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Proteintech anti cleaved caspase 3
Effects of PCNP on the apoptosis and STAT3/5 signaling pathway in human lung adenocarcinoma cells. a The apoptotic levels of A549 and H1299 cells were measured by TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining; original magnification ×100. b The percentages of TUNEL-positive cells were calculated. c Western blotting analysis for the expression of cleaved <t>caspase-3</t> and cleaved PARP in A549 and H1299 cells. GAPDH was used as the loading control. d , e The densitometry analyses of cleaved caspase-3 and cleaved PARP were performed in A549 and H1299 cells, normalized to the corresponding GAPDH level. f Western blotting analysis for the expression of p-STAT3, STAT3, p-STAT5, and STAT5 in A549 and H1299 cells. GAPDH was used as the loading control. g , h The densitometry analyses of p-STAT3, STAT3, p-STAT5, and STAT5 were performed in A549 and H1299 cells, normalized to the corresponding GAPDH level. Data are presented as mean ± SEM of three independent experiments; * P
Anti Cleaved Caspase 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cleaved caspase 3/product/Proteintech
Average 96 stars, based on 19 article reviews
Price from $9.99 to $1999.99
anti cleaved caspase 3 - by Bioz Stars, 2020-09
96/100 stars

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1) Product Images from "PEST-containing nuclear protein regulates cell proliferation, migration, and invasion in lung adenocarcinoma"

Article Title: PEST-containing nuclear protein regulates cell proliferation, migration, and invasion in lung adenocarcinoma

Journal: Oncogenesis

doi: 10.1038/s41389-019-0132-4

Effects of PCNP on the apoptosis and STAT3/5 signaling pathway in human lung adenocarcinoma cells. a The apoptotic levels of A549 and H1299 cells were measured by TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining; original magnification ×100. b The percentages of TUNEL-positive cells were calculated. c Western blotting analysis for the expression of cleaved caspase-3 and cleaved PARP in A549 and H1299 cells. GAPDH was used as the loading control. d , e The densitometry analyses of cleaved caspase-3 and cleaved PARP were performed in A549 and H1299 cells, normalized to the corresponding GAPDH level. f Western blotting analysis for the expression of p-STAT3, STAT3, p-STAT5, and STAT5 in A549 and H1299 cells. GAPDH was used as the loading control. g , h The densitometry analyses of p-STAT3, STAT3, p-STAT5, and STAT5 were performed in A549 and H1299 cells, normalized to the corresponding GAPDH level. Data are presented as mean ± SEM of three independent experiments; * P
Figure Legend Snippet: Effects of PCNP on the apoptosis and STAT3/5 signaling pathway in human lung adenocarcinoma cells. a The apoptotic levels of A549 and H1299 cells were measured by TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining; original magnification ×100. b The percentages of TUNEL-positive cells were calculated. c Western blotting analysis for the expression of cleaved caspase-3 and cleaved PARP in A549 and H1299 cells. GAPDH was used as the loading control. d , e The densitometry analyses of cleaved caspase-3 and cleaved PARP were performed in A549 and H1299 cells, normalized to the corresponding GAPDH level. f Western blotting analysis for the expression of p-STAT3, STAT3, p-STAT5, and STAT5 in A549 and H1299 cells. GAPDH was used as the loading control. g , h The densitometry analyses of p-STAT3, STAT3, p-STAT5, and STAT5 were performed in A549 and H1299 cells, normalized to the corresponding GAPDH level. Data are presented as mean ± SEM of three independent experiments; * P

Techniques Used: End Labeling, TUNEL Assay, Staining, Western Blot, Expressing

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Incubation:

Article Title: Pharmacologic Blockade of 15-PGDH Protects Against Acute Renal Injury Induced by LPS in Mice
Article Snippet: .. The membranes were blocked at room temperature for 1 h, washed with TBS-T (contains 0.1% Tween-20), and incubated with primary antibodies against 15-PGDH (1:1000 dilution, Cayman chemical, Item No.160615), caspase-3 (1:1000 dilution, proteintech, Item No.66470-2-Ig), microtubule-associated protein light chain 3 (LC-3B) (1:1000 dilution, CST, Item No. 3868), p62 (1:1000 dilution, CST, Item No. 5114), and β-actin (1:2000 dilution, Sigma-Aldrich, Item No. A1978), respectively, for overnight at 4°C. .. The membranes were then washed with TBST and incubated with secondary antibody for 1 h at RT, and signal was developed by adding the ECL luminescence substrate.

other:

Article Title: 2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury
Article Snippet: Primary antibodiesAnti-HDAC6 (sc11420, Santa Cruz), anti-acetyl-histone H3 (9649, Cell Signaling Technology), Anti-H3 (ab8580, Abcam), anti-BAX (ab32503, Abcam), anti-BCL-2 (ab3214, Abcam), anti-BCL-XL (ab32370, Abcam), anti-cleaved caspase 3 (66470-2-Ig, Proteintech Group), anti-phospho-JNK (4668, Cell Signaling Technology), anti-JNK (ab208035, Abcam), anti-phospho-eIF2α (ET1603-14, HuaBio), anti-eIF2α (RT1196, HuaBio), anti-phospho-PERK (sc32577, Santa Cruz), anti-GRP78 (ER40402, HuaBio), anti-IRE1α (ab48187, Abcam), anti-CHOP (2895, Cell Signaling Technology), anti-ATF4 (11815, Cell Signaling Technology), Anti-ATF6 (ER1706-34, HuaBio), anti-XBP1 (ab37152, Abcam), anti-caspase 12 (ab62484, Abcam).

Article Title: Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload
Article Snippet: Bax (50599-2-Ig), Bcl-2 (66799-1-Ig), and caspase-3 (66470-2-Ig) were purchased from Proteintech Group (Rosemont, USA).

Article Title: Pharmacological and genetic inhibition of fatty acid‐binding protein 4 alleviated cisplatin‐induced acute kidney injury, et al. Pharmacological and genetic inhibition of fatty acid‐binding protein 4 alleviated cisplatin‐induced acute kidney injury
Article Snippet: 2.2 Primary antibodies Primary antibodies were as follows: anti‐FABP4 (JM10‐99; HuaBio), anti‐BAX (ab32503; Abcam), anti‐BCL‐2 (ab3214; Abcam), anti‐BCL‐XL (ab32370; Abcam), anti‐cleaved caspase 3 (66470‐2‐Ig; Proteintech Group), anti‐p‐JNK (4668; Cell Signaling Technology), anti‐JNK (ab208035; Abcam), anti‐p‐eIF2α (ET1603‐14; HuaBio), anti‐eIF2α (RT1196; HuaBio), anti‐p‐PERK (sc32577; Santa Cruz), anti‐GRP78 (ER40402; HuaBio), anti‐IRE1α (ab48187; Abcam), anti‐CHOP (2895; Cell Signaling Technology), anti‐ATF4 (11815; Cell Signaling Technology), anti‐ATF6 (ER1706‐34; HuaBio), anti‐XBP1 (ab37152; Abcam) and anti‐caspase 12 (ab62484; Abcam).

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  • 92
    Proteintech anti cleaved caspase 3
    Effects of PCNP on the apoptosis of human neuroblastoma cells. a The apoptotic levels of SH-SY5Y and SK-N-SH cells were measured by TUNEL staining; original magnification 100×. b The percentages of TUNEL-positive cells were calculated by the formula shown above. c Western blotting analysis for the expression of cleaved <t>caspase-3,</t> − 8, and − 9 and cleaved PARP in SH-SY5Y and SK-N-SH cells. GAPDH was used as the loading control. ( d, e ) The densitometry analysis of each factor was performed in SH-SY5Y and SK-N-SH cells, normalized to the corresponding GAPDH level. Data are presented as mean ± SEM of three independent experiments; * P
    Anti Cleaved Caspase 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3/product/Proteintech
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Proteintech anti caspase 3
    Sal pretreatment ameliorates apoptosis and autophagy in hepatic IRI. Notes:  ( A ) The relative mRNA levels of Bcl-2, Bax, caspase 3, caspase 9, Beclin-1, LC3, and P62. ( B ) Protein expression of apoptosis- and autophagy-related proteins. ( C ) Immunohistochemistry was used to detect Bcl-2, Bax, Beclin-1, and LC3 expression in liver tissues (original magnification, ×200). The ratio of brown area to total area was analyzed with Image-Pro Plus software 6.0. ( D ) TUNEL staining showed apoptotic cells (indicated by red arrows) in the four groups 8 hours after reperfusion (original magnification, ×200). ( E ) The autophagosomes were indicated by red arrows in TEM pictures. The results showed that there was more autophagosomes formation in the IRI group than in the Sal-treated group (original magnification, ×10,000). Data were given as mean ± SD (n=6, * P
    Anti Caspase 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 3/product/Proteintech
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of PCNP on the apoptosis of human neuroblastoma cells. a The apoptotic levels of SH-SY5Y and SK-N-SH cells were measured by TUNEL staining; original magnification 100×. b The percentages of TUNEL-positive cells were calculated by the formula shown above. c Western blotting analysis for the expression of cleaved caspase-3, − 8, and − 9 and cleaved PARP in SH-SY5Y and SK-N-SH cells. GAPDH was used as the loading control. ( d, e ) The densitometry analysis of each factor was performed in SH-SY5Y and SK-N-SH cells, normalized to the corresponding GAPDH level. Data are presented as mean ± SEM of three independent experiments; * P

    Journal: BMC Cancer

    Article Title: PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways

    doi: 10.1186/s12885-018-4391-9

    Figure Lengend Snippet: Effects of PCNP on the apoptosis of human neuroblastoma cells. a The apoptotic levels of SH-SY5Y and SK-N-SH cells were measured by TUNEL staining; original magnification 100×. b The percentages of TUNEL-positive cells were calculated by the formula shown above. c Western blotting analysis for the expression of cleaved caspase-3, − 8, and − 9 and cleaved PARP in SH-SY5Y and SK-N-SH cells. GAPDH was used as the loading control. ( d, e ) The densitometry analysis of each factor was performed in SH-SY5Y and SK-N-SH cells, normalized to the corresponding GAPDH level. Data are presented as mean ± SEM of three independent experiments; * P

    Article Snippet: Anti-PCNP, Anti-B-cell lymphoma-2 (Bcl-2), anti-Bcl-2-associated X protein (Bax), anti-B-cell lymphoma-extra large (Bcl-xl), anti-Bcl-xl/Bcl-2-associated death promoter (Bad), anti-cleaved caspase-3, anti-cleaved caspase-8, anti-cleaved caspase-9, anti-Cleaved poly adenosine diphosphate-ribose polymerase (PARP), and anti-GAPDH antibodies were purchased from ProteinTech (Chicago, IL, USA).

    Techniques: TUNEL Assay, Staining, Western Blot, Expressing

    SCIN knockdown activated caspase 3 signalling pathway and induced cytochrome c .

    Journal: FEBS Open Bio

    Article Title: Loss of scinderin decreased expression of epidermal growth factor receptor and promoted apoptosis of castration‐resistant prostate cancer cells

    doi: 10.1002/2211-5463.12412

    Figure Lengend Snippet: SCIN knockdown activated caspase 3 signalling pathway and induced cytochrome c .

    Article Snippet: After blocking the membrane with 5% non‐fat milk, target proteins were detected using the following antibodies: anti‐SCIN, anti‐epidermal growth factor receptor (EGFR) anti‐B‐cell lymphoma‐extra‐large (Bcl‐xl), anti‐caspase 9, anti‐cleaved caspase 3, anti‐cytochrome c (all 1 : 1000; Proteintech, Rosemont, IL, USA), anti‐ glyceraldehyde 3‐phosphate dehydrogenase (GAPDH, 1 : 500 000; all Proteintech), anti‐mitogen‐activated protein kinase kinase (MEK), anti‐phosphorylated (p)‐MEK (both 1 : 1000; SAB, College Park, MD, USA), anti‐extracellular signal‐regulated kinase (ERK), anti‐p‐ERK (both 1 : 3000; Santa Cruz Biotechnology, Dallas, TX, USA), and anti‐Akt (1 : 1000; Cell Signaling Technology, Danvers, MA, USA), anti‐p‐AKT, anti‐poly‐ADP ribose polymerase (PARP; both 1 : 1000; Cell Signaling Technology).

    Techniques:

    miR-2053 promotes apoptosis in Huh7 cells. (A) After 24 h of transfection, cell apoptosis was measured by flow cytometry after incubation with FITC-Annexin V and PI solution. (B) Percentage of cells positive for Annexin V and PI. (C) Protein expression levels of BCL2, BAX, caspase-9 and cleaved caspase-3. *P

    Journal: Oncology Letters

    Article Title: MicroRNA-2053 overexpression inhibits the development and progression of hepatocellular carcinoma

    doi: 10.3892/ol.2019.10501

    Figure Lengend Snippet: miR-2053 promotes apoptosis in Huh7 cells. (A) After 24 h of transfection, cell apoptosis was measured by flow cytometry after incubation with FITC-Annexin V and PI solution. (B) Percentage of cells positive for Annexin V and PI. (C) Protein expression levels of BCL2, BAX, caspase-9 and cleaved caspase-3. *P

    Article Snippet: The following primary antibodies were used for analysis: Anti-caspase-9 (cat. no. ab219590; 1:1,000), anti-AKT (cat. no. ab32505, 1:1,000), anti-phosphorylated AKT (cat. no. ab81283, 1:1,000), anti-mTOR (cat. no. ab2732, 1:1,000), anti-phosphorylated mTOR (cat. no. ab131538, 1:100), anti-Wnt3 (cat. no. ab32249, 1:10), anti-β-catenin (cat. no. ab32572, 1:1,000, all Abcam, Cambridge, UK), anti-E-cadherin (cat. no. 3195; 1:1,000; Cell Signaling Technology, Danvers, MA, USA), anti-Cyclin D1 (cat. no. 60186-1-Ig, 1:1,000), anti-p70 (cat. no. 14485-1-AP, 1:1,000), anti-B-cell lymphoma 2 (BCL2; cat. no. 60178-1-Ig, 1:1,000), anti-BCL-2-associated X protein (BAX; cat. no. 60267-1-Ig, 1:1,000) and anti-cleaved caspase-3 (cat. no. 25546-1-AP, 1:1,000) and GAPDH (cat. no. 60004-1-Ig, 1:5,000; all Proteintech Group, Inc.), which served as the loading control.

    Techniques: Transfection, Flow Cytometry, Cytometry, Incubation, Expressing

    Sal pretreatment ameliorates apoptosis and autophagy in hepatic IRI. Notes:  ( A ) The relative mRNA levels of Bcl-2, Bax, caspase 3, caspase 9, Beclin-1, LC3, and P62. ( B ) Protein expression of apoptosis- and autophagy-related proteins. ( C ) Immunohistochemistry was used to detect Bcl-2, Bax, Beclin-1, and LC3 expression in liver tissues (original magnification, ×200). The ratio of brown area to total area was analyzed with Image-Pro Plus software 6.0. ( D ) TUNEL staining showed apoptotic cells (indicated by red arrows) in the four groups 8 hours after reperfusion (original magnification, ×200). ( E ) The autophagosomes were indicated by red arrows in TEM pictures. The results showed that there was more autophagosomes formation in the IRI group than in the Sal-treated group (original magnification, ×10,000). Data were given as mean ± SD (n=6, * P

    Journal: Drug Design, Development and Therapy

    Article Title: Salidroside pretreatment attenuates apoptosis and autophagy during hepatic ischemia–reperfusion injury by inhibiting the mitogen-activated protein kinase pathway in mice

    doi: 10.2147/DDDT.S136792

    Figure Lengend Snippet: Sal pretreatment ameliorates apoptosis and autophagy in hepatic IRI. Notes: ( A ) The relative mRNA levels of Bcl-2, Bax, caspase 3, caspase 9, Beclin-1, LC3, and P62. ( B ) Protein expression of apoptosis- and autophagy-related proteins. ( C ) Immunohistochemistry was used to detect Bcl-2, Bax, Beclin-1, and LC3 expression in liver tissues (original magnification, ×200). The ratio of brown area to total area was analyzed with Image-Pro Plus software 6.0. ( D ) TUNEL staining showed apoptotic cells (indicated by red arrows) in the four groups 8 hours after reperfusion (original magnification, ×200). ( E ) The autophagosomes were indicated by red arrows in TEM pictures. The results showed that there was more autophagosomes formation in the IRI group than in the Sal-treated group (original magnification, ×10,000). Data were given as mean ± SD (n=6, * P

    Article Snippet: The primary antibodies used in this study were anti-Bax, anti-caspase 3, anti-caspase 9, anti-Beclin-1, anti-P62, anti-JNK, anti-P38, anti-p-P38 (Proteintech, Chicago, IL, USA), anti-TNF-α, anti-Bcl-2, antimicrotubule-associated protein 1 light chain 3 (LC3), antimammalian target of rapamycin (mTOR), anti-p-mTOR, anti-Akt, anti-p-Akt, anti-S6, anti-p-S6 (Cell Signaling Technology, Danvers, MA, USA), anti-IL-6, anti-p-JNK (Antibody Revolution, San Diego, CA, USA), anti-ERK, and anti-p-ERK (Signalway Antibody, College Park, MD, USA).

    Techniques: Expressing, Immunohistochemistry, Software, TUNEL Assay, Staining, Transmission Electron Microscopy