Journal: PLoS ONE

Article Title: Pressure Load: The Main Factor for Altered Gene Expression in Right Ventricular Hypertrophy in Chronic Hypoxic Rats

doi: 10.1371/journal.pone.0015859

Figure Lengend Snippet: Changes in gene expression by gene chip analysis – hypoxia compared to normoxia.

Article Snippet: 10359-1-AP, Ptg.Lab, IL, USA), anti-tissue transglutaminase (tTG) (TGase II Ab-2 (TG-100), Thermo Scientific, CA, USA), anti-endothelin receptor B (ET B ) (cat.nr. ab1921, Abcam, UK).

Techniques: Expressing, Binding Assay

Journal: PLoS ONE

Article Title: Pressure Load: The Main Factor for Altered Gene Expression in Right Ventricular Hypertrophy in Chronic Hypoxic Rats

doi: 10.1371/journal.pone.0015859

Figure Lengend Snippet: Proteins participating in metabolism A: ACAA2, B: HADHA and C: aquaporin 7 show tendency to downregulation by hypoxia. D: monoamine oxidase A, E: tissue transglutaminase and F: endothelin receptor B were all upregulated by hypoxia. ACAA2: acetyl-Coenzyme A acyltransferase 2, HADHA: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit. Values are means ± SE and are calculated as percent of normoxia 1 week. n = 6 in both groups at all time points. *P<0.05 vs. normoxia at same time point.

Article Snippet: 10359-1-AP, Ptg.Lab, IL, USA), anti-tissue transglutaminase (tTG) (TGase II Ab-2 (TG-100), Thermo Scientific, CA, USA), anti-endothelin receptor B (ET B ) (cat.nr. ab1921, Abcam, UK).

Techniques:

Journal: Cell Stem Cell

Article Title: Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development

doi: 10.1016/j.stem.2020.08.004

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Endothelin B Receptor , Abcam , Cat# ab117529, RRID: AB_10902070.

Techniques: Recombinant, Isolation, Multiplex Assay, Software

Journal: Frontiers in Physiology

Article Title: Action of Actomyosin Contraction With Shh Modulation Drive Epithelial Folding in the Circumvallate Papilla

doi: 10.3389/fphys.2020.00936

Figure Lengend Snippet: (A) The circumvallate papilla (CVP) epithelium at E12.5 is a stratified placode, while non-CVP epithelium is monolayered. (B) At E13.0, epithelium started to fold outward at the medial region of the placode. A low cell density region is observed in the underlying mesenchyme at E13.0. (C) Dome-shape and lateral trenches are observed with monolayered or pseudostratified CVP epithelium at E13.5. (D,E) Endothelin receptor B (ETB) is located in the lateral tongue mesenchyme at E12.5 and underlying mesenchyme of CVP at E13.0. (F) ETB is detected in the stromal core of CVP at E13.5. (G,H) Protein gene product 9.5 (PGP9.5) is located in the lateral tongue mesenchyme at E12.5 and the underlying mesenchyme of CVP at E13.0. (I) The stromal core of CVP is marked by PGP9.5 at E13.5. (J) Fibronectin is located throughout the tongue mesenchyme at E12.5. (K) Fibronectin density is relatively low in the presence of neuroblasts (marked by ETB) at E13.0 (according to S1C) (arrowhead). (L) Fibronectin localization in the stromal core is relatively weaker than the surrounding mesenchyme at E13.5. (M) E-cadherin is located throughout the entire epithelium at E12.5. (N) E-cadherin is relatively enriched in basal and suprabasal cells of placodes at E13.0. (O) At E13.5, slight enrichment of E-cadherin is found in suprabasal cells. (P) F-actin accumulation is detected at the basal site of basal cells at E12.5. (Q) At E13.0, F-actin strongly accumulates at the basal site of basal cells in the medial region of CVP placode. (R) F-actin is enriched at the basal (site in the dome-shape region and at the apical site of the trench region epithelial cells at E13.5. (S,T) Phosphomyosin light chain II (pMLC) is enriched at the basal site of basal cells at E12.5 and strictly accumulated at the basal site of the medial region at E13.0. (U) pMLC is weakly detected in CVP epithelium at E13.5. (V–X) pMLC is co-accumulated with F-actin at E12.5 and E13.0 but not E13.5. Scale bar = 100 μm in panels (A–O) , 50 μm in panels (P–X) . Arrows indicate the cells which go through apical and basal constriction. Arrowhead indicates the low-density region of fibronectin localization. Thick dotted lines mark the border between developing tongue epithelium and mesenchyme. Thin dotted lines indicate individual cell shapes which go through apical and basal constriction.)

Article Snippet: After citrate buffer (pH 6.0), Specimens were blocked using 1% goat serum or 5% bovine serum albumin in PBS, incubated with antibodies against endothelin receptor B (1:50, Abcam, United Kingdom), PGP9.5 (1:100, Abcam, United Kingdom), fibronectin (1:100, BD Bioscience, United States), E-cadherin (1:100, R&D Systems, United States), phosphomyosin light chain II (1:50, Cell Signaling Technology, United States), β-catenin (1:100, Santa Cruz, United States), and ZO-1 (1:100, Invitrogen, United States).

Techniques: