anti cyclind1 antibody  (Thermo Fisher)


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    Name:
    Cyclin D1 Monoclonal Antibody DCS 6
    Description:
    Cyclin D1 Monoclonal Antibody for Western Blot IF IHC Flow IP IA
    Catalog Number:
    AHF0082
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Cell Analysis|Cell Cycle|Cellular Imaging|Flow Cytometry Antibodies & Secondary Detection|Flow Cytometry Antibodies for Cell Viability, Proliferation & Function|Flow Cytometry Cell Cycle Assays|Flow Cytometry of Cellular Processes|IHC Staining & Detection|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Immunohistochemistry (IHC)|Immunoprecipitation|Protein Assays and Analysis|Protein Biology|Protein Purification & Isolation|Western Blot Detection|Western Blotting|Flow Cytometry|Cell Viability, Proliferation & Function
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    Structured Review

    Thermo Fisher anti cyclind1 antibody
    Effects of VPA exposure in utero on the amount of cell cycle regulatory proteins and total acetylated histone H3 protein in the embryonic cerebral walls. A , Immunoblot analysis of <t>cyclinD1,</t> cyclin-dependent kinase (cdk) 2, cdk4, and p27 Kip1 in cerebral walls on E12. B , The amount of each protein in controls was considered to be 100%. C , The amount of total acetylated histone H3 protein in cerebral walls on E12. * p
    Cyclin D1 Monoclonal Antibody for Western Blot IF IHC Flow IP IA
    https://www.bioz.com/result/anti cyclind1 antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cyclind1 antibody - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "In Utero Exposure to Valproic Acid Induces Neocortical Dysgenesis via Dysregulation of Neural Progenitor Cell Proliferation/Differentiation"

    Article Title: In Utero Exposure to Valproic Acid Induces Neocortical Dysgenesis via Dysregulation of Neural Progenitor Cell Proliferation/Differentiation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0229-16.2016

    Effects of VPA exposure in utero on the amount of cell cycle regulatory proteins and total acetylated histone H3 protein in the embryonic cerebral walls. A , Immunoblot analysis of cyclinD1, cyclin-dependent kinase (cdk) 2, cdk4, and p27 Kip1 in cerebral walls on E12. B , The amount of each protein in controls was considered to be 100%. C , The amount of total acetylated histone H3 protein in cerebral walls on E12. * p
    Figure Legend Snippet: Effects of VPA exposure in utero on the amount of cell cycle regulatory proteins and total acetylated histone H3 protein in the embryonic cerebral walls. A , Immunoblot analysis of cyclinD1, cyclin-dependent kinase (cdk) 2, cdk4, and p27 Kip1 in cerebral walls on E12. B , The amount of each protein in controls was considered to be 100%. C , The amount of total acetylated histone H3 protein in cerebral walls on E12. * p

    Techniques Used: In Utero

    Related Articles

    SDS Page:

    Article Title: CDK4 T172 Phosphorylation Is Central in a CDK7-Dependent Bidirectional CDK4/CDK2 Interplay Mediated by p21 Phosphorylation at the Restriction Point
    Article Snippet: After addition of 1 mM ATP, the suspensions were incubated at 30°C for 30 min. After three washes in CAK buffer and three washes in pRb kinase buffer, the immunoprecipitated proteins were assayed for pRb kinase activity as described above. .. Immunoblot analysis SDS-PAGE separations of equal amounts of whole cell extract proteins , and immunoprecipitates were immunodetected using the following antibodies: monoclonal antibodies against cyclin D1 (DCS-6), cyclin D3 (DCS-22), p27 (DCS-72), p21 (DCS60) (all from Neomarkers); monoclonal antibody anti-cyclin E (HE12) (Pierce Biotechnology); monoclonal anti-cyclin A E43.2 (kind gift from Tim Hunt); anti-total pRb monoclonal antibody (#554136, BD Pharmingen); polyclonal anti-phospho-pRb antibodies (T826 and T821) (Biosource-Invitrogen); polyclonal antibodies anti-phospho-pRb (S807/811), anti-phospho-CDK2 (T160), anti-phospho-histone H3 (S10) (all from Cell Signaling Technology); monoclonal antibody against HA epitope (F7), CDK7 (C-4) and MAT1 (F-6), polyclonal antibodies against CDK4 (C-22), CDK6 (C-21), CDK2 (M-2), cyclin H (C-18), phospho-CDK7 (T170), XPD (TFIIH p80) (all from Santa Cruz Biotechnology); monoclonal antibodies against total RNA Polymerase II (8WG16), phospho-Ser2 (H5) or phospho-Ser5 (H14) of RNA Polymerase II (all from Covance). .. Secondary antibodies were either coupled to horseradish peroxidase (Amersham Biosciences) for detection by enhanced chemiluminescence (Western Lightning, Perkin-Elmer) or to DyLight 680 and 800 (Pierce Biotechnology) for infrared fluorescence detection using the Odyssey scanner (LI-COR).

    Blocking Assay:

    Article Title: AT7867 is a potent and oral inhibitor of AKT and p70 S6 kinase that induces pharmacodynamic changes and inhibits human tumor xenograft growth
    Article Snippet: Samples were separated by SDS-PAGE and transferred to 0.45 μm Immobilon-P transfer membrane (Millipore, Watford, Herts, UK). .. The membranes were blocked for 1 hour in blocking solution (5% milk in TBST) before incubating at 4°C, shaking overnight in the following antibodies diluted in blocking solution: AKT, phospho-AKT (Ser473), phospho-GSK3β (Ser9), phospho-S6RP (Ser240/244), FKHR, cleaved PARP (Asp214) all at 1:1000, S6 ribosomal protein at 1:2000, phospho-S6RP (Ser235/236) at 1:10,000, phospho-FKHR (Thr24)/FKHRL1 (Thr32) at 1:500 (all purchased from Cell Signaling Technology), GSK3β at 1:1000 (BD Biosciences, Oxford, UK), cyclin D1 Ab-1 (DCS-6) at 1:1000 (NeoMarkers, Lab Vision, Freemont, CA, USA) and GAPDH at 1:2×106 (Chemicon International, Millipore, Watford, Herts, UK). .. The membranes were washed before incubation in goat anti-rabbit (1:1000 or 1:5000) or anti-mouse (1:10,000) HRP-conjugated secondary antibody for 1 hour at room temperature.

    Incubation:

    Article Title: Different doses of partial liver irradiation promotes hepatic regeneration in rat
    Article Snippet: The membranes were blocked with TBS-0.05% Tween 20 (TBST) containing 5% non-fat milk to block nonspecific binding sites for 2 h at room temperature. .. Then, the membranes were incubated with anti-cyclinD1 (Pierce Biotechnology, Rockford, IL, USA), and anti-β-actin (1:1000, Cell Signaling Technology, Boston, MA) overnight at 4°C. .. The membranes were washed with TBST solution thrice and incubated with anti-mouse IgG (1:1000, Beijing Applygen Technologies, Inc., Beijing, China) conjugated for 2 h at room temperature.

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    Thermo Fisher anti cyclind1 antibody
    Effects of VPA exposure in utero on the amount of cell cycle regulatory proteins and total acetylated histone H3 protein in the embryonic cerebral walls. A , Immunoblot analysis of <t>cyclinD1,</t> cyclin-dependent kinase (cdk) 2, cdk4, and p27 Kip1 in cerebral walls on E12. B , The amount of each protein in controls was considered to be 100%. C , The amount of total acetylated histone H3 protein in cerebral walls on E12. * p
    Anti Cyclind1 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclind1 antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cyclind1 antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher anti cyclin d1 antibodies
    Macrophages promote cell proliferation of normal prostate epithelial cells. ( A ) A diagram for demonstrating cell co-cultivation of normal prostate epithelial PZ-HPV-7 cells (blue area) and Raw 264.7 cells (purple area). Cells were plated on matrigel in µ-Slides (Ibidi) and overlaid with KSF media. ( B ) PZ-HPV-7 cells grown on matrigel in 3D with or without Raw264.7 cells in a co-culture system (described in A) were fixed and immuno-stained with <t>cyclin</t> D1 (green). DAPI (blue) was used to visualize all cell nuclei. Scale bar: 20 µm. ( C ) Cell proliferation index of PZ-HPV-7 cells that were cultured with or without Raw 264.7 cells in 3D was quantified. *p
    Anti Cyclin D1 Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1 antibodies/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cyclin d1 antibodies - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effects of VPA exposure in utero on the amount of cell cycle regulatory proteins and total acetylated histone H3 protein in the embryonic cerebral walls. A , Immunoblot analysis of cyclinD1, cyclin-dependent kinase (cdk) 2, cdk4, and p27 Kip1 in cerebral walls on E12. B , The amount of each protein in controls was considered to be 100%. C , The amount of total acetylated histone H3 protein in cerebral walls on E12. * p

    Journal: The Journal of Neuroscience

    Article Title: In Utero Exposure to Valproic Acid Induces Neocortical Dysgenesis via Dysregulation of Neural Progenitor Cell Proliferation/Differentiation

    doi: 10.1523/JNEUROSCI.0229-16.2016

    Figure Lengend Snippet: Effects of VPA exposure in utero on the amount of cell cycle regulatory proteins and total acetylated histone H3 protein in the embryonic cerebral walls. A , Immunoblot analysis of cyclinD1, cyclin-dependent kinase (cdk) 2, cdk4, and p27 Kip1 in cerebral walls on E12. B , The amount of each protein in controls was considered to be 100%. C , The amount of total acetylated histone H3 protein in cerebral walls on E12. * p

    Article Snippet: The following antibodies were used for immunoblot analysis: anti-cyclinD1 antibody (1:250; RM-9104, Thermo Fischer Scientific, RRID: AB_720758; 4°C, overnight), anti-cyclin-dependent kinase (cdk) 2 antibody (1:250; sc-163, Santa Cruz Biotechnology, RRID: AB_631215; 4°C, overnight), anti-cdk4 antibody (1:250; sc-260, Santa Cruz Biotechnology, RRID: AB_631219; 4°C, overnight), and anti-p27Kip1 antibody (1:500; 610241, Becton Dickinson, RRID: AB_397636; 4°C, overnight).

    Techniques: In Utero

    Macrophages promote cell proliferation of normal prostate epithelial cells. ( A ) A diagram for demonstrating cell co-cultivation of normal prostate epithelial PZ-HPV-7 cells (blue area) and Raw 264.7 cells (purple area). Cells were plated on matrigel in µ-Slides (Ibidi) and overlaid with KSF media. ( B ) PZ-HPV-7 cells grown on matrigel in 3D with or without Raw264.7 cells in a co-culture system (described in A) were fixed and immuno-stained with cyclin D1 (green). DAPI (blue) was used to visualize all cell nuclei. Scale bar: 20 µm. ( C ) Cell proliferation index of PZ-HPV-7 cells that were cultured with or without Raw 264.7 cells in 3D was quantified. *p

    Journal: Scientific Reports

    Article Title: Macrophage Cytokines Enhance Cell Proliferation of Normal Prostate Epithelial Cells through Activation of ERK and Akt

    doi: 10.1038/s41598-018-26143-8

    Figure Lengend Snippet: Macrophages promote cell proliferation of normal prostate epithelial cells. ( A ) A diagram for demonstrating cell co-cultivation of normal prostate epithelial PZ-HPV-7 cells (blue area) and Raw 264.7 cells (purple area). Cells were plated on matrigel in µ-Slides (Ibidi) and overlaid with KSF media. ( B ) PZ-HPV-7 cells grown on matrigel in 3D with or without Raw264.7 cells in a co-culture system (described in A) were fixed and immuno-stained with cyclin D1 (green). DAPI (blue) was used to visualize all cell nuclei. Scale bar: 20 µm. ( C ) Cell proliferation index of PZ-HPV-7 cells that were cultured with or without Raw 264.7 cells in 3D was quantified. *p

    Article Snippet: Both anti-F4/80 and anti-cyclin D1 antibodies were from Thermo Fisher Scientific; anti-iNOS and anti-CD206 antibodies were obtained from Abcam.

    Techniques: Co-Culture Assay, Staining, Cell Culture

    eHSP90α had a stimulatory effect on HPDE cell anchorage independence but not cell proliferation. (A) Growth curves of HPDE cells treated with control medium (CTRL) or MϕCM for 1 to 5 days in the presence of control IgG or anti-CD91 antibody. Trypan blue exclusion assay was performed to count the numbers of viable cells. The data represent the averages of three independent experiments, and each experiment was performed in triplicate. (B) Growth curves of HPDE cells treated with PBS or 15 μg/ml of rHSP90α for 1 to 5 days. Trypan blue exclusion assay was performed, and the averages of three independent experiments are shown. (C) Flow cytometric analyses of the cell-cycle phase distribution of control medium (CTRL)- vs . MϕCM-treated HPDE cells (upper panel) and PBS- vs . rHSP90α-treated HPDE cells (lower panel). HPDE cells were treated for 24 h and trypsinized for ethanol fixation and propidium iodide staining. The histograph of cell-cycle phases was obtained from 10000 cells analyzed using a FACSCalibur flow cytometer. (D) The levels of cyclin D1, CDK4, Ser-780-phosphorylated Rb, Rb, cyclin E, cyclin A, Tyr-15-phosphorylated CDK2, CDK2, cyclin B, Tyr-15-phosphorylated CDC2, CDC2, and GAPDH in HPDE cells treated with PBS, 15 μg/ml rHSP90α, or 15 μg/ml rHSP90α plus preimmune IgG or anti-CD91 antibody. (E) Soft-agar colony-forming assay of PBS or rHSP90α-treated HPDE cells. HPDE cells (2000 cells seeded on the soft-agar layer in each well of a 6-well plate) were treated with or without 15 μg/ml rHSP90α and refreshed every 3 days for 3 weeks. The colonies were stained with Giemsa and photographed under a microscope (100 ×); only colonies consisting of ≥80 cells were counted. The mean ± SD values of three independent experiments are shown, and each experiment was performed in triplicate. # , P

    Journal: Oncoimmunology

    Article Title: Myeloid-derived macrophages and secreted HSP90α induce pancreatic ductal adenocarcinoma development

    doi: 10.1080/2162402X.2018.1424612

    Figure Lengend Snippet: eHSP90α had a stimulatory effect on HPDE cell anchorage independence but not cell proliferation. (A) Growth curves of HPDE cells treated with control medium (CTRL) or MϕCM for 1 to 5 days in the presence of control IgG or anti-CD91 antibody. Trypan blue exclusion assay was performed to count the numbers of viable cells. The data represent the averages of three independent experiments, and each experiment was performed in triplicate. (B) Growth curves of HPDE cells treated with PBS or 15 μg/ml of rHSP90α for 1 to 5 days. Trypan blue exclusion assay was performed, and the averages of three independent experiments are shown. (C) Flow cytometric analyses of the cell-cycle phase distribution of control medium (CTRL)- vs . MϕCM-treated HPDE cells (upper panel) and PBS- vs . rHSP90α-treated HPDE cells (lower panel). HPDE cells were treated for 24 h and trypsinized for ethanol fixation and propidium iodide staining. The histograph of cell-cycle phases was obtained from 10000 cells analyzed using a FACSCalibur flow cytometer. (D) The levels of cyclin D1, CDK4, Ser-780-phosphorylated Rb, Rb, cyclin E, cyclin A, Tyr-15-phosphorylated CDK2, CDK2, cyclin B, Tyr-15-phosphorylated CDC2, CDC2, and GAPDH in HPDE cells treated with PBS, 15 μg/ml rHSP90α, or 15 μg/ml rHSP90α plus preimmune IgG or anti-CD91 antibody. (E) Soft-agar colony-forming assay of PBS or rHSP90α-treated HPDE cells. HPDE cells (2000 cells seeded on the soft-agar layer in each well of a 6-well plate) were treated with or without 15 μg/ml rHSP90α and refreshed every 3 days for 3 weeks. The colonies were stained with Giemsa and photographed under a microscope (100 ×); only colonies consisting of ≥80 cells were counted. The mean ± SD values of three independent experiments are shown, and each experiment was performed in triplicate. # , P

    Article Snippet: The antibodies used were specific for human STAT3, phosphorylated STAT3 (EMD Millipore, Billerica, MA, USA), HSP90α (AbD Serotec), phosphorylated FAK (Invitrogen), phosphorylated CDK2 (Epitomics, Burlingame, CA, USA), phosphorylated Rb, Rb, phosphorylated CDC2 (Cell Signaling, Danvers, MA, USA), connexin-43 (Sigma, St. Louis, MO, USA), integrin αV (BD Biosciences, San Jose, CA, USA), cyclin D1, MMP-2, MMP-9 (Lab Vision/NeoMarkers Co., Fremont, CA, USA), CDK4, cyclin E, cyclin A, CDK2, cyclin B1, CDC2, FAK, TCF12, Twist-1, Snail, Slug, E-cadherin, connexin-26, fibronectin, and GAPDH (Santa Cruz Biotechnology).

    Techniques: Trypan Blue Exclusion Assay, Flow Cytometry, Staining, Cytometry, Microscopy

    Deletion of 14-3-3ε downregulates cyclin E1 while upregulating cyclin D1 and p27 Kip1 through transcriptional and possibly posttranscriptional mechanisms. (a to i) Western blot analyses were performed on E18.5 hearts. (a) Significant upregulation

    Journal: Molecular and Cellular Biology

    Article Title: 14-3-3? Plays a Role in Cardiac Ventricular Compaction by Regulating the Cardiomyocyte Cell Cycle

    doi: 10.1128/MCB.00829-12

    Figure Lengend Snippet: Deletion of 14-3-3ε downregulates cyclin E1 while upregulating cyclin D1 and p27 Kip1 through transcriptional and possibly posttranscriptional mechanisms. (a to i) Western blot analyses were performed on E18.5 hearts. (a) Significant upregulation

    Article Snippet: Briefly, 30 or 50 μg protein was separated on sodium dodecyl sulfate (SDS)-polyacrylamide gels with a 4 to 15% or 8 to 16% gradient and was electroblotted onto nitrocellulose membranes, blocked with 5% milk, and incubated with primary antibodies against the following proteins at the indicated dilutions: 14-3-3ε (1:200; Santa Cruz Biotechnology), p21Cip1 (1:200; Abcam), p27Kip1 (1:500; BD Biosciences, San Jose, CA), p57Kip2 (1:100; Abcam), cyclin D1 (1:500; Thermo Fisher Scientific), cyclin D3 (1:400; Santa Cruz Biotechnology), Skp2 (1:200; Santa Cruz Biotechnology), p15Ink4b (1:1,000; Cell Signaling Technology), cyclin E1 (1:200; Abcam), Cdk2 (1:400; Santa Cruz Biotechnology), and GAPDH (1:2,000; Cell Signaling Technology).

    Techniques: Western Blot