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Merck KGaA anti cleaved caspase 3
P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, <t>Caspase</t> 3 were measured. *P
Anti Cleaved Caspase 3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS"

Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS

Journal: Cell Cycle

doi: 10.1080/15384101.2018.1542900

P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, Caspase 3 were measured. *P
Figure Legend Snippet: P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, Caspase 3 were measured. *P

Techniques Used: Isolation, Flow Cytometry, Cytometry

CBS expression, HK-2 cell apoptosis and apoptosis related proteins in allograft renal transplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+miR-191 inhibitor (800 pmol), and model+miR-191 inhibitor (800 pmol). mRNA and protein levels of CBS (a), HK-2 cell apoptosis (b) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (c) were measured. CBS expression, HK-2 cell apoptosis and apoptosis related proteins in renal autotransplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+P-MVs (200 ug), and model+P-MVs (200 ug). mRNA and protein levels of CBS (d), HK-2 cell apoptosis (e) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (f) were measured. *P
Figure Legend Snippet: CBS expression, HK-2 cell apoptosis and apoptosis related proteins in allograft renal transplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+miR-191 inhibitor (800 pmol), and model+miR-191 inhibitor (800 pmol). mRNA and protein levels of CBS (a), HK-2 cell apoptosis (b) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (c) were measured. CBS expression, HK-2 cell apoptosis and apoptosis related proteins in renal autotransplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+P-MVs (200 ug), and model+P-MVs (200 ug). mRNA and protein levels of CBS (d), HK-2 cell apoptosis (e) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (f) were measured. *P

Techniques Used: Expressing, Transplantation Assay

2) Product Images from "miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS"

Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS

Journal: Cell Cycle

doi: 10.1080/15384101.2018.1542900

P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, Caspase 3 were measured. *P
Figure Legend Snippet: P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, Caspase 3 were measured. *P

Techniques Used: Isolation, Flow Cytometry, Cytometry

CBS expression, HK-2 cell apoptosis and apoptosis related proteins in allograft renal transplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+miR-191 inhibitor (800 pmol), and model+miR-191 inhibitor (800 pmol). mRNA and protein levels of CBS (a), HK-2 cell apoptosis (b) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (c) were measured. CBS expression, HK-2 cell apoptosis and apoptosis related proteins in renal autotransplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+P-MVs (200 ug), and model+P-MVs (200 ug). mRNA and protein levels of CBS (d), HK-2 cell apoptosis (e) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (f) were measured. *P
Figure Legend Snippet: CBS expression, HK-2 cell apoptosis and apoptosis related proteins in allograft renal transplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+miR-191 inhibitor (800 pmol), and model+miR-191 inhibitor (800 pmol). mRNA and protein levels of CBS (a), HK-2 cell apoptosis (b) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (c) were measured. CBS expression, HK-2 cell apoptosis and apoptosis related proteins in renal autotransplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+P-MVs (200 ug), and model+P-MVs (200 ug). mRNA and protein levels of CBS (d), HK-2 cell apoptosis (e) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (f) were measured. *P

Techniques Used: Expressing, Transplantation Assay

3) Product Images from "Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells"

Article Title: Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells

Journal: Cell Death & Disease

doi: 10.1038/s41419-018-0537-6

IDH2 deficiency exacerbates apoptosis after cisplatin administration. Idh2 ‒/‒ mice and wild-type ( Idh2 +/+ ) littermates were intraperitoneally injected with either cisplatin (C, 20 mg/kg B.W.) or 0.9% saline (vehicle, V) once. Some mice were treated with Mito-T (M, 0.7 mg/kg B.W.) daily, beginning 7 days before cisplatin injection and continuing until experiments were completed. Kidneys were harvested 3 days after cisplatin injection. a – c Bax and Bcl-2 expression in whole kidney lysates were determined by western blot analysis. GAPDH was used as a loading control. b , c Bax ( b ) and Bcl-2 ( c ) band densities were measured using the ImageJ program. d – f Mitochondria and cytosol were fractioned as described in the “Materials and methods” section. Cytochrome c expression was determined in mitochondrial and cytosolic fractions by western blot analysis. e , f Band densities were measured using the ImageJ program and normalized to COX IV and GAPDH. g Cleaved caspase-3 was detected in whole kidney lysates by western blot analysis. h Band density was measured using the ImageJ program and normalized to GAPDH. Results are expressed as means ± SE ( n = 3–5 per group). * p
Figure Legend Snippet: IDH2 deficiency exacerbates apoptosis after cisplatin administration. Idh2 ‒/‒ mice and wild-type ( Idh2 +/+ ) littermates were intraperitoneally injected with either cisplatin (C, 20 mg/kg B.W.) or 0.9% saline (vehicle, V) once. Some mice were treated with Mito-T (M, 0.7 mg/kg B.W.) daily, beginning 7 days before cisplatin injection and continuing until experiments were completed. Kidneys were harvested 3 days after cisplatin injection. a – c Bax and Bcl-2 expression in whole kidney lysates were determined by western blot analysis. GAPDH was used as a loading control. b , c Bax ( b ) and Bcl-2 ( c ) band densities were measured using the ImageJ program. d – f Mitochondria and cytosol were fractioned as described in the “Materials and methods” section. Cytochrome c expression was determined in mitochondrial and cytosolic fractions by western blot analysis. e , f Band densities were measured using the ImageJ program and normalized to COX IV and GAPDH. g Cleaved caspase-3 was detected in whole kidney lysates by western blot analysis. h Band density was measured using the ImageJ program and normalized to GAPDH. Results are expressed as means ± SE ( n = 3–5 per group). * p

Techniques Used: Mouse Assay, Injection, Expressing, Western Blot

4) Product Images from "ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway"

Article Title: ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2018/6451902

ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P
Figure Legend Snippet: ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P

Techniques Used: Mouse Assay, TUNEL Assay, Staining, Western Blot

ZP2495 exerted antiapoptotic effect on high-glucose-induced NRVMs after SI/R injury. (a) Apoptosis of the NRVMs determined by annexin V/propidium iodide (PI) double staining and flow cytometry. Region Q2 late apoptotic cells, region Q3 vital cells, and region Q4 early apoptotic cells. (b) Representative images of immunostaining for apoptotic (TUNEL) cells (scale bar = 100 μ m). (c) Quantification of apoptosis cell by flow cytometry and TUNEL staining. (d) Quantification of apoptosis cell by TUNEL staining. (e) Activities of caspase-3. Con: normal glucose medium group; HG: high-glucose medium group; HG + SI/R: HG + SI/R group; ZP2495: HG + SI/R + ZP2495 group; glucagon: HG + SI/R + glucagon group; and ZP131: HG + SI/R + ZP131 group. Presented values are mean ± SEM. † P
Figure Legend Snippet: ZP2495 exerted antiapoptotic effect on high-glucose-induced NRVMs after SI/R injury. (a) Apoptosis of the NRVMs determined by annexin V/propidium iodide (PI) double staining and flow cytometry. Region Q2 late apoptotic cells, region Q3 vital cells, and region Q4 early apoptotic cells. (b) Representative images of immunostaining for apoptotic (TUNEL) cells (scale bar = 100 μ m). (c) Quantification of apoptosis cell by flow cytometry and TUNEL staining. (d) Quantification of apoptosis cell by TUNEL staining. (e) Activities of caspase-3. Con: normal glucose medium group; HG: high-glucose medium group; HG + SI/R: HG + SI/R group; ZP2495: HG + SI/R + ZP2495 group; glucagon: HG + SI/R + glucagon group; and ZP131: HG + SI/R + ZP131 group. Presented values are mean ± SEM. † P

Techniques Used: Double Staining, Flow Cytometry, Cytometry, Immunostaining, TUNEL Assay, Staining

5) Product Images from "Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells"

Article Title: Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells

Journal: Cell Death & Disease

doi: 10.1038/s41419-018-0537-6

IDH2 deficiency exacerbates apoptosis after cisplatin administration. Idh2 ‒/‒ mice and wild-type ( Idh2 +/+ ) littermates were intraperitoneally injected with either cisplatin (C, 20 mg/kg B.W.) or 0.9% saline (vehicle, V) once. Some mice were treated with Mito-T (M, 0.7 mg/kg B.W.) daily, beginning 7 days before cisplatin injection and continuing until experiments were completed. Kidneys were harvested 3 days after cisplatin injection. a – c Bax and Bcl-2 expression in whole kidney lysates were determined by western blot analysis. GAPDH was used as a loading control. b , c Bax ( b ) and Bcl-2 ( c ) band densities were measured using the ImageJ program. d – f Mitochondria and cytosol were fractioned as described in the “Materials and methods” section. Cytochrome c expression was determined in mitochondrial and cytosolic fractions by western blot analysis. e , f Band densities were measured using the ImageJ program and normalized to COX IV and GAPDH. g Cleaved caspase-3 was detected in whole kidney lysates by western blot analysis. h Band density was measured using the ImageJ program and normalized to GAPDH. Results are expressed as means ± SE ( n = 3–5 per group). * p
Figure Legend Snippet: IDH2 deficiency exacerbates apoptosis after cisplatin administration. Idh2 ‒/‒ mice and wild-type ( Idh2 +/+ ) littermates were intraperitoneally injected with either cisplatin (C, 20 mg/kg B.W.) or 0.9% saline (vehicle, V) once. Some mice were treated with Mito-T (M, 0.7 mg/kg B.W.) daily, beginning 7 days before cisplatin injection and continuing until experiments were completed. Kidneys were harvested 3 days after cisplatin injection. a – c Bax and Bcl-2 expression in whole kidney lysates were determined by western blot analysis. GAPDH was used as a loading control. b , c Bax ( b ) and Bcl-2 ( c ) band densities were measured using the ImageJ program. d – f Mitochondria and cytosol were fractioned as described in the “Materials and methods” section. Cytochrome c expression was determined in mitochondrial and cytosolic fractions by western blot analysis. e , f Band densities were measured using the ImageJ program and normalized to COX IV and GAPDH. g Cleaved caspase-3 was detected in whole kidney lysates by western blot analysis. h Band density was measured using the ImageJ program and normalized to GAPDH. Results are expressed as means ± SE ( n = 3–5 per group). * p

Techniques Used: Mouse Assay, Injection, Expressing, Western Blot

6) Product Images from "ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway"

Article Title: ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2018/6451902

ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P
Figure Legend Snippet: ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P

Techniques Used: Mouse Assay, TUNEL Assay, Staining, Western Blot

ZP2495 exerted antiapoptotic effect on high-glucose-induced NRVMs after SI/R injury. (a) Apoptosis of the NRVMs determined by annexin V/propidium iodide (PI) double staining and flow cytometry. Region Q2 late apoptotic cells, region Q3 vital cells, and region Q4 early apoptotic cells. (b) Representative images of immunostaining for apoptotic (TUNEL) cells (scale bar = 100 μ m). (c) Quantification of apoptosis cell by flow cytometry and TUNEL staining. (d) Quantification of apoptosis cell by TUNEL staining. (e) Activities of caspase-3. Con: normal glucose medium group; HG: high-glucose medium group; HG + SI/R: HG + SI/R group; ZP2495: HG + SI/R + ZP2495 group; glucagon: HG + SI/R + glucagon group; and ZP131: HG + SI/R + ZP131 group. Presented values are mean ± SEM. † P
Figure Legend Snippet: ZP2495 exerted antiapoptotic effect on high-glucose-induced NRVMs after SI/R injury. (a) Apoptosis of the NRVMs determined by annexin V/propidium iodide (PI) double staining and flow cytometry. Region Q2 late apoptotic cells, region Q3 vital cells, and region Q4 early apoptotic cells. (b) Representative images of immunostaining for apoptotic (TUNEL) cells (scale bar = 100 μ m). (c) Quantification of apoptosis cell by flow cytometry and TUNEL staining. (d) Quantification of apoptosis cell by TUNEL staining. (e) Activities of caspase-3. Con: normal glucose medium group; HG: high-glucose medium group; HG + SI/R: HG + SI/R group; ZP2495: HG + SI/R + ZP2495 group; glucagon: HG + SI/R + glucagon group; and ZP131: HG + SI/R + ZP131 group. Presented values are mean ± SEM. † P

Techniques Used: Double Staining, Flow Cytometry, Cytometry, Immunostaining, TUNEL Assay, Staining

Related Articles

Western Blot:

Article Title: Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells
Article Snippet: .. Western blotting was performed using anti-4-hydroxynonenal (4-HNE; Abcam), anti-peroxiredoxin (Prx-SO3 , Abcam), anti-MnSOD (Calbiochem, San Diego, CA), anti-copper-zinc superoxide dismutase (CuZnSOD; Chemicon, Temecula, CA), anti-histone H1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-mitochondrial NADP+ -dependent isocitrate dehydrogenase (IDH2) , anti-Opa1 (BD Bioscience, Franklin Lakes, NJ, USA), anti-Drp1 (Cell Signaling Technology, Danvers, MA, USA), anti-Bax (5B7; Santa Cruz), anti-Bcl-2 (Cell Signaling Technology), anti-cytochrome c (BD Bioscience), anti-cleaved caspase-3 (Merck Millipore, Darmstadt, Germany), anti-β-actin (Sigma), and anti-GAPDH (Novus, Littleton, CO, USA) antibodies. .. The determinations of IDH1 and IDH2, and IDH3 activity were based on the productions of NADPH and NADH, respectively .

Article Title: Mitochondrial NADP+-dependent isocitrate dehydrogenase deficiency increases cisplatin-induced oxidative damage in the kidney tubule cells
Article Snippet: .. Western blot analysis Western blotting was performed using anti-4-hydroxynonenal (4-HNE; Abcam), anti-peroxiredoxin (Prx-SO3 , Abcam), anti-MnSOD (Calbiochem, San Diego, CA), anti-copper-zinc superoxide dismutase (CuZnSOD; Chemicon, Temecula, CA), anti-histone H1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-mitochondrial NADP+ -dependent isocitrate dehydrogenase (IDH2) , anti-Opa1 (BD Bioscience, Franklin Lakes, NJ, USA), anti-Drp1 (Cell Signaling Technology, Danvers, MA, USA), anti-Bax (5B7; Santa Cruz), anti-Bcl-2 (Cell Signaling Technology), anti-cytochrome c (BD Bioscience), anti-cleaved caspase-3 (Merck Millipore, Darmstadt, Germany), anti-β-actin (Sigma), and anti-GAPDH (Novus, Littleton, CO, USA) antibodies. .. Measurement of IDH1, IDH2, and IDH3 activity The determinations of IDH1 and IDH2, and IDH3 activity were based on the productions of NADPH and NADH, respectively .

SDS Page:

Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS
Article Snippet: .. Protein samples were isolated on 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Invitrogen, USA) which were blocked in 5% skim milk for 2 h. Anti-CBS (Invitrogen), anti-cleaved caspase 3 (Merck Millipore), anti-BAX (Invitrogen), anti-Bcl2 (Invitrogen) and anti-β-Actin were used as the first primary antibody at 1:1000 dilutions. .. Then, cultivalted with secondary antibody (corresponding horseradish peroxidase-conjugated) at room temperature for 1 h. Protein-antibody complexes were detected by ECL System (GE Healthcare, UK).

Isolation:

Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS
Article Snippet: .. Protein samples were isolated on 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Invitrogen, USA) which were blocked in 5% skim milk for 2 h. Anti-CBS (Invitrogen), anti-cleaved caspase 3 (Merck Millipore), anti-BAX (Invitrogen), anti-Bcl2 (Invitrogen) and anti-β-Actin were used as the first primary antibody at 1:1000 dilutions. .. Then, cultivalted with secondary antibody (corresponding horseradish peroxidase-conjugated) at room temperature for 1 h. Protein-antibody complexes were detected by ECL System (GE Healthcare, UK).

Nucleic Acid Electrophoresis:

Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS
Article Snippet: .. Protein samples were isolated on 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Invitrogen, USA) which were blocked in 5% skim milk for 2 h. Anti-CBS (Invitrogen), anti-cleaved caspase 3 (Merck Millipore), anti-BAX (Invitrogen), anti-Bcl2 (Invitrogen) and anti-β-Actin were used as the first primary antibody at 1:1000 dilutions. .. Then, cultivalted with secondary antibody (corresponding horseradish peroxidase-conjugated) at room temperature for 1 h. Protein-antibody complexes were detected by ECL System (GE Healthcare, UK).

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    Merck KGaA rabbit anti active casp3 antibody
    Adult hippocampal neurogenesis was analyzed using immunofluorescence staining within the dentate gyrus (DG) of the hippocampus. Cell counts were made using Abercrombie correction. Golgi-impregnated material was used to reconstruct dendrites in area CA1 and to analyze dendritic spine densities. (A) Leptin-deficient (ob/ob) mice showed a reduced amount of PH3-positive proliferating cells. (B) The ob/ob mice show a reduced number of DCX-positive cells in the DG compared with wild-type (wt) controls. (B′) Example of DCX-stained neurons within the DG. Images were observed using an Olympus BX63 microscope with a 40× objective. (C) As an apoptosis marker for apoptotic events, caspase3 <t>(Casp3)</t> was used. The number of Casp3-positive cells did not differ between the 2 groups of mice. (D) No difference in spine densities of apical dendrites of hippocampal CA1 pyramidal neurons was noted by comparing age-matched control (wt) or obese (ob/ob) mice. (E) Spine densities of basal dendrites of hippocampal CA1 pyramidal neurons were also not significantly different. DCX indicates doublecortin; PH3, phosphohistone H3.
    Rabbit Anti Active Casp3 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti active casp3 antibody/product/Merck KGaA
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    Merck KGaA rabbit anti human caspase 3
    ZIKV reduces the growth of neurospheres, by depleting the pool of neural progenitors and the generation of neurons. ( A–F ) Immunocytochemistry for the flavivirus antigen (red) and cleaved <t>caspase-3</t> (green) counterstained with DAPI (blue) on Mock- and ZIKV-infected neurospheres. ( G ) Quantification of flavivirus antigen and ( H ) cleaved caspase-3 fluorescence intensity. Individual sample data were normalized against the average of Mock-infected experimental group. ( I–P ) Immunocytochemistry for the neuronal marker HuC/D (red) and the neural progenitor marker SOX2 (green) counterstained with DAPI (blue). Quantification of HuC/D and SOX2 fluorescence intensity shows a decrease of both markers on ZIKV-infected neurospheres. ( Q,R ) Flow cytometry distribution analysis of neurospheres at subG1 phase of cell cycle 3 days after ZIKV or mock infection. Differences on bar graphs were expressed by fold change in relation to the average values of the Mock-infected group. Data presented as mean ± SD, n = 4, Student’s t-test, *p
    Rabbit Anti Human Caspase 3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human caspase 3/product/Merck KGaA
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    Price from $9.99 to $1999.99
    rabbit anti human caspase 3 - by Bioz Stars, 2020-09
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    93
    Merck KGaA anti cleaved caspase 3
    P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, <t>Caspase</t> 3 were measured. *P
    Anti Cleaved Caspase 3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3/product/Merck KGaA
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    Merck KGaA anti caspase 3
    ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of <t>caspase-3</t> and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P
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    Adult hippocampal neurogenesis was analyzed using immunofluorescence staining within the dentate gyrus (DG) of the hippocampus. Cell counts were made using Abercrombie correction. Golgi-impregnated material was used to reconstruct dendrites in area CA1 and to analyze dendritic spine densities. (A) Leptin-deficient (ob/ob) mice showed a reduced amount of PH3-positive proliferating cells. (B) The ob/ob mice show a reduced number of DCX-positive cells in the DG compared with wild-type (wt) controls. (B′) Example of DCX-stained neurons within the DG. Images were observed using an Olympus BX63 microscope with a 40× objective. (C) As an apoptosis marker for apoptotic events, caspase3 (Casp3) was used. The number of Casp3-positive cells did not differ between the 2 groups of mice. (D) No difference in spine densities of apical dendrites of hippocampal CA1 pyramidal neurons was noted by comparing age-matched control (wt) or obese (ob/ob) mice. (E) Spine densities of basal dendrites of hippocampal CA1 pyramidal neurons were also not significantly different. DCX indicates doublecortin; PH3, phosphohistone H3.

    Journal: Journal of Experimental Neuroscience

    Article Title: Obesity Impairs Mobility and Adult Hippocampal Neurogenesis

    doi: 10.1177/1179069519883580

    Figure Lengend Snippet: Adult hippocampal neurogenesis was analyzed using immunofluorescence staining within the dentate gyrus (DG) of the hippocampus. Cell counts were made using Abercrombie correction. Golgi-impregnated material was used to reconstruct dendrites in area CA1 and to analyze dendritic spine densities. (A) Leptin-deficient (ob/ob) mice showed a reduced amount of PH3-positive proliferating cells. (B) The ob/ob mice show a reduced number of DCX-positive cells in the DG compared with wild-type (wt) controls. (B′) Example of DCX-stained neurons within the DG. Images were observed using an Olympus BX63 microscope with a 40× objective. (C) As an apoptosis marker for apoptotic events, caspase3 (Casp3) was used. The number of Casp3-positive cells did not differ between the 2 groups of mice. (D) No difference in spine densities of apical dendrites of hippocampal CA1 pyramidal neurons was noted by comparing age-matched control (wt) or obese (ob/ob) mice. (E) Spine densities of basal dendrites of hippocampal CA1 pyramidal neurons were also not significantly different. DCX indicates doublecortin; PH3, phosphohistone H3.

    Article Snippet: After rinsing in PBS, sections were incubated in a solution (3% NGS + 0.1% Triton X-100 in PBS) containing the primary rabbit anti-active Casp3 antibody (Merck Millipore, Germany; 1:100) at 4°C overnight.

    Techniques: Immunofluorescence, Staining, Mouse Assay, Microscopy, Marker

    ZIKV reduces the growth of neurospheres, by depleting the pool of neural progenitors and the generation of neurons. ( A–F ) Immunocytochemistry for the flavivirus antigen (red) and cleaved caspase-3 (green) counterstained with DAPI (blue) on Mock- and ZIKV-infected neurospheres. ( G ) Quantification of flavivirus antigen and ( H ) cleaved caspase-3 fluorescence intensity. Individual sample data were normalized against the average of Mock-infected experimental group. ( I–P ) Immunocytochemistry for the neuronal marker HuC/D (red) and the neural progenitor marker SOX2 (green) counterstained with DAPI (blue). Quantification of HuC/D and SOX2 fluorescence intensity shows a decrease of both markers on ZIKV-infected neurospheres. ( Q,R ) Flow cytometry distribution analysis of neurospheres at subG1 phase of cell cycle 3 days after ZIKV or mock infection. Differences on bar graphs were expressed by fold change in relation to the average values of the Mock-infected group. Data presented as mean ± SD, n = 4, Student’s t-test, *p

    Journal: Scientific Reports

    Article Title: Zika virus disrupts molecular fingerprinting of human neurospheres

    doi: 10.1038/srep40780

    Figure Lengend Snippet: ZIKV reduces the growth of neurospheres, by depleting the pool of neural progenitors and the generation of neurons. ( A–F ) Immunocytochemistry for the flavivirus antigen (red) and cleaved caspase-3 (green) counterstained with DAPI (blue) on Mock- and ZIKV-infected neurospheres. ( G ) Quantification of flavivirus antigen and ( H ) cleaved caspase-3 fluorescence intensity. Individual sample data were normalized against the average of Mock-infected experimental group. ( I–P ) Immunocytochemistry for the neuronal marker HuC/D (red) and the neural progenitor marker SOX2 (green) counterstained with DAPI (blue). Quantification of HuC/D and SOX2 fluorescence intensity shows a decrease of both markers on ZIKV-infected neurospheres. ( Q,R ) Flow cytometry distribution analysis of neurospheres at subG1 phase of cell cycle 3 days after ZIKV or mock infection. Differences on bar graphs were expressed by fold change in relation to the average values of the Mock-infected group. Data presented as mean ± SD, n = 4, Student’s t-test, *p

    Article Snippet: Incubation with rabbit anti-human-Sox2 (1:100; Merck-Millipore, Germany), rabbit anti-human-caspase 3 active (cleaved) form (1:100; Merck-Millipore, Germany), rabbit anti-GFAP (1:100); mouse anti-human-HuC/HuD neuronal protein (16A11) (1:100, Invitrogen, USA), mouse anti-H2aX (1:100), mouse anti-flavivirus group antigen antibody (clone 4G2, 1:100), rabbit anti-PAX6 (1:100, Santa Cruz Biotechnologies, USA) mouse anti-TLR4 (H-80) (1:100, Santa Cruz, USA), rabbit anti-Vimentin (1:500, abcam, USA) and mouse anti-phospho (S55) Vimentin (1:250, abcam, USA) was performed overnight.

    Techniques: Immunocytochemistry, Infection, Fluorescence, Marker, Flow Cytometry, Cytometry

    P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, Caspase 3 were measured. *P

    Journal: Cell Cycle

    Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS

    doi: 10.1080/15384101.2018.1542900

    Figure Lengend Snippet: P-MVs-secreted miR-191 affected tubular epithelial cell apoptosis via CBS. P-MVs isolated from patients with renal allograft rejection were divided into seven groups: control, P-MVs of healthy, P-MVs of patients, P-MVs of patients+NC, P-MVs of patients+miR-191 Inhibitor, P-MVs of patients+miR-191 inhibitor+si-control, P-MVs of patients+miR-191 Inhibitor+si-CBS. A. HK-2 cell apoptosis was observed by flow cytometry. B. Apoptosis related proteins of Bax, Bcl-2, Caspase 3 were measured. *P

    Article Snippet: Protein samples were isolated on 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Invitrogen, USA) which were blocked in 5% skim milk for 2 h. Anti-CBS (Invitrogen), anti-cleaved caspase 3 (Merck Millipore), anti-BAX (Invitrogen), anti-Bcl2 (Invitrogen) and anti-β-Actin were used as the first primary antibody at 1:1000 dilutions.

    Techniques: Isolation, Flow Cytometry, Cytometry

    CBS expression, HK-2 cell apoptosis and apoptosis related proteins in allograft renal transplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+miR-191 inhibitor (800 pmol), and model+miR-191 inhibitor (800 pmol). mRNA and protein levels of CBS (a), HK-2 cell apoptosis (b) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (c) were measured. CBS expression, HK-2 cell apoptosis and apoptosis related proteins in renal autotransplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+P-MVs (200 ug), and model+P-MVs (200 ug). mRNA and protein levels of CBS (d), HK-2 cell apoptosis (e) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (f) were measured. *P

    Journal: Cell Cycle

    Article Title: miR-191 secreted by platelet-derived microvesicles induced apoptosis of renal tubular epithelial cells and participated in renal ischemia-reperfusion injury via inhibiting CBS

    doi: 10.1080/15384101.2018.1542900

    Figure Lengend Snippet: CBS expression, HK-2 cell apoptosis and apoptosis related proteins in allograft renal transplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+miR-191 inhibitor (800 pmol), and model+miR-191 inhibitor (800 pmol). mRNA and protein levels of CBS (a), HK-2 cell apoptosis (b) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (c) were measured. CBS expression, HK-2 cell apoptosis and apoptosis related proteins in renal autotransplantation rat model. Rats were divided into four groups: sham+PBS, model+PBS, sham+P-MVs (200 ug), and model+P-MVs (200 ug). mRNA and protein levels of CBS (d), HK-2 cell apoptosis (e) apoptosis related proteins of Bax, Bcl-2, Caspase 3 (f) were measured. *P

    Article Snippet: Protein samples were isolated on 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Invitrogen, USA) which were blocked in 5% skim milk for 2 h. Anti-CBS (Invitrogen), anti-cleaved caspase 3 (Merck Millipore), anti-BAX (Invitrogen), anti-Bcl2 (Invitrogen) and anti-β-Actin were used as the first primary antibody at 1:1000 dilutions.

    Techniques: Expressing, Transplantation Assay

    ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway

    doi: 10.1155/2018/6451902

    Figure Lengend Snippet: ZP2495 exerted antiapoptotic effect on cardiomyocyte of db/db mice. (a) Representative photomicrograph shows TUNEL-positive cardiomyocyte ratio, which was significantly decreased in the ZP2495 group. Apoptotic nuclei were identified as TUNEL positive (green). Myocardium was stained using a monoclonal antibody against troponin I (cTnI) (red) and total nuclei by DAPI counterstaining (blue) (scale bar = 100 μ m). (b) Quantitative analysis of apoptotic nuclei. Apoptotic index was termed as the percentage of apoptotic cells. (c) Representative blots of caspase-3 and cleaved caspase-3 in cardiomyocyte of db/db mice subjected to I/R. (d) Semiquantitative analysis of the Western blots. Sham: BKS + sham group; db/db: db/db + sham group; I/R: db/db + I/R group; ZP2495: db/db + I/R + ZP2495 group; glucagon: db/db + I/R + glucagon group; and ZP131: db/db + I/R + ZP131 group. Presented values are mean ± SEM. ∗ P

    Article Snippet: The following primary antibodies were used: anti-phospho-FoxO3a (Thr32) (rabbit polyclonal IgG, Abcam, 1 : 100); anti-phospho-FoxO3a (Ser413) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-FoxO3a (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-Akt (Ser473) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-Akt (Thr308) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-Akt (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-AMPK (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-AMPK (Thr172) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-caspase-3 (rabbit polyclonal IgG, Merck Millipore, 1 : 200); anti-cleaved caspase-3 (rabbit polyclonal IgG, Merck Millipore, 1 : 200); anti-Bim (rabbit polyclonal IgG, Abcam, 1 : 1000); anti-Bax (rabbit polyclonal IgG, Abcam, 1 : 1000); anti-Bcl-2 (rabbit monoclonal IgG, Abcam, 1 : 500); anti-Bad (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-Bad (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-MnSOD (rabbit polyclonal IgG, Abcam, 1 : 5000); anti-Catalase (rabbit polyclonal IgG, Abcam, 1 : 2000); anti-Sirt1 (rabbit polyclonal IgG, Abcam, 1 : 1000); anti-PGC-1α (rabbit polyclonal IgG, Abcam, 1 : 1000); acetylated-lysine (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000,); anti-Nrf-1 (rabbit monoclonal IgG, Abcam, 1 : 5000); anti-Tfam (rabbit polyclonal IgG, Abcam, 1 : 2000); and β -actin (rabbit polyclonal IgG, Santa Cruz Biotechnology, 1 : 1000).

    Techniques: Mouse Assay, TUNEL Assay, Staining, Western Blot

    ZP2495 exerted antiapoptotic effect on high-glucose-induced NRVMs after SI/R injury. (a) Apoptosis of the NRVMs determined by annexin V/propidium iodide (PI) double staining and flow cytometry. Region Q2 late apoptotic cells, region Q3 vital cells, and region Q4 early apoptotic cells. (b) Representative images of immunostaining for apoptotic (TUNEL) cells (scale bar = 100 μ m). (c) Quantification of apoptosis cell by flow cytometry and TUNEL staining. (d) Quantification of apoptosis cell by TUNEL staining. (e) Activities of caspase-3. Con: normal glucose medium group; HG: high-glucose medium group; HG + SI/R: HG + SI/R group; ZP2495: HG + SI/R + ZP2495 group; glucagon: HG + SI/R + glucagon group; and ZP131: HG + SI/R + ZP131 group. Presented values are mean ± SEM. † P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: ZP2495 Protects against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice through Improvement of Cardiac Metabolism and Mitochondrial Function: The Possible Involvement of AMPK-FoxO3a Signal Pathway

    doi: 10.1155/2018/6451902

    Figure Lengend Snippet: ZP2495 exerted antiapoptotic effect on high-glucose-induced NRVMs after SI/R injury. (a) Apoptosis of the NRVMs determined by annexin V/propidium iodide (PI) double staining and flow cytometry. Region Q2 late apoptotic cells, region Q3 vital cells, and region Q4 early apoptotic cells. (b) Representative images of immunostaining for apoptotic (TUNEL) cells (scale bar = 100 μ m). (c) Quantification of apoptosis cell by flow cytometry and TUNEL staining. (d) Quantification of apoptosis cell by TUNEL staining. (e) Activities of caspase-3. Con: normal glucose medium group; HG: high-glucose medium group; HG + SI/R: HG + SI/R group; ZP2495: HG + SI/R + ZP2495 group; glucagon: HG + SI/R + glucagon group; and ZP131: HG + SI/R + ZP131 group. Presented values are mean ± SEM. † P

    Article Snippet: The following primary antibodies were used: anti-phospho-FoxO3a (Thr32) (rabbit polyclonal IgG, Abcam, 1 : 100); anti-phospho-FoxO3a (Ser413) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-FoxO3a (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-Akt (Ser473) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-Akt (Thr308) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-Akt (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-AMPK (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-AMPK (Thr172) (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-caspase-3 (rabbit polyclonal IgG, Merck Millipore, 1 : 200); anti-cleaved caspase-3 (rabbit polyclonal IgG, Merck Millipore, 1 : 200); anti-Bim (rabbit polyclonal IgG, Abcam, 1 : 1000); anti-Bax (rabbit polyclonal IgG, Abcam, 1 : 1000); anti-Bcl-2 (rabbit monoclonal IgG, Abcam, 1 : 500); anti-Bad (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-phospho-Bad (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000); anti-MnSOD (rabbit polyclonal IgG, Abcam, 1 : 5000); anti-Catalase (rabbit polyclonal IgG, Abcam, 1 : 2000); anti-Sirt1 (rabbit polyclonal IgG, Abcam, 1 : 1000); anti-PGC-1α (rabbit polyclonal IgG, Abcam, 1 : 1000); acetylated-lysine (rabbit polyclonal IgG, Cell Signaling Technology, 1 : 1000,); anti-Nrf-1 (rabbit monoclonal IgG, Abcam, 1 : 5000); anti-Tfam (rabbit polyclonal IgG, Abcam, 1 : 2000); and β -actin (rabbit polyclonal IgG, Santa Cruz Biotechnology, 1 : 1000).

    Techniques: Double Staining, Flow Cytometry, Cytometry, Immunostaining, TUNEL Assay, Staining