Structured Review

Santa Cruz Biotechnology anti cygb
The expression of <t>Cygb</t> in HI animals injected with shRNA or cDNA. The expression of Cygb mRNA at 18 h post-HI was confirmed by RT-PCR ( A ). The expression of CYGB protein at 24 h post-HI was confirmed by Western blotting using anti-CYGB antibody ( C ). <t>β-Actin</t>
Anti Cygb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cygb/product/Santa Cruz Biotechnology
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
anti cygb - by Bioz Stars, 2020-08
90/100 stars

Images

1) Product Images from "Mechanisms of Neuroprotection from Hypoxia-Ischemia (HI) Brain Injury by Up-regulation of Cytoglobin (CYGB) in a Neonatal Rat Model *"

Article Title: Mechanisms of Neuroprotection from Hypoxia-Ischemia (HI) Brain Injury by Up-regulation of Cytoglobin (CYGB) in a Neonatal Rat Model *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.428789

The expression of Cygb in HI animals injected with shRNA or cDNA. The expression of Cygb mRNA at 18 h post-HI was confirmed by RT-PCR ( A ). The expression of CYGB protein at 24 h post-HI was confirmed by Western blotting using anti-CYGB antibody ( C ). β-Actin
Figure Legend Snippet: The expression of Cygb in HI animals injected with shRNA or cDNA. The expression of Cygb mRNA at 18 h post-HI was confirmed by RT-PCR ( A ). The expression of CYGB protein at 24 h post-HI was confirmed by Western blotting using anti-CYGB antibody ( C ). β-Actin

Techniques Used: Expressing, Injection, shRNA, Reverse Transcription Polymerase Chain Reaction, Western Blot

Infection of neonatal brain with adenovirus carrying Cygb cDNA or shRNA. CYGB expression at various time points was confirmed by Western blotting using anti-CYGB antibody. β-actin was used as an internal control. A and C , representative electrophoretic
Figure Legend Snippet: Infection of neonatal brain with adenovirus carrying Cygb cDNA or shRNA. CYGB expression at various time points was confirmed by Western blotting using anti-CYGB antibody. β-actin was used as an internal control. A and C , representative electrophoretic

Techniques Used: Infection, shRNA, Expressing, Western Blot

2) Product Images from "Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro"

Article Title: Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro

Journal: Cytotechnology

doi: 10.1007/s10616-016-0047-2

Western blot analysis of Cygb, PPARγ, CEBPα and FABP4. during differentiation process. a Western blot results of Cygb, PPARγ, CEBPα and FABP4 during differentiation process, b quantitative results of western blot band intensities. * P
Figure Legend Snippet: Western blot analysis of Cygb, PPARγ, CEBPα and FABP4. during differentiation process. a Western blot results of Cygb, PPARγ, CEBPα and FABP4 during differentiation process, b quantitative results of western blot band intensities. * P

Techniques Used: Western Blot

Cygb overexpression increases adipogenic differentiation. a Structure of Cygb gene construct, b relative mRNA expression level of Cygb in transduced cells, c oil red staining of differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells, d gene expression levels of PPARγ, CEBPα and FABP4 in differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells at the end of 8 day differentiation period. * P
Figure Legend Snippet: Cygb overexpression increases adipogenic differentiation. a Structure of Cygb gene construct, b relative mRNA expression level of Cygb in transduced cells, c oil red staining of differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells, d gene expression levels of PPARγ, CEBPα and FABP4 in differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells at the end of 8 day differentiation period. * P

Techniques Used: Over Expression, Construct, Expressing, Staining

Gene expression analysis of Cygb, PPARγ, CEBPα and FABP4 during differentiation process. Indicated genes showed an increased in relative mRNA expression in a time dependent manner. * P
Figure Legend Snippet: Gene expression analysis of Cygb, PPARγ, CEBPα and FABP4 during differentiation process. Indicated genes showed an increased in relative mRNA expression in a time dependent manner. * P

Techniques Used: Expressing

Immunocytochemistry analysis of PPARγ, CEBPα and FABP4 during differentiation process. PPARγ, CEBPα and FABP4 staining at day 0 and day 8 is shown. Expression of PPARγ, CEBPα and FABP4 increased during differentiation process. DAPI: Nuclei staining, FITC: Cygb staining. Scale bar 10 µm
Figure Legend Snippet: Immunocytochemistry analysis of PPARγ, CEBPα and FABP4 during differentiation process. PPARγ, CEBPα and FABP4 staining at day 0 and day 8 is shown. Expression of PPARγ, CEBPα and FABP4 increased during differentiation process. DAPI: Nuclei staining, FITC: Cygb staining. Scale bar 10 µm

Techniques Used: Immunocytochemistry, Staining, Expressing

Related Articles

Incubation:

Article Title: Mechanisms of Neuroprotection from Hypoxia-Ischemia (HI) Brain Injury by Up-regulation of Cytoglobin (CYGB) in a Neonatal Rat Model *
Article Snippet: .. The membrane was blocked by a 1-h incubation at room temperature in a Tris-buffered saline solution (TBS-T; 20 m m Tris, pH 7.6, 135 m m NaCl, and 0.05% Tween) containing 5% nonfat dry milk and then incubated with different primary antibodies, including anti-CYGB (1:400 dilution; Santa Cruz Biotechnology, Inc.), anti-β-actin (1:1000 dilution; CST) overnight at 4 °C. .. After washing the membrane three times with TBS-T, the secondary antibody HRP-labeled goat anti-rabbit IgG was then added to the membrane according to the vendor's recommendation (1:8000 dilution; CST) and incubated for 1 h at room temperature and then washed again as described previously.

Article Title: Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro
Article Snippet: .. Cells were incubated with the following primary antibodies; anti-PPARγ (1:200 dilution; MA5-14889; Sigma), anti-CEBPα (1:400 dilution; 8178; Cell Signaling Technology, Beverly, MA, USA), anti-FABP4 (1:100 dilution; sc-271529; Santa Cruz Biotechnology), and anti-Cygb (1:200 dilution; sc-66855; Santa Cruz Biotechnology) overnight at 4 °C. .. Cells were then treated with AlexaFluor 488 FITC conjugated goat anti-rabbit or anti-mouse IgG (1:200 dilution; ThermoFisher, Waltham, MA, USA) secondary antibodies for 1 h at room temperature.

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    Santa Cruz Biotechnology anti cygb
    The expression of <t>Cygb</t> in HI animals injected with shRNA or cDNA. The expression of Cygb mRNA at 18 h post-HI was confirmed by RT-PCR ( A ). The expression of CYGB protein at 24 h post-HI was confirmed by Western blotting using anti-CYGB antibody ( C ). <t>β-Actin</t>
    Anti Cygb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cygb/product/Santa Cruz Biotechnology
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti cygb - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology rabbit anti cygb
    Western blot analysis of <t>Cygb</t> and <t>nNOS</t> in dorsal hippocampus after CRS. Representative blots are shown. Graphs represent the statistical densitometric analyses of data from three separate experiments. Data are expressed as mean group values+SEM of FRL control. Two-way ANOVA analysis revealed differences between FRL versus FSL (*p
    Rabbit Anti Cygb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cygb/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cygb - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    The expression of Cygb in HI animals injected with shRNA or cDNA. The expression of Cygb mRNA at 18 h post-HI was confirmed by RT-PCR ( A ). The expression of CYGB protein at 24 h post-HI was confirmed by Western blotting using anti-CYGB antibody ( C ). β-Actin

    Journal: The Journal of Biological Chemistry

    Article Title: Mechanisms of Neuroprotection from Hypoxia-Ischemia (HI) Brain Injury by Up-regulation of Cytoglobin (CYGB) in a Neonatal Rat Model *

    doi: 10.1074/jbc.M112.428789

    Figure Lengend Snippet: The expression of Cygb in HI animals injected with shRNA or cDNA. The expression of Cygb mRNA at 18 h post-HI was confirmed by RT-PCR ( A ). The expression of CYGB protein at 24 h post-HI was confirmed by Western blotting using anti-CYGB antibody ( C ). β-Actin

    Article Snippet: The membrane was blocked by a 1-h incubation at room temperature in a Tris-buffered saline solution (TBS-T; 20 m m Tris, pH 7.6, 135 m m NaCl, and 0.05% Tween) containing 5% nonfat dry milk and then incubated with different primary antibodies, including anti-CYGB (1:400 dilution; Santa Cruz Biotechnology, Inc.), anti-β-actin (1:1000 dilution; CST) overnight at 4 °C.

    Techniques: Expressing, Injection, shRNA, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Infection of neonatal brain with adenovirus carrying Cygb cDNA or shRNA. CYGB expression at various time points was confirmed by Western blotting using anti-CYGB antibody. β-actin was used as an internal control. A and C , representative electrophoretic

    Journal: The Journal of Biological Chemistry

    Article Title: Mechanisms of Neuroprotection from Hypoxia-Ischemia (HI) Brain Injury by Up-regulation of Cytoglobin (CYGB) in a Neonatal Rat Model *

    doi: 10.1074/jbc.M112.428789

    Figure Lengend Snippet: Infection of neonatal brain with adenovirus carrying Cygb cDNA or shRNA. CYGB expression at various time points was confirmed by Western blotting using anti-CYGB antibody. β-actin was used as an internal control. A and C , representative electrophoretic

    Article Snippet: The membrane was blocked by a 1-h incubation at room temperature in a Tris-buffered saline solution (TBS-T; 20 m m Tris, pH 7.6, 135 m m NaCl, and 0.05% Tween) containing 5% nonfat dry milk and then incubated with different primary antibodies, including anti-CYGB (1:400 dilution; Santa Cruz Biotechnology, Inc.), anti-β-actin (1:1000 dilution; CST) overnight at 4 °C.

    Techniques: Infection, shRNA, Expressing, Western Blot

    Western blot analysis of Cygb, PPARγ, CEBPα and FABP4. during differentiation process. a Western blot results of Cygb, PPARγ, CEBPα and FABP4 during differentiation process, b quantitative results of western blot band intensities. * P

    Journal: Cytotechnology

    Article Title: Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro

    doi: 10.1007/s10616-016-0047-2

    Figure Lengend Snippet: Western blot analysis of Cygb, PPARγ, CEBPα and FABP4. during differentiation process. a Western blot results of Cygb, PPARγ, CEBPα and FABP4 during differentiation process, b quantitative results of western blot band intensities. * P

    Article Snippet: Cells were incubated with the following primary antibodies; anti-PPARγ (1:200 dilution; MA5-14889; Sigma), anti-CEBPα (1:400 dilution; 8178; Cell Signaling Technology, Beverly, MA, USA), anti-FABP4 (1:100 dilution; sc-271529; Santa Cruz Biotechnology), and anti-Cygb (1:200 dilution; sc-66855; Santa Cruz Biotechnology) overnight at 4 °C.

    Techniques: Western Blot

    Cygb overexpression increases adipogenic differentiation. a Structure of Cygb gene construct, b relative mRNA expression level of Cygb in transduced cells, c oil red staining of differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells, d gene expression levels of PPARγ, CEBPα and FABP4 in differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells at the end of 8 day differentiation period. * P

    Journal: Cytotechnology

    Article Title: Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro

    doi: 10.1007/s10616-016-0047-2

    Figure Lengend Snippet: Cygb overexpression increases adipogenic differentiation. a Structure of Cygb gene construct, b relative mRNA expression level of Cygb in transduced cells, c oil red staining of differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells, d gene expression levels of PPARγ, CEBPα and FABP4 in differentiated 3T3-F442A–GFP and 3T3-F442A–Cygb cells at the end of 8 day differentiation period. * P

    Article Snippet: Cells were incubated with the following primary antibodies; anti-PPARγ (1:200 dilution; MA5-14889; Sigma), anti-CEBPα (1:400 dilution; 8178; Cell Signaling Technology, Beverly, MA, USA), anti-FABP4 (1:100 dilution; sc-271529; Santa Cruz Biotechnology), and anti-Cygb (1:200 dilution; sc-66855; Santa Cruz Biotechnology) overnight at 4 °C.

    Techniques: Over Expression, Construct, Expressing, Staining

    Gene expression analysis of Cygb, PPARγ, CEBPα and FABP4 during differentiation process. Indicated genes showed an increased in relative mRNA expression in a time dependent manner. * P

    Journal: Cytotechnology

    Article Title: Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro

    doi: 10.1007/s10616-016-0047-2

    Figure Lengend Snippet: Gene expression analysis of Cygb, PPARγ, CEBPα and FABP4 during differentiation process. Indicated genes showed an increased in relative mRNA expression in a time dependent manner. * P

    Article Snippet: Cells were incubated with the following primary antibodies; anti-PPARγ (1:200 dilution; MA5-14889; Sigma), anti-CEBPα (1:400 dilution; 8178; Cell Signaling Technology, Beverly, MA, USA), anti-FABP4 (1:100 dilution; sc-271529; Santa Cruz Biotechnology), and anti-Cygb (1:200 dilution; sc-66855; Santa Cruz Biotechnology) overnight at 4 °C.

    Techniques: Expressing

    Immunocytochemistry analysis of PPARγ, CEBPα and FABP4 during differentiation process. PPARγ, CEBPα and FABP4 staining at day 0 and day 8 is shown. Expression of PPARγ, CEBPα and FABP4 increased during differentiation process. DAPI: Nuclei staining, FITC: Cygb staining. Scale bar 10 µm

    Journal: Cytotechnology

    Article Title: Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro

    doi: 10.1007/s10616-016-0047-2

    Figure Lengend Snippet: Immunocytochemistry analysis of PPARγ, CEBPα and FABP4 during differentiation process. PPARγ, CEBPα and FABP4 staining at day 0 and day 8 is shown. Expression of PPARγ, CEBPα and FABP4 increased during differentiation process. DAPI: Nuclei staining, FITC: Cygb staining. Scale bar 10 µm

    Article Snippet: Cells were incubated with the following primary antibodies; anti-PPARγ (1:200 dilution; MA5-14889; Sigma), anti-CEBPα (1:400 dilution; 8178; Cell Signaling Technology, Beverly, MA, USA), anti-FABP4 (1:100 dilution; sc-271529; Santa Cruz Biotechnology), and anti-Cygb (1:200 dilution; sc-66855; Santa Cruz Biotechnology) overnight at 4 °C.

    Techniques: Immunocytochemistry, Staining, Expressing

    Expression and methylation of CYGB in breast cancer. a Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of CYGB mRNA expression in breast tumor tissue samples and paired tumor adjacent tissue samples. b Low CYGB expression is correlated with poor ten-year relapse-free survival (RFS) in breast cancer patients. Prognosis data was acquired and analyzed using the Kaplan-Meier Plotter database ( www.kmplot.com/analysis/index. php?p=service cancer=breast ). c CYGB mRNA expression and promoter methylation in paired breast cancer tissue samples and non-cancerous breast tissue samples of 36 patients from The Cancer Genome Atlas (TCGA). The data were accessed through cBioPortal ( http://www.cbioportal.org /). d RT-PCR and MSP analyses of CYGB mRNA expression and promoter methylation in breast cancer cell lines. Non-tumorigenic mammary epithelial cell lines MCF10A and HMEC as well as normal breast tissue samples were used as controls. GAPDH was detected as an input control. e RT-PCR and MSP indicate demethylation by Aza and TSA (A + T) restored CYGB expression in BT549, MB231, MCF7 cells. GAPDH was detected as an input control. Aza: 5-aza-2′-deoxycytidine; BN: breast normal tissue; BrCa: breast cancer tissue; M: methylated; MSP: methylation-specific polymerase chain reaction; RT-PCR: reverse transcription-polymerase chain reaction; U: unmethylated

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism

    doi: 10.1186/s13046-018-0979-9

    Figure Lengend Snippet: Expression and methylation of CYGB in breast cancer. a Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of CYGB mRNA expression in breast tumor tissue samples and paired tumor adjacent tissue samples. b Low CYGB expression is correlated with poor ten-year relapse-free survival (RFS) in breast cancer patients. Prognosis data was acquired and analyzed using the Kaplan-Meier Plotter database ( www.kmplot.com/analysis/index. php?p=service cancer=breast ). c CYGB mRNA expression and promoter methylation in paired breast cancer tissue samples and non-cancerous breast tissue samples of 36 patients from The Cancer Genome Atlas (TCGA). The data were accessed through cBioPortal ( http://www.cbioportal.org /). d RT-PCR and MSP analyses of CYGB mRNA expression and promoter methylation in breast cancer cell lines. Non-tumorigenic mammary epithelial cell lines MCF10A and HMEC as well as normal breast tissue samples were used as controls. GAPDH was detected as an input control. e RT-PCR and MSP indicate demethylation by Aza and TSA (A + T) restored CYGB expression in BT549, MB231, MCF7 cells. GAPDH was detected as an input control. Aza: 5-aza-2′-deoxycytidine; BN: breast normal tissue; BrCa: breast cancer tissue; M: methylated; MSP: methylation-specific polymerase chain reaction; RT-PCR: reverse transcription-polymerase chain reaction; U: unmethylated

    Article Snippet: The following primary antibodies were used in this study: CYGB (Santa Cruz Biotechnology, sc-365,246), GAPDH (Cell Signaling Technology, #2118), p53 (Santa Cruz Biotechnology, sc-126), p21 (Cell Signaling Technology, #2947), β-actin (Abcam, ab8226), GLUT1 (Santa Cruz Biotechnology, sc-377,228), HXK2 (Santa Cruz Biotechnology, sc-374,091), TIGAR (Santa Cruz Biotechnology, sc-166,290).

    Techniques: Expressing, Methylation, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Polymerase Chain Reaction

    Western blot analysis of Cygb and nNOS in dorsal hippocampus after CRS. Representative blots are shown. Graphs represent the statistical densitometric analyses of data from three separate experiments. Data are expressed as mean group values+SEM of FRL control. Two-way ANOVA analysis revealed differences between FRL versus FSL (*p

    Journal: PLoS ONE

    Article Title: A Gene-Environment Study of Cytoglobin in the Human and Rat Hippocampus

    doi: 10.1371/journal.pone.0063288

    Figure Lengend Snippet: Western blot analysis of Cygb and nNOS in dorsal hippocampus after CRS. Representative blots are shown. Graphs represent the statistical densitometric analyses of data from three separate experiments. Data are expressed as mean group values+SEM of FRL control. Two-way ANOVA analysis revealed differences between FRL versus FSL (*p

    Article Snippet: The following antibodies were used: rabbit anti-Cygb 1∶1000 (5092/6, in house), rabbit anti-nNOS 1∶500 (Santa Cruz, sc-546) and mouse anti-GAPDH 1∶2000 (Covance) followed by the appropriate HPP-conjugated secondary antibodies: anti-rabbit antibody (1∶10,000) or anti-mouse antibody (1∶2,000) (both obtained from Pierce, IL, USA).

    Techniques: Western Blot

    Western blot analysis of Cygb and nNOS in ventral hippocampus after CRS. Western blot analysis of Cygb and nNOS in ventral hippocampus after CRS. Representative blots are shown. Graphs represent the statistical densitometric analyses of data from three separate experiments. Data are expressed as mean group values+SEM of FRL control. Two-way ANOVA analysis revealed differences a significant effect of CRS in the FSL (*p

    Journal: PLoS ONE

    Article Title: A Gene-Environment Study of Cytoglobin in the Human and Rat Hippocampus

    doi: 10.1371/journal.pone.0063288

    Figure Lengend Snippet: Western blot analysis of Cygb and nNOS in ventral hippocampus after CRS. Western blot analysis of Cygb and nNOS in ventral hippocampus after CRS. Representative blots are shown. Graphs represent the statistical densitometric analyses of data from three separate experiments. Data are expressed as mean group values+SEM of FRL control. Two-way ANOVA analysis revealed differences a significant effect of CRS in the FSL (*p

    Article Snippet: The following antibodies were used: rabbit anti-Cygb 1∶1000 (5092/6, in house), rabbit anti-nNOS 1∶500 (Santa Cruz, sc-546) and mouse anti-GAPDH 1∶2000 (Covance) followed by the appropriate HPP-conjugated secondary antibodies: anti-rabbit antibody (1∶10,000) or anti-mouse antibody (1∶2,000) (both obtained from Pierce, IL, USA).

    Techniques: Western Blot

    mRNA expression of Cygb and nNOS. mRNA expression of Cygb and nNOS in the dorsal and ventral hippocampus after chronic restraint stress (CRS) using real-time qPCR. Values for each individual were normalized with the geometric mean of the reference genes CycA/Actb and CycA/Hprt1 for the dorsal and ventral hippocampus, respectively. Plotted data show mean group values+SEM of mRNA expression as % of FRL control. Differences between groups were analyzed using two-way ANOVA (*p

    Journal: PLoS ONE

    Article Title: A Gene-Environment Study of Cytoglobin in the Human and Rat Hippocampus

    doi: 10.1371/journal.pone.0063288

    Figure Lengend Snippet: mRNA expression of Cygb and nNOS. mRNA expression of Cygb and nNOS in the dorsal and ventral hippocampus after chronic restraint stress (CRS) using real-time qPCR. Values for each individual were normalized with the geometric mean of the reference genes CycA/Actb and CycA/Hprt1 for the dorsal and ventral hippocampus, respectively. Plotted data show mean group values+SEM of mRNA expression as % of FRL control. Differences between groups were analyzed using two-way ANOVA (*p

    Article Snippet: The following antibodies were used: rabbit anti-Cygb 1∶1000 (5092/6, in house), rabbit anti-nNOS 1∶500 (Santa Cruz, sc-546) and mouse anti-GAPDH 1∶2000 (Covance) followed by the appropriate HPP-conjugated secondary antibodies: anti-rabbit antibody (1∶10,000) or anti-mouse antibody (1∶2,000) (both obtained from Pierce, IL, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Overview of Cygb and nNOS expression and co-expression in the hippocampus. A shows an overview of Cygb-IR (red) in the rat hippocampus. Red, green and blue squares are shown in higher magnification in figure 4 . B shows an overview of nNOS-IR (green) expression and C is the merged image of A and B. Abbreviations: Cornu ammonis1–3 (CA1–3), dentate gyrus (DG).

    Journal: PLoS ONE

    Article Title: A Gene-Environment Study of Cytoglobin in the Human and Rat Hippocampus

    doi: 10.1371/journal.pone.0063288

    Figure Lengend Snippet: Overview of Cygb and nNOS expression and co-expression in the hippocampus. A shows an overview of Cygb-IR (red) in the rat hippocampus. Red, green and blue squares are shown in higher magnification in figure 4 . B shows an overview of nNOS-IR (green) expression and C is the merged image of A and B. Abbreviations: Cornu ammonis1–3 (CA1–3), dentate gyrus (DG).

    Article Snippet: The following antibodies were used: rabbit anti-Cygb 1∶1000 (5092/6, in house), rabbit anti-nNOS 1∶500 (Santa Cruz, sc-546) and mouse anti-GAPDH 1∶2000 (Covance) followed by the appropriate HPP-conjugated secondary antibodies: anti-rabbit antibody (1∶10,000) or anti-mouse antibody (1∶2,000) (both obtained from Pierce, IL, USA).

    Techniques: Expressing

    Validation of the immunohistochemistry method. A and B shows Cygb-IR (red) and nNOS-IR (green), respectively, in a coronal section of the rat pituitary gland. C is the merged image of A and B . Note complete separation of the red and green signal showing no false cross reactivity from the secondary antibodies or artifact due to problematic filter settings of the microscope. Scale bar 50 µm.

    Journal: PLoS ONE

    Article Title: A Gene-Environment Study of Cytoglobin in the Human and Rat Hippocampus

    doi: 10.1371/journal.pone.0063288

    Figure Lengend Snippet: Validation of the immunohistochemistry method. A and B shows Cygb-IR (red) and nNOS-IR (green), respectively, in a coronal section of the rat pituitary gland. C is the merged image of A and B . Note complete separation of the red and green signal showing no false cross reactivity from the secondary antibodies or artifact due to problematic filter settings of the microscope. Scale bar 50 µm.

    Article Snippet: The following antibodies were used: rabbit anti-Cygb 1∶1000 (5092/6, in house), rabbit anti-nNOS 1∶500 (Santa Cruz, sc-546) and mouse anti-GAPDH 1∶2000 (Covance) followed by the appropriate HPP-conjugated secondary antibodies: anti-rabbit antibody (1∶10,000) or anti-mouse antibody (1∶2,000) (both obtained from Pierce, IL, USA).

    Techniques: Immunohistochemistry, Microscopy

    Analysis of Cygb and nNOS co-localization. A–C shows high-resolution stacks of the areas from figure 3 . A Cygb-IR (red) and nNOS-IR (green) in the CA1. Yellow arrowheads denote neurons with co-localized Cygb-IR and nNOS-IR. B shows the stratum radiatum (Rad). Note most nNOS-IR fibers do not co-express Cygb-IR. C shows the dente gyrus (DG). A1–C1 shows calculated co-localization (white). Scale bar 50 µm.

    Journal: PLoS ONE

    Article Title: A Gene-Environment Study of Cytoglobin in the Human and Rat Hippocampus

    doi: 10.1371/journal.pone.0063288

    Figure Lengend Snippet: Analysis of Cygb and nNOS co-localization. A–C shows high-resolution stacks of the areas from figure 3 . A Cygb-IR (red) and nNOS-IR (green) in the CA1. Yellow arrowheads denote neurons with co-localized Cygb-IR and nNOS-IR. B shows the stratum radiatum (Rad). Note most nNOS-IR fibers do not co-express Cygb-IR. C shows the dente gyrus (DG). A1–C1 shows calculated co-localization (white). Scale bar 50 µm.

    Article Snippet: The following antibodies were used: rabbit anti-Cygb 1∶1000 (5092/6, in house), rabbit anti-nNOS 1∶500 (Santa Cruz, sc-546) and mouse anti-GAPDH 1∶2000 (Covance) followed by the appropriate HPP-conjugated secondary antibodies: anti-rabbit antibody (1∶10,000) or anti-mouse antibody (1∶2,000) (both obtained from Pierce, IL, USA).

    Techniques: