Structured Review

Millipore anti flag
Anti Flag, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore anti flag m2 affinity gel
A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in <t>M2</t> (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.
Anti Flag M2 Affinity Gel, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti flag m2 affinity gel/product/Millipore
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anti flag m2 affinity gel - by Bioz Stars, 2024-04
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Images

1) Product Images from "Regulation by the RNA-binding protein Unkempt at its effector interface"

Article Title: Regulation by the RNA-binding protein Unkempt at its effector interface

Journal: Nature Communications

doi: 10.1038/s41467-024-47449-4

A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in M2 (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.
Figure Legend Snippet: A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in M2 (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.

Techniques Used: Binding Assay, Recombinant, Incubation, Construct, Mutagenesis


Structured Review

Millipore anti flag antibody
Anti Flag Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti flag antibody/product/Millipore
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anti flag antibody - by Bioz Stars, 2024-04
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Structured Review

Millipore flag m2 antibody

Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flag m2 antibody/product/Millipore
Average 86 stars, based on 1 article reviews
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flag m2 antibody - by Bioz Stars, 2024-04
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Images

1) Product Images from "Regulation of transcription patterns, poly(ADP-ribose), and RNA-DNA hybrids by the ATM protein kinase"

Article Title: Regulation of transcription patterns, poly(ADP-ribose), and RNA-DNA hybrids by the ATM protein kinase

Journal: Cell reports

doi: 10.1016/j.celrep.2024.113896


Figure Legend Snippet:

Techniques Used: Recombinant, Protease Inhibitor, Binding Assay, Magnetic Beads, Single Cell Gel Electrophoresis, Multiplex Assay, Software, RNA Extraction


Structured Review

Millipore mouse anti flag m2 monoclonal antibody
Mouse Anti Flag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
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mouse anti flag m2 monoclonal antibody - by Bioz Stars, 2024-04
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Millipore mouse anti flag tag m2 monoclonal antibody
Mouse Anti Flag Tag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag tag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
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mouse anti flag tag m2 monoclonal antibody - by Bioz Stars, 2024-04
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Structured Review

Millipore mouse anti flag m2 monoclonal antibody
Mouse Anti Flag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag m2 monoclonal antibody - by Bioz Stars, 2024-04
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Structured Review

Millipore mouse anti flag tag m2 monoclonal antibody
Mouse Anti Flag Tag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag tag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag tag m2 monoclonal antibody - by Bioz Stars, 2024-04
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Millipore anti flag m2 magnetic beads
Anti Flag M2 Magnetic Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti flag m2 magnetic beads/product/Millipore
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anti flag m2 magnetic beads - by Bioz Stars, 2024-04
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Millipore mouse anti flag m2 antibody
Mouse Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag m2 antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag m2 antibody - by Bioz Stars, 2024-04
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  • 86
    Millipore anti flag
    Anti Flag, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag/product/Millipore
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    Millipore anti flag m2 affinity gel
    A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in <t>M2</t> (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.
    Anti Flag M2 Affinity Gel, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag m2 affinity gel/product/Millipore
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    Millipore anti flag antibody
    A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in <t>M2</t> (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.
    Anti Flag Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore flag m2 antibody

    Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mouse anti flag m2 monoclonal antibody

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    Mouse Anti Flag Tag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti flag m2 magnetic beads

    Anti Flag M2 Magnetic Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mouse anti flag m2 antibody

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    A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in M2 (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.

    Journal: Nature Communications

    Article Title: Regulation by the RNA-binding protein Unkempt at its effector interface

    doi: 10.1038/s41467-024-47449-4

    Figure Lengend Snippet: A Schematic representation of CCR4-NOT. Yellow circles indicate points of contact with UNK IDR identified in this study. The numbering of contacts indicates SLiM 1 or SLiM 2 binding sites. Adapted from Raisch et al. . under permission provided by a Creative Commons Attribution 4.0 International License. B Pull-down assays with recombinant UNK FULL tagged with the StrepII (Strep) affinity tag upon incubation with different CCR4-NOT modules ( n = 3). C As in ( B ) but with mutants of UNK IDR constructs fused to MBP and Strep after incubation with the NOT9 or the NOT module ( n = 3). D Twenty-five AlphaFold predictions of interfaces of UNK IDR interacting with the NOT9 module. The predictions are aligned on the CNOT9/CNOT1 heterodimer . The region of UNK IDR where the predictions converged is in dark blue. E The converged region of UNK IDR bound on the concave surface of CNOT9/CNOT1. F The same 25 predictions as in ( D ) but oriented to show the tryptophan (W)-binding pockets of CNOT9. G All 25 predictions of the converged region of UNK IDR close to the W-binding pockets of CNOT9. H Twenty-five AlphaFold predictions of UNK IDR interacting with the NOT module. The predictions are aligned on the CNOT1/CNOT2/CNOT3 heterotrimer . The region of UNK IDR where predictions converged is in dark blue. I The converged region of UNK IDR bound on the surface of CNOT1. J Pull-down of WT or M1-M3 mutants of the NOT9 module by MBP-UNK IDR -Strep. Residues in CNOT9 mutated to alanines in M1 (Y203 and R244) line the W-pocket 1, and those mutated in M2 (R205 and H208) line the W-pocket 2 . All residues (Y203, R205, H208, and R244) were mutated in M3 ( n = 2). K Pull-down of the WT or M3 NOT9 module or the NOT module by WT MBP-UNK IDR -Strep or its mutant with key residues in SLiM 1 (V511, I515, L522) substituted with glutamic acid. In ( E ), ( G ), and ( I ), the C-alpha atoms of the key interacting residues are shown as yellow spheres. The sequences of the converged region are shown with the key interacting residues in yellow.

    Article Snippet: The lysates were centrifuged at 17,000 × g for 30 min at 4 °C, then 250 μl anti-Flag M2 affinity gel (Millipore Sigma, A2220) was added and the mixture was rotated for 2 h at 4 °C.

    Techniques: Binding Assay, Recombinant, Incubation, Construct, Mutagenesis

    Journal: Cell reports

    Article Title: Regulation of transcription patterns, poly(ADP-ribose), and RNA-DNA hybrids by the ATM protein kinase

    doi: 10.1016/j.celrep.2024.113896

    Figure Lengend Snippet:

    Article Snippet: FLAG M2 antibody , Millipore Sigma , F1804.

    Techniques: Recombinant, Protease Inhibitor, Binding Assay, Magnetic Beads, Single Cell Gel Electrophoresis, Multiplex Assay, Software, RNA Extraction