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anti flag m2 f 3167  (Millipore)

 
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    Structured Review

    Millipore anti flag m2 f 3167
    Hippo-Kinase-Independent YAP Serine Phosphorylation Determines Impaired Nuclear Translocation and Blunted YAP-Dependent Transcriptional Activity in Cav1KO Cells (A) Western blot of Ser112-phopshorylated YAP and total YAP in WT and Cav1KO MEFs and Cav1KO MEFs reconstituted with CAV1 (o_CAV1) or IRES-GFP. (B) Confocal immunofluorescence of cells transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with <t>anti-FLAG</t> antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). (C) FLAG distribution from analysis as in (B), represented as the ratio of nuclear-to-cytosolic intensities. n = 6–11. (D) Western blot analysis of FLAG subcellular distribution in MEFs transfected with YAP-FLAG or YAP(S5A)-FLAG followed by biochemical fractionation. RHO-GDI and Tef-1 were used as cytosolic and nuclear markers, respectively. The percentage of total YAP located in the nuclear fractions was quantified (right graph). (E) TEAD transcriptional activity in MEFs transfected with YAP-FLAG and YAP(S5A)-FLAG, measured by 8xGTICC-luciferase reporter assay. Data are normalized to growth on the soft substrate in each experiment. n = 3 (E). The fold-change with respect to untransfected cells is indicated above the bars. (F) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or Lats1 and 2 siRNAs. Data are normalized to WT control. n = 10. (G) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or NF2 siRNAs. Data are normalized to WT control. n = 4. Data are presented as mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, and ∗∗∗∗ p < 0.0005. See also <xref ref-type=Figure S2 . " width="250" height="auto" />
    Anti Flag M2 F 3167, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag m2 f 3167/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti flag m2 f 3167 - by Bioz Stars, 2024-12
    86/100 stars

    Images

    1) Product Images from "Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP"

    Article Title: Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2018.10.024

    Hippo-Kinase-Independent YAP Serine Phosphorylation Determines Impaired Nuclear Translocation and Blunted YAP-Dependent Transcriptional Activity in Cav1KO Cells (A) Western blot of Ser112-phopshorylated YAP and total YAP in WT and Cav1KO MEFs and Cav1KO MEFs reconstituted with CAV1 (o_CAV1) or IRES-GFP. (B) Confocal immunofluorescence of cells transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with anti-FLAG antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). (C) FLAG distribution from analysis as in (B), represented as the ratio of nuclear-to-cytosolic intensities. n = 6–11. (D) Western blot analysis of FLAG subcellular distribution in MEFs transfected with YAP-FLAG or YAP(S5A)-FLAG followed by biochemical fractionation. RHO-GDI and Tef-1 were used as cytosolic and nuclear markers, respectively. The percentage of total YAP located in the nuclear fractions was quantified (right graph). (E) TEAD transcriptional activity in MEFs transfected with YAP-FLAG and YAP(S5A)-FLAG, measured by 8xGTICC-luciferase reporter assay. Data are normalized to growth on the soft substrate in each experiment. n = 3 (E). The fold-change with respect to untransfected cells is indicated above the bars. (F) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or Lats1 and 2 siRNAs. Data are normalized to WT control. n = 10. (G) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or NF2 siRNAs. Data are normalized to WT control. n = 4. Data are presented as mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, and ∗∗∗∗ p < 0.0005. See also <xref ref-type=Figure S2 . " title="... transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with anti-FLAG antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Hippo-Kinase-Independent YAP Serine Phosphorylation Determines Impaired Nuclear Translocation and Blunted YAP-Dependent Transcriptional Activity in Cav1KO Cells (A) Western blot of Ser112-phopshorylated YAP and total YAP in WT and Cav1KO MEFs and Cav1KO MEFs reconstituted with CAV1 (o_CAV1) or IRES-GFP. (B) Confocal immunofluorescence of cells transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with anti-FLAG antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). (C) FLAG distribution from analysis as in (B), represented as the ratio of nuclear-to-cytosolic intensities. n = 6–11. (D) Western blot analysis of FLAG subcellular distribution in MEFs transfected with YAP-FLAG or YAP(S5A)-FLAG followed by biochemical fractionation. RHO-GDI and Tef-1 were used as cytosolic and nuclear markers, respectively. The percentage of total YAP located in the nuclear fractions was quantified (right graph). (E) TEAD transcriptional activity in MEFs transfected with YAP-FLAG and YAP(S5A)-FLAG, measured by 8xGTICC-luciferase reporter assay. Data are normalized to growth on the soft substrate in each experiment. n = 3 (E). The fold-change with respect to untransfected cells is indicated above the bars. (F) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or Lats1 and 2 siRNAs. Data are normalized to WT control. n = 10. (G) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or NF2 siRNAs. Data are normalized to WT control. n = 4. Data are presented as mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, and ∗∗∗∗ p < 0.0005. See also Figure S2 .

    Techniques Used: Translocation Assay, Activity Assay, Western Blot, Immunofluorescence, Transfection, Staining, Fractionation, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing



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    anti flag m2 f 3167  (Millipore)
    86
    Millipore anti flag m2 f 3167
    Hippo-Kinase-Independent YAP Serine Phosphorylation Determines Impaired Nuclear Translocation and Blunted YAP-Dependent Transcriptional Activity in Cav1KO Cells (A) Western blot of Ser112-phopshorylated YAP and total YAP in WT and Cav1KO MEFs and Cav1KO MEFs reconstituted with CAV1 (o_CAV1) or IRES-GFP. (B) Confocal immunofluorescence of cells transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with <t>anti-FLAG</t> antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). (C) FLAG distribution from analysis as in (B), represented as the ratio of nuclear-to-cytosolic intensities. n = 6–11. (D) Western blot analysis of FLAG subcellular distribution in MEFs transfected with YAP-FLAG or YAP(S5A)-FLAG followed by biochemical fractionation. RHO-GDI and Tef-1 were used as cytosolic and nuclear markers, respectively. The percentage of total YAP located in the nuclear fractions was quantified (right graph). (E) TEAD transcriptional activity in MEFs transfected with YAP-FLAG and YAP(S5A)-FLAG, measured by 8xGTICC-luciferase reporter assay. Data are normalized to growth on the soft substrate in each experiment. n = 3 (E). The fold-change with respect to untransfected cells is indicated above the bars. (F) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or Lats1 and 2 siRNAs. Data are normalized to WT control. n = 10. (G) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or NF2 siRNAs. Data are normalized to WT control. n = 4. Data are presented as mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, and ∗∗∗∗ p < 0.0005. See also <xref ref-type=Figure S2 . " width="250" height="auto" />
    Anti Flag M2 F 3167, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag m2 f 3167/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti flag m2 f 3167 - by Bioz Stars, 2024-12
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Hippo-Kinase-Independent YAP Serine Phosphorylation Determines Impaired Nuclear Translocation and Blunted YAP-Dependent Transcriptional Activity in Cav1KO Cells (A) Western blot of Ser112-phopshorylated YAP and total YAP in WT and Cav1KO MEFs and Cav1KO MEFs reconstituted with CAV1 (o_CAV1) or IRES-GFP. (B) Confocal immunofluorescence of cells transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with anti-FLAG antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). (C) FLAG distribution from analysis as in (B), represented as the ratio of nuclear-to-cytosolic intensities. n = 6–11. (D) Western blot analysis of FLAG subcellular distribution in MEFs transfected with YAP-FLAG or YAP(S5A)-FLAG followed by biochemical fractionation. RHO-GDI and Tef-1 were used as cytosolic and nuclear markers, respectively. The percentage of total YAP located in the nuclear fractions was quantified (right graph). (E) TEAD transcriptional activity in MEFs transfected with YAP-FLAG and YAP(S5A)-FLAG, measured by 8xGTICC-luciferase reporter assay. Data are normalized to growth on the soft substrate in each experiment. n = 3 (E). The fold-change with respect to untransfected cells is indicated above the bars. (F) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or Lats1 and 2 siRNAs. Data are normalized to WT control. n = 10. (G) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or NF2 siRNAs. Data are normalized to WT control. n = 4. Data are presented as mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, and ∗∗∗∗ p < 0.0005. See also <xref ref-type=Figure S2 . " width="100%" height="100%">

    Journal: Cell Reports

    Article Title: Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP

    doi: 10.1016/j.celrep.2018.10.024

    Figure Lengend Snippet: Hippo-Kinase-Independent YAP Serine Phosphorylation Determines Impaired Nuclear Translocation and Blunted YAP-Dependent Transcriptional Activity in Cav1KO Cells (A) Western blot of Ser112-phopshorylated YAP and total YAP in WT and Cav1KO MEFs and Cav1KO MEFs reconstituted with CAV1 (o_CAV1) or IRES-GFP. (B) Confocal immunofluorescence of cells transfected with YAP-FLAG or YAP(S5A)-FLAG and stained with anti-FLAG antibody (green), fluorophore-conjugated phalloidin (red), and Hoechst (blue). (C) FLAG distribution from analysis as in (B), represented as the ratio of nuclear-to-cytosolic intensities. n = 6–11. (D) Western blot analysis of FLAG subcellular distribution in MEFs transfected with YAP-FLAG or YAP(S5A)-FLAG followed by biochemical fractionation. RHO-GDI and Tef-1 were used as cytosolic and nuclear markers, respectively. The percentage of total YAP located in the nuclear fractions was quantified (right graph). (E) TEAD transcriptional activity in MEFs transfected with YAP-FLAG and YAP(S5A)-FLAG, measured by 8xGTICC-luciferase reporter assay. Data are normalized to growth on the soft substrate in each experiment. n = 3 (E). The fold-change with respect to untransfected cells is indicated above the bars. (F) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or Lats1 and 2 siRNAs. Data are normalized to WT control. n = 10. (G) qRT-PCR for Ctgf and Ankrd1 expression in WT and CAV1 KO MEFs transfected with control or NF2 siRNAs. Data are normalized to WT control. n = 4. Data are presented as mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, and ∗∗∗∗ p < 0.0005. See also Figure S2 .

    Article Snippet: Monoclonal antibodies were sourced as follows: anti-YAP (sc-101199) and anti-TEF-1 (sc-376113) from Santa Cruz Biotechnology; anti-CAV1 XP (#3267) and anti-YWHAH (14-3-3 η (D23B7); #5521) from Cell Signaling (Danvers, Massachusetts, United States); anti-Flag M2 (F-3167) from Sigma-Aldrich; and anti-glyceraldehyde-3-phosphate dehydrogenase (MAB374) and anti-cortactin (p80/85, clone 4F11) from Millipore (Burlington, Massachusetts, United States).

    Techniques: Translocation Assay, Activity Assay, Western Blot, Immunofluorescence, Transfection, Staining, Fractionation, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing