anti fascin  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Anti Fascin antibody
    Description:

    Catalog Number:
    ab183891
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam anti fascin
    NF-κB binds to the <t>fascin</t> promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB <t>p50</t> siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P

    https://www.bioz.com/result/anti fascin/product/Abcam
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti fascin - by Bioz Stars, 2020-07
    93/100 stars

    Images

    1) Product Images from "Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration"

    Article Title: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration

    Journal: Oncology Letters

    doi: 10.3892/ol.2014.1804

    NF-κB binds to the fascin promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB p50 siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P
    Figure Legend Snippet: NF-κB binds to the fascin promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB p50 siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P

    Techniques Used: Chromatin Immunoprecipitation, Binding Assay, Transfection, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Standard Deviation

    2) Product Images from "Proteomic Analysis of Exosomes Secreted by Human Mesothelioma Cells"

    Article Title: Proteomic Analysis of Exosomes Secreted by Human Mesothelioma Cells

    Journal: The American Journal of Pathology

    doi:

    The presence of fascin (I), β-tubulin ( II ), HSC70 ( III ), and HSP90 ( IV ) were confirmed by Western blots of PMR-MM7-derived exosomes ( A ) and PMR-MM8-derived exosomes (B). The primary antibodies anti-fascin (1:1000), anti-β-tubulin (1:10.000), and anti-HSP90 (1:2000) were followed by horseradish peroxidase-conjugated goat anti-mouse IgG1. Anti-HSC70 (1:1000) was followed by Envision (DAKO). Blots were incubated with SuperSignal West Pico chemiluminescent substrate and exposed to Hyperfilm ECL (Amersham Biosciences, Buckinghamshire, England).
    Figure Legend Snippet: The presence of fascin (I), β-tubulin ( II ), HSC70 ( III ), and HSP90 ( IV ) were confirmed by Western blots of PMR-MM7-derived exosomes ( A ) and PMR-MM8-derived exosomes (B). The primary antibodies anti-fascin (1:1000), anti-β-tubulin (1:10.000), and anti-HSP90 (1:2000) were followed by horseradish peroxidase-conjugated goat anti-mouse IgG1. Anti-HSC70 (1:1000) was followed by Envision (DAKO). Blots were incubated with SuperSignal West Pico chemiluminescent substrate and exposed to Hyperfilm ECL (Amersham Biosciences, Buckinghamshire, England).

    Techniques Used: Western Blot, Derivative Assay, Incubation

    3) Product Images from "Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration"

    Article Title: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration

    Journal: Oncology Letters

    doi: 10.3892/ol.2014.1804

    NF-κB binds to the fascin promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB p50 siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P
    Figure Legend Snippet: NF-κB binds to the fascin promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB p50 siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P

    Techniques Used: Chromatin Immunoprecipitation, Binding Assay, Transfection, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Standard Deviation

    STAT3 is required for IL-6-induced expression of fascin and cell migration in MKN45 cells. (A) MKN45 cells were transfected with STAT3 siRNA or treated with AG490 and cultured for 24 h. Whole cell extracts were subjected to western blotting with antibodies against pSTAT3, total STAT3, MMP-2 and -9 and GAPDH. MKN45 cells were treated with IL-6 for 30 min. (B) MKN45 cells were transfected with STAT3 siRNA or treated with AG490 and cultured for 3 days prior to treatment with IL-6 for 30 min. mRNA levels of fascin were detected by quantitative polymerase chain reaction. Results were standardized to GAPDH and expressed as fold induction of IL-6-treated cells from three independent experiments. (C) MKN45 cells were treated as described and wound healing assays were performed. The percentage of the wound occupied in three independent experiments was calculated. (D) MKN45 cells were treated as described and cell migration assays were performed. The mean number of migrated cells in at least 5 visual fields of 3 independent experiments was calculated. (E) MKN45 cells were treated as described and cell invasion assays were performed. Invaded cells were stained and eluted for absorbance readings at 560 nm. (F) Representative experiments of wound healing assays are shown (magnification, ×100). (G) Representative experiments of cell migration assays are shown (magnification, ×100). * P
    Figure Legend Snippet: STAT3 is required for IL-6-induced expression of fascin and cell migration in MKN45 cells. (A) MKN45 cells were transfected with STAT3 siRNA or treated with AG490 and cultured for 24 h. Whole cell extracts were subjected to western blotting with antibodies against pSTAT3, total STAT3, MMP-2 and -9 and GAPDH. MKN45 cells were treated with IL-6 for 30 min. (B) MKN45 cells were transfected with STAT3 siRNA or treated with AG490 and cultured for 3 days prior to treatment with IL-6 for 30 min. mRNA levels of fascin were detected by quantitative polymerase chain reaction. Results were standardized to GAPDH and expressed as fold induction of IL-6-treated cells from three independent experiments. (C) MKN45 cells were treated as described and wound healing assays were performed. The percentage of the wound occupied in three independent experiments was calculated. (D) MKN45 cells were treated as described and cell migration assays were performed. The mean number of migrated cells in at least 5 visual fields of 3 independent experiments was calculated. (E) MKN45 cells were treated as described and cell invasion assays were performed. Invaded cells were stained and eluted for absorbance readings at 560 nm. (F) Representative experiments of wound healing assays are shown (magnification, ×100). (G) Representative experiments of cell migration assays are shown (magnification, ×100). * P

    Techniques Used: Expressing, Migration, Transfection, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Staining

    Fascin is directly regulated by STAT3 in response to IL-6 in MKN45 cells. (A) Western blotting was performed to assess protein levels of fascin, Notch1 and Notch2 in MKN45 cells treated with 50 μM AG490 for 24–48 h. (B) Cells were incubated with IL-6 and/or AG490/DAPT and western blotting was performed. (C) Cells were incubated with IL-6 and ChIP assays were performed with STAT3 antibody and primers flanking a potential STAT3 site in the human fascin promoter. (D) Results from (C) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (E) The Hes-1 and fascin expression levels in MKN45 cells treated with DAPT were assessed by western blotting. Results represent three independent experiments performed in triplicate. ** P
    Figure Legend Snippet: Fascin is directly regulated by STAT3 in response to IL-6 in MKN45 cells. (A) Western blotting was performed to assess protein levels of fascin, Notch1 and Notch2 in MKN45 cells treated with 50 μM AG490 for 24–48 h. (B) Cells were incubated with IL-6 and/or AG490/DAPT and western blotting was performed. (C) Cells were incubated with IL-6 and ChIP assays were performed with STAT3 antibody and primers flanking a potential STAT3 site in the human fascin promoter. (D) Results from (C) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (E) The Hes-1 and fascin expression levels in MKN45 cells treated with DAPT were assessed by western blotting. Results represent three independent experiments performed in triplicate. ** P

    Techniques Used: Western Blot, Incubation, Chromatin Immunoprecipitation, Expressing

    Fascin and STAT3 are upregulated by IL-6 in MKN45 cells. (A) Fascin expression of five GC cell lines and the GES-1 cell line was determined by western blotting. (B) MKN45 cells were treated with IL-6 for 1 h and the expression levels of fascin, total STAT3 and pSTAT3 (Tyr705) were detected using western blotting. (C) Quantitative polymerase chain reaction analysis measured fascin mRNA levels in MKN45 cells treated with IL-6 for 1, 2, 3 or 4 h. Results were standardized to GAPDH and shown relative to the untreated sample. Results represent three independent experiments performed in triplicate. STAT3, signal transducer and activator of transcription 3; pSTAT3, phosphotyrosine STAT3; IL-6, interleukin-6; GC, gastric carcinoma. * P
    Figure Legend Snippet: Fascin and STAT3 are upregulated by IL-6 in MKN45 cells. (A) Fascin expression of five GC cell lines and the GES-1 cell line was determined by western blotting. (B) MKN45 cells were treated with IL-6 for 1 h and the expression levels of fascin, total STAT3 and pSTAT3 (Tyr705) were detected using western blotting. (C) Quantitative polymerase chain reaction analysis measured fascin mRNA levels in MKN45 cells treated with IL-6 for 1, 2, 3 or 4 h. Results were standardized to GAPDH and shown relative to the untreated sample. Results represent three independent experiments performed in triplicate. STAT3, signal transducer and activator of transcription 3; pSTAT3, phosphotyrosine STAT3; IL-6, interleukin-6; GC, gastric carcinoma. * P

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Related Articles

    Blocking Assay:

    Article Title: Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells
    Article Snippet: .. After blocking for 2 h with phosphate-buffered saline containing 0.1% Tween 20 (PBST) and 5% powdered skim milk, the blots were incubated with primary Notch4 (cat. no. ab134831; 1:1,000), p-Akt (cat. no. ab8933; 1:500), Akt (cat. no. ab8805; 1:1,000), GSK3 (cat. no. ab131344; 1:500), p-GSK3 (cat. no. ab28808; 1:500) and fascin (cat. no. ab97753; 1:1,000) antibodies (Abcam, Cambridge, UK) overnight in 5% powdered skim milk buffer, washed thrice with PBST and incubated with horseradish peroxidase-conjugated anti-rabbit (cat. no. 7074) and anti-mouse (cat. no. 7076) IgG secondary antibodies (1:2,000; Cell Signaling Technology, Beverly, MA, USA). .. An anti-glyceraldehyde 3-phosphate dehydrogenase antibody (cat. no. sc-25778; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) was used as a control.

    Electrophoresis:

    Article Title: Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway
    Article Snippet: .. Proteins were separated by electrophoresis on a 12% SDS-polyacrylamide gel, electroblotted onto a PVDF membrane (BioRad Laboratories), and incubated with anti-Bcl-xL, anti-caspase-3, anti-caspase-7, anti-caspase-9, anti-STAT3, anti-vimentin, anti-p-STAT3, anti-β-actin, anti-Histone H3 (Cell Signaling Technology), anti-Bcl-2, anti-Bax, anti-Fascin (Abcam), anti-PARP1, anti-MMP2, and anti-MMP9 (Proteintech). .. Immunoreactivity was determined by using a Chemi DOC™ XRS+ system (BioRad Laboratories).

    Incubation:

    Article Title: Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway
    Article Snippet: .. Proteins were separated by electrophoresis on a 12% SDS-polyacrylamide gel, electroblotted onto a PVDF membrane (BioRad Laboratories), and incubated with anti-Bcl-xL, anti-caspase-3, anti-caspase-7, anti-caspase-9, anti-STAT3, anti-vimentin, anti-p-STAT3, anti-β-actin, anti-Histone H3 (Cell Signaling Technology), anti-Bcl-2, anti-Bax, anti-Fascin (Abcam), anti-PARP1, anti-MMP2, and anti-MMP9 (Proteintech). .. Immunoreactivity was determined by using a Chemi DOC™ XRS+ system (BioRad Laboratories).

    Article Title: Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells
    Article Snippet: .. After blocking for 2 h with phosphate-buffered saline containing 0.1% Tween 20 (PBST) and 5% powdered skim milk, the blots were incubated with primary Notch4 (cat. no. ab134831; 1:1,000), p-Akt (cat. no. ab8933; 1:500), Akt (cat. no. ab8805; 1:1,000), GSK3 (cat. no. ab131344; 1:500), p-GSK3 (cat. no. ab28808; 1:500) and fascin (cat. no. ab97753; 1:1,000) antibodies (Abcam, Cambridge, UK) overnight in 5% powdered skim milk buffer, washed thrice with PBST and incubated with horseradish peroxidase-conjugated anti-rabbit (cat. no. 7074) and anti-mouse (cat. no. 7076) IgG secondary antibodies (1:2,000; Cell Signaling Technology, Beverly, MA, USA). .. An anti-glyceraldehyde 3-phosphate dehydrogenase antibody (cat. no. sc-25778; 1:1,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) was used as a control.

    Article Title: Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents
    Article Snippet: .. The gel was sliced and transferred onto the polyvinylidene fluoride membrane, which was initially treated with the closure solution at room temperature and agitated for 1 h. The primary antibodies [rabbit anti-human polyclonal antibodies against ZNF139 (cat. no. ab126124), pyridoxal kinase (PDXK; cat. no. ab38208), annexin A2 (ANXA2; cat. no. ab41803) and fascin (cat. no. ab183891), and mouse anti-human monoclonal antibody against β-actin (cat. no. ab6276); Abcam, Cambridge, UK] were added and then incubated at 4°C overnight. .. The membrane was washed, followed by the addition of horseradish peroxidase enzyme-labelled goat anti-rabbit IgG secondary antibody (cat. no. ab6721; Abcam) and incubated at room temperature for 1 h. The membrane was washed again and stained by chemiluminescence.

    other:

    Article Title: p53 controls colorectal cancer cell invasion by inhibiting the NF-κB-mediated activation of Fascin
    Article Snippet: Fascin antibody (ab78599) was obtained from Abcam.

    Article Title: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration
    Article Snippet: Anti-fascin, anti-activated-Notch1, anti-activated-Notch2, anti-Hes-1, anti-STAT3, anti-pSTAT3, anti-MMP-2 and -9, anti-NF-κB p50 and anti-GAPDH antibodies were used.

    IA:

    Article Title: Proteomic Analysis of Exosomes Secreted by Human Mesothelioma Cells
    Article Snippet: .. Antibodies used in this study to confirm the proteins detected by MALDI-TOF were: anti-HSC70 (clone 13D3; Affinity BioReagents, Golden, CO), anti-HSP90 (clone AC88; Stressgen, Victoria, Canada), anti-fascin (clone FCN01, Abcam Ltd, Cambridge, UK), and anti-β-tubulin (clone E7, Developmental Studies Hybridoma Bank, Iowa City, IA). ..

    Western Blot:

    Article Title: Daam1 regulates fascin for actin assembly in mouse oocyte meiosis
    Article Snippet: .. Mouse monoclonal anti-Daam1 antibody (for Western blot) and rabbit monoclonal anti-fascin antibody were purchased from Abcam (Cambridge, UK). α-tubulin (11H10) Rabbit mAb and HRP-conjugated anti-mouse IgG were from Cell Signaling Technology (Beverly, MA, USA). .. HRP-conjugated goat anti-rabbit IgG was bought from Vazyme (Nanjing, China).

    Article Title: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration
    Article Snippet: .. Antibodies used for western blotting and chromatin immunoprecipitation (ChIP) assays were anti-phosphotyrosine (p)STAT3 (Cell Signaling Technology, Inc., Beverly, MA, USA), anti-STAT3 (Cell Signaling Technology, Inc.), anti-nuclear factor (NF)-κB p50 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-fascin (Abcam, Cambridge, UK), anti-hairy and enhancer of split-1 (Hes-1; Abcam), anti-activated-Notch1 (Abcam), anti-activated-Notch2 (Abcam), anti-matrix metalloproteinase (MMP)-2 and anti-MMP-9 (Cell Signaling Technology, Inc.), anti-GAPDH (Abcam), anti-rabbit immunoglobulin (Ig)G and horseradish peroxidase-linked antibody (Cell Signaling Technology, Inc.). .. ChIP assays ChIP assays were performed using a ChIP assay kit, according to the manufacturer’s instructions (Upstate Biotechnology, Lake Placid, NY, USA) as described previously ( , ).

    Chromatin Immunoprecipitation:

    Article Title: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration
    Article Snippet: .. Antibodies used for western blotting and chromatin immunoprecipitation (ChIP) assays were anti-phosphotyrosine (p)STAT3 (Cell Signaling Technology, Inc., Beverly, MA, USA), anti-STAT3 (Cell Signaling Technology, Inc.), anti-nuclear factor (NF)-κB p50 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-fascin (Abcam, Cambridge, UK), anti-hairy and enhancer of split-1 (Hes-1; Abcam), anti-activated-Notch1 (Abcam), anti-activated-Notch2 (Abcam), anti-matrix metalloproteinase (MMP)-2 and anti-MMP-9 (Cell Signaling Technology, Inc.), anti-GAPDH (Abcam), anti-rabbit immunoglobulin (Ig)G and horseradish peroxidase-linked antibody (Cell Signaling Technology, Inc.). .. ChIP assays ChIP assays were performed using a ChIP assay kit, according to the manufacturer’s instructions (Upstate Biotechnology, Lake Placid, NY, USA) as described previously ( , ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Abcam anti fascin
    NF-κB binds to the <t>fascin</t> promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB <t>p50</t> siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P
    Anti Fascin, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti fascin/product/Abcam
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    anti fascin - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    NF-κB binds to the fascin promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB p50 siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P

    Journal: Oncology Letters

    Article Title: Signal transducer and activator of transcription 3 signaling upregulates fascin via nuclear factor-?B in gastric cancer: Implications in cell invasion and migration

    doi: 10.3892/ol.2014.1804

    Figure Lengend Snippet: NF-κB binds to the fascin promoter in response to IL-6 in a STAT3-dependent manner. (A) MKN45 cells were treated with IL-6 for 30 min and ChIP assays were performed with an NF-κB antibody and primers flanking the potential NF-κB binding site. (B) Results from (A) were quantitated by densitometry and expressed as ChIP/input with the untreated sample. (C) MKN45 cells were transfected with STAT3 siRNA or AG490 and cultured for 3 days. Cells were treated with IL-6 for 30 min and ChIP assays were performed with STAT3 or NF-κB antibodies and primers flanking the potential STAT3/NF-κB binding sites. (D) MKN45 cells were transfected with NF-κB p50 siRNA and cultured for 4 days. Whole cell extracts were prepared and western blotting was performed with antibodies against NF-κB p50 and GAPDH. (E) MKN45 cells transfected with control or NF-κB p50 siRNA were treated with IL-6 for 30 min. RNA was subjected to quantitative polymerase chain reaction for fascin and GAPDH. Results were standardized to GAPDH and expressed as fold induction with control siRNA. Values represent the mean ± standard deviation. ** P

    Article Snippet: Antibodies used for western blotting and chromatin immunoprecipitation (ChIP) assays were anti-phosphotyrosine (p)STAT3 (Cell Signaling Technology, Inc., Beverly, MA, USA), anti-STAT3 (Cell Signaling Technology, Inc.), anti-nuclear factor (NF)-κB p50 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-fascin (Abcam, Cambridge, UK), anti-hairy and enhancer of split-1 (Hes-1; Abcam), anti-activated-Notch1 (Abcam), anti-activated-Notch2 (Abcam), anti-matrix metalloproteinase (MMP)-2 and anti-MMP-9 (Cell Signaling Technology, Inc.), anti-GAPDH (Abcam), anti-rabbit immunoglobulin (Ig)G and horseradish peroxidase-linked antibody (Cell Signaling Technology, Inc.).

    Techniques: Chromatin Immunoprecipitation, Binding Assay, Transfection, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Standard Deviation

    The presence of fascin (I), β-tubulin ( II ), HSC70 ( III ), and HSP90 ( IV ) were confirmed by Western blots of PMR-MM7-derived exosomes ( A ) and PMR-MM8-derived exosomes (B). The primary antibodies anti-fascin (1:1000), anti-β-tubulin (1:10.000), and anti-HSP90 (1:2000) were followed by horseradish peroxidase-conjugated goat anti-mouse IgG1. Anti-HSC70 (1:1000) was followed by Envision (DAKO). Blots were incubated with SuperSignal West Pico chemiluminescent substrate and exposed to Hyperfilm ECL (Amersham Biosciences, Buckinghamshire, England).

    Journal: The American Journal of Pathology

    Article Title: Proteomic Analysis of Exosomes Secreted by Human Mesothelioma Cells

    doi:

    Figure Lengend Snippet: The presence of fascin (I), β-tubulin ( II ), HSC70 ( III ), and HSP90 ( IV ) were confirmed by Western blots of PMR-MM7-derived exosomes ( A ) and PMR-MM8-derived exosomes (B). The primary antibodies anti-fascin (1:1000), anti-β-tubulin (1:10.000), and anti-HSP90 (1:2000) were followed by horseradish peroxidase-conjugated goat anti-mouse IgG1. Anti-HSC70 (1:1000) was followed by Envision (DAKO). Blots were incubated with SuperSignal West Pico chemiluminescent substrate and exposed to Hyperfilm ECL (Amersham Biosciences, Buckinghamshire, England).

    Article Snippet: Antibodies used in this study to confirm the proteins detected by MALDI-TOF were: anti-HSC70 (clone 13D3; Affinity BioReagents, Golden, CO), anti-HSP90 (clone AC88; Stressgen, Victoria, Canada), anti-fascin (clone FCN01, Abcam Ltd, Cambridge, UK), and anti-β-tubulin (clone E7, Developmental Studies Hybridoma Bank, Iowa City, IA).

    Techniques: Western Blot, Derivative Assay, Incubation

    Notch4 siRNA regulates the expression of p-Akt, Akt, GSK3, p-GSK3 and fascin in pancreatic cancer cells. The protein expression levels were determined by western blot analysis. Akt, p-Akt and fascin protein expression were markedly decreased in the Notch4 siRNA + docetaxel group compared with that in the Notch4 siRNA and docetaxel groups. GAPDH was used as an internal control to demonstrate equal protein loading. Lane 1, blank control; lane 2, control siRNA; lane 3, Notch4 siRNA; lane 4, docetaxel; lane 5, Notch4 siRNA + docetaxel. GSK3, glycogen synthase kinase 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; p, phosphorylated; siRNA, small interfering RNA.

    Journal: Oncology Letters

    Article Title: Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells

    doi: 10.3892/ol.2016.5097

    Figure Lengend Snippet: Notch4 siRNA regulates the expression of p-Akt, Akt, GSK3, p-GSK3 and fascin in pancreatic cancer cells. The protein expression levels were determined by western blot analysis. Akt, p-Akt and fascin protein expression were markedly decreased in the Notch4 siRNA + docetaxel group compared with that in the Notch4 siRNA and docetaxel groups. GAPDH was used as an internal control to demonstrate equal protein loading. Lane 1, blank control; lane 2, control siRNA; lane 3, Notch4 siRNA; lane 4, docetaxel; lane 5, Notch4 siRNA + docetaxel. GSK3, glycogen synthase kinase 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; p, phosphorylated; siRNA, small interfering RNA.

    Article Snippet: After blocking for 2 h with phosphate-buffered saline containing 0.1% Tween 20 (PBST) and 5% powdered skim milk, the blots were incubated with primary Notch4 (cat. no. ab134831; 1:1,000), p-Akt (cat. no. ab8933; 1:500), Akt (cat. no. ab8805; 1:1,000), GSK3 (cat. no. ab131344; 1:500), p-GSK3 (cat. no. ab28808; 1:500) and fascin (cat. no. ab97753; 1:1,000) antibodies (Abcam, Cambridge, UK) overnight in 5% powdered skim milk buffer, washed thrice with PBST and incubated with horseradish peroxidase-conjugated anti-rabbit (cat. no. 7074) and anti-mouse (cat. no. 7076) IgG secondary antibodies (1:2,000; Cell Signaling Technology, Beverly, MA, USA).

    Techniques: Expressing, Western Blot, Small Interfering RNA

    Western blot analysis of the expression of ZNF139, PDXK, ANXA2 and fascin in (A) SGC7901 cells and (B) transplanted tumors. 1, blank control group; 2, small interfering RNA-ZNF139 group; 3, negative control group. ZNF139, zinc finger protein 139; PDXK,

    Journal: Oncology Letters

    Article Title: Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents

    doi: 10.3892/ol.2015.3421

    Figure Lengend Snippet: Western blot analysis of the expression of ZNF139, PDXK, ANXA2 and fascin in (A) SGC7901 cells and (B) transplanted tumors. 1, blank control group; 2, small interfering RNA-ZNF139 group; 3, negative control group. ZNF139, zinc finger protein 139; PDXK,

    Article Snippet: The gel was sliced and transferred onto the polyvinylidene fluoride membrane, which was initially treated with the closure solution at room temperature and agitated for 1 h. The primary antibodies [rabbit anti-human polyclonal antibodies against ZNF139 (cat. no. ab126124), pyridoxal kinase (PDXK; cat. no. ab38208), annexin A2 (ANXA2; cat. no. ab41803) and fascin (cat. no. ab183891), and mouse anti-human monoclonal antibody against β-actin (cat. no. ab6276); Abcam, Cambridge, UK] were added and then incubated at 4°C overnight.

    Techniques: Western Blot, Expressing, Small Interfering RNA, Negative Control