Journal: Journal of Pain Research

Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

doi: 10.2147/JPR.S180165

Figure Lengend Snippet: Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

Article Snippet: Sections were blocked with 5% donkey serum for 30 minutes and incubated with GABA A α2R (AGA-002; Alomone Labs, Jerusalem, Israel) and SP (sc58591; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or GABA B R1 (ab90883; Abcam, Cambridge, MA, USA) and GFAP (a marker for SGCs activities, 3670S; Cell Signaling) at 4°C overnight, washed three times with 0.1 M PBS, and then incubated in donkey anti-mouse IgG conjugated Alexa Fluor488 (Thermo Fisher Scientific) and donkey anti-rabbit IgG conjugated Alexa Fluor594 (Thermo Fisher Scientific) for 2 hours under room temperature.

Techniques: Immunofluorescence

Journal: Journal of Pain Research

Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

doi: 10.2147/JPR.S180165

Figure Lengend Snippet: Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

Article Snippet: Sections were blocked with 5% donkey serum for 30 minutes and incubated with GABA A α2R (AGA-002; Alomone Labs, Jerusalem, Israel) and SP (sc58591; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or GABA B R1 (ab90883; Abcam, Cambridge, MA, USA) and GFAP (a marker for SGCs activities, 3670S; Cell Signaling) at 4°C overnight, washed three times with 0.1 M PBS, and then incubated in donkey anti-mouse IgG conjugated Alexa Fluor488 (Thermo Fisher Scientific) and donkey anti-rabbit IgG conjugated Alexa Fluor594 (Thermo Fisher Scientific) for 2 hours under room temperature.

Techniques: Expressing

Journal: Journal of Pain Research

Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

doi: 10.2147/JPR.S180165

Figure Lengend Snippet: Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

Article Snippet: The membrane was incubated with rabbit anti-GABA A α2R (1:1,000, AGA-002; Alomone Labs) and rabbit anti-GAB-A B R1 (1:1,000, ab90883; Abcam).

Techniques: Immunofluorescence

Journal: Journal of Pain Research

Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

doi: 10.2147/JPR.S180165

Figure Lengend Snippet: Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

Article Snippet: The membrane was incubated with rabbit anti-GABA A α2R (1:1,000, AGA-002; Alomone Labs) and rabbit anti-GAB-A B R1 (1:1,000, ab90883; Abcam).

Techniques: Expressing

Journal: Molecular Pain

Article Title: Decreased pain threshold and enhanced synaptic transmission in the anterior cingulate cortex of experimental hypothyroidism mice

doi: 10.1186/1744-8069-10-38

Figure Lengend Snippet: Expression of GABA A receptor. (A) Representative western-blot analysis of GABA A -α2 subunit in the ACC homogenates. (B) Levels of GABA A -α2 in saline control, vehicle-, T3-, and T4-treated HT mice. n = 5 in each group. * p < 0.05, ** p < 0.01 compared with saline control; # p < 0.05, ## p < 0.01 compared with vehicle-treated HT mice.

Article Snippet: Rabbit anti-GABA A -α2 antibody was purchased from Alomone labs (Jerusalem, Israel).

Techniques: Expressing, Western Blot

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Number of Bulbospinal Neurons with GABA A Subunit-Immunoreactivity

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques:

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Percentages of Bulbospinal Neurons with GABA A Subunit-Immunoreactivity

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques:

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Post Hoc Testing for GABA Aα1 between Sedentary and Active animals and Post Hoc Simple Main Effects testing within Rostrocaudal Level for GABA Aα2 expression (see text and for reference)

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques: Expressing

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Post Hoc Testing for Simple Main Effects within Rostrocaudal Level for the absolute numbers and percentages of GABA Aα2 -Immunoreactive (IR) C1 and non-C1 Neurons (see for reference)

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques:

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Primary Antibodies

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques: Purification

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Number of Bulbospinal Neurons with GABA A Subunit-Immunoreactivity

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques:

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Percentages of Bulbospinal Neurons with GABA A Subunit-Immunoreactivity

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques:

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Post Hoc Testing for GABA Aα1 between Sedentary and Active animals and Post Hoc Simple Main Effects testing within Rostrocaudal Level for GABA Aα2 expression (see text and for reference)

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques: Expressing

Journal: The Journal of comparative neurology

Article Title: Subregional Differences in GABA A Receptor Subunit Expression in the Rostral Ventrolateral Medulla of Sedentary Versus Physically Active Rats

doi: 10.1002/cne.24798

Figure Lengend Snippet: Post Hoc Testing for Simple Main Effects within Rostrocaudal Level for the absolute numbers and percentages of GABA Aα2 -Immunoreactive (IR) C1 and non-C1 Neurons (see for reference)

Article Snippet: GABA Aα2 , Amino acids 395-405 of rat GABA Aα2 , Alomone Labs, AGA-002, rabbit polyclonal , AB_2039864 , 1:200 (WB).

Techniques: