anti ferret cd4  (Sino Biological)


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  • 90
    Name:
    CD4 Matched ELISA Antibody Pair Set Ferret
    Description:
    Each vial contains 140 ng of recombinant Ferret CD4 Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 3000 pg mL is recommended
    Catalog Number:
    SEK60003
    Price:
    None
    Category:
    Elisa pair set
    Reactivity:
    Ferret
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    Structured Review

    Sino Biological anti ferret cd4
    Each vial contains 140 ng of recombinant Ferret CD4 Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 3000 pg mL is recommended
    https://www.bioz.com/result/anti ferret cd4/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ferret cd4 - by Bioz Stars, 2021-05
    90/100 stars

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    Related Articles

    Positive Control:

    Article Title: Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
    Article Snippet: Data were analyzed using the system software and the result was presented as shifted wavelength (nm) at the end of the association step. .. To measure cell-mediated immunity (CMI), ferret peripheral blood leukocytes were stimulated overnight with positive control (PMA/ionomycin), negative control (allantoic fluid, or no stimulation), or with recombinant HA from Anhui/05, VN/04, or Pin/2014 virus, in the presence of Golgi-blocker for the last 6 h. The cells were then permeabilized, stained with anti-ferret CD4 (Sino Biological Inc, Beijing, China), anti-human CD8 (OKT8; eBioscience, Hatfield, UK), and anti-bovine interferon gamma (“IFNγ”) (MorphoSys, AbD Serotec, Oxford, UK), and analyzed by flow cytometry (Reber et al. submitted). ..

    Negative Control:

    Article Title: Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
    Article Snippet: Data were analyzed using the system software and the result was presented as shifted wavelength (nm) at the end of the association step. .. To measure cell-mediated immunity (CMI), ferret peripheral blood leukocytes were stimulated overnight with positive control (PMA/ionomycin), negative control (allantoic fluid, or no stimulation), or with recombinant HA from Anhui/05, VN/04, or Pin/2014 virus, in the presence of Golgi-blocker for the last 6 h. The cells were then permeabilized, stained with anti-ferret CD4 (Sino Biological Inc, Beijing, China), anti-human CD8 (OKT8; eBioscience, Hatfield, UK), and anti-bovine interferon gamma (“IFNγ”) (MorphoSys, AbD Serotec, Oxford, UK), and analyzed by flow cytometry (Reber et al. submitted). ..

    Recombinant:

    Article Title: Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
    Article Snippet: Data were analyzed using the system software and the result was presented as shifted wavelength (nm) at the end of the association step. .. To measure cell-mediated immunity (CMI), ferret peripheral blood leukocytes were stimulated overnight with positive control (PMA/ionomycin), negative control (allantoic fluid, or no stimulation), or with recombinant HA from Anhui/05, VN/04, or Pin/2014 virus, in the presence of Golgi-blocker for the last 6 h. The cells were then permeabilized, stained with anti-ferret CD4 (Sino Biological Inc, Beijing, China), anti-human CD8 (OKT8; eBioscience, Hatfield, UK), and anti-bovine interferon gamma (“IFNγ”) (MorphoSys, AbD Serotec, Oxford, UK), and analyzed by flow cytometry (Reber et al. submitted). ..

    Staining:

    Article Title: Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
    Article Snippet: Data were analyzed using the system software and the result was presented as shifted wavelength (nm) at the end of the association step. .. To measure cell-mediated immunity (CMI), ferret peripheral blood leukocytes were stimulated overnight with positive control (PMA/ionomycin), negative control (allantoic fluid, or no stimulation), or with recombinant HA from Anhui/05, VN/04, or Pin/2014 virus, in the presence of Golgi-blocker for the last 6 h. The cells were then permeabilized, stained with anti-ferret CD4 (Sino Biological Inc, Beijing, China), anti-human CD8 (OKT8; eBioscience, Hatfield, UK), and anti-bovine interferon gamma (“IFNγ”) (MorphoSys, AbD Serotec, Oxford, UK), and analyzed by flow cytometry (Reber et al. submitted). ..

    Flow Cytometry:

    Article Title: Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
    Article Snippet: Data were analyzed using the system software and the result was presented as shifted wavelength (nm) at the end of the association step. .. To measure cell-mediated immunity (CMI), ferret peripheral blood leukocytes were stimulated overnight with positive control (PMA/ionomycin), negative control (allantoic fluid, or no stimulation), or with recombinant HA from Anhui/05, VN/04, or Pin/2014 virus, in the presence of Golgi-blocker for the last 6 h. The cells were then permeabilized, stained with anti-ferret CD4 (Sino Biological Inc, Beijing, China), anti-human CD8 (OKT8; eBioscience, Hatfield, UK), and anti-bovine interferon gamma (“IFNγ”) (MorphoSys, AbD Serotec, Oxford, UK), and analyzed by flow cytometry (Reber et al. submitted). ..

    Cytometry:

    Article Title: Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
    Article Snippet: Data were analyzed using the system software and the result was presented as shifted wavelength (nm) at the end of the association step. .. To measure cell-mediated immunity (CMI), ferret peripheral blood leukocytes were stimulated overnight with positive control (PMA/ionomycin), negative control (allantoic fluid, or no stimulation), or with recombinant HA from Anhui/05, VN/04, or Pin/2014 virus, in the presence of Golgi-blocker for the last 6 h. The cells were then permeabilized, stained with anti-ferret CD4 (Sino Biological Inc, Beijing, China), anti-human CD8 (OKT8; eBioscience, Hatfield, UK), and anti-bovine interferon gamma (“IFNγ”) (MorphoSys, AbD Serotec, Oxford, UK), and analyzed by flow cytometry (Reber et al. submitted). ..

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  • 93
    Sino Biological cd4
    Changes in peripheral blood leukocyte counts following influenza challenge. a On days 1–7, 9, 11, and 13 following intranasal challenge with cell-grown HK/4801, blood samples were drawn and peripheral blood leukocytes were analyzed using flow cytometry as described in the Methods section. Mean percent of PBLs comprising <t>CD4+</t> T cells (“CD4”), CD8+ T cells (“CD8”), or CD11b+ granulocytes (“granulocytes”) are shown as the mean of each group, with values normalized to values on the day of infection (day 0). Error bars represent one standard deviation; six ferrets per group until day 2 then three ferrets per group. b As above, with replicates combined. c Statistical significances of the differences between groups, as measured using a linear mixed model with repeated measures
    Cd4, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd4 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    91
    Sino Biological anti mouse cd4 monoclonal antibody
    Frequencies of the tumor infiltrated immune cells Frequencies of <t>CD4</t> + and CD8 + T cells, CD11b + Ly6G + (PMN-MDSC), CD11c + (DC) and CD11b + Ly6C + (M-MDSC) cells among the tumor infiltration immune cells of mice treated with the WT1 CTL peptide vaccine alone (open bar, n = 8) or the combination vaccine with the WT1 CTL and helper peptides (solid bar, n = 8) are shown. Bar graphs show averages and standard deviations.
    Anti Mouse Cd4 Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse cd4 monoclonal antibody/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse cd4 monoclonal antibody - by Bioz Stars, 2021-05
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    92
    Sino Biological cd4 antibody pe mouse mab
    <t>CD4+</t> T cell responses to influenza peptide pools. Splenocytes were collected from naïve ferrets infected intranasally with A/NY/21/2009 (Naïve/H1N1pdm09, n = 4), A/Perth/16/2009 (Naïve/H3N2, n = 4), or mock infected ferrets (Naïve/Mock, n = 4) 14d post infection. Splenocytes were also collected from ferrets vaccinated with commercial 2011–2012 TIV, challenged with A/NY/21/2009 (TIV/H1N1pdm09, n = 4). Splenocytes were stimulated with peptide pools (p) derived from regions of each influenza protein from A/California/07/2009 (H1N1pdm09; ( A ) or the A/Perth/16/2009-like seasonal H3N2 strain, A/Victoria/210/2009 ( B ). T cells producing IFN-γ in response to peptide stimulation were assessed by flow cytometry. The percent IFN-γ+ cells and fold-increase over mock infected animals are depicted. Responses significantly 1 greater (p
    Cd4 Antibody Pe Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4 antibody pe mouse mab/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd4 antibody pe mouse mab - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Changes in peripheral blood leukocyte counts following influenza challenge. a On days 1–7, 9, 11, and 13 following intranasal challenge with cell-grown HK/4801, blood samples were drawn and peripheral blood leukocytes were analyzed using flow cytometry as described in the Methods section. Mean percent of PBLs comprising CD4+ T cells (“CD4”), CD8+ T cells (“CD8”), or CD11b+ granulocytes (“granulocytes”) are shown as the mean of each group, with values normalized to values on the day of infection (day 0). Error bars represent one standard deviation; six ferrets per group until day 2 then three ferrets per group. b As above, with replicates combined. c Statistical significances of the differences between groups, as measured using a linear mixed model with repeated measures

    Journal: NPJ Vaccines

    Article Title: Repeated vaccination against matched H3N2 influenza virus gives less protection than single vaccination in ferrets

    doi: 10.1038/s41541-019-0123-7

    Figure Lengend Snippet: Changes in peripheral blood leukocyte counts following influenza challenge. a On days 1–7, 9, 11, and 13 following intranasal challenge with cell-grown HK/4801, blood samples were drawn and peripheral blood leukocytes were analyzed using flow cytometry as described in the Methods section. Mean percent of PBLs comprising CD4+ T cells (“CD4”), CD8+ T cells (“CD8”), or CD11b+ granulocytes (“granulocytes”) are shown as the mean of each group, with values normalized to values on the day of infection (day 0). Error bars represent one standard deviation; six ferrets per group until day 2 then three ferrets per group. b As above, with replicates combined. c Statistical significances of the differences between groups, as measured using a linear mixed model with repeated measures

    Article Snippet: Peripheral blood leukocyte (PBL) purification and flow cytometry were performed (Music et al. , Music et al. ), using monoclonal antibodies recognizing ferret CD4 (Sino Biological Inc., Beijing, China), or cross-reacting with ferret CD8 (eBioscience, San Diego, CA) or CD11b (clone M1/70, eBioscience, 1:20 dilution).

    Techniques: Flow Cytometry, Infection, Standard Deviation

    Frequencies of the tumor infiltrated immune cells Frequencies of CD4 + and CD8 + T cells, CD11b + Ly6G + (PMN-MDSC), CD11c + (DC) and CD11b + Ly6C + (M-MDSC) cells among the tumor infiltration immune cells of mice treated with the WT1 CTL peptide vaccine alone (open bar, n = 8) or the combination vaccine with the WT1 CTL and helper peptides (solid bar, n = 8) are shown. Bar graphs show averages and standard deviations.

    Journal: Oncotarget

    Article Title: Extremely strong infiltration of WT1-specific CTLs into mouse tumor by the combination vaccine with WT1-specific CTL and helper peptides

    doi: 10.18632/oncotarget.26338

    Figure Lengend Snippet: Frequencies of the tumor infiltrated immune cells Frequencies of CD4 + and CD8 + T cells, CD11b + Ly6G + (PMN-MDSC), CD11c + (DC) and CD11b + Ly6C + (M-MDSC) cells among the tumor infiltration immune cells of mice treated with the WT1 CTL peptide vaccine alone (open bar, n = 8) or the combination vaccine with the WT1 CTL and helper peptides (solid bar, n = 8) are shown. Bar graphs show averages and standard deviations.

    Article Snippet: After the peroxidase-blocking with Dako peroxidase-blocking solution (DAKO), the sections were incubated with anti-mouse CD4 monoclonal antibody (Sino Biological, Beijing, China) or anti-mouse CD8 polyclonal antibody (Bioss Antibodies, Boston, MA, USA) or Anti-ITGAX rabbit polyclonal antibody (OriGene Technologies, Rockville, MD, USA) overnight.

    Techniques: Mouse Assay

    Tumor infiltrated WT1 tetramer + CD8 + T cells ( A ) Bee swarm plots of the frequencies of WT1-tetramer + CD8 + T cells among CD8 + T cells in tumors of the mice treated with the WT1 CTL peptide vaccine alone or the combination vaccine with the WT1 CTL and helper peptides. ( B ) Bee swarm plots of the number of WT1-tetramer + CD8 + T cells per 1 × 10 5 tumor cells. ( C ) A representative result of flow cytometry showing the high frequencies of WT1-tetramer + CD8 + T cells among CD8 + T cells in tumors of the mice treated with the combination vaccine. ( D ) Representative results of flow cytometry of cytokine releasing assay of tumor infiltrating CD4 + T cells in mice treated with the combination vaccine. Splenocytes from a CD45.2 mouse pulsed or not pulsed with the WT1 helper peptide were used as the stimulator and control cells, respectively. The results of intracellular staining of IFNγ and TNFα of CD3 + CD4 + CD45.1 + T cells are shown.

    Journal: Oncotarget

    Article Title: Extremely strong infiltration of WT1-specific CTLs into mouse tumor by the combination vaccine with WT1-specific CTL and helper peptides

    doi: 10.18632/oncotarget.26338

    Figure Lengend Snippet: Tumor infiltrated WT1 tetramer + CD8 + T cells ( A ) Bee swarm plots of the frequencies of WT1-tetramer + CD8 + T cells among CD8 + T cells in tumors of the mice treated with the WT1 CTL peptide vaccine alone or the combination vaccine with the WT1 CTL and helper peptides. ( B ) Bee swarm plots of the number of WT1-tetramer + CD8 + T cells per 1 × 10 5 tumor cells. ( C ) A representative result of flow cytometry showing the high frequencies of WT1-tetramer + CD8 + T cells among CD8 + T cells in tumors of the mice treated with the combination vaccine. ( D ) Representative results of flow cytometry of cytokine releasing assay of tumor infiltrating CD4 + T cells in mice treated with the combination vaccine. Splenocytes from a CD45.2 mouse pulsed or not pulsed with the WT1 helper peptide were used as the stimulator and control cells, respectively. The results of intracellular staining of IFNγ and TNFα of CD3 + CD4 + CD45.1 + T cells are shown.

    Article Snippet: After the peroxidase-blocking with Dako peroxidase-blocking solution (DAKO), the sections were incubated with anti-mouse CD4 monoclonal antibody (Sino Biological, Beijing, China) or anti-mouse CD8 polyclonal antibody (Bioss Antibodies, Boston, MA, USA) or Anti-ITGAX rabbit polyclonal antibody (OriGene Technologies, Rockville, MD, USA) overnight.

    Techniques: Mouse Assay, Flow Cytometry, Staining

    Fingolimod significantly reduces CNS infiltration. (A) Coronal sections of brains from fingolimod treated mice and controls were stained with H E, and the choroid plexus was blindly scored for lymphocytic infiltration (10x magnification). Representative sections are shown. (B) Immunofluorescent staining of CD4+ T cells (magenta) and CD8+ T cells (green) and quantification of cells per field at 20x magnification. (C) Iba1+ macrophages (magenta) were also quantified at 20x magnification. Control treated, n = 7; Fingolimod treated, n = 8. (D) Quantification of infiltrating T cells, macrophages, eosinophils, and natural killer cells by flow cytometry from hemisected brain lysates. Control treated, n = 10; Fingolimod treated, n = 7. * p

    Journal: Frontiers in Immunology

    Article Title: Neuropsychiatric Systemic Lupus Erythematosus Is Dependent on Sphingosine-1-Phosphate Signaling

    doi: 10.3389/fimmu.2018.02189

    Figure Lengend Snippet: Fingolimod significantly reduces CNS infiltration. (A) Coronal sections of brains from fingolimod treated mice and controls were stained with H E, and the choroid plexus was blindly scored for lymphocytic infiltration (10x magnification). Representative sections are shown. (B) Immunofluorescent staining of CD4+ T cells (magenta) and CD8+ T cells (green) and quantification of cells per field at 20x magnification. (C) Iba1+ macrophages (magenta) were also quantified at 20x magnification. Control treated, n = 7; Fingolimod treated, n = 8. (D) Quantification of infiltrating T cells, macrophages, eosinophils, and natural killer cells by flow cytometry from hemisected brain lysates. Control treated, n = 10; Fingolimod treated, n = 7. * p

    Article Snippet: Staining for Iba-1 (rabbit anti-Iba-1, Wako, Osaka, Japan), IgG (donkey anti-mouse IgG, Jackson Immunoresearch Laboratories, West Grove, PA), CD3 (rabbit anti-mouse CD3, ThermoFisher Scientific), CD4 (rabbit anti-mouse CD4, Sino Biological, Wayne, PA), CD8 (rat anti-mouse CD8, eBioscence, San Diego, CA), B220 (rat anti-mouse B220, BD Biosciences, San Jose, CA), and albumin (goat anti-mouse albumin, Bethyl Laboratories, Montgomery, TX), were performed on 5 μm coronal sections.

    Techniques: Mouse Assay, Staining, Flow Cytometry

    CD4+ T cell responses to influenza peptide pools. Splenocytes were collected from naïve ferrets infected intranasally with A/NY/21/2009 (Naïve/H1N1pdm09, n = 4), A/Perth/16/2009 (Naïve/H3N2, n = 4), or mock infected ferrets (Naïve/Mock, n = 4) 14d post infection. Splenocytes were also collected from ferrets vaccinated with commercial 2011–2012 TIV, challenged with A/NY/21/2009 (TIV/H1N1pdm09, n = 4). Splenocytes were stimulated with peptide pools (p) derived from regions of each influenza protein from A/California/07/2009 (H1N1pdm09; ( A ) or the A/Perth/16/2009-like seasonal H3N2 strain, A/Victoria/210/2009 ( B ). T cells producing IFN-γ in response to peptide stimulation were assessed by flow cytometry. The percent IFN-γ+ cells and fold-increase over mock infected animals are depicted. Responses significantly 1 greater (p

    Journal: Scientific Reports

    Article Title: Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model

    doi: 10.1038/s41598-018-24394-z

    Figure Lengend Snippet: CD4+ T cell responses to influenza peptide pools. Splenocytes were collected from naïve ferrets infected intranasally with A/NY/21/2009 (Naïve/H1N1pdm09, n = 4), A/Perth/16/2009 (Naïve/H3N2, n = 4), or mock infected ferrets (Naïve/Mock, n = 4) 14d post infection. Splenocytes were also collected from ferrets vaccinated with commercial 2011–2012 TIV, challenged with A/NY/21/2009 (TIV/H1N1pdm09, n = 4). Splenocytes were stimulated with peptide pools (p) derived from regions of each influenza protein from A/California/07/2009 (H1N1pdm09; ( A ) or the A/Perth/16/2009-like seasonal H3N2 strain, A/Victoria/210/2009 ( B ). T cells producing IFN-γ in response to peptide stimulation were assessed by flow cytometry. The percent IFN-γ+ cells and fold-increase over mock infected animals are depicted. Responses significantly 1 greater (p

    Article Snippet: Cells were stained with a live/dead stain (Life Technologies, Grand Island, NY), then stained with monoclonal antibodies recognizing CD4 (60003-MM02-P, clone: 02, Sino Biological, Beijing, China), CD8 (48-0086-42, clone: OKT-8, eBioscience, San Diego, CA), and IFN-γ (MCA1783A647, clone: CC302, AbD Serotec, Raleigh, NC) , and analyzed using a Canto II Flow Cytometer (BD Biosciences).

    Techniques: Infection, Derivative Assay, Flow Cytometry

    Longevity of the cross-reactive CD4 T cell response. Naïve ferrets were infected intranasally with H1N1pdm09 strain, A/NY/21/2009 (H1N1pdm09, n = 7), or were mock infected (Mock; n = 6). Peripheral blood leukocytes were purified weekly for 6 weeks, and T cell responses assessed by stimulation with a panel of live influenza strains ( A ). Splenocytes were collected 6 weeks post-infection and stimulated with the panel of live influenza strains ( B ). Responding CD4 T cells were assessed for IFN-γ production by flow cytometry. Significance from the mock infected group is indicated by *p

    Journal: Scientific Reports

    Article Title: Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model

    doi: 10.1038/s41598-018-24394-z

    Figure Lengend Snippet: Longevity of the cross-reactive CD4 T cell response. Naïve ferrets were infected intranasally with H1N1pdm09 strain, A/NY/21/2009 (H1N1pdm09, n = 7), or were mock infected (Mock; n = 6). Peripheral blood leukocytes were purified weekly for 6 weeks, and T cell responses assessed by stimulation with a panel of live influenza strains ( A ). Splenocytes were collected 6 weeks post-infection and stimulated with the panel of live influenza strains ( B ). Responding CD4 T cells were assessed for IFN-γ production by flow cytometry. Significance from the mock infected group is indicated by *p

    Article Snippet: Cells were stained with a live/dead stain (Life Technologies, Grand Island, NY), then stained with monoclonal antibodies recognizing CD4 (60003-MM02-P, clone: 02, Sino Biological, Beijing, China), CD8 (48-0086-42, clone: OKT-8, eBioscience, San Diego, CA), and IFN-γ (MCA1783A647, clone: CC302, AbD Serotec, Raleigh, NC) , and analyzed using a Canto II Flow Cytometer (BD Biosciences).

    Techniques: Infection, Purification, Flow Cytometry

    Extensive T cell cross-reactivity between a diverse selection of influenza strains. Naïve ferrets were infected intranasally with H1N1pdm09 strain, A/NY/21/2009 (Naïve/H1N1pdm09, n = 8), the seasonal H3N2 strain, A/Perth/16/2009 (Naïve/H3N2, n = 4), or were mock infected (Naïve/Mock, n = 8). An additional group of ferrets was vaccinated with a commercially available 2011–2012 trivalent inactivated influenza vaccine prior to challenge with A/NY/21/2009 (TIV/H1N1pdm09, n = 8). Splenocytes were collected from ferrets 14d post-infection and stimulated with a diverse array of contemporary and historical influenza strains from each of the currently circulating influenza subtypes. IFN-γ production was assessed by flow cytometry for CD4+ ( A ) and CD8+ ( B ) T cell populations. Significance from the mock infected group is indicated by *p

    Journal: Scientific Reports

    Article Title: Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model

    doi: 10.1038/s41598-018-24394-z

    Figure Lengend Snippet: Extensive T cell cross-reactivity between a diverse selection of influenza strains. Naïve ferrets were infected intranasally with H1N1pdm09 strain, A/NY/21/2009 (Naïve/H1N1pdm09, n = 8), the seasonal H3N2 strain, A/Perth/16/2009 (Naïve/H3N2, n = 4), or were mock infected (Naïve/Mock, n = 8). An additional group of ferrets was vaccinated with a commercially available 2011–2012 trivalent inactivated influenza vaccine prior to challenge with A/NY/21/2009 (TIV/H1N1pdm09, n = 8). Splenocytes were collected from ferrets 14d post-infection and stimulated with a diverse array of contemporary and historical influenza strains from each of the currently circulating influenza subtypes. IFN-γ production was assessed by flow cytometry for CD4+ ( A ) and CD8+ ( B ) T cell populations. Significance from the mock infected group is indicated by *p

    Article Snippet: Cells were stained with a live/dead stain (Life Technologies, Grand Island, NY), then stained with monoclonal antibodies recognizing CD4 (60003-MM02-P, clone: 02, Sino Biological, Beijing, China), CD8 (48-0086-42, clone: OKT-8, eBioscience, San Diego, CA), and IFN-γ (MCA1783A647, clone: CC302, AbD Serotec, Raleigh, NC) , and analyzed using a Canto II Flow Cytometer (BD Biosciences).

    Techniques: Selection, Infection, Flow Cytometry