Journal: Journal of Molecular Histology

Article Title: EGF-receptor phosphorylation and downstream signaling are activated by genistein during subacute liver damage

doi: 10.1007/s10735-023-10127-8

Figure Lengend Snippet: Effect of genistein on expression and phosphorylation of EGFR during experimental CCl 4 -induced subacute liver damage. An increase in total protein and phosphorylation from livers with CCl 4 -induced subacute liver damage was observed. Genistein significantly increased total protein and pY845 and pY1068 EGFR. The analysis was determined by Western blot a semiquantitative analysis of the expression levels of p‑EGFR (Y845, Y992 and Y1068), β-actin was used as a loading control. Bars show the mean values ± standard deviations of the band density normalized to the total protein. n = 8, *p < 0.05 as compared to control group; #p < 0.05 as compared to liver damage group, respectively using an ANOVA and Tukey’s test

Article Snippet: The antibodies used in the experiments were as follows: EGFR (1:500, #4267, Cell Signaling Technology, Danvers, MA, USA), phospho-EGFR Y845 (1:500, #2231, Cell Signaling), phospho-EGFR Y992 (1:500, #2235, Cell Signaling), phospho-EGFR Y1068 (1:500, #2236, Cell Signaling), AKT (1:500, #9272, Cell Signaling), phospho-AKT (1:500, #9271, Cell Signaling), STAT5 (1:500, #9363, Cell Signaling), phosphor-STAT5 (1:500, #9351, Cell Signaling), PLC-γ1 (1:500, sc-7290, Santa Cruz Biotechnology, CA, USA), phospho-PLC-γ1 (1:500, sc-136,186, Santa Cruz Biotechnology), β-actin (1:500, sc-47,778 S, anta Cruz Biotechnology), secondary antibody anti-rabbit HRP (1:2000, #7074, Cell signaling, Technology Inc.) and secondary antibody anti-mouse HRP (1:1500, A2304, Sigma-Aldrich).

Techniques: Expressing, Western Blot

Journal: Journal of Molecular Histology

Article Title: EGF-receptor phosphorylation and downstream signaling are activated by genistein during subacute liver damage

doi: 10.1007/s10735-023-10127-8

Figure Lengend Snippet: Effect of genistein on expression and phosphorylation of EGFR during experimental CCl 4 -induced subacute liver damage. An increase in total protein and phosphorylation from livers with CCl 4 -induced subacute liver damage was observed. Genistein significantly increased total protein and pY845 and pY1068 EGFR. The analysis was determined by Western blot a semiquantitative analysis of the expression levels of p‑EGFR (Y845, Y992 and Y1068), β-actin was used as a loading control. Bars show the mean values ± standard deviations of the band density normalized to the total protein. n = 8, *p < 0.05 as compared to control group; #p < 0.05 as compared to liver damage group, respectively using an ANOVA and Tukey’s test

Article Snippet: The antibodies used in the experiments were as follows: EGFR (1:500, #4267, Cell Signaling Technology, Danvers, MA, USA), phospho-EGFR Y845 (1:500, #2231, Cell Signaling), phospho-EGFR Y992 (1:500, #2235, Cell Signaling), phospho-EGFR Y1068 (1:500, #2236, Cell Signaling), AKT (1:500, #9272, Cell Signaling), phospho-AKT (1:500, #9271, Cell Signaling), STAT5 (1:500, #9363, Cell Signaling), phosphor-STAT5 (1:500, #9351, Cell Signaling), PLC-γ1 (1:500, sc-7290, Santa Cruz Biotechnology, CA, USA), phospho-PLC-γ1 (1:500, sc-136,186, Santa Cruz Biotechnology), β-actin (1:500, sc-47,778 S, anta Cruz Biotechnology), secondary antibody anti-rabbit HRP (1:2000, #7074, Cell signaling, Technology Inc.) and secondary antibody anti-mouse HRP (1:1500, A2304, Sigma-Aldrich).

Techniques: Expressing, Western Blot

Journal: Journal of Molecular Histology

Article Title: EGF-receptor phosphorylation and downstream signaling are activated by genistein during subacute liver damage

doi: 10.1007/s10735-023-10127-8

Figure Lengend Snippet: Effect of genistein on expression and phosphorylation of EGFR during experimental CCl 4 -induced subacute liver damage. An increase in total protein and phosphorylation from livers with CCl 4 -induced subacute liver damage was observed. Genistein significantly increased total protein and pY845 and pY1068 EGFR. The analysis was determined by Western blot a semiquantitative analysis of the expression levels of p‑EGFR (Y845, Y992 and Y1068), β-actin was used as a loading control. Bars show the mean values ± standard deviations of the band density normalized to the total protein. n = 8, *p < 0.05 as compared to control group; #p < 0.05 as compared to liver damage group, respectively using an ANOVA and Tukey’s test

Article Snippet: The antibodies used in the experiments were as follows: EGFR (1:500, #4267, Cell Signaling Technology, Danvers, MA, USA), phospho-EGFR Y845 (1:500, #2231, Cell Signaling), phospho-EGFR Y992 (1:500, #2235, Cell Signaling), phospho-EGFR Y1068 (1:500, #2236, Cell Signaling), AKT (1:500, #9272, Cell Signaling), phospho-AKT (1:500, #9271, Cell Signaling), STAT5 (1:500, #9363, Cell Signaling), phosphor-STAT5 (1:500, #9351, Cell Signaling), PLC-γ1 (1:500, sc-7290, Santa Cruz Biotechnology, CA, USA), phospho-PLC-γ1 (1:500, sc-136,186, Santa Cruz Biotechnology), β-actin (1:500, sc-47,778 S, anta Cruz Biotechnology), secondary antibody anti-rabbit HRP (1:2000, #7074, Cell signaling, Technology Inc.) and secondary antibody anti-mouse HRP (1:1500, A2304, Sigma-Aldrich).

Techniques: Expressing, Western Blot

Journal: eLife

Article Title: pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells

doi: 10.7554/eLife.82863

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-EGFR antibody (rabbit monoclonal) , Cell Signaling Technology , Cat # 4267 , Used at 1:1000 for western blotting.

Techniques: Recombinant, Plasmid Preparation, Construct, CRISPR, Modification, Sequencing, Western Blot, Immunostaining, Clone Assay, Software