Structured Review

Biocare Medical anti cleaved caspase 3
TLR4 deficiency results in decreased neuronal apoptosis in the HF in response to GCI/R Neuronal apoptosis was confirmed by the methods of TUNEL staining (A to F) and IHC staining for cleaved <t>caspase-3,</t> a specific marker of apoptosis (G to L). The positive staining was shown in brown. Slides were counterstained with hematoxylin. Results show that GCI/R results in increased numbers of TUNEL positive cells in the HF (B and E) in WT mice. In TLR4 -/- -I/R mice, there were fewer TUNEL positive staining cells in the HF (C and F) compared with WT-I/R mice (M, # p
Anti Cleaved Caspase 3, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cleaved caspase 3/product/Biocare Medical
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
anti cleaved caspase 3 - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Activation of Toll-like Receptor 4 Signaling Contributes to Hippocampal Neuronal Death Following Global Cerebral Ischemia/Reperfusion"

Article Title: Activation of Toll-like Receptor 4 Signaling Contributes to Hippocampal Neuronal Death Following Global Cerebral Ischemia/Reperfusion

Journal: Journal of neuroimmunology

doi: 10.1016/j.jneuroim.2007.08.014

TLR4 deficiency results in decreased neuronal apoptosis in the HF in response to GCI/R Neuronal apoptosis was confirmed by the methods of TUNEL staining (A to F) and IHC staining for cleaved caspase-3, a specific marker of apoptosis (G to L). The positive staining was shown in brown. Slides were counterstained with hematoxylin. Results show that GCI/R results in increased numbers of TUNEL positive cells in the HF (B and E) in WT mice. In TLR4 -/- -I/R mice, there were fewer TUNEL positive staining cells in the HF (C and F) compared with WT-I/R mice (M, # p
Figure Legend Snippet: TLR4 deficiency results in decreased neuronal apoptosis in the HF in response to GCI/R Neuronal apoptosis was confirmed by the methods of TUNEL staining (A to F) and IHC staining for cleaved caspase-3, a specific marker of apoptosis (G to L). The positive staining was shown in brown. Slides were counterstained with hematoxylin. Results show that GCI/R results in increased numbers of TUNEL positive cells in the HF (B and E) in WT mice. In TLR4 -/- -I/R mice, there were fewer TUNEL positive staining cells in the HF (C and F) compared with WT-I/R mice (M, # p

Techniques Used: TUNEL Assay, Staining, Immunohistochemistry, Marker, Mouse Assay

2) Product Images from "Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice"

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

doi: 10.1152/ajplung.00560.2017

Deficits in proliferation and survival of the cells of saccular and alveolar lungs exposed to a single dose of LPS. Newborn mice were injected intraperitoneally with a single dose of vehicle control (PBS) or 10 mg/kg LPS (L10) on postnatal day (PND)3 or PND7, and the lung tissues were harvested on PND14 for lung immunohistochemistry studies. Representative lung sections showing Ki67-stained cells from mice treated on PND3 with PBS ( A ) and L10 ( B ) and on PND7 with PBS ( C ) and L10 ( D ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated on PND3 with PBS ( E ) and L10 ( F ) and on PND7 with PBS ( G ) and L10 ( H ). Quantification of Ki67-positive (percentage) ( I ) and cleaved caspase 3-positive (number per high power field) ( J ) lung cells. Values are presented as means ± SD ( n  = 6/group). Significant differences between age-matched PBS- and L10-exposed animals are indicated by *** P
Figure Legend Snippet: Deficits in proliferation and survival of the cells of saccular and alveolar lungs exposed to a single dose of LPS. Newborn mice were injected intraperitoneally with a single dose of vehicle control (PBS) or 10 mg/kg LPS (L10) on postnatal day (PND)3 or PND7, and the lung tissues were harvested on PND14 for lung immunohistochemistry studies. Representative lung sections showing Ki67-stained cells from mice treated on PND3 with PBS ( A ) and L10 ( B ) and on PND7 with PBS ( C ) and L10 ( D ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated on PND3 with PBS ( E ) and L10 ( F ) and on PND7 with PBS ( G ) and L10 ( H ). Quantification of Ki67-positive (percentage) ( I ) and cleaved caspase 3-positive (number per high power field) ( J ) lung cells. Values are presented as means ± SD ( n = 6/group). Significant differences between age-matched PBS- and L10-exposed animals are indicated by *** P

Techniques Used: Mouse Assay, Injection, Immunohistochemistry, Staining

Effect of chronic LPS exposure on lung cell proliferation and apoptosis. Newborn mice were treated intraperitoneally with 10 mg/kg of LPS (L10) or a vehicle control (PBS) on  postnatal days  (PNDs)  3–5 , and the lung tissues were harvested for immunohistochemistry studies on PND7. Representative lung sections showing Ki67-stained cells from mice treated with PBS ( A ) and L10 ( B ). Quantification of the percentage of Ki67-positive lung cells ( C ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated with PBS ( D ) and L10 ( E ). Quantification of cleaved caspase 3-positive lung cells ( F ). HPF, high-power field. Values are presented as means ± SD ( n  = 6/group). Significant differences between PBS- and L10-treated animals are indicated by *** P
Figure Legend Snippet: Effect of chronic LPS exposure on lung cell proliferation and apoptosis. Newborn mice were treated intraperitoneally with 10 mg/kg of LPS (L10) or a vehicle control (PBS) on postnatal days (PNDs) 3–5 , and the lung tissues were harvested for immunohistochemistry studies on PND7. Representative lung sections showing Ki67-stained cells from mice treated with PBS ( A ) and L10 ( B ). Quantification of the percentage of Ki67-positive lung cells ( C ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated with PBS ( D ) and L10 ( E ). Quantification of cleaved caspase 3-positive lung cells ( F ). HPF, high-power field. Values are presented as means ± SD ( n = 6/group). Significant differences between PBS- and L10-treated animals are indicated by *** P

Techniques Used: Mouse Assay, Immunohistochemistry, Staining

3) Product Images from "Human Plasma-Derived 3D Cultures Model Breast Cancer Treatment Responses and Predict Clinically Effective Drug Treatment Concentrations"

Article Title: Human Plasma-Derived 3D Cultures Model Breast Cancer Treatment Responses and Predict Clinically Effective Drug Treatment Concentrations

Journal: Cancers

doi: 10.3390/cancers12071722

HuP3D cultures allow BCa cell proliferation. ( a ) HuP3D matrices are created through the cross-linking of plasma fibrinogen and the matrices can include pre-labeled BCa cells from 5 cell lines representing different BCa subtypes in culture with numerous variations of microenvironment components. These matrices were cultured for 0.5, 3, and 7 days followed by enzymatic digestion and single cell analysis using flow cytometry, immunohistochemistry (IHC), or confocal imaging. ( b ) Cell proliferation and apoptosis of the five BCa cell lines either alone, in co-culture with a healthy microenvironment (HME), or in co-culture with a tumor microenvironment (TME) in the HuP3D matrix presented as ( i ) cell fold of γ 0 for 3 and 7 days (mean ± SD, n = 4); ( ii ) representative flow cytometry histograms of FITC-pAKT and V450-cleaved caspase 3 signals compared to the fluorescence minus one (FMO) control at day 7 for the MDA-MB-231 BCa cell line (mean ± SD, n = 3); ( iii ) representative IHC images of the MDA-MB-231 cell line for Ki67 and caspase 3 staining at days 3 and 7, scale bar = 600 µm; ( iv ) representative confocal images on day 3 and day 7 to monitor proliferation of the MDA-MB-231 BCa cell line grown within the HuP3D (DiO: green). Scale bar = 1000 µm. ** p
Figure Legend Snippet: HuP3D cultures allow BCa cell proliferation. ( a ) HuP3D matrices are created through the cross-linking of plasma fibrinogen and the matrices can include pre-labeled BCa cells from 5 cell lines representing different BCa subtypes in culture with numerous variations of microenvironment components. These matrices were cultured for 0.5, 3, and 7 days followed by enzymatic digestion and single cell analysis using flow cytometry, immunohistochemistry (IHC), or confocal imaging. ( b ) Cell proliferation and apoptosis of the five BCa cell lines either alone, in co-culture with a healthy microenvironment (HME), or in co-culture with a tumor microenvironment (TME) in the HuP3D matrix presented as ( i ) cell fold of γ 0 for 3 and 7 days (mean ± SD, n = 4); ( ii ) representative flow cytometry histograms of FITC-pAKT and V450-cleaved caspase 3 signals compared to the fluorescence minus one (FMO) control at day 7 for the MDA-MB-231 BCa cell line (mean ± SD, n = 3); ( iii ) representative IHC images of the MDA-MB-231 cell line for Ki67 and caspase 3 staining at days 3 and 7, scale bar = 600 µm; ( iv ) representative confocal images on day 3 and day 7 to monitor proliferation of the MDA-MB-231 BCa cell line grown within the HuP3D (DiO: green). Scale bar = 1000 µm. ** p

Techniques Used: Labeling, Cell Culture, Single-cell Analysis, Flow Cytometry, Immunohistochemistry, Imaging, Co-Culture Assay, Fluorescence, Multiple Displacement Amplification, Staining

4) Product Images from "Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice"

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

doi: 10.1152/ajplung.00560.2017

Deficits in proliferation and survival of the cells of saccular and alveolar lungs exposed to a single dose of LPS. Newborn mice were injected intraperitoneally with a single dose of vehicle control (PBS) or 10 mg/kg LPS (L10) on postnatal day (PND)3 or PND7, and the lung tissues were harvested on PND14 for lung immunohistochemistry studies. Representative lung sections showing Ki67-stained cells from mice treated on PND3 with PBS ( A ) and L10 ( B ) and on PND7 with PBS ( C ) and L10 ( D ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated on PND3 with PBS ( E ) and L10 ( F ) and on PND7 with PBS ( G ) and L10 ( H ). Quantification of Ki67-positive (percentage) ( I ) and cleaved caspase 3-positive (number per high power field) ( J ) lung cells. Values are presented as means ± SD ( n  = 6/group). Significant differences between age-matched PBS- and L10-exposed animals are indicated by *** P
Figure Legend Snippet: Deficits in proliferation and survival of the cells of saccular and alveolar lungs exposed to a single dose of LPS. Newborn mice were injected intraperitoneally with a single dose of vehicle control (PBS) or 10 mg/kg LPS (L10) on postnatal day (PND)3 or PND7, and the lung tissues were harvested on PND14 for lung immunohistochemistry studies. Representative lung sections showing Ki67-stained cells from mice treated on PND3 with PBS ( A ) and L10 ( B ) and on PND7 with PBS ( C ) and L10 ( D ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated on PND3 with PBS ( E ) and L10 ( F ) and on PND7 with PBS ( G ) and L10 ( H ). Quantification of Ki67-positive (percentage) ( I ) and cleaved caspase 3-positive (number per high power field) ( J ) lung cells. Values are presented as means ± SD ( n = 6/group). Significant differences between age-matched PBS- and L10-exposed animals are indicated by *** P

Techniques Used: Mouse Assay, Injection, Immunohistochemistry, Staining

Effect of chronic LPS exposure on lung cell proliferation and apoptosis. Newborn mice were treated intraperitoneally with 10 mg/kg of LPS (L10) or a vehicle control (PBS) on  postnatal days  (PNDs)  3–5 , and the lung tissues were harvested for immunohistochemistry studies on PND7. Representative lung sections showing Ki67-stained cells from mice treated with PBS ( A ) and L10 ( B ). Quantification of the percentage of Ki67-positive lung cells ( C ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated with PBS ( D ) and L10 ( E ). Quantification of cleaved caspase 3-positive lung cells ( F ). HPF, high-power field. Values are presented as means ± SD ( n  = 6/group). Significant differences between PBS- and L10-treated animals are indicated by *** P
Figure Legend Snippet: Effect of chronic LPS exposure on lung cell proliferation and apoptosis. Newborn mice were treated intraperitoneally with 10 mg/kg of LPS (L10) or a vehicle control (PBS) on postnatal days (PNDs) 3–5 , and the lung tissues were harvested for immunohistochemistry studies on PND7. Representative lung sections showing Ki67-stained cells from mice treated with PBS ( A ) and L10 ( B ). Quantification of the percentage of Ki67-positive lung cells ( C ). Representative lung sections showing cleaved caspase 3-positive cells from mice treated with PBS ( D ) and L10 ( E ). Quantification of cleaved caspase 3-positive lung cells ( F ). HPF, high-power field. Values are presented as means ± SD ( n = 6/group). Significant differences between PBS- and L10-treated animals are indicated by *** P

Techniques Used: Mouse Assay, Immunohistochemistry, Staining

Related Articles

Protein Binding:

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
Article Snippet: .. The lung sections were then incubated with 7.5% normal donkey serum for 1 h to block nonspecific protein binding, after which they were incubated overnight at 4°C with the following primary antibodies: anti-vWF (Dako, Carpinteria, CA; Cat. No. A0082, dilution 1:750), anti-phospho-signal transducer and activator of transcription (STAT)3 (Cell Signaling, Danvers, MA; Cat. No. 9145, dilution 1:150), anti-fibroblast growth factor receptor 1 (FGFR1) (Abcam; Cat. No. ab63601, dilution 1:150), anti-cleaved caspase 3 (Biocare Medical, Pacheco, CA; Cat. No. PP 229 AA, prediluted), and anti-Ki67 (Abcam; Cat. No. ab15580, dilution 1:1,000). .. The Ki67 antibody was detected by incubation with biotinylated anti-rabbit secondary (Vector, Burlingame, CA, dilution 1:200), followed by horseradish peroxidase (Vector, Cat. No. PK-6100).

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
Article Snippet: .. The lung sections were then incubated with 7.5% normal donkey serum for 1 h to block nonspecific protein binding, after which they were incubated overnight at 4°C with the following primary antibodies: anti-vWF (Dako, Carpinteria, CA; Cat. No. A0082, dilution 1:750), anti-phospho-signal transducer and activator of transcription (STAT)3 (Cell Signaling, Danvers, MA; Cat. No. 9145, dilution 1:150), anti-fibroblast growth factor receptor 1 (FGFR1) (Abcam; Cat. No. ab63601, dilution 1:150), anti-cleaved caspase 3 (Biocare Medical, Pacheco, CA; Cat. No. PP 229 AA, prediluted), and anti-Ki67 (Abcam; Cat. No. ab15580, dilution 1:1,000). .. The Ki67 antibody was detected by incubation with biotinylated anti-rabbit secondary (Vector, Burlingame, CA, dilution 1:200), followed by horseradish peroxidase (Vector, Cat. No. PK-6100).

Incubation:

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
Article Snippet: .. The lung sections were then incubated with 7.5% normal donkey serum for 1 h to block nonspecific protein binding, after which they were incubated overnight at 4°C with the following primary antibodies: anti-vWF (Dako, Carpinteria, CA; Cat. No. A0082, dilution 1:750), anti-phospho-signal transducer and activator of transcription (STAT)3 (Cell Signaling, Danvers, MA; Cat. No. 9145, dilution 1:150), anti-fibroblast growth factor receptor 1 (FGFR1) (Abcam; Cat. No. ab63601, dilution 1:150), anti-cleaved caspase 3 (Biocare Medical, Pacheco, CA; Cat. No. PP 229 AA, prediluted), and anti-Ki67 (Abcam; Cat. No. ab15580, dilution 1:1,000). .. The Ki67 antibody was detected by incubation with biotinylated anti-rabbit secondary (Vector, Burlingame, CA, dilution 1:200), followed by horseradish peroxidase (Vector, Cat. No. PK-6100).

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
Article Snippet: .. The lung sections were then incubated with 7.5% normal donkey serum for 1 h to block nonspecific protein binding, after which they were incubated overnight at 4°C with the following primary antibodies: anti-vWF (Dako, Carpinteria, CA; Cat. No. A0082, dilution 1:750), anti-phospho-signal transducer and activator of transcription (STAT)3 (Cell Signaling, Danvers, MA; Cat. No. 9145, dilution 1:150), anti-fibroblast growth factor receptor 1 (FGFR1) (Abcam; Cat. No. ab63601, dilution 1:150), anti-cleaved caspase 3 (Biocare Medical, Pacheco, CA; Cat. No. PP 229 AA, prediluted), and anti-Ki67 (Abcam; Cat. No. ab15580, dilution 1:1,000). .. The Ki67 antibody was detected by incubation with biotinylated anti-rabbit secondary (Vector, Burlingame, CA, dilution 1:200), followed by horseradish peroxidase (Vector, Cat. No. PK-6100).

Immunohistochemistry:

Article Title: Deletion of MCL-1 causes lethal cardiac failure and mitochondrial dysfunction
Article Snippet: .. Paraffin sections were deparaffinized, and immunohistochemistry for detection of Capase-3 and Caspase-7 was performed using the following antibodies: anti-cleaved Caspase-3 and Caspase-7 (BioCare Medical). .. Anti-rabbit on rodent polymer (BioCare Medical) with deaminobenzidine detection system was used for visualization of the antigen.

Blocking Assay:

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
Article Snippet: .. The lung sections were then incubated with 7.5% normal donkey serum for 1 h to block nonspecific protein binding, after which they were incubated overnight at 4°C with the following primary antibodies: anti-vWF (Dako, Carpinteria, CA; Cat. No. A0082, dilution 1:750), anti-phospho-signal transducer and activator of transcription (STAT)3 (Cell Signaling, Danvers, MA; Cat. No. 9145, dilution 1:150), anti-fibroblast growth factor receptor 1 (FGFR1) (Abcam; Cat. No. ab63601, dilution 1:150), anti-cleaved caspase 3 (Biocare Medical, Pacheco, CA; Cat. No. PP 229 AA, prediluted), and anti-Ki67 (Abcam; Cat. No. ab15580, dilution 1:1,000). .. The Ki67 antibody was detected by incubation with biotinylated anti-rabbit secondary (Vector, Burlingame, CA, dilution 1:200), followed by horseradish peroxidase (Vector, Cat. No. PK-6100).

Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
Article Snippet: .. The lung sections were then incubated with 7.5% normal donkey serum for 1 h to block nonspecific protein binding, after which they were incubated overnight at 4°C with the following primary antibodies: anti-vWF (Dako, Carpinteria, CA; Cat. No. A0082, dilution 1:750), anti-phospho-signal transducer and activator of transcription (STAT)3 (Cell Signaling, Danvers, MA; Cat. No. 9145, dilution 1:150), anti-fibroblast growth factor receptor 1 (FGFR1) (Abcam; Cat. No. ab63601, dilution 1:150), anti-cleaved caspase 3 (Biocare Medical, Pacheco, CA; Cat. No. PP 229 AA, prediluted), and anti-Ki67 (Abcam; Cat. No. ab15580, dilution 1:1,000). .. The Ki67 antibody was detected by incubation with biotinylated anti-rabbit secondary (Vector, Burlingame, CA, dilution 1:200), followed by horseradish peroxidase (Vector, Cat. No. PK-6100).

Staining:

Article Title: A Model of Gene-Environment Interaction Reveals Altered Mammary Gland Gene Expression and Increased Tumor Growth following Social Isolation
Article Snippet: .. Following antibody optimization, sections were prepared as described previously ( ) and stained with the following antibodies: anti–estrogen receptor α (ERα; 1:100, Santa Cruz Biotechnology), anti–progesterone receptor (PR; 1:200, Lab Vision Corporation), anti-GR (1:200, Santa Cruz Biotechnology), anti-CD31 (1:200, Santa Cruz Biotechnology), anti-Ki67 (1:200, Lab Vision Corporation), and anti–cleaved caspase-3 (1:50, Biocare Medical). ..

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  • 89
    Biocare Medical cleaved caspase 3 staining
    Enterocyte-specific SIRT1 deletion increases the rate of apoptosis in the intestinal tumors of APC +/min mice. (A) A representative western blot showing expression of the wild-type SIRT1 protein in the intestinal epithelium of APC +/min SIRT1 +/+ mice (first lane) and a truncated version in the epithelium of APC +/min SIRT1 −/− mice (second lane). Liver cells of the APC +/min SIRT1 −/− mice (third lane), as well as other tissues (not shown) express the wild-type protein. Pan-actin immunostaining served as a loading control. (B) Representative photographs of unfixed small intestines (distal segments) showing similar polyp number for APC +/min SIRT1 +/+ (right) and APC +/min SIRT1 −/− (left) mice. Scale bar indicates 5 mm length. (C) Representative photomicrographs of typical polyps from the two groups of mice, stained with hematoxylin and eosin. Scale bar indicates 100 µm length. (D) Representative photomicrographs and a bar graph showing Ki-67 immunohistochemical staining of polyp sections from APC +/min SIRT1 +/+ (left) and APC +/min SIRT1 −/− (middle) mice. Scale bar indicates 100 µm length. Proliferation index for polyps from APC +/min SIRT1 +/+ and APC +/min SIRT1 −/− mice (right), expressed as a fraction of Ki-67 positive cells within each polyp. Bars represent means ± SEM, n = 20 polyps per group. No statistically significant difference was observed. (E) Representative photomicrographs and a bar graph showing activated (cleaved) <t>caspase-3</t> immunohistochemical staining of polyp sections from APC +/min SIRT1 +/+ (left) and APC +/min SIRT1 −/− (middle) mice. Scale bar indicates 100 µm length. Absolute numbers of apoptotic (caspase-3 positive) cells per high power field (400 x) for polyps from SIRT1 +/+ and SIRT1 −/− mice (right). Bars represent means ± SEM, n = 25 polyps per group. ***p
    Cleaved Caspase 3 Staining, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3 staining/product/Biocare Medical
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 staining - by Bioz Stars, 2020-09
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    92
    Biocare Medical anti caspase 3 antibody
    Immunohistochemical analysis of mouse kidneys. Mice implanted intraperitoneally with allogeneic mesenchymal stromal cells (MSCs), Epo-MSCs, or Vehicle only, 1 day following cisplatin administration, were killed 4 days later and kidneys removed, sectioned and stained for detection of <t>Caspase-3</t> and Ki-67. ( a ) Caspase-3 (top panel) or Ki-67 (lower panel) expressing cells are indicated by black arrows in representative images. ( b ) The percentages of Caspase-3 positive cells and ( c ) Ki-67 positive cells per mm 2 kidney area were also evaluated as described in the Materials and Methods ( n = 4–6 mice/group, mean ± SEM). Normal mice not given cisplatin were used as controls.
    Anti Caspase 3 Antibody, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 3 antibody/product/Biocare Medical
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 antibody - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Biocare Medical rabbit anti cleaved caspase 3 antibody
    ( A) Percentage of Ki67 positive cells in control and EgKI-1-treated tumor sections, microscopy images showing tumor tissue sections of (B) control and (C) treated mice (D) Percentage of <t>caspase-3</t> positive cells in control and EgKI-1-treated tumor sections, microscopy images showing tumor tissue sections of (E) control and (F) treated mice, *for 0.005 ≤ p
    Rabbit Anti Cleaved Caspase 3 Antibody, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cleaved caspase 3 antibody/product/Biocare Medical
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cleaved caspase 3 antibody - by Bioz Stars, 2020-09
    92/100 stars
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    92
    Biocare Medical anti cleaved caspase 3
    TLR4 deficiency results in decreased neuronal apoptosis in the HF in response to GCI/R Neuronal apoptosis was confirmed by the methods of TUNEL staining (A to F) and IHC staining for cleaved <t>caspase-3,</t> a specific marker of apoptosis (G to L). The positive staining was shown in brown. Slides were counterstained with hematoxylin. Results show that GCI/R results in increased numbers of TUNEL positive cells in the HF (B and E) in WT mice. In TLR4 -/- -I/R mice, there were fewer TUNEL positive staining cells in the HF (C and F) compared with WT-I/R mice (M, # p
    Anti Cleaved Caspase 3, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3/product/Biocare Medical
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Enterocyte-specific SIRT1 deletion increases the rate of apoptosis in the intestinal tumors of APC +/min mice. (A) A representative western blot showing expression of the wild-type SIRT1 protein in the intestinal epithelium of APC +/min SIRT1 +/+ mice (first lane) and a truncated version in the epithelium of APC +/min SIRT1 −/− mice (second lane). Liver cells of the APC +/min SIRT1 −/− mice (third lane), as well as other tissues (not shown) express the wild-type protein. Pan-actin immunostaining served as a loading control. (B) Representative photographs of unfixed small intestines (distal segments) showing similar polyp number for APC +/min SIRT1 +/+ (right) and APC +/min SIRT1 −/− (left) mice. Scale bar indicates 5 mm length. (C) Representative photomicrographs of typical polyps from the two groups of mice, stained with hematoxylin and eosin. Scale bar indicates 100 µm length. (D) Representative photomicrographs and a bar graph showing Ki-67 immunohistochemical staining of polyp sections from APC +/min SIRT1 +/+ (left) and APC +/min SIRT1 −/− (middle) mice. Scale bar indicates 100 µm length. Proliferation index for polyps from APC +/min SIRT1 +/+ and APC +/min SIRT1 −/− mice (right), expressed as a fraction of Ki-67 positive cells within each polyp. Bars represent means ± SEM, n = 20 polyps per group. No statistically significant difference was observed. (E) Representative photomicrographs and a bar graph showing activated (cleaved) caspase-3 immunohistochemical staining of polyp sections from APC +/min SIRT1 +/+ (left) and APC +/min SIRT1 −/− (middle) mice. Scale bar indicates 100 µm length. Absolute numbers of apoptotic (caspase-3 positive) cells per high power field (400 x) for polyps from SIRT1 +/+ and SIRT1 −/− mice (right). Bars represent means ± SEM, n = 25 polyps per group. ***p

    Journal: PLoS ONE

    Article Title: Enterocyte-Specific Inactivation of SIRT1 Reduces Tumor Load in the APC+/min Mouse Model

    doi: 10.1371/journal.pone.0066283

    Figure Lengend Snippet: Enterocyte-specific SIRT1 deletion increases the rate of apoptosis in the intestinal tumors of APC +/min mice. (A) A representative western blot showing expression of the wild-type SIRT1 protein in the intestinal epithelium of APC +/min SIRT1 +/+ mice (first lane) and a truncated version in the epithelium of APC +/min SIRT1 −/− mice (second lane). Liver cells of the APC +/min SIRT1 −/− mice (third lane), as well as other tissues (not shown) express the wild-type protein. Pan-actin immunostaining served as a loading control. (B) Representative photographs of unfixed small intestines (distal segments) showing similar polyp number for APC +/min SIRT1 +/+ (right) and APC +/min SIRT1 −/− (left) mice. Scale bar indicates 5 mm length. (C) Representative photomicrographs of typical polyps from the two groups of mice, stained with hematoxylin and eosin. Scale bar indicates 100 µm length. (D) Representative photomicrographs and a bar graph showing Ki-67 immunohistochemical staining of polyp sections from APC +/min SIRT1 +/+ (left) and APC +/min SIRT1 −/− (middle) mice. Scale bar indicates 100 µm length. Proliferation index for polyps from APC +/min SIRT1 +/+ and APC +/min SIRT1 −/− mice (right), expressed as a fraction of Ki-67 positive cells within each polyp. Bars represent means ± SEM, n = 20 polyps per group. No statistically significant difference was observed. (E) Representative photomicrographs and a bar graph showing activated (cleaved) caspase-3 immunohistochemical staining of polyp sections from APC +/min SIRT1 +/+ (left) and APC +/min SIRT1 −/− (middle) mice. Scale bar indicates 100 µm length. Absolute numbers of apoptotic (caspase-3 positive) cells per high power field (400 x) for polyps from SIRT1 +/+ and SIRT1 −/− mice (right). Bars represent means ± SEM, n = 25 polyps per group. ***p

    Article Snippet: The rolls were then switched over into 70% ethanol and submitted for paraffin-embedding and hematoxylin/eosin, Ki-67 (rat anti-mouse Ki67, Dako, M7249, 1∶50 dilution) and cleaved caspase-3 staining (rabbit anti-mouse, Biocare Medical, CP 229B, 1∶50 dilution).

    Techniques: Mouse Assay, Western Blot, Expressing, Immunostaining, Staining, Immunohistochemistry

    Immunohistochemical analysis of mouse kidneys. Mice implanted intraperitoneally with allogeneic mesenchymal stromal cells (MSCs), Epo-MSCs, or Vehicle only, 1 day following cisplatin administration, were killed 4 days later and kidneys removed, sectioned and stained for detection of Caspase-3 and Ki-67. ( a ) Caspase-3 (top panel) or Ki-67 (lower panel) expressing cells are indicated by black arrows in representative images. ( b ) The percentages of Caspase-3 positive cells and ( c ) Ki-67 positive cells per mm 2 kidney area were also evaluated as described in the Materials and Methods ( n = 4–6 mice/group, mean ± SEM). Normal mice not given cisplatin were used as controls.

    Journal: Molecular Therapy

    Article Title: Erythropoietin Gene-enhanced Marrow Mesenchymal Stromal Cells Decrease Cisplatin-induced Kidney Injury and Improve Survival of Allogeneic Mice

    doi: 10.1038/mt.2011.162

    Figure Lengend Snippet: Immunohistochemical analysis of mouse kidneys. Mice implanted intraperitoneally with allogeneic mesenchymal stromal cells (MSCs), Epo-MSCs, or Vehicle only, 1 day following cisplatin administration, were killed 4 days later and kidneys removed, sectioned and stained for detection of Caspase-3 and Ki-67. ( a ) Caspase-3 (top panel) or Ki-67 (lower panel) expressing cells are indicated by black arrows in representative images. ( b ) The percentages of Caspase-3 positive cells and ( c ) Ki-67 positive cells per mm 2 kidney area were also evaluated as described in the Materials and Methods ( n = 4–6 mice/group, mean ± SEM). Normal mice not given cisplatin were used as controls.

    Article Snippet: The anti-Caspase-3 antibody (Biocare Medical; Concord, CA), diluted 1:100 with PBS, was incubated at room temperature for 2 hours.

    Techniques: Immunohistochemistry, Mouse Assay, Staining, Expressing

    Effect of mesenchymal stromal cells (MSCs) and Epo-MSCs conditioned media (CM) on protein expression in kidney cells. MM55.K mouse kidney epithelial cells were exposed, as described in Materials and Methods, to cisplatin at 7.5 µg/ml in the presence of CM from MSCs, CM from Epo-MSCs, or complete media for 42 hours. All kidney cells were then recovered, lysed, and used for Western blot analysis to assess expression of ( a ) Cleaved Caspase-3, ( b ) Cleaved Caspase-7, ( c ) Bcl-2, and ( d ) Bcl-xl, with β-tubulin levels used as internal control. The experiment was conducted three separate times and mean ± SEM values for fold change shown under representative blot for each protein.

    Journal: Molecular Therapy

    Article Title: Erythropoietin Gene-enhanced Marrow Mesenchymal Stromal Cells Decrease Cisplatin-induced Kidney Injury and Improve Survival of Allogeneic Mice

    doi: 10.1038/mt.2011.162

    Figure Lengend Snippet: Effect of mesenchymal stromal cells (MSCs) and Epo-MSCs conditioned media (CM) on protein expression in kidney cells. MM55.K mouse kidney epithelial cells were exposed, as described in Materials and Methods, to cisplatin at 7.5 µg/ml in the presence of CM from MSCs, CM from Epo-MSCs, or complete media for 42 hours. All kidney cells were then recovered, lysed, and used for Western blot analysis to assess expression of ( a ) Cleaved Caspase-3, ( b ) Cleaved Caspase-7, ( c ) Bcl-2, and ( d ) Bcl-xl, with β-tubulin levels used as internal control. The experiment was conducted three separate times and mean ± SEM values for fold change shown under representative blot for each protein.

    Article Snippet: The anti-Caspase-3 antibody (Biocare Medical; Concord, CA), diluted 1:100 with PBS, was incubated at room temperature for 2 hours.

    Techniques: Expressing, Western Blot

    ( A) Percentage of Ki67 positive cells in control and EgKI-1-treated tumor sections, microscopy images showing tumor tissue sections of (B) control and (C) treated mice (D) Percentage of caspase-3 positive cells in control and EgKI-1-treated tumor sections, microscopy images showing tumor tissue sections of (E) control and (F) treated mice, *for 0.005 ≤ p

    Journal: Scientific Reports

    Article Title: Kunitz type protease inhibitor from the canine tapeworm as a potential therapeutic for melanoma

    doi: 10.1038/s41598-019-52609-4

    Figure Lengend Snippet: ( A) Percentage of Ki67 positive cells in control and EgKI-1-treated tumor sections, microscopy images showing tumor tissue sections of (B) control and (C) treated mice (D) Percentage of caspase-3 positive cells in control and EgKI-1-treated tumor sections, microscopy images showing tumor tissue sections of (E) control and (F) treated mice, *for 0.005 ≤ p

    Article Snippet: Normal goat serum (10%) was then applied for 30 min and rabbit anti-cleaved Caspase-3 antibody (1:100) (Biocare Medical) applied for 2 hours at room temperature.

    Techniques: Microscopy, Mouse Assay

    TLR4 deficiency results in decreased neuronal apoptosis in the HF in response to GCI/R Neuronal apoptosis was confirmed by the methods of TUNEL staining (A to F) and IHC staining for cleaved caspase-3, a specific marker of apoptosis (G to L). The positive staining was shown in brown. Slides were counterstained with hematoxylin. Results show that GCI/R results in increased numbers of TUNEL positive cells in the HF (B and E) in WT mice. In TLR4 -/- -I/R mice, there were fewer TUNEL positive staining cells in the HF (C and F) compared with WT-I/R mice (M, # p

    Journal: Journal of neuroimmunology

    Article Title: Activation of Toll-like Receptor 4 Signaling Contributes to Hippocampal Neuronal Death Following Global Cerebral Ischemia/Reperfusion

    doi: 10.1016/j.jneuroim.2007.08.014

    Figure Lengend Snippet: TLR4 deficiency results in decreased neuronal apoptosis in the HF in response to GCI/R Neuronal apoptosis was confirmed by the methods of TUNEL staining (A to F) and IHC staining for cleaved caspase-3, a specific marker of apoptosis (G to L). The positive staining was shown in brown. Slides were counterstained with hematoxylin. Results show that GCI/R results in increased numbers of TUNEL positive cells in the HF (B and E) in WT mice. In TLR4 -/- -I/R mice, there were fewer TUNEL positive staining cells in the HF (C and F) compared with WT-I/R mice (M, # p

    Article Snippet: The primary antibodies used in the present study were anti-TLR4 (1:1000, gift from R. Medzhitov, Yale University), anti-p-NFκB-p65 (1:50, #3037, Cell Signaling Technology, Inc.) and anti-cleaved caspase-3 (1:50, CP229, Biocare Medical, Concord CA 94520).

    Techniques: TUNEL Assay, Staining, Immunohistochemistry, Marker, Mouse Assay