Structured Review

Servicebio Inc anti cleaved caspase 3
Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo.  a  Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times.  d  H  E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P
Anti Cleaved Caspase 3, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cleaved caspase 3/product/Servicebio Inc
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
anti cleaved caspase 3 - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin"

Article Title: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin

Journal: Clinical Epigenetics

doi: 10.1186/s13148-018-0605-x

Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo.  a  Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times.  d  H  E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P
Figure Legend Snippet: Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo. a Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times. d H E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P

Techniques Used: In Vivo, Mouse Assay, TUNEL Assay, Staining

2) Product Images from "Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin"

Article Title: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin

Journal: Clinical Epigenetics

doi: 10.1186/s13148-018-0605-x

Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo.  a  Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times.  d  H  E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P
Figure Legend Snippet: Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo. a Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times. d H E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P

Techniques Used: In Vivo, Mouse Assay, TUNEL Assay, Staining

3) Product Images from "Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity"

Article Title: Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity

Journal: Mediators of Inflammation

doi: 10.1155/2018/4608017

Dunye Guanxinning inhibited myocardial apoptosis. (a) Immunochemical TUNEL staining; (b) multiples of optical density of TUNEL staining from Figure 2(d) ( n = 3); (c) immunochemical staining of anti-caspase-3 (cleaved) antibody; (d) multiples of cleaved caspase-3 staining positive cells from Figure 2(a) (each n = 3); (e) caspase-3 immunoblot of left ventricular tissue (each n = 4). Data are expressed as mean ± SD. ∗ P
Figure Legend Snippet: Dunye Guanxinning inhibited myocardial apoptosis. (a) Immunochemical TUNEL staining; (b) multiples of optical density of TUNEL staining from Figure 2(d) ( n = 3); (c) immunochemical staining of anti-caspase-3 (cleaved) antibody; (d) multiples of cleaved caspase-3 staining positive cells from Figure 2(a) (each n = 3); (e) caspase-3 immunoblot of left ventricular tissue (each n = 4). Data are expressed as mean ± SD. ∗ P

Techniques Used: TUNEL Assay, Staining

4) Product Images from "Histone methyltransferase NSD2 regulates apoptosis and chemosensitivity in osteosarcoma"

Article Title: Histone methyltransferase NSD2 regulates apoptosis and chemosensitivity in osteosarcoma

Journal: Cell Death & Disease

doi: 10.1038/s41419-019-1347-1

NSD2 knockdown inhibits osteosarcoma genesis in vivo. a Macroscopic image of tumour size. Diagrams of tumour volume ( b ) and tumour weight ( c ) for mice sacrificed at the indicated time points are shown. d Haematoxylin–eosin staining and transferase-mediated deoxyuridine triphosphate-biotin nick end labelling, cleaved caspase 3 and Ki67 staining of representative tumours in the control group and two NSD2-KD groups. ** P
Figure Legend Snippet: NSD2 knockdown inhibits osteosarcoma genesis in vivo. a Macroscopic image of tumour size. Diagrams of tumour volume ( b ) and tumour weight ( c ) for mice sacrificed at the indicated time points are shown. d Haematoxylin–eosin staining and transferase-mediated deoxyuridine triphosphate-biotin nick end labelling, cleaved caspase 3 and Ki67 staining of representative tumours in the control group and two NSD2-KD groups. ** P

Techniques Used: In Vivo, Mouse Assay, Staining

Related Articles

Staining:

Article Title: Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity
Article Snippet: .. Formalin-fixed and paraffin-embedded tissue section blocks were cut in 6 μ m sections, followed by immunohistochemical staining with primary antibodies of anti-cleaved caspase-3 (GB13009 Whan Servicebio Technology Co. Ltd., Wuhan, China), anti-CD68 (GB13067-1, Servicebio), anti-MPO (GB13224, Servicebio), or anti-CD15 (GTX37536, GeneTex, Irvine, USA) and goat anti-mouse or anti-rabbit IgG secondary antibodies. .. The EnVision (Peroxidase/DAB, Rabbit/Mouse) detection system was used to evaluate the immunohistochemical staining.

Article Title: Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity
Article Snippet: .. Immunohistochemical Staining Formalin-fixed and paraffin-embedded tissue section blocks were cut in 6 μ m sections, followed by immunohistochemical staining with primary antibodies of anti-cleaved caspase-3 (GB13009 Whan Servicebio Technology Co. Ltd., Wuhan, China), anti-CD68 (GB13067-1, Servicebio), anti-MPO (GB13224, Servicebio), or anti-CD15 (GTX37536, GeneTex, Irvine, USA) and goat anti-mouse or anti-rabbit IgG secondary antibodies. .. The EnVision (Peroxidase/DAB, Rabbit/Mouse) detection system was used to evaluate the immunohistochemical staining.

Immunohistochemistry:

Article Title: Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity
Article Snippet: .. Formalin-fixed and paraffin-embedded tissue section blocks were cut in 6 μ m sections, followed by immunohistochemical staining with primary antibodies of anti-cleaved caspase-3 (GB13009 Whan Servicebio Technology Co. Ltd., Wuhan, China), anti-CD68 (GB13067-1, Servicebio), anti-MPO (GB13224, Servicebio), or anti-CD15 (GTX37536, GeneTex, Irvine, USA) and goat anti-mouse or anti-rabbit IgG secondary antibodies. .. The EnVision (Peroxidase/DAB, Rabbit/Mouse) detection system was used to evaluate the immunohistochemical staining.

Article Title: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin
Article Snippet: .. Immunohistochemistry After deparaffinization, rehydration, antigen retrieval, and blockade of endogenous peroxidase, tissues were incubated with anti-TUNEL (Roche), anti-cleaved Caspase 3 (Servicebio), anti-Ki67 (Servicebio), and anti-CD31 (Abways) primary antibodies at 4 °C overnight. .. Next, secondary antibodies were applied, and diaminobenzidine (DAB) (Dako) solution was used as a chromogen.

Article Title: Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity
Article Snippet: .. Immunohistochemical Staining Formalin-fixed and paraffin-embedded tissue section blocks were cut in 6 μ m sections, followed by immunohistochemical staining with primary antibodies of anti-cleaved caspase-3 (GB13009 Whan Servicebio Technology Co. Ltd., Wuhan, China), anti-CD68 (GB13067-1, Servicebio), anti-MPO (GB13224, Servicebio), or anti-CD15 (GTX37536, GeneTex, Irvine, USA) and goat anti-mouse or anti-rabbit IgG secondary antibodies. .. The EnVision (Peroxidase/DAB, Rabbit/Mouse) detection system was used to evaluate the immunohistochemical staining.

Inhibition:

Article Title: Histone methyltransferase NSD2 regulates apoptosis and chemosensitivity in osteosarcoma
Article Snippet: .. After deparaffinization, rehydration, antigen retrieval and endogenous peroxidase inhibition, the samples were incubated with anti-NSD2 (Abcam), anti-TUNEL (Roche) and anti-cleaved caspase 3 (Servicebio) antibodies at 4 °C for 8–10 h. After the secondary antibody was applied, diaminobenzidine (DAKO) solution was used as a chromogen. .. Furthermore, haematoxylin (Sigma) staining was performed to identify nuclei.

Incubation:

Article Title: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin
Article Snippet: .. Immunohistochemistry After deparaffinization, rehydration, antigen retrieval, and blockade of endogenous peroxidase, tissues were incubated with anti-TUNEL (Roche), anti-cleaved Caspase 3 (Servicebio), anti-Ki67 (Servicebio), and anti-CD31 (Abways) primary antibodies at 4 °C overnight. .. Next, secondary antibodies were applied, and diaminobenzidine (DAB) (Dako) solution was used as a chromogen.

Article Title: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin
Article Snippet: .. After deparaffinization, rehydration, antigen retrieval, and blockade of endogenous peroxidase, tissues were incubated with anti-TUNEL (Roche), anti-cleaved Caspase 3 (Servicebio), anti-Ki67 (Servicebio), and anti-CD31 (Abways) primary antibodies at 4 °C overnight. .. Next, secondary antibodies were applied, and diaminobenzidine (DAB) (Dako) solution was used as a chromogen.

Article Title: Histone methyltransferase NSD2 regulates apoptosis and chemosensitivity in osteosarcoma
Article Snippet: .. After deparaffinization, rehydration, antigen retrieval and endogenous peroxidase inhibition, the samples were incubated with anti-NSD2 (Abcam), anti-TUNEL (Roche) and anti-cleaved caspase 3 (Servicebio) antibodies at 4 °C for 8–10 h. After the secondary antibody was applied, diaminobenzidine (DAKO) solution was used as a chromogen. .. Furthermore, haematoxylin (Sigma) staining was performed to identify nuclei.

Article Title: Autophagy precedes apoptosis during degeneration of the Kölliker’s organ in the development of rat cochlea
Article Snippet: .. The following primary antibodies were used: anti-MAP LC3II antibody (Santa Cruz Biotecnology, Dallas, TX, USA; sc- 271625; 1:300), anti-SQSTM1/P62 antibody (Abcam, Cambridge, UK; ab109012; 1:200), anti-Beclin1 antibody (Abcam; ab62557; 1:200), anti-Bcl-2 (Servicebio Inc., Woburn, MA, USA; GB12008; 1:200), or anti-cleaved caspase-3 (Servicebio Inc.; GB11009; 1:500) at 4°C overnight, washed three times with PBS for 10 min, and then incubated with a HRP-secondary antibody (goat anti-rabbit and goat anti-mouse 1 : 300) at room temperature for 40 min. Fresh DAB chromogenic reagent was added and incubated for 10 min. .. Isolation of the Kölliker’s organ Rats were anesthetized by rapid induction of hypothermia via immersion in ice for 4-5 min until loss of consciousness, quickly decapitated and had their temporal bones removed.

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    Servicebio Inc anti mouse caspase 3 pab
    Pathological changes of VEEV-RABV-G in adult mice. Groups of 6-week old female ICR mice (n = 5 / group) were i.c. infected with 5 × 10 3  FFU of VEEV-RABV-G, LBNSE, LBNAR or mock-infected with 25 μl DMEM. Brain sagittal sections of infected mice were cut and immunohistochemically (IHC) stained with CD45 (a) or caspase 3 antibody (c). Representative histological images at 200 × magnifications (scale bars, 200 μm) in different brain regions including pons (Pons), thalamus (Thala), hippocampus (Hippo) and cerebral cortex (Cere). Positive DAB signals (brown) representing CD45 (b) or caspase 3 (d) were analyzed by integrated optical density (IOD) of positive signals (brown, DAB) using NIH Image J software (IHC toolbox). Statistical data are presented as means ± SEM. Asterisks indicate significant differences between the indicated experimental groups, * p
    Anti Mouse Caspase 3 Pab, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse caspase 3 pab/product/Servicebio Inc
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse caspase 3 pab - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    92
    Servicebio Inc anti caspase 3
    Immunohistochemical analysis of Ki‐67 and <t>caspase‐3</t> in tumor xenograft tissues after the administration of different treatments ( n = 6 mice/group). Groups: control; Ico, icotinib; Ico‐Pem, sequential Ico followed by Pem; Ico + Pem, concurrent Ico and Pem; Pem, pemetrexed; Pem‐Ico, sequential Pem followed by Ico. Representative immunohistochemical staining results of ( a ) Ki‐67 (stained in brown) and ( b ) caspase‐3 (stained in brown). ( c ) Proliferation rate assessed by Ki‐67 score. ( d ) Apoptosis rate assessed by caspase‐3 score. Ki‐67 score and caspase‐3 score shown as a histogram: columns, mean ± standard deviation. P
    Anti Caspase 3, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 3/product/Servicebio Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Servicebio Inc anti active caspase 3
    F-AgÅPs induce osteosarcoma cell death through the promotion of apoptosis. ( A-D ) Representative images of TUNEL staining and quantification of the apoptotic cell numbers in tumor sections from subcutaneous 143B ( A and B ) or orthotopic SJSA-1 ( C and D ) xenografts-bearing mice treated with solvent, F-AgÅPs or cisplatin for 21 days. Scale bar: 50 µm. n = 3 per group. ( E and F ) Representative active <t>caspase-3</t> staining images ( E ) and quantification of the mean staining intensity ( F ) in tumor sections from mice in ( A and B ). Scale bar: 50 µm. n = 3 per group. ( G ) Flow cytometric analysis of Annexin V-FITC/PI-stained 143B and SJSA-1 receiving different treatments for 24 h. The cells in Q1 to Q4 indicate dead/necrotic cells, late apoptotic/dead cells, early apoptotic cells and live cells, respectively. ( H and I ) Representative TUNEL staining images of 143B and SJSA-1 receiving different treatments for 24 h ( H ) and quantification of the apoptotic cell numbers ( I ). Scale bar: 50 µm. n = 3 per group. ( J ) CCK-8 analysis of the viability of 143B and SJSA-1 treated with solvent, F-AgÅPs or F-AgÅPs + Z-VAD-FMK for 24 h. n = 5 per group. ( K and L ) Representative images of calcein-AM/PI staining ( K ) and quantification of the percentages of live cells ( L ). Scale bar: 100 µm. n = 3 per group. ( M ) Flow cytometric analysis of Annexin V-FITC/PI-stained 143B and SJSA-1 receiving different treatments for 24 h. Data are shown as mean ± SD. * P
    Anti Active Caspase 3, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 92/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti active caspase 3/product/Servicebio Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti active caspase 3 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Servicebio Inc anti cleaved caspase 3
    Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo.  a  Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times.  d  H  E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P
    Anti Cleaved Caspase 3, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3/product/Servicebio Inc
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Pathological changes of VEEV-RABV-G in adult mice. Groups of 6-week old female ICR mice (n = 5 / group) were i.c. infected with 5 × 10 3  FFU of VEEV-RABV-G, LBNSE, LBNAR or mock-infected with 25 μl DMEM. Brain sagittal sections of infected mice were cut and immunohistochemically (IHC) stained with CD45 (a) or caspase 3 antibody (c). Representative histological images at 200 × magnifications (scale bars, 200 μm) in different brain regions including pons (Pons), thalamus (Thala), hippocampus (Hippo) and cerebral cortex (Cere). Positive DAB signals (brown) representing CD45 (b) or caspase 3 (d) were analyzed by integrated optical density (IOD) of positive signals (brown, DAB) using NIH Image J software (IHC toolbox). Statistical data are presented as means ± SEM. Asterisks indicate significant differences between the indicated experimental groups, * p

    Journal: EBioMedicine

    Article Title: A novel rabies vaccine based on infectious propagating particles derived from hybrid VEEV-Rabies replicon

    doi: 10.1016/j.ebiom.2020.102819

    Figure Lengend Snippet: Pathological changes of VEEV-RABV-G in adult mice. Groups of 6-week old female ICR mice (n = 5 / group) were i.c. infected with 5 × 10 3 FFU of VEEV-RABV-G, LBNSE, LBNAR or mock-infected with 25 μl DMEM. Brain sagittal sections of infected mice were cut and immunohistochemically (IHC) stained with CD45 (a) or caspase 3 antibody (c). Representative histological images at 200 × magnifications (scale bars, 200 μm) in different brain regions including pons (Pons), thalamus (Thala), hippocampus (Hippo) and cerebral cortex (Cere). Positive DAB signals (brown) representing CD45 (b) or caspase 3 (d) were analyzed by integrated optical density (IOD) of positive signals (brown, DAB) using NIH Image J software (IHC toolbox). Statistical data are presented as means ± SEM. Asterisks indicate significant differences between the indicated experimental groups, * p

    Article Snippet: For IHC, anti-mouse CD45 mAb (cat. no. GB11066) and anti-mouse caspase-3 pAb (cat. no. GB11009) were purchased from Servicebio technology Co., Ltd. (Wuhan, CHN) FITC or HRP conjugated goat anti-mouse/rabbit IgG were purchased from Proteintech Group Inc. (Chicago, IL, USA).

    Techniques: Mouse Assay, Infection, Immunohistochemistry, Staining, Software

    Immunohistochemical analysis of Ki‐67 and caspase‐3 in tumor xenograft tissues after the administration of different treatments ( n = 6 mice/group). Groups: control; Ico, icotinib; Ico‐Pem, sequential Ico followed by Pem; Ico + Pem, concurrent Ico and Pem; Pem, pemetrexed; Pem‐Ico, sequential Pem followed by Ico. Representative immunohistochemical staining results of ( a ) Ki‐67 (stained in brown) and ( b ) caspase‐3 (stained in brown). ( c ) Proliferation rate assessed by Ki‐67 score. ( d ) Apoptosis rate assessed by caspase‐3 score. Ki‐67 score and caspase‐3 score shown as a histogram: columns, mean ± standard deviation. P

    Journal: Thoracic Cancer

    Article Title: Efficacy of combined icotinib and pemetrexed in EGFR mutant lung adenocarcinoma cell line xenografts

    doi: 10.1111/1759-7714.12818

    Figure Lengend Snippet: Immunohistochemical analysis of Ki‐67 and caspase‐3 in tumor xenograft tissues after the administration of different treatments ( n = 6 mice/group). Groups: control; Ico, icotinib; Ico‐Pem, sequential Ico followed by Pem; Ico + Pem, concurrent Ico and Pem; Pem, pemetrexed; Pem‐Ico, sequential Pem followed by Ico. Representative immunohistochemical staining results of ( a ) Ki‐67 (stained in brown) and ( b ) caspase‐3 (stained in brown). ( c ) Proliferation rate assessed by Ki‐67 score. ( d ) Apoptosis rate assessed by caspase‐3 score. Ki‐67 score and caspase‐3 score shown as a histogram: columns, mean ± standard deviation. P

    Article Snippet: The tissue sections were immunostained by anti‐CD34, anti‐Ki‐67, and anti‐caspase‐3 according to the manufacturer's instructions (Servicebio, Wuhan, China).

    Techniques: Immunohistochemistry, Mouse Assay, End-sequence Profiling, Staining, Standard Deviation

    F-AgÅPs induce osteosarcoma cell death through the promotion of apoptosis. ( A-D ) Representative images of TUNEL staining and quantification of the apoptotic cell numbers in tumor sections from subcutaneous 143B ( A and B ) or orthotopic SJSA-1 ( C and D ) xenografts-bearing mice treated with solvent, F-AgÅPs or cisplatin for 21 days. Scale bar: 50 µm. n = 3 per group. ( E and F ) Representative active caspase-3 staining images ( E ) and quantification of the mean staining intensity ( F ) in tumor sections from mice in ( A and B ). Scale bar: 50 µm. n = 3 per group. ( G ) Flow cytometric analysis of Annexin V-FITC/PI-stained 143B and SJSA-1 receiving different treatments for 24 h. The cells in Q1 to Q4 indicate dead/necrotic cells, late apoptotic/dead cells, early apoptotic cells and live cells, respectively. ( H and I ) Representative TUNEL staining images of 143B and SJSA-1 receiving different treatments for 24 h ( H ) and quantification of the apoptotic cell numbers ( I ). Scale bar: 50 µm. n = 3 per group. ( J ) CCK-8 analysis of the viability of 143B and SJSA-1 treated with solvent, F-AgÅPs or F-AgÅPs + Z-VAD-FMK for 24 h. n = 5 per group. ( K and L ) Representative images of calcein-AM/PI staining ( K ) and quantification of the percentages of live cells ( L ). Scale bar: 100 µm. n = 3 per group. ( M ) Flow cytometric analysis of Annexin V-FITC/PI-stained 143B and SJSA-1 receiving different treatments for 24 h. Data are shown as mean ± SD. * P

    Journal: Theranostics

    Article Title: Fructose-coated Angstrom silver inhibits osteosarcoma growth and metastasis via promoting ROS-dependent apoptosis through the alteration of glucose metabolism by inhibiting PDK

    doi: 10.7150/thno.45858

    Figure Lengend Snippet: F-AgÅPs induce osteosarcoma cell death through the promotion of apoptosis. ( A-D ) Representative images of TUNEL staining and quantification of the apoptotic cell numbers in tumor sections from subcutaneous 143B ( A and B ) or orthotopic SJSA-1 ( C and D ) xenografts-bearing mice treated with solvent, F-AgÅPs or cisplatin for 21 days. Scale bar: 50 µm. n = 3 per group. ( E and F ) Representative active caspase-3 staining images ( E ) and quantification of the mean staining intensity ( F ) in tumor sections from mice in ( A and B ). Scale bar: 50 µm. n = 3 per group. ( G ) Flow cytometric analysis of Annexin V-FITC/PI-stained 143B and SJSA-1 receiving different treatments for 24 h. The cells in Q1 to Q4 indicate dead/necrotic cells, late apoptotic/dead cells, early apoptotic cells and live cells, respectively. ( H and I ) Representative TUNEL staining images of 143B and SJSA-1 receiving different treatments for 24 h ( H ) and quantification of the apoptotic cell numbers ( I ). Scale bar: 50 µm. n = 3 per group. ( J ) CCK-8 analysis of the viability of 143B and SJSA-1 treated with solvent, F-AgÅPs or F-AgÅPs + Z-VAD-FMK for 24 h. n = 5 per group. ( K and L ) Representative images of calcein-AM/PI staining ( K ) and quantification of the percentages of live cells ( L ). Scale bar: 100 µm. n = 3 per group. ( M ) Flow cytometric analysis of Annexin V-FITC/PI-stained 143B and SJSA-1 receiving different treatments for 24 h. Data are shown as mean ± SD. * P

    Article Snippet: Anti-active caspase-3, anti-PCNA and the secondary antibodies were obtained from Servicebio.

    Techniques: TUNEL Assay, Staining, Mouse Assay, CCK-8 Assay

    Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo.  a  Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times.  d  H  E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P

    Journal: Clinical Epigenetics

    Article Title: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin

    doi: 10.1186/s13148-018-0605-x

    Figure Lengend Snippet: Upregulating H3K27me3 levels sensitizes OS to cisplatin in vivo. a Macroscopic image of tumor size after mice were sacrificed. Tumor volume ( b ) and weight ( c ) were measured at the indicated times. d H E, TUNEL, cleaved Caspase 3, Ki67, and CD31 staining of representative tumors harvested from mice. ** P

    Article Snippet: Immunohistochemistry After deparaffinization, rehydration, antigen retrieval, and blockade of endogenous peroxidase, tissues were incubated with anti-TUNEL (Roche), anti-cleaved Caspase 3 (Servicebio), anti-Ki67 (Servicebio), and anti-CD31 (Abways) primary antibodies at 4 °C overnight.

    Techniques: In Vivo, Mouse Assay, TUNEL Assay, Staining