Journal: Frontiers in Pharmacology
Article Title: Progressive iron overload in middle-aged mice impairs olfactory function, triggers lipid oxidation and induces apoptosis
doi: 10.3389/fphar.2024.1506944
Figure Lengend Snippet: Compensatory upregulation of iron metabolism and lipid oxidation pathways in response to iron overload in the olfactory bulb. (A–C) Immunohistochemical analysis of ferritin light chain FTL, (A) , transferrin receptor TFRC, (B) and ferroportin FPN, (C) in the olfactory bulb of NC (normal control) and FC (ferric citrate-treated) mice at 4, 8, 12, and 16 weeks. FC-treated mice show progressively increased expression of FTL, TFRC, and FPN over time, with more pronounced staining starting at week 8 and becoming markedly evident by week 16, suggesting a compensatory upregulation in response to iron overload. (D–F) Western blot analysis of FTL (D) , TFRC (E) and FPN (F) in the olfactory bulb of NC and FC mice at 4, 8, 12, and 16 weeks. In FC-treated mice, FTL levels are significantly elevated at weeks 12 and 16 (** P < 0.01; (D) ), TFRC levels increase significantly at week 16 (* P < 0.05) and are highly significant (** P < 0.01) at week 16 (E) , and FPN levels are significantly higher at week 12 (* P < 0.05) and week 16 (** P < 0.01; (F) ) compared to the NC group. (G–K) Quantitative PCR (qPCR) analysis of mRNA expression for FTL (G) , TFRC (H) , FPN (I) , SCL7A11 (J) and ACSL4 (K) in the olfactory bulb of NC and FC mice at 4, 8, 12, and 16 weeks. The FC group shows a significant increase in FTL mRNA at week 16 (** P < 0.01; (G) ), in TFRC mRNA levels from week 8 (** P < 0.01; (H) ), in FPN mRNA levels at week 12 (* P < 0.05) and week 16 (** P < 0.01; (I) ), in SCL7A11 mRNA levels from week 4 to week 16 (** P < 0.01 for all time points; (J) ), and in ACSL4 mRNA levels from week 4 onward (** P < 0.01; (K) ), indicating a robust and coordinated transcriptional response to iron overload and associated oxidative stress.
Article Snippet: Primary antibodies were applied against ferritin light chain (FTL, Genetex, GTX112943), ferroportin (SLC40A1, Proteintech, 26601-1-AP), and transferrin receptor (CD71, Proteintech, 10084-2-AP) and incubated overnight at 4°C.
Techniques: Immunohistochemical staining, Control, Expressing, Staining, Western Blot, Real-time Polymerase Chain Reaction
