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Proteintech anti coq5
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
86/100 stars

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Proteintech coq5
Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/coq5/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
coq5 - by Bioz Stars, 2024-06
86/100 stars

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Structured Review

Proteintech anti coq5
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
86/100 stars

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Proteintech anti coq5
Effect of VA treatment on the levels of CoQ biosynthetic proteins and mRNA levels in the MEFs and tissues of Coq9 R239X mice. ( A-D ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( A ), COQ4 ( B ), <t>COQ5</t> ( C ) and COQ7 ( D ), and the quantitation of the protein bands in MEFs from Coq9 +/+ and Coq9 R239X mice after supplementation with VA at 1 mM ( E-H ) Relative mRNA levels for the genes Coq2 ( E ), Coq4 ( F ), Coq5 ( G ) and Coq7 ( H ) in MEFs from Coq9 +/+ and Coq9 R239X mice after supplementation with VA at 1 mM ( I-L ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( I), COQ4 ( J ), COQ5 ( K ) and COQ7 ( L ), and the quantitation of the protein bands in the brain of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. ( M-P ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( M), COQ4 ( N ), COQ5 ( O ) and COQ7 ( P ), and the quantitation of the protein bands in the kidney of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. ( Q-T ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( Q), COQ4 ( R ), COQ5 ( S ) and COQ7 ( T ), and the quantitation of the protein bands in the liver of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. (U and V ) Relative mRNA levels of the genes Coq4 ( U ) and Coq5 ( V ) in brain from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM (W and X ) Relative mRNA levels of the genes Coq4 ( W ) and Coq5 ( X ) in the kidneys from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM (Y and Z ) Relative mRNA levels of the genes Coq4 ( Y ) and Coq5 ( Z ) in the liver from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, differences versus Coq9 +/+ ; +P < 0.05, ++P < 0.01, +++P < 0.001, versus Coq9 R239X ; (one-way ANOVA with a Tukey's post hoc test; n = 3 for each group for cell samples, n = 5 for each group for tissue samples).
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
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1) Product Images from "The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency"

Article Title: The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency

Journal: Redox Biology

doi: 10.1016/j.redox.2022.102403

Effect of VA treatment on the levels of CoQ biosynthetic proteins and mRNA levels in the MEFs and tissues of Coq9 R239X mice. ( A-D ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( A ), COQ4 ( B ), COQ5 ( C ) and COQ7 ( D ), and the quantitation of the protein bands in MEFs from Coq9 +/+ and Coq9 R239X mice after supplementation with VA at 1 mM ( E-H ) Relative mRNA levels for the genes Coq2 ( E ), Coq4 ( F ), Coq5 ( G ) and Coq7 ( H ) in MEFs from Coq9 +/+ and Coq9 R239X mice after supplementation with VA at 1 mM ( I-L ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( I), COQ4 ( J ), COQ5 ( K ) and COQ7 ( L ), and the quantitation of the protein bands in the brain of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. ( M-P ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( M), COQ4 ( N ), COQ5 ( O ) and COQ7 ( P ), and the quantitation of the protein bands in the kidney of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. ( Q-T ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( Q), COQ4 ( R ), COQ5 ( S ) and COQ7 ( T ), and the quantitation of the protein bands in the liver of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. (U and V ) Relative mRNA levels of the genes Coq4 ( U ) and Coq5 ( V ) in brain from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM (W and X ) Relative mRNA levels of the genes Coq4 ( W ) and Coq5 ( X ) in the kidneys from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM (Y and Z ) Relative mRNA levels of the genes Coq4 ( Y ) and Coq5 ( Z ) in the liver from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, differences versus Coq9 +/+ ; +P < 0.05, ++P < 0.01, +++P < 0.001, versus Coq9 R239X ; (one-way ANOVA with a Tukey's post hoc test; n = 3 for each group for cell samples, n = 5 for each group for tissue samples).
Figure Legend Snippet: Effect of VA treatment on the levels of CoQ biosynthetic proteins and mRNA levels in the MEFs and tissues of Coq9 R239X mice. ( A-D ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( A ), COQ4 ( B ), COQ5 ( C ) and COQ7 ( D ), and the quantitation of the protein bands in MEFs from Coq9 +/+ and Coq9 R239X mice after supplementation with VA at 1 mM ( E-H ) Relative mRNA levels for the genes Coq2 ( E ), Coq4 ( F ), Coq5 ( G ) and Coq7 ( H ) in MEFs from Coq9 +/+ and Coq9 R239X mice after supplementation with VA at 1 mM ( I-L ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( I), COQ4 ( J ), COQ5 ( K ) and COQ7 ( L ), and the quantitation of the protein bands in the brain of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. ( M-P ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( M), COQ4 ( N ), COQ5 ( O ) and COQ7 ( P ), and the quantitation of the protein bands in the kidney of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. ( Q-T ) Representative images of Western blots of the CoQ biosynthetic proteins COQ2 ( Q), COQ4 ( R ), COQ5 ( S ) and COQ7 ( T ), and the quantitation of the protein bands in the liver of Coq9 +/+ mice, Coq9 +/+ mice after 1% VA treatment, Coq9 R239X mice and Coq9 R239X mice after 1% VA treatment. (U and V ) Relative mRNA levels of the genes Coq4 ( U ) and Coq5 ( V ) in brain from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM (W and X ) Relative mRNA levels of the genes Coq4 ( W ) and Coq5 ( X ) in the kidneys from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM (Y and Z ) Relative mRNA levels of the genes Coq4 ( Y ) and Coq5 ( Z ) in the liver from Coq9 +/+ and Coq9 R239X mice after the supplementation with VA at 1 mM. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, differences versus Coq9 +/+ ; +P < 0.05, ++P < 0.01, +++P < 0.001, versus Coq9 R239X ; (one-way ANOVA with a Tukey's post hoc test; n = 3 for each group for cell samples, n = 5 for each group for tissue samples).

Techniques Used: Western Blot, Quantitation Assay


Structured Review

Proteintech mouse anti coq5
Mouse Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti coq5/product/Proteintech
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mouse anti coq5 - by Bioz Stars, 2024-06
86/100 stars

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Structured Review

Proteintech anti coq5
ADCK4 interacted with <t>COQ5</t> and stabilized complex Q in podocytes. (A) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by nanoflow LC-MS/MS. (B) GO analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. (C) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9 were significantly reduced in ADCK4 KO podocytes. (D) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. (E) Effect of ADCK4 mutations on COQ5 rescue in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild-type ADCK4. (F) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by AA. Unphosphorylated p38 was used as the loading control. Densitometry analyses in (C–E) are representative of at least three independent experiments and band intensities were normalized to that of β-actin. *P<0.05, **P<0.005; t test.
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
86/100 stars

Images

1) Product Images from "ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment"

Article Title: ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment

Journal: Journal of the American Society of Nephrology : JASN

doi: 10.1681/ASN.2019070756

ADCK4 interacted with COQ5 and stabilized complex Q in podocytes. (A) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by nanoflow LC-MS/MS. (B) GO analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. (C) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9 were significantly reduced in ADCK4 KO podocytes. (D) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. (E) Effect of ADCK4 mutations on COQ5 rescue in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild-type ADCK4. (F) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by AA. Unphosphorylated p38 was used as the loading control. Densitometry analyses in (C–E) are representative of at least three independent experiments and band intensities were normalized to that of β-actin. *P<0.05, **P<0.005; t test.
Figure Legend Snippet: ADCK4 interacted with COQ5 and stabilized complex Q in podocytes. (A) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by nanoflow LC-MS/MS. (B) GO analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. (C) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9 were significantly reduced in ADCK4 KO podocytes. (D) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. (E) Effect of ADCK4 mutations on COQ5 rescue in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild-type ADCK4. (F) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by AA. Unphosphorylated p38 was used as the loading control. Densitometry analyses in (C–E) are representative of at least three independent experiments and band intensities were normalized to that of β-actin. *P<0.05, **P<0.005; t test.

Techniques Used: Isolation, Liquid Chromatography with Mass Spectroscopy, Activity Assay, Binding Assay, Western Blot, Expressing, Mutagenesis


Structured Review

Proteintech anti coq5
ADCK4 interacted with <t>COQ5</t> and stabilized complex Q in podocytes. (A) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by nanoflow LC-MS/MS. (B) GO analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. (C) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9 were significantly reduced in ADCK4 KO podocytes. (D) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. (E) Effect of ADCK4 mutations on COQ5 rescue in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild-type ADCK4. (F) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by AA. Unphosphorylated p38 was used as the loading control. Densitometry analyses in (C–E) are representative of at least three independent experiments and band intensities were normalized to that of β-actin. *P<0.05, **P<0.005; t test.
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
91/100 stars

Images

1) Product Images from "ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment"

Article Title: ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment

Journal: Journal of the American Society of Nephrology : JASN

doi: 10.1681/ASN.2019070756

ADCK4 interacted with COQ5 and stabilized complex Q in podocytes. (A) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by nanoflow LC-MS/MS. (B) GO analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. (C) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9 were significantly reduced in ADCK4 KO podocytes. (D) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. (E) Effect of ADCK4 mutations on COQ5 rescue in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild-type ADCK4. (F) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by AA. Unphosphorylated p38 was used as the loading control. Densitometry analyses in (C–E) are representative of at least three independent experiments and band intensities were normalized to that of β-actin. *P<0.05, **P<0.005; t test.
Figure Legend Snippet: ADCK4 interacted with COQ5 and stabilized complex Q in podocytes. (A) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by nanoflow LC-MS/MS. (B) GO analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. (C) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9 were significantly reduced in ADCK4 KO podocytes. (D) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. (E) Effect of ADCK4 mutations on COQ5 rescue in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild-type ADCK4. (F) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by AA. Unphosphorylated p38 was used as the loading control. Densitometry analyses in (C–E) are representative of at least three independent experiments and band intensities were normalized to that of β-actin. *P<0.05, **P<0.005; t test.

Techniques Used: Isolation, Liquid Chromatography with Mass Spectroscopy, Activity Assay, Binding Assay, Western Blot, Expressing, Mutagenesis


Structured Review

Proteintech anti coq5
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
91/100 stars

Images


Structured Review

Proteintech anti coq5
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
91/100 stars

Images


Structured Review

Proteintech anti coq5
( A ) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by NanoLC-MS/MS. ( B ) Gene ontology analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. ( C ) Immunoblot showed that proteins in complex Q, COQ3, <t>COQ5,</t> and COQ9, were significantly reduced in ADCK4 knockout (KO) podocytes. ( D ) Densitometry analysis. Data are representative of at least three independent experiments and band intensities were normalized to that of β-actin. * p < 0.05; t -test. ( E ) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. ( F ) Effect of ADCK4 mutations on COQ5 rescued in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild type ADCK4. ( G ) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by arachidonic acid (AA). Unphosphorylated p38 was used as the loading control.
Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti coq5/product/Proteintech
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti coq5 - by Bioz Stars, 2024-06
91/100 stars

Images

1) Product Images from "ADCK4 deficiency destabilizes the coenzyme Q complex, which is rescued by 2,4-dihydroxybenzoic acid treatment"

Article Title: ADCK4 deficiency destabilizes the coenzyme Q complex, which is rescued by 2,4-dihydroxybenzoic acid treatment

Journal: bioRxiv

doi: 10.1101/712323

( A ) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by NanoLC-MS/MS. ( B ) Gene ontology analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. ( C ) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9, were significantly reduced in ADCK4 knockout (KO) podocytes. ( D ) Densitometry analysis. Data are representative of at least three independent experiments and band intensities were normalized to that of β-actin. * p < 0.05; t -test. ( E ) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. ( F ) Effect of ADCK4 mutations on COQ5 rescued in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild type ADCK4. ( G ) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by arachidonic acid (AA). Unphosphorylated p38 was used as the loading control.
Figure Legend Snippet: ( A ) ADCK4-interacting proteins isolated form HEK293 cells overexpressing ADCK4-3XFLAG. Both cytoplasmic and mitochondrial proteins were detected as interactors by NanoLC-MS/MS. ( B ) Gene ontology analysis showed that ADCK4-interacting mitochondrial proteins are associated with transferase activity, oxidoreductase activity, nucleotide binding, and ATPase activity. ( C ) Immunoblot showed that proteins in complex Q, COQ3, COQ5, and COQ9, were significantly reduced in ADCK4 knockout (KO) podocytes. ( D ) Densitometry analysis. Data are representative of at least three independent experiments and band intensities were normalized to that of β-actin. * p < 0.05; t -test. ( E ) Decreased COQ5 protein level was rescued by heterologous ADCK4 expression and 2,4-diHB treatment in ADCK4 KO podocytes. ( F ) Effect of ADCK4 mutations on COQ5 rescued in ADCK4 KO podocytes. COQ5 was rescued to a lower extent by truncated ADCK4 mutant proteins, whereas ADCK4 mutant proteins harboring missense variations were not different from wild type ADCK4. ( G ) Immunoblot analysis of the MAPK pathway. p-p38 and p-pERK were increased in ADCK4 KO podocytes and p-p38 was further induced by arachidonic acid (AA). Unphosphorylated p38 was used as the loading control.

Techniques Used: Isolation, Tandem Mass Spectroscopy, Activity Assay, Binding Assay, Western Blot, Knock-Out, Expressing, Mutagenesis

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    Proteintech anti coq5
    Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti coq5/product/Proteintech
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    Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coq5/product/Proteintech
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    coq5 - by Bioz Stars, 2024-06
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    Proteintech mouse anti coq5
    Mouse Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti coq5/product/Proteintech
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