anti connexin 43 antibody produced in rabbit  (Alomone Labs)


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    Alomone Labs anti connexin 43 antibody produced in rabbit
    Anti Connexin 43 Antibody Produced In Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti connexin 43 antibody produced in rabbit/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
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    anti connexin 43 antibody produced in rabbit - by Bioz Stars, 2022-09
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    Alomone Labs cx43 from alomone
    <t>Cx43</t> is primarily localized to astrocytes and vascular cells. (a) Around an artery, majority of the large puncta was not directly associated with vascular cells. (b) A vertical view from the highlighted area in (a) was obtained by 90 0 rotation of the z-stack. On the right, fluorescence intensity profile for the three labeling from (b). (c–d) In the relay, large puncta were also not directly located on the blood vessels contrarily to the string-like structures which were enclosed by the mural cells (d panel and the intensity profile). (e–f) Around the finest capillaries, Cx43-puncta were mostly outside of the blood vessels. (h) In veins, putative labeling was not directly associated with mural cells. (i–j) Bright Cx43 puncta around blood vessels were localized to astroglia labeled for glial fibrillary acidic protein (GFAP). (k–m) Cx43-positive strings were associated with endothelial cells labeled for platelet endothelial cell adhesion molecule-1 (PECAM-1). Scale bar 5 μm.
    Cx43 From Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx43 from alomone/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cx43 from alomone - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    95
    Alomone Labs anti connexin 43 antibody produced in rabbit
    <t>Cx43</t> is primarily localized to astrocytes and vascular cells. (a) Around an artery, majority of the large puncta was not directly associated with vascular cells. (b) A vertical view from the highlighted area in (a) was obtained by 90 0 rotation of the z-stack. On the right, fluorescence intensity profile for the three labeling from (b). (c–d) In the relay, large puncta were also not directly located on the blood vessels contrarily to the string-like structures which were enclosed by the mural cells (d panel and the intensity profile). (e–f) Around the finest capillaries, Cx43-puncta were mostly outside of the blood vessels. (h) In veins, putative labeling was not directly associated with mural cells. (i–j) Bright Cx43 puncta around blood vessels were localized to astroglia labeled for glial fibrillary acidic protein (GFAP). (k–m) Cx43-positive strings were associated with endothelial cells labeled for platelet endothelial cell adhesion molecule-1 (PECAM-1). Scale bar 5 μm.
    Anti Connexin 43 Antibody Produced In Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti connexin 43 antibody produced in rabbit/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti connexin 43 antibody produced in rabbit - by Bioz Stars, 2022-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Cx43 is primarily localized to astrocytes and vascular cells. (a) Around an artery, majority of the large puncta was not directly associated with vascular cells. (b) A vertical view from the highlighted area in (a) was obtained by 90 0 rotation of the z-stack. On the right, fluorescence intensity profile for the three labeling from (b). (c–d) In the relay, large puncta were also not directly located on the blood vessels contrarily to the string-like structures which were enclosed by the mural cells (d panel and the intensity profile). (e–f) Around the finest capillaries, Cx43-puncta were mostly outside of the blood vessels. (h) In veins, putative labeling was not directly associated with mural cells. (i–j) Bright Cx43 puncta around blood vessels were localized to astroglia labeled for glial fibrillary acidic protein (GFAP). (k–m) Cx43-positive strings were associated with endothelial cells labeled for platelet endothelial cell adhesion molecule-1 (PECAM-1). Scale bar 5 μm.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: Cx43 is primarily localized to astrocytes and vascular cells. (a) Around an artery, majority of the large puncta was not directly associated with vascular cells. (b) A vertical view from the highlighted area in (a) was obtained by 90 0 rotation of the z-stack. On the right, fluorescence intensity profile for the three labeling from (b). (c–d) In the relay, large puncta were also not directly located on the blood vessels contrarily to the string-like structures which were enclosed by the mural cells (d panel and the intensity profile). (e–f) Around the finest capillaries, Cx43-puncta were mostly outside of the blood vessels. (h) In veins, putative labeling was not directly associated with mural cells. (i–j) Bright Cx43 puncta around blood vessels were localized to astroglia labeled for glial fibrillary acidic protein (GFAP). (k–m) Cx43-positive strings were associated with endothelial cells labeled for platelet endothelial cell adhesion molecule-1 (PECAM-1). Scale bar 5 μm.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: Fluorescence, Labeling

    Hierarchy within the vascular tree dictates distinct patterns of Cx43 clustering. (a) Cx43 is highly concentrated along retinal vasculature. Inset shows the arteries from the same area labeled for smooth muscle actin (SMA). Scale bar 200 μm. (b) In the superficial layer from area b in (a), majority of puncta were associated with vasculature: arteries, veins and capillaries and lower number of similar size puncta were present outside of the blood vessels. Scale bar 50 μm. (c) In the branches of major arteries from an area similar to the area c in (a), string-like structures running along the blood vessels were found in addition to the puncta. Scale bar 50 μm. (d) Density of the Cx43 puncta was the highest on veins and capillaries. The strings were found predominantly in relay regions connecting artery with capillaries. Density of Cx43 puncta outside of the blood vessels was significantly lower and no strings were detected. (e) Dendrogram created by cluster analysis of data from (d). (f) Schematic presentation of Cx43 expression pattern in retinal vasculature. (g) In a vertical section through the retina (created by rotation of a thin slit from the z-stack), the string-like structures from the superficial layer were extending along some capillaries into the intermediate and deep vascular layers. Scale bar 50 μm. (h) Distribution of Cx43 shows that Cx43 (puncta and strings combined) is predominantly expressed on the vasculature throughout the retina. The relays have the highest densities of Cx43-positive structures. The intermediate and deep layer capillaries above these relay zones also have the highest expression of Cx43 in string-like structures. The non-vascular expression in the superficial layer reflects the puncta outside of the blood vessels; no puncta of the same size and labeling intensity was detected in the intermediate and deep layers. Data are shown as average ± SD; arteries, vein and transitions were quantified in 6 samples from 6 mice; capillaries and non-vascular areas in n = 12 samples from n = 6 mice. ANOVA on ranks, * p = 0.0001.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: Hierarchy within the vascular tree dictates distinct patterns of Cx43 clustering. (a) Cx43 is highly concentrated along retinal vasculature. Inset shows the arteries from the same area labeled for smooth muscle actin (SMA). Scale bar 200 μm. (b) In the superficial layer from area b in (a), majority of puncta were associated with vasculature: arteries, veins and capillaries and lower number of similar size puncta were present outside of the blood vessels. Scale bar 50 μm. (c) In the branches of major arteries from an area similar to the area c in (a), string-like structures running along the blood vessels were found in addition to the puncta. Scale bar 50 μm. (d) Density of the Cx43 puncta was the highest on veins and capillaries. The strings were found predominantly in relay regions connecting artery with capillaries. Density of Cx43 puncta outside of the blood vessels was significantly lower and no strings were detected. (e) Dendrogram created by cluster analysis of data from (d). (f) Schematic presentation of Cx43 expression pattern in retinal vasculature. (g) In a vertical section through the retina (created by rotation of a thin slit from the z-stack), the string-like structures from the superficial layer were extending along some capillaries into the intermediate and deep vascular layers. Scale bar 50 μm. (h) Distribution of Cx43 shows that Cx43 (puncta and strings combined) is predominantly expressed on the vasculature throughout the retina. The relays have the highest densities of Cx43-positive structures. The intermediate and deep layer capillaries above these relay zones also have the highest expression of Cx43 in string-like structures. The non-vascular expression in the superficial layer reflects the puncta outside of the blood vessels; no puncta of the same size and labeling intensity was detected in the intermediate and deep layers. Data are shown as average ± SD; arteries, vein and transitions were quantified in 6 samples from 6 mice; capillaries and non-vascular areas in n = 12 samples from n = 6 mice. ANOVA on ranks, * p = 0.0001.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: Labeling, Expressing, Mouse Assay

    Validation and optimization of Connexin antibodies used in this study. (a–d) Cx37 labeling revealed just a few puncta (green) on blood vessels (blue) in pure PFA fixative (a) and entire outlines of contractile cells in carbodiimide-based fixatives (b and c). Ganglion cells were more visible in PFA fixative (asterisks); the lowest background was in carbodiimide. Pre-absorption of the antibody with a control peptide eliminated the labeling (d). (e–h) Cx40 (green) was associated with outlines of endothelial cells (blue) in all fixative with the less background in carbodiimide (f). Incubation of primary antibody eliminated the labeling (h) with the exception of labeled microglia (arrow). (i–k) Cx43s-positive puncta (green, Sigma antibody) were associated with vasculature in PFA fixative (i); in addition string-like structures along endothelial cell contacts (blue) became visible in carbodiimide fixative (j–k). (l) Omission of the primary antibody eliminated punctate and string-like structures; the microglia was labeled by the secondary antibody (arrow). (m–p) Cx43a produced identical pattern as Cx43s in carbodiimide containing fixatives (n, o). In pure PFA fixative, this antibody labeled well punctate and string-like structures; however the background was significantly higher (m). Incubation of the primary antibodies with control peptide eliminated the specific labeling (p). (q–s) Morphology of DsRed expressing pericytes was best preserved in PFA-containing fixatives (q and s); the DsRed labeling in pericyte processes was lost in pure carbodiimide fixative (q). (t) Summary of fixation procedure. Red crosses indicate signal, black crosses – background; the number of crosses indicates intensity. Scale bar 10 μm. Cx43s – Cx43 antibody from Sigma, Cx43a – Cx43 antibody from Alomone, NB – Neurobiotin.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: Validation and optimization of Connexin antibodies used in this study. (a–d) Cx37 labeling revealed just a few puncta (green) on blood vessels (blue) in pure PFA fixative (a) and entire outlines of contractile cells in carbodiimide-based fixatives (b and c). Ganglion cells were more visible in PFA fixative (asterisks); the lowest background was in carbodiimide. Pre-absorption of the antibody with a control peptide eliminated the labeling (d). (e–h) Cx40 (green) was associated with outlines of endothelial cells (blue) in all fixative with the less background in carbodiimide (f). Incubation of primary antibody eliminated the labeling (h) with the exception of labeled microglia (arrow). (i–k) Cx43s-positive puncta (green, Sigma antibody) were associated with vasculature in PFA fixative (i); in addition string-like structures along endothelial cell contacts (blue) became visible in carbodiimide fixative (j–k). (l) Omission of the primary antibody eliminated punctate and string-like structures; the microglia was labeled by the secondary antibody (arrow). (m–p) Cx43a produced identical pattern as Cx43s in carbodiimide containing fixatives (n, o). In pure PFA fixative, this antibody labeled well punctate and string-like structures; however the background was significantly higher (m). Incubation of the primary antibodies with control peptide eliminated the specific labeling (p). (q–s) Morphology of DsRed expressing pericytes was best preserved in PFA-containing fixatives (q and s); the DsRed labeling in pericyte processes was lost in pure carbodiimide fixative (q). (t) Summary of fixation procedure. Red crosses indicate signal, black crosses – background; the number of crosses indicates intensity. Scale bar 10 μm. Cx43s – Cx43 antibody from Sigma, Cx43a – Cx43 antibody from Alomone, NB – Neurobiotin.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: Labeling, Incubation, Produced, Expressing

    Cx37, Cx40, and Cx43 are expressed in retinal neurons at much lower levels than in the vasculature. (a) Cx37 expression shown in a vertical view, created by rotation of a z-stack from the corresponding retinal whole mount region in (b). (b) Confocal projection of a retinal whole mount at the GCL. Some ganglion cells expressed Cx37 at their cell membrane (asterisks, insert). (c) Blood vessels had higher expression of Cx37 (blue) than ganglion cells (red). (d–e) Cx40 was weakly expressed in numerous nuclei of inner retinal neurons. High expression of Cx40 was detected in blood vessels (f, blue). (g–i) Cx43 was expressed in nuclei (insert) and processes of inner retinal neurons (g, four bright bands in the inner plexiform layer). The expression in neurons was lower than in blood vessels (i). Scale bar 20 μm. ONL – outer nuclear layer, OPL – outer plexiform layer, INL – inner nuclear layer, IPL – inner plexiform layer, GCL – ganglion cell layer.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: Cx37, Cx40, and Cx43 are expressed in retinal neurons at much lower levels than in the vasculature. (a) Cx37 expression shown in a vertical view, created by rotation of a z-stack from the corresponding retinal whole mount region in (b). (b) Confocal projection of a retinal whole mount at the GCL. Some ganglion cells expressed Cx37 at their cell membrane (asterisks, insert). (c) Blood vessels had higher expression of Cx37 (blue) than ganglion cells (red). (d–e) Cx40 was weakly expressed in numerous nuclei of inner retinal neurons. High expression of Cx40 was detected in blood vessels (f, blue). (g–i) Cx43 was expressed in nuclei (insert) and processes of inner retinal neurons (g, four bright bands in the inner plexiform layer). The expression in neurons was lower than in blood vessels (i). Scale bar 20 μm. ONL – outer nuclear layer, OPL – outer plexiform layer, INL – inner nuclear layer, IPL – inner plexiform layer, GCL – ganglion cell layer.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: Expressing

    Cx37 is expressed in all vascular cells. (a) In confocal section through an artery, puncta of cx37 (green) were present on endothelial cells (blue). (b) In the same artery, Cx37 was also co-localized on mural cells (magenta). (c) In the cross section of the artery from (a-b) Cx37 was present on both endothelial and contractile cells as reflected by the intensity profile on the right. (d–e) Cx37 was present on the entire membrane of the mural cells. (f–i) Similar to artery, in a capillary Cx43 was expressed in both endothelial and contractile cells. (j–I) In veins the strongest Cx37 labeling was around pericytes. Cx37 puncta were present on endothelial cells. Scale bar 5 μm.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: Cx37 is expressed in all vascular cells. (a) In confocal section through an artery, puncta of cx37 (green) were present on endothelial cells (blue). (b) In the same artery, Cx37 was also co-localized on mural cells (magenta). (c) In the cross section of the artery from (a-b) Cx37 was present on both endothelial and contractile cells as reflected by the intensity profile on the right. (d–e) Cx37 was present on the entire membrane of the mural cells. (f–i) Similar to artery, in a capillary Cx43 was expressed in both endothelial and contractile cells. (j–I) In veins the strongest Cx37 labeling was around pericytes. Cx37 puncta were present on endothelial cells. Scale bar 5 μm.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: Labeling

    Cx43 promotes vascular coupling between the capillaries and the artery. (a-c) The relay zones were identified based on the staining against smooth muscle actin (SMA). The string-like Cx43-positive structures started at the primary artery and continued uninterrupted beyond SMA-labeling into selected capillaries (arrows). Some capillaries had much weaker or no string-like expression (arrowheads). The strings from the superficial layer in (a) extended into intermediate (b) and deep (c) vascular layer capillaries using the shortest route from the artery. Scale bar 50 μm. (d) In the projection of all layers, string-bearing selected capillaries created a specialized vascular domain – vascular relay - spanning all three layers. The bottom panel shows a vertical view. Scale bar 50 μm. (e) Neurobiotin injected into a pericyte (arrow) in relay area spread throughout the large area. (f) Neurobiotin injected into the area outside the relay was restricted to a few pericytes. (g) Quantification shows that under normal conditions cells are extensively coupled through GJs in vascular relay (data are shown as average ± SD; standard: 3.3 ± 2.1, 15 samples, 8 mice; sensor: 23.7 ± 9.6, 11 samples, 6 mice; T-test, p = 0.00003). (h) Distribution of Cx43-positive strings across vascular layers shows that in the vascular relay region Neurobiotin spread farther into intermediate and deep vascular layers.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: Cx43 promotes vascular coupling between the capillaries and the artery. (a-c) The relay zones were identified based on the staining against smooth muscle actin (SMA). The string-like Cx43-positive structures started at the primary artery and continued uninterrupted beyond SMA-labeling into selected capillaries (arrows). Some capillaries had much weaker or no string-like expression (arrowheads). The strings from the superficial layer in (a) extended into intermediate (b) and deep (c) vascular layer capillaries using the shortest route from the artery. Scale bar 50 μm. (d) In the projection of all layers, string-bearing selected capillaries created a specialized vascular domain – vascular relay - spanning all three layers. The bottom panel shows a vertical view. Scale bar 50 μm. (e) Neurobiotin injected into a pericyte (arrow) in relay area spread throughout the large area. (f) Neurobiotin injected into the area outside the relay was restricted to a few pericytes. (g) Quantification shows that under normal conditions cells are extensively coupled through GJs in vascular relay (data are shown as average ± SD; standard: 3.3 ± 2.1, 15 samples, 8 mice; sensor: 23.7 ± 9.6, 11 samples, 6 mice; T-test, p = 0.00003). (h) Distribution of Cx43-positive strings across vascular layers shows that in the vascular relay region Neurobiotin spread farther into intermediate and deep vascular layers.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: Staining, Labeling, Expressing, Injection, Mouse Assay

    In the arteries and relays gap junctions of endothelial cells strictly colocalize with tight junctions. (a–d) In the artery, Cx40 (green) was strictly colocalized with tight junctions (magenta, arrows) and less precisely with adherens proteins (blue). In a merged image of claudin5 and PECAM (d), the colocalization was not precise. White line highlights the region used for fluorescent intensity profile. Note that claudin5 and Cx40 profiles closely match and differ from the PECAM profile. (e–h) In the vascular relay, Cx43-positive strings were colocalized with tight junctions including strings and small varicosities (arrows). (i–l) In the capillary, Cx43 was not detected along tight junctions. Scale bar 10 μm.

    Journal: The Journal of comparative neurology

    Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

    doi: 10.1002/cne.24699

    Figure Lengend Snippet: In the arteries and relays gap junctions of endothelial cells strictly colocalize with tight junctions. (a–d) In the artery, Cx40 (green) was strictly colocalized with tight junctions (magenta, arrows) and less precisely with adherens proteins (blue). In a merged image of claudin5 and PECAM (d), the colocalization was not precise. White line highlights the region used for fluorescent intensity profile. Note that claudin5 and Cx40 profiles closely match and differ from the PECAM profile. (e–h) In the vascular relay, Cx43-positive strings were colocalized with tight junctions including strings and small varicosities (arrows). (i–l) In the capillary, Cx43 was not detected along tight junctions. Scale bar 10 μm.

    Article Snippet: The widely used antibody to Cx43 from Sigma (Cx43s, Cat # C6219, – ) did not have a control peptide and was validated by comparison with a different, less common, antibody to Cx43 from Alomone (Cx43a, Cat # ACC-201, – ).

    Techniques: