p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p erk1 2 thr202 tyr204
    P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86/100 stars

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    p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p erk1 2 thr202 tyr204
    LPCAT1 and NRF1 constitute a positive feedback loop through activating <t>the</t> <t>ERK1/2-CREB</t> pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B - C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot ( B ) and qRT-PCR ( C ), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E – F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment ( E ) or CREB knockdown ( F ) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. * p < 0.05, ** p < 0.01, *** p < 0.001
    P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p erk1 2 thr202 tyr204 - by Bioz Stars, 2023-12
    86/100 stars

    Images

    1) Product Images from "Nuclear respiratory factor 1 drives hepatocellular carcinoma progression by activating LPCAT1-ERK1/2-CREB axis"

    Article Title: Nuclear respiratory factor 1 drives hepatocellular carcinoma progression by activating LPCAT1-ERK1/2-CREB axis

    Journal: Biology Direct

    doi: 10.1186/s13062-023-00428-z

    LPCAT1 and NRF1 constitute a positive feedback loop through activating the ERK1/2-CREB pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B - C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot ( B ) and qRT-PCR ( C ), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E – F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment ( E ) or CREB knockdown ( F ) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: LPCAT1 and NRF1 constitute a positive feedback loop through activating the ERK1/2-CREB pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B - C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot ( B ) and qRT-PCR ( C ), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E – F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment ( E ) or CREB knockdown ( F ) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Activity Assay, Over Expression, Western Blot, Quantitative RT-PCR, Activation Assay, Expressing, Binding Assay

    LPCAT1 mediates the oncogenic potential of NRF1 by modulating the ERK1/2-CREB signaling. A - C The proliferation ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by CCK8 ( A - B ) and colony formation assay ( C ). ( D - F ) The migration and invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by transwell ( D - E ) and wound healing assay ( F ). ( G ) The cell cycle and EMT process related genes expression were determined by Western blot in Huh7 and MHCC97H after NRF1 knockdown and LPCAT1 overexpression. H GSEA using KEGG MAPK pathway gene set between high NRF1 group and low NRF1 group in TCGA-LIHC dataset. I The activation of ERK1/2-CREB pathway and NRF1, LPCAT1 protein levels were analysed by Western blot after NRF1 knockdown and LPCAT1 overexpression. J The proliferation ability of Huh7 cells after NRF1 knockdown and PD184352 treatment was determined by CCK8 assay. K The invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and PD184352 treatment was determined by transwell assay. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant
    Figure Legend Snippet: LPCAT1 mediates the oncogenic potential of NRF1 by modulating the ERK1/2-CREB signaling. A - C The proliferation ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by CCK8 ( A - B ) and colony formation assay ( C ). ( D - F ) The migration and invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by transwell ( D - E ) and wound healing assay ( F ). ( G ) The cell cycle and EMT process related genes expression were determined by Western blot in Huh7 and MHCC97H after NRF1 knockdown and LPCAT1 overexpression. H GSEA using KEGG MAPK pathway gene set between high NRF1 group and low NRF1 group in TCGA-LIHC dataset. I The activation of ERK1/2-CREB pathway and NRF1, LPCAT1 protein levels were analysed by Western blot after NRF1 knockdown and LPCAT1 overexpression. J The proliferation ability of Huh7 cells after NRF1 knockdown and PD184352 treatment was determined by CCK8 assay. K The invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and PD184352 treatment was determined by transwell assay. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant

    Techniques Used: Over Expression, Colony Assay, Migration, Wound Healing Assay, Expressing, Western Blot, Activation Assay, CCK-8 Assay, Transwell Assay

    NRF1 promotes HCC growth in vivo. Lentivirus was used to establish stable NRF1 knockdown Huh7 cell lines and tumor growth ability was assessed with subcutaneous xenograft model. A The image of subcutaneous xenograft tumors were shown. B The tumor volume was measured every three days. C The mass of the subcutaneous xenograft tumors was weighed after excision. D Representative images of IHC staining and the IHC scores of NRF1, LPCAT1, p-ERK1/2, p-CREB, Ki67 and Vimentin of the tumors. Scale bar: 50 μm. E NRF1, LPCAT1, p-ERK1/2, ERK1/2, p-CREB, CREB, PCNA and Vimentin were analyzed with Western blot. Bar graphs showed the quantitative results. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: NRF1 promotes HCC growth in vivo. Lentivirus was used to establish stable NRF1 knockdown Huh7 cell lines and tumor growth ability was assessed with subcutaneous xenograft model. A The image of subcutaneous xenograft tumors were shown. B The tumor volume was measured every three days. C The mass of the subcutaneous xenograft tumors was weighed after excision. D Representative images of IHC staining and the IHC scores of NRF1, LPCAT1, p-ERK1/2, p-CREB, Ki67 and Vimentin of the tumors. Scale bar: 50 μm. E NRF1, LPCAT1, p-ERK1/2, ERK1/2, p-CREB, CREB, PCNA and Vimentin were analyzed with Western blot. Bar graphs showed the quantitative results. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: In Vivo, Immunohistochemistry, Western Blot

    p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p erk1 2 thr202 tyr204
    LPCAT1 and NRF1 constitute a positive feedback loop through activating <t>the</t> <t>ERK1/2-CREB</t> pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B - C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot ( B ) and qRT-PCR ( C ), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E – F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment ( E ) or CREB knockdown ( F ) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. * p < 0.05, ** p < 0.01, *** p < 0.001
    P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p erk1 2 thr202 tyr204 - by Bioz Stars, 2023-12
    86/100 stars

    Images

    1) Product Images from "Nuclear respiratory factor 1 drives hepatocellular carcinoma progression by activating LPCAT1-ERK1/2-CREB axis"

    Article Title: Nuclear respiratory factor 1 drives hepatocellular carcinoma progression by activating LPCAT1-ERK1/2-CREB axis

    Journal: Biology Direct

    doi: 10.1186/s13062-023-00428-z

    LPCAT1 and NRF1 constitute a positive feedback loop through activating the ERK1/2-CREB pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B - C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot ( B ) and qRT-PCR ( C ), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E – F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment ( E ) or CREB knockdown ( F ) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: LPCAT1 and NRF1 constitute a positive feedback loop through activating the ERK1/2-CREB pathway. A NRF1 activity difference was analysed between high LPCAT1 and low LPCAT1 group with GSEA in TCGA-LIHC dataset. B - C NRF1 protein and mRNA levels in Huh7 and MHCC97H after LPCAT1 overexpression or knockdown were determined by Western blot ( B ) and qRT-PCR ( C ), respectively. D GSEA was performed to analyse MAPK activation in high and low LPCAT1 expression group. E – F The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 overexpression and PD184352 treatment ( E ) or CREB knockdown ( F ) were analysed by Western blot. G The activation of ERK1/2-CREB pathway and NRF1 protein levels after LPCAT1 knockdown and DPPC supplementation were analysed by Western blot. H ChIP-qPCR was applied to analyse the binding of CREB on NRF1 promoter after LPCAT1 knockdown or overexpression. α-CREB, anti-CREB antibody. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Activity Assay, Over Expression, Western Blot, Quantitative RT-PCR, Activation Assay, Expressing, Binding Assay

    LPCAT1 mediates the oncogenic potential of NRF1 by modulating the ERK1/2-CREB signaling. A - C The proliferation ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by CCK8 ( A - B ) and colony formation assay ( C ). ( D - F ) The migration and invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by transwell ( D - E ) and wound healing assay ( F ). ( G ) The cell cycle and EMT process related genes expression were determined by Western blot in Huh7 and MHCC97H after NRF1 knockdown and LPCAT1 overexpression. H GSEA using KEGG MAPK pathway gene set between high NRF1 group and low NRF1 group in TCGA-LIHC dataset. I The activation of ERK1/2-CREB pathway and NRF1, LPCAT1 protein levels were analysed by Western blot after NRF1 knockdown and LPCAT1 overexpression. J The proliferation ability of Huh7 cells after NRF1 knockdown and PD184352 treatment was determined by CCK8 assay. K The invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and PD184352 treatment was determined by transwell assay. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant
    Figure Legend Snippet: LPCAT1 mediates the oncogenic potential of NRF1 by modulating the ERK1/2-CREB signaling. A - C The proliferation ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by CCK8 ( A - B ) and colony formation assay ( C ). ( D - F ) The migration and invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and LPCAT1 overexpression were analysed by transwell ( D - E ) and wound healing assay ( F ). ( G ) The cell cycle and EMT process related genes expression were determined by Western blot in Huh7 and MHCC97H after NRF1 knockdown and LPCAT1 overexpression. H GSEA using KEGG MAPK pathway gene set between high NRF1 group and low NRF1 group in TCGA-LIHC dataset. I The activation of ERK1/2-CREB pathway and NRF1, LPCAT1 protein levels were analysed by Western blot after NRF1 knockdown and LPCAT1 overexpression. J The proliferation ability of Huh7 cells after NRF1 knockdown and PD184352 treatment was determined by CCK8 assay. K The invasion ability of Huh7 and MHCC97H cells after NRF1 knockdown and PD184352 treatment was determined by transwell assay. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant

    Techniques Used: Over Expression, Colony Assay, Migration, Wound Healing Assay, Expressing, Western Blot, Activation Assay, CCK-8 Assay, Transwell Assay

    NRF1 promotes HCC growth in vivo. Lentivirus was used to establish stable NRF1 knockdown Huh7 cell lines and tumor growth ability was assessed with subcutaneous xenograft model. A The image of subcutaneous xenograft tumors were shown. B The tumor volume was measured every three days. C The mass of the subcutaneous xenograft tumors was weighed after excision. D Representative images of IHC staining and the IHC scores of NRF1, LPCAT1, p-ERK1/2, p-CREB, Ki67 and Vimentin of the tumors. Scale bar: 50 μm. E NRF1, LPCAT1, p-ERK1/2, ERK1/2, p-CREB, CREB, PCNA and Vimentin were analyzed with Western blot. Bar graphs showed the quantitative results. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: NRF1 promotes HCC growth in vivo. Lentivirus was used to establish stable NRF1 knockdown Huh7 cell lines and tumor growth ability was assessed with subcutaneous xenograft model. A The image of subcutaneous xenograft tumors were shown. B The tumor volume was measured every three days. C The mass of the subcutaneous xenograft tumors was weighed after excision. D Representative images of IHC staining and the IHC scores of NRF1, LPCAT1, p-ERK1/2, p-CREB, Ki67 and Vimentin of the tumors. Scale bar: 50 μm. E NRF1, LPCAT1, p-ERK1/2, ERK1/2, p-CREB, CREB, PCNA and Vimentin were analyzed with Western blot. Bar graphs showed the quantitative results. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: In Vivo, Immunohistochemistry, Western Blot

    anti erk1 2 p thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti erk1 2 p thr202 tyr204
    Anti Erk1 2 P Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti erk1 2 p thr202 tyr204/product/Cell Signaling Technology Inc
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    rabbit monoclonal anti p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti p erk1 2 thr202 tyr204
    Rabbit Monoclonal Anti P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
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    rabbit monoclonal anti p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti p erk1 2 thr202 tyr204
    Rabbit Monoclonal Anti P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p erk1 2 thr202 tyr204
    P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    p erk1 2 thr202 tyr204 - by Bioz Stars, 2023-12
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    anti p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti p erk1 2 thr202 tyr204
    Anti P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti p erk1 2 thr202 tyr204 - by Bioz Stars, 2023-12
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    p mapk erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p mapk erk1 2 thr202 tyr204
    P Mapk Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p erk1 2 thr202 tyr204  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p erk1 2 thr202 tyr204
    A Luciferase-labeled MDA-MB-231 (MDA-MB-231-Luciferase) cells were injected into 10 female nude mice via tail vein injection. Lung metastasis was monitored by bioluminescence using an in vivo imaging system in the left panel. Number of metastatic nodules in the lung. Error bars represent the mean ± SD. ( * means P < 0. 001). B H&E staining of lungs from the indicated groups upon harvest (day 21). Scale bars, 100 µm. C , D The expression of p-FAK <t>and</t> <t>p-ERK1/2</t> in nude mice lung tissue was detected by immunohistochemistry. Scale bars, 100 µm. E The schematic diagram of the role of BCKDK/talin1 signaling axis in affecting the adhesion mechanism of breast cancer cells.
    P Erk1 2 Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p erk1 2 thr202 tyr204/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p erk1 2 thr202 tyr204 - by Bioz Stars, 2023-12
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    Images

    1) Product Images from "BCKDK regulates breast cancer cell adhesion and tumor metastasis by inhibiting TRIM21 ubiquitinate talin1"

    Article Title: BCKDK regulates breast cancer cell adhesion and tumor metastasis by inhibiting TRIM21 ubiquitinate talin1

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-023-05944-4

    A Luciferase-labeled MDA-MB-231 (MDA-MB-231-Luciferase) cells were injected into 10 female nude mice via tail vein injection. Lung metastasis was monitored by bioluminescence using an in vivo imaging system in the left panel. Number of metastatic nodules in the lung. Error bars represent the mean ± SD. ( * means P < 0. 001). B H&E staining of lungs from the indicated groups upon harvest (day 21). Scale bars, 100 µm. C , D The expression of p-FAK and p-ERK1/2 in nude mice lung tissue was detected by immunohistochemistry. Scale bars, 100 µm. E The schematic diagram of the role of BCKDK/talin1 signaling axis in affecting the adhesion mechanism of breast cancer cells.
    Figure Legend Snippet: A Luciferase-labeled MDA-MB-231 (MDA-MB-231-Luciferase) cells were injected into 10 female nude mice via tail vein injection. Lung metastasis was monitored by bioluminescence using an in vivo imaging system in the left panel. Number of metastatic nodules in the lung. Error bars represent the mean ± SD. ( * means P < 0. 001). B H&E staining of lungs from the indicated groups upon harvest (day 21). Scale bars, 100 µm. C , D The expression of p-FAK and p-ERK1/2 in nude mice lung tissue was detected by immunohistochemistry. Scale bars, 100 µm. E The schematic diagram of the role of BCKDK/talin1 signaling axis in affecting the adhesion mechanism of breast cancer cells.

    Techniques Used: Luciferase, Labeling, Injection, In Vivo Imaging, Staining, Expressing, Immunohistochemistry

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