anti gabpa  (Thermo Fisher)


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    Name:
    anti GABPA
    Description:

    Catalog Number:
    PA5-26583
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    Structured Review

    Thermo Fisher anti gabpa
    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) <t>CTCF,</t> (B) <t>GABPA,</t> (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    https://www.bioz.com/result/anti gabpa/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gabpa - by Bioz Stars, 2021-07
    92/100 stars

    Images

    1) Product Images from "Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines"

    Article Title: Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines

    Journal: Genes, chromosomes & cancer

    doi: 10.1002/gcc.22735

    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.
    Figure Legend Snippet: Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    Techniques Used: Binding Assay, Positive Control, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines
    Article Snippet: Chromatin was sheared by sonication for 2x [4× 20s on/20s off] by the Bioruptor Pico (Diagenode, Denville, NJ). .. The following antibodies were used (10 µg per ChIP): anti-CTCF (#2899, Cell Signaling Technology, Danvers, CA), anti-MYC (#9402, Cell Signaling Technology), anti-GABPA (#27795, ThermoFisher), and anti-GSC (#40495, ThermoFisher). .. DNA was purified by MinElute PCR Purification Kit (Qiagen, Hilden, Germany).

    Labeling:

    Article Title: Therapeutic Delivery of miR-200c Enhances Radiosensitivity in Lung Cancer
    Article Snippet: Protein lysates (40 μg) were resolved on denaturing gels with 4–20% sodium dodecyl sulfate-polyacrylamide and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). .. Membranes were probed with the following antibodies: primary antibodies, anti-PRDX2 (Lab Frontier, Seoul, Korea), anti-GABP/Nrf2 (Pierce-Thermo Fisher Scientific, Rockford, IL), anti-SESN1 (Novus Biologicals, Littleton, CO), anti-ZEB1, anti-p21, anti-RAD51 (Cell Signaling Technologies, Beverly, MA), anti-H2AX (Millipore), anti-actin, anti-vinculin (Sigma), and secondary antibody labeled by horseradish peroxidase (Amersham GE Healthcare). .. The secondary antibody was visualized by using a chemiluminescent reagent Pierce ECL kit (Thermo Fisher Scientific, Waltham, MA).

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  • 92
    Thermo Fisher anti gabpa
    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) <t>CTCF,</t> (B) <t>GABPA,</t> (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.
    Anti Gabpa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gabpa/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gabpa - by Bioz Stars, 2021-07
    92/100 stars
      Buy from Supplier

    93
    Thermo Fisher antibody against nrf2a pa5 27735
    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) <t>CTCF,</t> (B) <t>GABPA,</t> (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.
    Antibody Against Nrf2a Pa5 27735, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against nrf2a pa5 27735/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against nrf2a pa5 27735 - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    Journal: Genes, chromosomes & cancer

    Article Title: Characterization of Human Telomerase Reverse Transcriptase Promoter Methylation and Transcription Factor Binding in Differentiated Thyroid Cancer Cell Lines

    doi: 10.1002/gcc.22735

    Figure Lengend Snippet: Transcription factor binding varies at the TERT promoter in DTC cell lines and normal thyroid tissue. Binding was measured relative to 5% input chromatin, and binding at the TERT locus normalized to a positive control locus for each factor in the indicated cell lines and normal thyroid tissue by ChIP-qPCR for (A) CTCF, (B) GABPA, (C) MYC, at two positions- the minimal promoter binding site and the upstream binding site, (D) GSC. All error bars represent standard error within triplicates.

    Article Snippet: The following antibodies were used (10 µg per ChIP): anti-CTCF (#2899, Cell Signaling Technology, Danvers, CA), anti-MYC (#9402, Cell Signaling Technology), anti-GABPA (#27795, ThermoFisher), and anti-GSC (#40495, ThermoFisher).

    Techniques: Binding Assay, Positive Control, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction