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Epitomics anti cleaved caspase 3
Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and <t>cleaved-caspase-3</t> ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs
Anti Cleaved Caspase 3, supplied by Epitomics, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cleaved caspase 3/product/Epitomics
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
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90/100 stars

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1) Product Images from "The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivo"

Article Title: The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivo

Journal: Particle and Fibre Toxicology

doi: 10.1186/s12989-016-0163-3

Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and cleaved-caspase-3 ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs
Figure Legend Snippet: Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and cleaved-caspase-3 ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs

Techniques Used: Expressing, Immunohistochemistry, Staining, Western Blot, Mouse Assay, Injection

2) Product Images from "Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture"

Article Title: Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture

Journal: Scientific Reports

doi: 10.1038/s41598-017-10708-0

Early-passage MSCs increase in survival compared with late-passage MSCs on chitosan film. MSCs of early and late passages were seeded in dishes coated with chitosan, followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. *p 
Figure Legend Snippet: Early-passage MSCs increase in survival compared with late-passage MSCs on chitosan film. MSCs of early and late passages were seeded in dishes coated with chitosan, followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. *p 

Techniques Used: Staining, TUNEL Assay, Western Blot, Standard Deviation

Chitosan film culture activates mTOR to activate S6K/S6/4EBP while inactivate Autophagy. ( a ) Cells were seeded at 2.5×10 4 /cm 2  in dishes with chitosan coating for 48 hr, followed by immunostaining using antibodies against c-caspase 3 or LC3A. DAPI merged images are shown in the low panel (Scale bar = 50 μM). ( b ) The spheroid cells treated with compound c or metformin were then assayed for Live/Dead staining (Scale bar = 50 μM). ( c ) A scheme of the proposed model. Chitosan 3D sphere culture of MSCs enhances stem cell properties by selection of more primitive cells, which increase autophagy and thereby survive, while senescent cells decrease autophagy and undergo apoptosis. Especially in senescent cells, chitosan 3D sphere culture activates mTOR, which activates S6K/S6/4EBP1 to enhance fibronection synthesis and peripheral dead cell attachment, and phosphorylates ULK1 at S757 to further inactivate ULK1/Beclin and autophagy, thereby inducing apoptosis. Combination of chitosan 3D sphere culture with mTOR inhibition prevents peripheral dead cell attachment, thereby further increasing pluripotent gene expression,  in vitro  osteogenesis and  in vivo  bone formation.
Figure Legend Snippet: Chitosan film culture activates mTOR to activate S6K/S6/4EBP while inactivate Autophagy. ( a ) Cells were seeded at 2.5×10 4 /cm 2 in dishes with chitosan coating for 48 hr, followed by immunostaining using antibodies against c-caspase 3 or LC3A. DAPI merged images are shown in the low panel (Scale bar = 50 μM). ( b ) The spheroid cells treated with compound c or metformin were then assayed for Live/Dead staining (Scale bar = 50 μM). ( c ) A scheme of the proposed model. Chitosan 3D sphere culture of MSCs enhances stem cell properties by selection of more primitive cells, which increase autophagy and thereby survive, while senescent cells decrease autophagy and undergo apoptosis. Especially in senescent cells, chitosan 3D sphere culture activates mTOR, which activates S6K/S6/4EBP1 to enhance fibronection synthesis and peripheral dead cell attachment, and phosphorylates ULK1 at S757 to further inactivate ULK1/Beclin and autophagy, thereby inducing apoptosis. Combination of chitosan 3D sphere culture with mTOR inhibition prevents peripheral dead cell attachment, thereby further increasing pluripotent gene expression, in vitro osteogenesis and in vivo bone formation.

Techniques Used: Immunostaining, Staining, Selection, Cell Attachment Assay, Inhibition, Expressing, In Vitro, In Vivo

The spheroid formation on chitosan film is associated with an increase in Apoptosis. MSCs were seeded at 2.5 × 10 4 /cm 2  without (Monolayer) or with chitosan coating (Spheroid), followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. ***p 
Figure Legend Snippet: The spheroid formation on chitosan film is associated with an increase in Apoptosis. MSCs were seeded at 2.5 × 10 4 /cm 2 without (Monolayer) or with chitosan coating (Spheroid), followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. ***p 

Techniques Used: Staining, TUNEL Assay, Western Blot, Standard Deviation

3) Product Images from "MicroRNA-200a confers chemoresistance by antagonizing TP53INP1 and YAP1 in human breast cancer"

Article Title: MicroRNA-200a confers chemoresistance by antagonizing TP53INP1 and YAP1 in human breast cancer

Journal: BMC Cancer

doi: 10.1186/s12885-017-3930-0

miR-200a promoted chemoresistance in breast cancer cell lines. a MDA-MB-231 cells that had been transfected with the miR-200a mimic or the miR-Ctrl were treated with 5 μM cis-platin and stained with TUNEL-TMR red, phalloidin-FITC for actin and DAPI for the cell nucleus. b The confocal TUNEL analysis showed that the miR-200a-transfected MDA-MB-231 cells had lower levels of apoptosis than the miR-Ctrl-transfected MDA-MB-231 cells. c Apoptosis was evaluated in ZR-75-30 cells after treating with cis-platin and staining with Annexin-V at 48 h. The flow cytometry profile depicts Annexin-V-FITC staining on the x -axis and PI staining on the y -axis. The number represents the percentage of early apoptotic cells in each condition, and ( d ) Mean ± SEM of apoptotic cells from three different experiments. e After cis-platin treatment, the transfected cells were lysed for western blotting. The protein levels of cleaved caspase-3 were normalized to GAPDH
Figure Legend Snippet: miR-200a promoted chemoresistance in breast cancer cell lines. a MDA-MB-231 cells that had been transfected with the miR-200a mimic or the miR-Ctrl were treated with 5 μM cis-platin and stained with TUNEL-TMR red, phalloidin-FITC for actin and DAPI for the cell nucleus. b The confocal TUNEL analysis showed that the miR-200a-transfected MDA-MB-231 cells had lower levels of apoptosis than the miR-Ctrl-transfected MDA-MB-231 cells. c Apoptosis was evaluated in ZR-75-30 cells after treating with cis-platin and staining with Annexin-V at 48 h. The flow cytometry profile depicts Annexin-V-FITC staining on the x -axis and PI staining on the y -axis. The number represents the percentage of early apoptotic cells in each condition, and ( d ) Mean ± SEM of apoptotic cells from three different experiments. e After cis-platin treatment, the transfected cells were lysed for western blotting. The protein levels of cleaved caspase-3 were normalized to GAPDH

Techniques Used: Multiple Displacement Amplification, Transfection, Staining, TUNEL Assay, Flow Cytometry, Cytometry, Western Blot

4) Product Images from "The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivo"

Article Title: The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivo

Journal: Particle and Fibre Toxicology

doi: 10.1186/s12989-016-0163-3

Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and cleaved-caspase-3 ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs
Figure Legend Snippet: Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and cleaved-caspase-3 ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs

Techniques Used: Expressing, Immunohistochemistry, Staining, Western Blot, Mouse Assay, Injection

5) Product Images from "Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture"

Article Title: Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture

Journal: Scientific Reports

doi: 10.1038/s41598-017-10708-0

Early-passage MSCs increase in survival compared with late-passage MSCs on chitosan film. MSCs of early and late passages were seeded in dishes coated with chitosan, followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. *p 
Figure Legend Snippet: Early-passage MSCs increase in survival compared with late-passage MSCs on chitosan film. MSCs of early and late passages were seeded in dishes coated with chitosan, followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. *p 

Techniques Used: Staining, TUNEL Assay, Western Blot, Standard Deviation

Chitosan film culture activates mTOR to activate S6K/S6/4EBP while inactivate Autophagy. ( a ) Cells were seeded at 2.5×10 4 /cm 2  in dishes with chitosan coating for 48 hr, followed by immunostaining using antibodies against c-caspase 3 or LC3A. DAPI merged images are shown in the low panel (Scale bar = 50 μM). ( b ) The spheroid cells treated with compound c or metformin were then assayed for Live/Dead staining (Scale bar = 50 μM). ( c ) A scheme of the proposed model. Chitosan 3D sphere culture of MSCs enhances stem cell properties by selection of more primitive cells, which increase autophagy and thereby survive, while senescent cells decrease autophagy and undergo apoptosis. Especially in senescent cells, chitosan 3D sphere culture activates mTOR, which activates S6K/S6/4EBP1 to enhance fibronection synthesis and peripheral dead cell attachment, and phosphorylates ULK1 at S757 to further inactivate ULK1/Beclin and autophagy, thereby inducing apoptosis. Combination of chitosan 3D sphere culture with mTOR inhibition prevents peripheral dead cell attachment, thereby further increasing pluripotent gene expression,  in vitro  osteogenesis and  in vivo  bone formation.
Figure Legend Snippet: Chitosan film culture activates mTOR to activate S6K/S6/4EBP while inactivate Autophagy. ( a ) Cells were seeded at 2.5×10 4 /cm 2 in dishes with chitosan coating for 48 hr, followed by immunostaining using antibodies against c-caspase 3 or LC3A. DAPI merged images are shown in the low panel (Scale bar = 50 μM). ( b ) The spheroid cells treated with compound c or metformin were then assayed for Live/Dead staining (Scale bar = 50 μM). ( c ) A scheme of the proposed model. Chitosan 3D sphere culture of MSCs enhances stem cell properties by selection of more primitive cells, which increase autophagy and thereby survive, while senescent cells decrease autophagy and undergo apoptosis. Especially in senescent cells, chitosan 3D sphere culture activates mTOR, which activates S6K/S6/4EBP1 to enhance fibronection synthesis and peripheral dead cell attachment, and phosphorylates ULK1 at S757 to further inactivate ULK1/Beclin and autophagy, thereby inducing apoptosis. Combination of chitosan 3D sphere culture with mTOR inhibition prevents peripheral dead cell attachment, thereby further increasing pluripotent gene expression, in vitro osteogenesis and in vivo bone formation.

Techniques Used: Immunostaining, Staining, Selection, Cell Attachment Assay, Inhibition, Expressing, In Vitro, In Vivo

The spheroid formation on chitosan film is associated with an increase in Apoptosis. MSCs were seeded at 2.5 × 10 4 /cm 2  without (Monolayer) or with chitosan coating (Spheroid), followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. ***p 
Figure Legend Snippet: The spheroid formation on chitosan film is associated with an increase in Apoptosis. MSCs were seeded at 2.5 × 10 4 /cm 2 without (Monolayer) or with chitosan coating (Spheroid), followed by calculation of cell numbers ( a ), Live/Dead staining (48 hr; Scale bar = 50 μM) ( b ), TUNEL assay (Scale bar = 10 μM) ( c ), and Annexin V/PI assay ( d ) at indicated time points. ( e ) Whole-cell lysates at 48 hr were analyzed by western blotting with specific antibodies against c-caspase-9 and c-caspase 3. β-tubulin was used as a loading control. The results are expressed as the mean ± standard deviation of three independent experiments, which is representative of MSCs from two individuals. ***p 

Techniques Used: Staining, TUNEL Assay, Western Blot, Standard Deviation

6) Product Images from "MicroRNA-200a confers chemoresistance by antagonizing TP53INP1 and YAP1 in human breast cancer"

Article Title: MicroRNA-200a confers chemoresistance by antagonizing TP53INP1 and YAP1 in human breast cancer

Journal: BMC Cancer

doi: 10.1186/s12885-017-3930-0

miR-200a promoted chemoresistance in breast cancer cell lines. a MDA-MB-231 cells that had been transfected with the miR-200a mimic or the miR-Ctrl were treated with 5 μM cis-platin and stained with TUNEL-TMR red, phalloidin-FITC for actin and DAPI for the cell nucleus. b The confocal TUNEL analysis showed that the miR-200a-transfected MDA-MB-231 cells had lower levels of apoptosis than the miR-Ctrl-transfected MDA-MB-231 cells. c Apoptosis was evaluated in ZR-75-30 cells after treating with cis-platin and staining with Annexin-V at 48 h. The flow cytometry profile depicts Annexin-V-FITC staining on the x -axis and PI staining on the y -axis. The number represents the percentage of early apoptotic cells in each condition, and ( d ) Mean ± SEM of apoptotic cells from three different experiments. e After cis-platin treatment, the transfected cells were lysed for western blotting. The protein levels of cleaved caspase-3 were normalized to GAPDH
Figure Legend Snippet: miR-200a promoted chemoresistance in breast cancer cell lines. a MDA-MB-231 cells that had been transfected with the miR-200a mimic or the miR-Ctrl were treated with 5 μM cis-platin and stained with TUNEL-TMR red, phalloidin-FITC for actin and DAPI for the cell nucleus. b The confocal TUNEL analysis showed that the miR-200a-transfected MDA-MB-231 cells had lower levels of apoptosis than the miR-Ctrl-transfected MDA-MB-231 cells. c Apoptosis was evaluated in ZR-75-30 cells after treating with cis-platin and staining with Annexin-V at 48 h. The flow cytometry profile depicts Annexin-V-FITC staining on the x -axis and PI staining on the y -axis. The number represents the percentage of early apoptotic cells in each condition, and ( d ) Mean ± SEM of apoptotic cells from three different experiments. e After cis-platin treatment, the transfected cells were lysed for western blotting. The protein levels of cleaved caspase-3 were normalized to GAPDH

Techniques Used: Multiple Displacement Amplification, Transfection, Staining, TUNEL Assay, Flow Cytometry, Cytometry, Western Blot

Related Articles

Incubation:

Article Title: Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture
Article Snippet: .. The following primary antibodies were incubated overnight at 4 °C: anti-phospho-mTOR (Ser2448) (1:200, Bioworld), anti-cleaved caspase-3 (1:200, Epitomics), anti-fibronectin (1:200, Santa Cruz) anti-phospho-ULK1 (ser757) (1:1000, Cell Signaling), anti-phospho-S6 (Ser235/236) (1:200, Cell Signaling) or anti-LC3A (1:50, Cell Signaling). .. After incubation with primary antibodies, cells were washed with PBS and then incubated with fluorescence-conjugated secondary antibodies (1:5000, FITC, Jackson ImmunoResearch, Westgrove, PA) for 2 hr at room temperature.

Article Title: The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivo
Article Snippet: .. Tissue sections were immunohistochemically incubated overnight at 4 °C with the anti-LC3 (MBL, Japan), anti-HIF-1α (BD Transduction Laboratories, USA) or anti-cleaved-caspase 3 (Epitomics, USA) antibodies. .. The sections were then incubated with a secondary antibody for 1 h at room temperature, and the slides were developed using the STARR TREK Universal HRP detection kit (Biocare Medical, Concord, CA).

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    Epitomics anti pro caspase 3
    Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and <t>cleaved-caspase-3</t> ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs
    Anti Pro Caspase 3, supplied by Epitomics, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pro caspase 3/product/Epitomics
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti pro caspase 3 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    89
    Epitomics rabbit anti human caspase 3 antibody
    Immunohistochemical staining. (A) Expression of NIS, <t>caspase-3</t> and Ki-67 in CNE-2Z and CNE-2Z-NIS xenograft tumors treated with 131 I or PBS (magnification, 200×). (B) Immunohistochemical analysis of protein NIS, caspase3 and Ki67. High expression of NIS protein was observed in the cells of the CNE-2Z-NIS xenografts with 131 I or PBS treatment compared with the cells of the CNE-2Z xenografts with 131 I or PBS treatment ( p
    Rabbit Anti Human Caspase 3 Antibody, supplied by Epitomics, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human caspase 3 antibody/product/Epitomics
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human caspase 3 antibody - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    91
    Epitomics rabbit anti activated caspase 3
    Mito-hTERT reduces cell apoptosis induced by chemotherapeutic drugs (A) Activited <t>caspase-3</t> in lentivirus-transduced SK-Hep1 and HepG2 cells was detected by immunofluorescence assay after treated with 0.5 mg/ml DOX, 5 mg/ml CDDP or 10 mg/ml 5-FU for 12
    Rabbit Anti Activated Caspase 3, supplied by Epitomics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti activated caspase 3/product/Epitomics
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti activated caspase 3 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Epitomics rabbit monoclonal anti human pro caspase 3
    The effect of Pam3CSK4 and Pam3CSK4+Velcade on cleavage of <t>pro-caspase-3</t> protein in the presence or absence of FN in L363 ( a ), OPM-2 ( b ) and U266 ( c ) HMCLs. After treatment with Pam3CSK4 with or without Velcade, non-adhered and FN-adhered HMCLs were analyzed for cleaved and pro-enzyme forms of caspase-3 using western blotting as described in Materials and Methods. β-Actin was used as a housekeeping protein.
    Rabbit Monoclonal Anti Human Pro Caspase 3, supplied by Epitomics, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti human pro caspase 3/product/Epitomics
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti human pro caspase 3 - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and cleaved-caspase-3 ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs

    Journal: Particle and Fibre Toxicology

    Article Title: The role of hypoxia-inducible factor-1α in zinc oxide nanoparticle-induced nephrotoxicity in vitro and in vivo

    doi: 10.1186/s12989-016-0163-3

    Figure Lengend Snippet: Protein expression in the kidney tissues using IHC staining and western blot analysis. IHC was used to determine the expression levels of HIF-1α ( a ), LC3 ( b ) and cleaved-caspase-3 ( c ). d Western blot analysis of protein expression in kidney tissues. The mice were i.p. injected with ZnO NPs

    Article Snippet: Anti-PAI-1, anti-Bax and anti-Beclin 1 were obtained from Cell Signaling Technology (Ipswich, MA, USA); anti-HIF-1α was obtained from BD Transduction Laboratories (San Diego, CA, USA); anti-GAPDH was obtained from Abcam (Cambridge, MA, USA); anti-LC3 and anti-CTGF were obtained from Abgent (San Diego, CA, USA); anti-p62/SQSTM1 was obtained from MBL (Nagoya, Japan); anti-pro-caspase 3 and anti-cleaved-caspase 3 were obtained from Epitomics (Burlingame, CA, USA).

    Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, Mouse Assay, Injection

    Immunohistochemical staining. (A) Expression of NIS, caspase-3 and Ki-67 in CNE-2Z and CNE-2Z-NIS xenograft tumors treated with 131 I or PBS (magnification, 200×). (B) Immunohistochemical analysis of protein NIS, caspase3 and Ki67. High expression of NIS protein was observed in the cells of the CNE-2Z-NIS xenografts with 131 I or PBS treatment compared with the cells of the CNE-2Z xenografts with 131 I or PBS treatment ( p

    Journal: PLoS ONE

    Article Title: In vivo Molecular Imaging and Radionuclide (131I) Therapy of Human Nasopharyngeal Carcinoma Cells Transfected with a Lentivirus Expressing Sodium Iodide Symporter

    doi: 10.1371/journal.pone.0116531

    Figure Lengend Snippet: Immunohistochemical staining. (A) Expression of NIS, caspase-3 and Ki-67 in CNE-2Z and CNE-2Z-NIS xenograft tumors treated with 131 I or PBS (magnification, 200×). (B) Immunohistochemical analysis of protein NIS, caspase3 and Ki67. High expression of NIS protein was observed in the cells of the CNE-2Z-NIS xenografts with 131 I or PBS treatment compared with the cells of the CNE-2Z xenografts with 131 I or PBS treatment ( p

    Article Snippet: Histology and immunohistochemistry 25 days after administration of 131 I, the animals were sacrificed by cervical vertebra dislocation and the tumors were, cryosectioned (5 μm) and subjected to immunohistochemical analysis using rabbit anti-human NIS antibody (1:50; Proteintech, CHI, USA), rabbit anti-human caspase-3 antibody (1:30; Epitomics, California, USA) and rabbit anti-human Ki67 antibody (1:200; Thermo Scientific, Fremont, USA).

    Techniques: Immunohistochemistry, Staining, Expressing

    Mito-hTERT reduces cell apoptosis induced by chemotherapeutic drugs (A) Activited caspase-3 in lentivirus-transduced SK-Hep1 and HepG2 cells was detected by immunofluorescence assay after treated with 0.5 mg/ml DOX, 5 mg/ml CDDP or 10 mg/ml 5-FU for 12

    Journal: American Journal of Translational Research

    Article Title: Impact of mitochondrial telomerase over-expression on drug resistance of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: Mito-hTERT reduces cell apoptosis induced by chemotherapeutic drugs (A) Activited caspase-3 in lentivirus-transduced SK-Hep1 and HepG2 cells was detected by immunofluorescence assay after treated with 0.5 mg/ml DOX, 5 mg/ml CDDP or 10 mg/ml 5-FU for 12

    Article Snippet: Briefly, cells were washed thrice with PBS and incubated with 300-500 µl of 400 nM MitoTracker Deep Red (Molecular probes, Invitrogen, Life Technologies) at 37°C for 30 min. Then, cells were fixed in 4% paraformaldehyde for 15 min at room temperature, permeabilized with 0.3% Triton X-100 (Amresco LLC., Solon, OH) for 10 min at 4°C and blocked with 2 mg/ml of BSA (Amresco LLC., Solon, OH) for 1 h at 37°C, followed by incubation with an rabbit anti-hTERT monoclonal antibody (1:50, Abcam, Cambridge, MA) and rabbit anti-activated caspase-3 (1:10, Epitomics, Burlingame, CA) for 12 h at 4°C.

    Techniques: Immunofluorescence

    The effect of Pam3CSK4 and Pam3CSK4+Velcade on cleavage of pro-caspase-3 protein in the presence or absence of FN in L363 ( a ), OPM-2 ( b ) and U266 ( c ) HMCLs. After treatment with Pam3CSK4 with or without Velcade, non-adhered and FN-adhered HMCLs were analyzed for cleaved and pro-enzyme forms of caspase-3 using western blotting as described in Materials and Methods. β-Actin was used as a housekeeping protein.

    Journal: Blood Cancer Journal

    Article Title: Stimulation of Toll-like receptor-1/2 combined with Velcade increases cytotoxicity to human multiple myeloma cells

    doi: 10.1038/bcj.2013.17

    Figure Lengend Snippet: The effect of Pam3CSK4 and Pam3CSK4+Velcade on cleavage of pro-caspase-3 protein in the presence or absence of FN in L363 ( a ), OPM-2 ( b ) and U266 ( c ) HMCLs. After treatment with Pam3CSK4 with or without Velcade, non-adhered and FN-adhered HMCLs were analyzed for cleaved and pro-enzyme forms of caspase-3 using western blotting as described in Materials and Methods. β-Actin was used as a housekeeping protein.

    Article Snippet: The antibodies used in western blotting experiments were as follows: rabbit monoclonal, anti-human cleaved caspase-3 (clone D3E9, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-human pro-caspase-3 (clone E83–103, Epitomics, Burlingame, CA, USA), rabbit polyclonal anti-human p53, p73, BCL-2, Bax (GeneTex, Irvine, CA, USA), HRP-conjugated goat anti-rabbit immunoglobulins from DAKO (Glostrup, Denmark), or anti-rabbit IgG and IgG1 from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Western Blot

    The effect of Pam3CSK4 and Pam3CSK4+Velcade on caspase-3 enzymatic activity in the presence or absence of FN in L363 ( a ), OPM-2 ( b ) and U266 ( c ) HMCLs. Caspase-3 activity was determined by the release of the fluorescent 7-amino-4-methylcoumarin (AMC) moiety following hydrolysis of the peptide substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC) by the activated enzyme. Data represent calculated mean±s.e.m. of two separate experiments. * P

    Journal: Blood Cancer Journal

    Article Title: Stimulation of Toll-like receptor-1/2 combined with Velcade increases cytotoxicity to human multiple myeloma cells

    doi: 10.1038/bcj.2013.17

    Figure Lengend Snippet: The effect of Pam3CSK4 and Pam3CSK4+Velcade on caspase-3 enzymatic activity in the presence or absence of FN in L363 ( a ), OPM-2 ( b ) and U266 ( c ) HMCLs. Caspase-3 activity was determined by the release of the fluorescent 7-amino-4-methylcoumarin (AMC) moiety following hydrolysis of the peptide substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC) by the activated enzyme. Data represent calculated mean±s.e.m. of two separate experiments. * P

    Article Snippet: The antibodies used in western blotting experiments were as follows: rabbit monoclonal, anti-human cleaved caspase-3 (clone D3E9, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-human pro-caspase-3 (clone E83–103, Epitomics, Burlingame, CA, USA), rabbit polyclonal anti-human p53, p73, BCL-2, Bax (GeneTex, Irvine, CA, USA), HRP-conjugated goat anti-rabbit immunoglobulins from DAKO (Glostrup, Denmark), or anti-rabbit IgG and IgG1 from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Activity Assay