Structured Review

Santa Cruz Biotechnology anti dynein
NPs synthesis and characterization (A) NP-complex synthesis process entails several consecutive steps, where at each step, a single component is added. (B) Mean anchoring distance between neighboring PEG-NLSs, ξ*, against the concentration of NLS. The grey dots correspond to extrapolated values which were calculated from the fit of 〈N〉 vs. [NLS] (see Table 1 , SI.1 and Fig. S1.2 ). Error bars indicate the standard deviations for 3 experiments. (C) Western blot (WB) analysis results demonstrating the recruitment of <t>importin-α2</t> (a) and mammalian <t>Dynein</t> motors (b) to the NPs after incubation in Hela cells extract. Group 1 (in both (a) and (b)) refers to Hela cells extract without NPs, group 2 refers to NPs coated with Biotin-PEG-SH, and group 3 refers to PEG-NLS coated NPs. The concentration of cells extract used in WB is 3.4 mg/ml.
Anti Dynein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti dynein/product/Santa Cruz Biotechnology
Average 95 stars, based on 3 article reviews
Price from $9.99 to $1999.99
anti dynein - by Bioz Stars, 2022-11
95/100 stars

Images

1) Product Images from "Nano-particles carried by multiple dynein motors: A Self-Regulating Nano-Machine"

Article Title: Nano-particles carried by multiple dynein motors: A Self-Regulating Nano-Machine

Journal: bioRxiv

doi: 10.1101/2020.07.09.194720

NPs synthesis and characterization (A) NP-complex synthesis process entails several consecutive steps, where at each step, a single component is added. (B) Mean anchoring distance between neighboring PEG-NLSs, ξ*, against the concentration of NLS. The grey dots correspond to extrapolated values which were calculated from the fit of 〈N〉 vs. [NLS] (see Table 1 , SI.1 and Fig. S1.2 ). Error bars indicate the standard deviations for 3 experiments. (C) Western blot (WB) analysis results demonstrating the recruitment of importin-α2 (a) and mammalian Dynein motors (b) to the NPs after incubation in Hela cells extract. Group 1 (in both (a) and (b)) refers to Hela cells extract without NPs, group 2 refers to NPs coated with Biotin-PEG-SH, and group 3 refers to PEG-NLS coated NPs. The concentration of cells extract used in WB is 3.4 mg/ml.
Figure Legend Snippet: NPs synthesis and characterization (A) NP-complex synthesis process entails several consecutive steps, where at each step, a single component is added. (B) Mean anchoring distance between neighboring PEG-NLSs, ξ*, against the concentration of NLS. The grey dots correspond to extrapolated values which were calculated from the fit of 〈N〉 vs. [NLS] (see Table 1 , SI.1 and Fig. S1.2 ). Error bars indicate the standard deviations for 3 experiments. (C) Western blot (WB) analysis results demonstrating the recruitment of importin-α2 (a) and mammalian Dynein motors (b) to the NPs after incubation in Hela cells extract. Group 1 (in both (a) and (b)) refers to Hela cells extract without NPs, group 2 refers to NPs coated with Biotin-PEG-SH, and group 3 refers to PEG-NLS coated NPs. The concentration of cells extract used in WB is 3.4 mg/ml.

Techniques Used: Concentration Assay, Western Blot, Incubation

2) Product Images from "Study on the Dynamic Changes in Synaptic Vesicle-Associated Protein and Axonal Transport Protein Combined with LPS Neuroinflammation Model"

Article Title: Study on the Dynamic Changes in Synaptic Vesicle-Associated Protein and Axonal Transport Protein Combined with LPS Neuroinflammation Model

Journal: ISRN Neurology

doi: 10.1155/2013/496079

Striatal changes in proteins involved in axonal transport at different weeks after LPS injection. Levels of dynein and dynactin were reduced at 3 w. Levels of dynein were reduced at 6 w. At the same time, levels of β -tubulin were reduced in 2 early weeks. Optical densities of LPS injection conditions were normalized by the averaged value of saline injection. Data are shown as mean ± SD n = 3~6, * P
Figure Legend Snippet: Striatal changes in proteins involved in axonal transport at different weeks after LPS injection. Levels of dynein and dynactin were reduced at 3 w. Levels of dynein were reduced at 6 w. At the same time, levels of β -tubulin were reduced in 2 early weeks. Optical densities of LPS injection conditions were normalized by the averaged value of saline injection. Data are shown as mean ± SD n = 3~6, * P

Techniques Used: Injection

Nigral changes in proteins involved in axonal transport at different weeks after LPS injection. Levels of dynein were increased at 6 w in the SN. Levels of dynactin increased at 3 w, whereas levels of dynein and β -tubulin were increased at 3 w. Optical densities of LPS injection conditions were normalized by the averaged value of saline injection. Data are shown as mean ± SD n = 3~6, * P
Figure Legend Snippet: Nigral changes in proteins involved in axonal transport at different weeks after LPS injection. Levels of dynein were increased at 6 w in the SN. Levels of dynactin increased at 3 w, whereas levels of dynein and β -tubulin were increased at 3 w. Optical densities of LPS injection conditions were normalized by the averaged value of saline injection. Data are shown as mean ± SD n = 3~6, * P

Techniques Used: Injection

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    Santa Cruz Biotechnology dynein ic1 2 cytosolic antibody
    Dynein Ic1 2 Cytosolic Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynein ic1 2 cytosolic antibody/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
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    92
    Santa Cruz Biotechnology dynein hc antibody
    OXI inhibits autophagosome-lysosome fusion by repressing PRDX2/RAB7A axis in CRC cells. (A) RKO and HCT116 cells were treated with OXI (30 μM) or RAPA (100 nM) for 24 h, and then dyed with LysoTracker (75 nM) for 30 min. (B) Western blotting analysis of <t>Myosin,</t> <t>SNAP29,</t> Dynactin, <t>Dynein</t> <t>HC</t> and RAB7A treated with or without OXI (30 μM). (C) Western blotting analysis of RAB7A in HCT116 and RKO cells transfected with Vector or HA-PRDX2 and treated with OXI (30 μM). (D) Western blotting analysis of RAB7A protein level in HCT116 and RKO cells transfected with HA-RAB7A plasmids for 48 h. (E-F) HCT116 and RKO cells were transiently transfected with mRFP-GFP-LC3 and HA-RAB7A, then treated with OXI (30 μM). Scale bars, 10 μm. (G) Quantification of the ratio of yellow puncta (autophagosome) versus red puncta (autolysosome). (H) The expression level of RAB7A in xenografts was evaluated by IHC. Scale bar: 50 μm. All data are means ± SD.
    Dynein Hc Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynein hc antibody/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dynein hc antibody - by Bioz Stars, 2022-11
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    Image Search Results


    OXI inhibits autophagosome-lysosome fusion by repressing PRDX2/RAB7A axis in CRC cells. (A) RKO and HCT116 cells were treated with OXI (30 μM) or RAPA (100 nM) for 24 h, and then dyed with LysoTracker (75 nM) for 30 min. (B) Western blotting analysis of Myosin, SNAP29, Dynactin, Dynein HC and RAB7A treated with or without OXI (30 μM). (C) Western blotting analysis of RAB7A in HCT116 and RKO cells transfected with Vector or HA-PRDX2 and treated with OXI (30 μM). (D) Western blotting analysis of RAB7A protein level in HCT116 and RKO cells transfected with HA-RAB7A plasmids for 48 h. (E-F) HCT116 and RKO cells were transiently transfected with mRFP-GFP-LC3 and HA-RAB7A, then treated with OXI (30 μM). Scale bars, 10 μm. (G) Quantification of the ratio of yellow puncta (autophagosome) versus red puncta (autolysosome). (H) The expression level of RAB7A in xenografts was evaluated by IHC. Scale bar: 50 μm. All data are means ± SD.

    Journal: International Journal of Biological Sciences

    Article Title: Repurposing Oxiconazole against Colorectal Cancer via PRDX2-mediated Autophagy Arrest

    doi: 10.7150/ijbs.70679

    Figure Lengend Snippet: OXI inhibits autophagosome-lysosome fusion by repressing PRDX2/RAB7A axis in CRC cells. (A) RKO and HCT116 cells were treated with OXI (30 μM) or RAPA (100 nM) for 24 h, and then dyed with LysoTracker (75 nM) for 30 min. (B) Western blotting analysis of Myosin, SNAP29, Dynactin, Dynein HC and RAB7A treated with or without OXI (30 μM). (C) Western blotting analysis of RAB7A in HCT116 and RKO cells transfected with Vector or HA-PRDX2 and treated with OXI (30 μM). (D) Western blotting analysis of RAB7A protein level in HCT116 and RKO cells transfected with HA-RAB7A plasmids for 48 h. (E-F) HCT116 and RKO cells were transiently transfected with mRFP-GFP-LC3 and HA-RAB7A, then treated with OXI (30 μM). Scale bars, 10 μm. (G) Quantification of the ratio of yellow puncta (autophagosome) versus red puncta (autolysosome). (H) The expression level of RAB7A in xenografts was evaluated by IHC. Scale bar: 50 μm. All data are means ± SD.

    Article Snippet: The antibodies were as follows: LC3B (NB100-233) was obtained from Novus Biologicals (Saint Charles, MO, USA); anti-ATG5 (12994S), anti-Beclin1 (3738), anti-Bcl-2 (15071), anti-p-Akt (4060S), anti-Akt (4685S), anti-mTOR (2972S), anti-p-mTOR (2971S), anti-p70S6K (9202S), anti-p-p70S6K (9208S), anti-p-4EBP1 (9451S), anti-caspase 3 (9662S), anti-4EBP1 (9452S) and anti-cleaved-caspase 3 (9664S) were purchased from Cell Signaling Technology; anti-PRDX2 (ab109367) and anti-Ki67 (ab16667) were obtained from Abcam; anti-β-actin (sc-1616), anti-p62 (sc-48402), anti-SNAP29 (sc-135564), anti-Myosin (sc-393053), anti-Dynactin (sc-365274), anti-Dynein HC (sc-514579) and anti-RAB7A (sc-376362) were purchased from Santa Cruz Biotechnology.

    Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing, Immunohistochemistry