Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dual-process brain mitochondria isolation preserves function and clarifies protein composition

doi: 10.1073/pnas.2019046118

Figure Lengend Snippet: Citation information for cellular localization of proteins previously reported as localizing in mitochondria but not observed in ultrapurified mitochondria here

Article Snippet: Antibodies included Anti-Ca v 1.2 (Alomone Labs, AGP-001), Gapdh (Santa Cruz, sc-365062), Sdha (Cell Signaling, 11998), Tom20 (Cell Signaling, 42406), Facl4 (ThermoFisher, PA5-27137), Tom70 (Santa Cruz Biotechnology, sc-390545, a gift from Stefan Strack, University of Iowa, Iowa City, IA), Pdh E1α (Cell Signaling, 3205), Vdac (Cell Signaling, 4661), Serca2 (Cell Signaling, 4388), Erlin2 (Cell Signaling, 2959), Psd95 (Cell Signaling, 2507), β-actin (Santa Cruz, sc-47778), Eaat1 (Alomone, AGC-021), Glut3 (Millipore, AB1344), Mct1 (Alomone, AMT-011), Asic1 (biorbyt, orb13257), K v 1.3 (Alomone, APC-002), Ryr1 (Cell Signaling, 8153), mitoK (Sigma, HPA010980), Slc25a12 (Alomone, ANT-112), Slc25a22 (Proteintech, 25402-1-AP), Mcu (Sigma, HPA016480, a gift from Yuriy Usachev, University of Iowa), Mpc1 (Cell Signaling, 14462S), and Mpc2 (Cell Signaling, 46141S).

Techniques: Fractionation

Journal: Journal of neuroscience research

Article Title: Glial fibrillary acidic protein promoter determines transgene expression in satellite glial cells following intraganglionic adeno-associated virus delivery in adult rats

doi: 10.1002/jnr.24183

Figure Lengend Snippet: Primary antibodies and IgG controls used in this study

Article Snippet: EAAT1 , Rabbit polyclonal , AlomoneLabs/AGC-021 , 1:100 (IHC).

Techniques:

Journal: Journal of neuroscience research

Article Title: Glial fibrillary acidic protein promoter determines transgene expression in satellite glial cells following intraganglionic adeno-associated virus delivery in adult rats

doi: 10.1002/jnr.24183

Figure Lengend Snippet: Primary antibodies and IgG controls used in this study

Article Snippet: For double-label colocalization, images from the same section but showing different antigen signals were overlaid. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody a Host Supplier/Catalog# b Dilution GFP Mouse monoclonal SCB/sc-32422 1:200 (IHC); 1:400 (Wb) GFP Rabbit polyclonal CS/2555 1:200 (IHC) mCherry Rabbit polyclonal LF/PA534974 1:200 (IHC); 1:400 (Wb) Hmgcs1 Mouse monoclonal SCB/sc-393256 1:200 (IHC) Tubb3 Mouse monoclonal SCB/sc-80016 1:400 (IHC) Vimentin Goat polyclonal SCB/sc-12886R 1:600 (IHC) GFAP Rabbit polyclonal Dako/Z0334 1:1000 (IHC); 1:2000 (Wb) GS Rabbit polyclonal SCB/sc-6640R 1:600 (IHC) Iba1 Rabbit polyclonal Wako/019-19741 1:1000 (IHC) K ir 4.1 Guinea pig polyclonal AlomoneLabs/AGP-012 1:100 (IHC) EAAT1 Rabbit polyclonal AlomoneLabs/AGC-021 1:100 (IHC) S100 Mouse monoclonal NeoMarkers/MS296Pl 1:200 (IHC) MBP Goat polyclonal SCB/sc-13912 1:1000 (IHC) GAPDH Mouse monoclonal PT/2555 1:2000 (Wb) CD6 Mouse monoclonal Millipore/CBL554 1:100 (IHC) CD8 Mouse monoclonal Millrpore/217580 1:100 (IHC) IgG control Mouse LF/31903 1:100~400 IgG control Goat LF/31245 1:500~1000 IgG control Rabbit LF/MA5-16384 1:200~1000 Open in a separate window a Antibody abbreviations: Hmgcs1, HMG CoA synthase 1 ; Tubb3, β3 - Tubulin ; GFAP, Glial fibrillary acidic protein ; GS, Glutamine synthetase ; Iba1, Ionized calcium binding adaptor molecule 1 ; K ir 4.1, ATP - sensitive inward rectifier potassium channel 10 ; EEAT1, Excitatory amino acid transporter 1 ; S100, S - 100 calcium-binding protein ; MBP, Myelin basic protein ; GAPDH , Glyceraldehyde 3 - phosphate dehydrogenase ; CD6, Cluster of differentiation 6 ; and CD8, Cluster of differentiation 8 . b SCB, Santa Cruz Biotechnology, Santa Cruz, CA; CS, Cell signaling, Danvers, MA; LF, life Technologies, Carlsbad, CA; Dako, Carpintena, CA; Wako, Richmond, VA; Alomone Labs, Jerusalem, Israel; NeoMarkers, Fremont, CA; PT, Proteintech, Rosemont, IL; Millipore, Billenca, MA.

Techniques:

Journal: Journal of neuroscience research

Article Title: Glial fibrillary acidic protein promoter determines transgene expression in satellite glial cells following intraganglionic adeno-associated virus delivery in adult rats

doi: 10.1002/jnr.24183

Figure Lengend Snippet: (a) Representative results of double immunolabeling of EGFP with multiple authentic glial cell markers (Vimentin, Iba1, GS, S100, EAAT1, Kir4.1, and Hmgcs1) labeled on the top and filtering (marker, EGFP, or merge) indicated at the left of the panels, (b) EGFP expression was observed in the section of sciatic nerve, but EGFP was not costained with MBP, a marker of myelin. Right panels are magnified inset of b). Scale bar: 100µm for all panels.

Article Snippet: For double-label colocalization, images from the same section but showing different antigen signals were overlaid. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody a Host Supplier/Catalog# b Dilution GFP Mouse monoclonal SCB/sc-32422 1:200 (IHC); 1:400 (Wb) GFP Rabbit polyclonal CS/2555 1:200 (IHC) mCherry Rabbit polyclonal LF/PA534974 1:200 (IHC); 1:400 (Wb) Hmgcs1 Mouse monoclonal SCB/sc-393256 1:200 (IHC) Tubb3 Mouse monoclonal SCB/sc-80016 1:400 (IHC) Vimentin Goat polyclonal SCB/sc-12886R 1:600 (IHC) GFAP Rabbit polyclonal Dako/Z0334 1:1000 (IHC); 1:2000 (Wb) GS Rabbit polyclonal SCB/sc-6640R 1:600 (IHC) Iba1 Rabbit polyclonal Wako/019-19741 1:1000 (IHC) K ir 4.1 Guinea pig polyclonal AlomoneLabs/AGP-012 1:100 (IHC) EAAT1 Rabbit polyclonal AlomoneLabs/AGC-021 1:100 (IHC) S100 Mouse monoclonal NeoMarkers/MS296Pl 1:200 (IHC) MBP Goat polyclonal SCB/sc-13912 1:1000 (IHC) GAPDH Mouse monoclonal PT/2555 1:2000 (Wb) CD6 Mouse monoclonal Millipore/CBL554 1:100 (IHC) CD8 Mouse monoclonal Millrpore/217580 1:100 (IHC) IgG control Mouse LF/31903 1:100~400 IgG control Goat LF/31245 1:500~1000 IgG control Rabbit LF/MA5-16384 1:200~1000 Open in a separate window a Antibody abbreviations: Hmgcs1, HMG CoA synthase 1 ; Tubb3, β3 - Tubulin ; GFAP, Glial fibrillary acidic protein ; GS, Glutamine synthetase ; Iba1, Ionized calcium binding adaptor molecule 1 ; K ir 4.1, ATP - sensitive inward rectifier potassium channel 10 ; EEAT1, Excitatory amino acid transporter 1 ; S100, S - 100 calcium-binding protein ; MBP, Myelin basic protein ; GAPDH , Glyceraldehyde 3 - phosphate dehydrogenase ; CD6, Cluster of differentiation 6 ; and CD8, Cluster of differentiation 8 . b SCB, Santa Cruz Biotechnology, Santa Cruz, CA; CS, Cell signaling, Danvers, MA; LF, life Technologies, Carlsbad, CA; Dako, Carpintena, CA; Wako, Richmond, VA; Alomone Labs, Jerusalem, Israel; NeoMarkers, Fremont, CA; PT, Proteintech, Rosemont, IL; Millipore, Billenca, MA.

Techniques: Immunolabeling, Labeling, Marker, Expressing