Journal: EMBO Molecular Medicine

Article Title: CUEDC 2 modulates cardiomyocyte oxidative capacity by regulating GPX 1 stability

doi: 10.15252/emmm.201506010

Figure Lengend Snippet: CUEDC2 protein levels in mouse tissues. Adult (12‐week‐old) mouse was sacrificed and the total protein was extracted from the liver, kidney, lung, spleen, heart, stomach, intestine, prostate, testis, brain, and thymus. (top) In vivo cardiac ischemia/reperfusion model protocol (30‐minute ischemia followed by indicated times of reperfusion). (bottom) Mice were subjected to reversible ischemia in vivo for 30 min and reperfused for different time periods. Protein was extracted from the area at risk of heart at the indicated time points and subjected to immunoblot analysis of CUEDC2 and CUEDC1. Representative results were shown from 2 mice at each time point, and experiments were repeated for three times. Isolated primary rat neonatal cardiomyocytes were subjected to hypoxia with serum‐free medium for 6 h and reoxygenated accompanying adding serum back for different time period in vitro and the protein level of CUEDC2 was tested at different time points during reoxygenation. Each experiment was repeated for three times. CUEDC2 protein level was plotted using the immunohistochemical scores as described in methods. (left) Plot of CUEDC2 scores in each BZ and DZ ( n = 9 patients). (right) Representative images from immunohistochemical staining for CUEDC2 in the tissues from patients who suffered acute myocardial infarctions. The boxed areas in the left images are magnified in the middle and right images. DZ, distant zone; BZ, border zone. Scale bars, 250 μm (left), 50 μm (middle and right). * P = 0.001. Data were shown as mean ± SEM and analyzed by paired t ‐test. Source data are available online for this figure.

Article Snippet: Antibodies used in these studies were as follows: rabbit polyclonal anti‐CUEDC2 antibody (Cat. No. 4839) and rabbit polyclonal anti‐CUEDC1 antibody (Cat. No. 4829) were obtained from ProSci Incorporated.

Techniques: In Vivo, Western Blot, Isolation, In Vitro, Immunohistochemical staining, Staining