 or A549 cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblot analysis with the respective antibodies for ABCG2 (Kamiya Biomedical Company, Seattle, WA), GAPDH (American Research Products, Belmont, MA), and GFP (Cell Signaling Technology, Danvers, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    hsa-miR-519c regulates endogenous ABCG2 expression in A549 cells. A549 cells were transfected with 0 to 120 nM of either specific hsa-miR-519c inhibitor, hsa-miR-519c mimic, or the respective negative control (based on the cel-miR-67 sequence [Dharmacon]; this miRNA has been confirmed to have minimal sequence homology with miRNAs in humans, mice, and rats) for 48 h. Total RNA and whole-cell lysates were harvested for subsequent RT-PCR and Western blot analysis of ABCG2 expression. ABCG2 expression was normalized with GAPDH and reported relative to that of mock-treated cells. The transcript for hsa-miR-519c was also measured by stem-loop RT-PCR. The figure shows a representative result for three independent and reproducible experiments. The universal miRNA negative control inhibitor or mimic did not affect ABCG2, hsa-miR-519c, or GAPDH expression.

    Journal: Molecular and Cellular Biology

    Article Title: Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿Regulation of ABCG2 Expression at the 3? Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line ▿ †

    doi: 10.1128/MCB.00331-08

    Figure Lengend Snippet: hsa-miR-519c regulates endogenous ABCG2 expression in A549 cells. A549 cells were transfected with 0 to 120 nM of either specific hsa-miR-519c inhibitor, hsa-miR-519c mimic, or the respective negative control (based on the cel-miR-67 sequence [Dharmacon]; this miRNA has been confirmed to have minimal sequence homology with miRNAs in humans, mice, and rats) for 48 h. Total RNA and whole-cell lysates were harvested for subsequent RT-PCR and Western blot analysis of ABCG2 expression. ABCG2 expression was normalized with GAPDH and reported relative to that of mock-treated cells. The transcript for hsa-miR-519c was also measured by stem-loop RT-PCR. The figure shows a representative result for three independent and reproducible experiments. The universal miRNA negative control inhibitor or mimic did not affect ABCG2, hsa-miR-519c, or GAPDH expression.

    Article Snippet: Whole-cell lysates prepared from transfected HEK293 or A549 cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblot analysis with the respective antibodies for ABCG2 (Kamiya Biomedical Company, Seattle, WA), GAPDH (American Research Products, Belmont, MA), and GFP (Cell Signaling Technology, Danvers, MA).

    Techniques: Expressing, Transfection, Negative Control, Sequencing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Levels of ABCC4 in cells established using the Flp-In™ system. ABCC4 and GAPDH levels were determined by western blotting analysis with specific antibodies for ABCC4 and GAPDH and their levels were quantified using ImageJ (Wayne Rasband, Bethesda, MD, USA) as described in Materials and Methods. ABCC4-specific monoclonal antibody (M4I-10) or GAPDH-specific antibody was used for protein detection in PNGase F-treated cell lysate. The experiments were performed independently more than two times. Data are expressed as mean values ± S.D. (n = 3). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Journal: Cells

    Article Title: A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance

    doi: 10.3390/cells8010039

    Figure Lengend Snippet: Levels of ABCC4 in cells established using the Flp-In™ system. ABCC4 and GAPDH levels were determined by western blotting analysis with specific antibodies for ABCC4 and GAPDH and their levels were quantified using ImageJ (Wayne Rasband, Bethesda, MD, USA) as described in Materials and Methods. ABCC4-specific monoclonal antibody (M4I-10) or GAPDH-specific antibody was used for protein detection in PNGase F-treated cell lysate. The experiments were performed independently more than two times. Data are expressed as mean values ± S.D. (n = 3). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Article Snippet: The membranes were incubated with monoclonal anti-ABCC4 antibody (M4I-10; GeneTex Inc., Alton Parkway Irvine, CA, USA) or anti-GAPDH antibody (anti-GAPDH-Clone 6C5 mouse monoclonal, igG2b; American Research Products, Inc., Waltham, MA, USA) at 1:1000 dilution in TBST containing 5% ( w / v ) skim milk powder for 1 h at room temperature with shaking after rinsing with TBST.

    Techniques: Western Blot

    Levels of ABCC4 mRNA in cells established using the Flp-In™ system. The levels of ABCC4 and GAPDH mRNA were measured using qPCR with specific primer sets for ABCC4 and GAPDH , as described in Materials and Methods. Data are calculated as ratios relative to the GAPDH mRNA levels in the cells and normalized to the ratio of ABCC4 / GAPDH . Data are expressed as mean values ± S.D. (n = 5). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Journal: Cells

    Article Title: A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance

    doi: 10.3390/cells8010039

    Figure Lengend Snippet: Levels of ABCC4 mRNA in cells established using the Flp-In™ system. The levels of ABCC4 and GAPDH mRNA were measured using qPCR with specific primer sets for ABCC4 and GAPDH , as described in Materials and Methods. Data are calculated as ratios relative to the GAPDH mRNA levels in the cells and normalized to the ratio of ABCC4 / GAPDH . Data are expressed as mean values ± S.D. (n = 5). Statistical analyses were performed using one-way ANOVA and Tukey’s HSD test (* p

    Article Snippet: The membranes were incubated with monoclonal anti-ABCC4 antibody (M4I-10; GeneTex Inc., Alton Parkway Irvine, CA, USA) or anti-GAPDH antibody (anti-GAPDH-Clone 6C5 mouse monoclonal, igG2b; American Research Products, Inc., Waltham, MA, USA) at 1:1000 dilution in TBST containing 5% ( w / v ) skim milk powder for 1 h at room temperature with shaking after rinsing with TBST.

    Techniques: Real-time Polymerase Chain Reaction