anti cleaved caspase 3  (Abcam)

 
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    Anti Caspase 3 antibody E87
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    ab32351
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    Structured Review

    Abcam anti cleaved caspase 3
    miR-21 can reduce PC12 cell death and TRPM7 is a functional target of miR-21 (A) . MiR-21 transfection efficiency detected by qPCR (B) . Apoptosis rate of PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (C) . Fluo-4 assay of PC12 cells (D) . The expression level of TRPM7 in PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (E) . Target region of miR-21 and TRPM7 (F) . Luciferase assay for the combination of miR-21 and TRPM7 (G) . Western blot analyzing the expression level of TRPM7, p65, and p-IκB proteins (H) . Quantitative histogram of TRPM7 expression level in different groups (I) . Western blot analyzing the expression level of cleaved <t>caspase-3.</t> * p

    https://www.bioz.com/result/anti cleaved caspase 3/product/Abcam
    Average 99 stars, based on 118 article reviews
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    Images

    1) Product Images from "MicroRNA-21 Overexpression Promotes the Neuroprotective Efficacy of Mesenchymal Stem Cells for Treatment of Intracerebral Hemorrhage"

    Article Title: MicroRNA-21 Overexpression Promotes the Neuroprotective Efficacy of Mesenchymal Stem Cells for Treatment of Intracerebral Hemorrhage

    Journal: Frontiers in Neurology

    doi: 10.3389/fneur.2018.00931

    miR-21 can reduce PC12 cell death and TRPM7 is a functional target of miR-21 (A) . MiR-21 transfection efficiency detected by qPCR (B) . Apoptosis rate of PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (C) . Fluo-4 assay of PC12 cells (D) . The expression level of TRPM7 in PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (E) . Target region of miR-21 and TRPM7 (F) . Luciferase assay for the combination of miR-21 and TRPM7 (G) . Western blot analyzing the expression level of TRPM7, p65, and p-IκB proteins (H) . Quantitative histogram of TRPM7 expression level in different groups (I) . Western blot analyzing the expression level of cleaved caspase-3. * p
    Figure Legend Snippet: miR-21 can reduce PC12 cell death and TRPM7 is a functional target of miR-21 (A) . MiR-21 transfection efficiency detected by qPCR (B) . Apoptosis rate of PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (C) . Fluo-4 assay of PC12 cells (D) . The expression level of TRPM7 in PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (E) . Target region of miR-21 and TRPM7 (F) . Luciferase assay for the combination of miR-21 and TRPM7 (G) . Western blot analyzing the expression level of TRPM7, p65, and p-IκB proteins (H) . Quantitative histogram of TRPM7 expression level in different groups (I) . Western blot analyzing the expression level of cleaved caspase-3. * p

    Techniques Used: Functional Assay, Transfection, Real-time Polymerase Chain Reaction, Expressing, Luciferase, Western Blot

    miR-21 enhanced survival of Hemin-induced mesenchymal stem cells (MSCs) (A) . Viability of MSCs treated with Hemin for different lengths of time (B) . Expression level of miR-21 detected by qPCR (C) . Viability of MSCs transfected with miR-21 mimics, miR-21 inhibitors, or their corresponding negative control (NC) (D) . Cell apoptosis analyzed by Annexin V-FITC and PI staining of MSCs in different groups (E) . Western blot analysis of cleaved-caspase-3 expression level in MSCs transfected with different miRNAs. * p
    Figure Legend Snippet: miR-21 enhanced survival of Hemin-induced mesenchymal stem cells (MSCs) (A) . Viability of MSCs treated with Hemin for different lengths of time (B) . Expression level of miR-21 detected by qPCR (C) . Viability of MSCs transfected with miR-21 mimics, miR-21 inhibitors, or their corresponding negative control (NC) (D) . Cell apoptosis analyzed by Annexin V-FITC and PI staining of MSCs in different groups (E) . Western blot analysis of cleaved-caspase-3 expression level in MSCs transfected with different miRNAs. * p

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Transfection, Negative Control, Staining, Western Blot

    2) Product Images from "Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation"

    Article Title: Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation

    Journal: Toxins

    doi: 10.3390/toxins10060235

    Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved caspase-3 in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p
    Figure Legend Snippet: Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved caspase-3 in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p

    Techniques Used: Expressing, Cell Culture, Western Blot

    3) Product Images from "Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98"

    Article Title: Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-019-1361-3

    Knockdown of linc00665 promotes cell cycle arrest and induces cell apoptosis in vitro. a , b Flow cytometric analysis of cell cycle distributions in A549 and H1299 cells after transfection with linc00665 siRNA. c , d Flow cytometric analysis of apoptosis in A549 and H1299 cells after transfection. e Expression of Bcl-2, Bax, Caspase-3, PARP, and GAPDH protein in A549 and H1299 cells after transfection with linc00665 siRNA. NC negative control; * p
    Figure Legend Snippet: Knockdown of linc00665 promotes cell cycle arrest and induces cell apoptosis in vitro. a , b Flow cytometric analysis of cell cycle distributions in A549 and H1299 cells after transfection with linc00665 siRNA. c , d Flow cytometric analysis of apoptosis in A549 and H1299 cells after transfection. e Expression of Bcl-2, Bax, Caspase-3, PARP, and GAPDH protein in A549 and H1299 cells after transfection with linc00665 siRNA. NC negative control; * p

    Techniques Used: In Vitro, Flow Cytometry, Transfection, Expressing, Negative Control

    4) Product Images from "Silencing of heart and neural crest derivatives expressed transcript 2 attenuates transforming growth factor-β1-enhanced apoptosis of human bronchial epithelial cells"

    Article Title: Silencing of heart and neural crest derivatives expressed transcript 2 attenuates transforming growth factor-β1-enhanced apoptosis of human bronchial epithelial cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.9299

    siHAND2 restores the expression levels of cell cycle- and apoptosis-related factors in HBE cells treated with TGF-β1 (A) Reverse transcription-quantitative polymerase chain reaction analysis was performed to detect mRNA expression of cell cycle- and apoptosis-related factors in HBE cells of different groups. The mRNA expression of cell cycle-related factors (Cyclin D1 and P21) decreased, and apoptosis-related factors (caspase-3, caspase-8 and caspase-9) increased significantly in the TGF-β1 group, compared with those in the control group. All expression levels were recovered in the siHAND2 + TGF-β1 group, compared with those in the TGF-β1 group (P
    Figure Legend Snippet: siHAND2 restores the expression levels of cell cycle- and apoptosis-related factors in HBE cells treated with TGF-β1 (A) Reverse transcription-quantitative polymerase chain reaction analysis was performed to detect mRNA expression of cell cycle- and apoptosis-related factors in HBE cells of different groups. The mRNA expression of cell cycle-related factors (Cyclin D1 and P21) decreased, and apoptosis-related factors (caspase-3, caspase-8 and caspase-9) increased significantly in the TGF-β1 group, compared with those in the control group. All expression levels were recovered in the siHAND2 + TGF-β1 group, compared with those in the TGF-β1 group (P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    5) Product Images from "CASC2/miR-24/miR-221 modulates the TRAIL resistance of hepatocellular carcinoma cell through caspase-8/caspase-3"

    Article Title: CASC2/miR-24/miR-221 modulates the TRAIL resistance of hepatocellular carcinoma cell through caspase-8/caspase-3

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0350-2

    CASC2 modulates TRAIL resistance through miR-24/miR-221 a-b  HepG2R and Bel-7402R cells were co-transfected with si-CASC2 and miR-24 inhibitor or miR-221 inhibitor upon TRAIL treatment; the cell viability was determined using MTT assays.  c-d  The protein levels of caspase 8 and cleaved-caspase 8 in si-CASC2 and miR-24 inhibitor co-transfected cells, and the protein levels of caspase 3 and cleaved-caspase 3 in si-CASC2 and miR-221 inhibitor co-transfected cells were determined using Western blot assays.  e-f  The cell apoptosis was determined using flow cytometer assays. The data are presented as mean ± SD of three independent experiments. * P
    Figure Legend Snippet: CASC2 modulates TRAIL resistance through miR-24/miR-221 a-b HepG2R and Bel-7402R cells were co-transfected with si-CASC2 and miR-24 inhibitor or miR-221 inhibitor upon TRAIL treatment; the cell viability was determined using MTT assays. c-d The protein levels of caspase 8 and cleaved-caspase 8 in si-CASC2 and miR-24 inhibitor co-transfected cells, and the protein levels of caspase 3 and cleaved-caspase 3 in si-CASC2 and miR-221 inhibitor co-transfected cells were determined using Western blot assays. e-f The cell apoptosis was determined using flow cytometer assays. The data are presented as mean ± SD of three independent experiments. * P

    Techniques Used: Transfection, MTT Assay, Western Blot, Flow Cytometry, Cytometry

    Screening and identification of candidate miRNAs related to TRAIL resistance of hepatocellular carcinoma a  Regular TRAIL-sensitive HepG2S and Bel-7402S cells (S stands for sensitive) and irregular TRAIL-resistant HepG2R and Bel-7402R cells (R stands for resistant) were treated with a series of doses of TRAIL (1, 10, 100, 1000 ng/ml); the cell viability was determined using MTT assays. Data were shown as a percentage normalized to the viability of cells with no TRAIL treatment. The abscissa was the logarithm of TRAIL concentration (log-conc.). IC50 represented the concentration of TRAIL when cell viability was reduced to 50%.  b  The mRNA expression of caspase 3 and caspase 8 were determined using real-time PCR assays.  c  The protein levels of caspase 3 and caspase 8 were determined using Western blot assays.  d  Online tools (Targetscan, RNA22, miRWalk, and Starbase) were used to search for candidate miRNAs that may regulate caspase 3 or caspase 8, respectively. Non-down-regulated miRNAs in TRAIL-resistant tumor cells were selected in conjunction with the chip data of the GEO database; furthermore, the previously reported miRNAs that inhibited hepatocarcinoma proliferation were excluded according to the literature in NCBI.  e – f  Caspase 3 or caspase 8 mRNA expression in response to miRNA overexpression was determined using real-time PCR assays. The data are presented as mean ± SD of three independent experiments. * P
    Figure Legend Snippet: Screening and identification of candidate miRNAs related to TRAIL resistance of hepatocellular carcinoma a Regular TRAIL-sensitive HepG2S and Bel-7402S cells (S stands for sensitive) and irregular TRAIL-resistant HepG2R and Bel-7402R cells (R stands for resistant) were treated with a series of doses of TRAIL (1, 10, 100, 1000 ng/ml); the cell viability was determined using MTT assays. Data were shown as a percentage normalized to the viability of cells with no TRAIL treatment. The abscissa was the logarithm of TRAIL concentration (log-conc.). IC50 represented the concentration of TRAIL when cell viability was reduced to 50%. b The mRNA expression of caspase 3 and caspase 8 were determined using real-time PCR assays. c The protein levels of caspase 3 and caspase 8 were determined using Western blot assays. d Online tools (Targetscan, RNA22, miRWalk, and Starbase) were used to search for candidate miRNAs that may regulate caspase 3 or caspase 8, respectively. Non-down-regulated miRNAs in TRAIL-resistant tumor cells were selected in conjunction with the chip data of the GEO database; furthermore, the previously reported miRNAs that inhibited hepatocarcinoma proliferation were excluded according to the literature in NCBI. e – f Caspase 3 or caspase 8 mRNA expression in response to miRNA overexpression was determined using real-time PCR assays. The data are presented as mean ± SD of three independent experiments. * P

    Techniques Used: MTT Assay, Concentration Assay, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Chromatin Immunoprecipitation, Over Expression

    MiR-24/miR-221 negatively regulate Caspase 8/3 through direct targeting a–b  Wild-type (wt-) and mutated-type (mut-) luciferase reporter gene vectors (wt-caspase 3 named wt-CASP3, wt-caspase 8 named wt-CASP8, mut-caspase 3 named mut-CASP3, mut-caspase 8 named mut-CASP8) were constructed. Mut-CASP3 contains a 5 bp mutation in the predicted miR-221 binding site; mut-CASP8 contains a 5 bp mutation in the predicted miR-24 binding site. The above vectors were co-transfected into HEK293 cells with miR-24/miR-221 mimics or inhibitor; the luciferase activity was determined.  c–d  The mimics or inhibitor of miR-24 and miR-221 was transfected into HepG2R and Bel-7402R cells, respectively; the protein levels of caspase 3 or caspase 8 were determined using Western blot assays. The data are presented as mean ± SD of three independent experiments. * P
    Figure Legend Snippet: MiR-24/miR-221 negatively regulate Caspase 8/3 through direct targeting a–b Wild-type (wt-) and mutated-type (mut-) luciferase reporter gene vectors (wt-caspase 3 named wt-CASP3, wt-caspase 8 named wt-CASP8, mut-caspase 3 named mut-CASP3, mut-caspase 8 named mut-CASP8) were constructed. Mut-CASP3 contains a 5 bp mutation in the predicted miR-221 binding site; mut-CASP8 contains a 5 bp mutation in the predicted miR-24 binding site. The above vectors were co-transfected into HEK293 cells with miR-24/miR-221 mimics or inhibitor; the luciferase activity was determined. c–d The mimics or inhibitor of miR-24 and miR-221 was transfected into HepG2R and Bel-7402R cells, respectively; the protein levels of caspase 3 or caspase 8 were determined using Western blot assays. The data are presented as mean ± SD of three independent experiments. * P

    Techniques Used: Luciferase, Construct, Mutagenesis, Binding Assay, Transfection, Activity Assay, Western Blot

    6) Product Images from "miR-155 mediates inflammatory injury of hippocampal neuronal cells via the activation of microglia"

    Article Title: miR-155 mediates inflammatory injury of hippocampal neuronal cells via the activation of microglia

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2019.9917

    miR-155 modulates apoptosis-associated protein expression. Hippocampal neuronal cells were transfected with mimic control, miR-155 mimics, or treated with LPS. Western blot assays were conducted to evaluate the expression levels of Bax, Bcl-2, pro-caspase-3 and cleaved-caspase-3 in hippocampal neuronal cells. # P
    Figure Legend Snippet: miR-155 modulates apoptosis-associated protein expression. Hippocampal neuronal cells were transfected with mimic control, miR-155 mimics, or treated with LPS. Western blot assays were conducted to evaluate the expression levels of Bax, Bcl-2, pro-caspase-3 and cleaved-caspase-3 in hippocampal neuronal cells. # P

    Techniques Used: Expressing, Transfection, Western Blot

    7) Product Images from "Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress-induced macrophage apoptosis in apoE−/− mice"

    Article Title: Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress-induced macrophage apoptosis in apoE−/− mice

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2015.4708

    Visceral adipose tissue-derived serine protease inhibitor (vaspin) downregulates the expression levels of proteins associated with endoplasmic reticulum stress-induced apoptosis in macrophages. Activating transcription factor (ATF)6, C/EBP-homologous protein (CHOP), total (t)-c-Jun N-terminal kinases (JNK)1/2, phosphorylated (p)-JNK1/2, cleaved-caspase 12, cleaved-caspase 9, and cleaved-caspase 3 protein expression levels were determined by western blot analysis. Each band density was normalized to control. Data are presented as the mean ± standard error of the mean.  * P
    Figure Legend Snippet: Visceral adipose tissue-derived serine protease inhibitor (vaspin) downregulates the expression levels of proteins associated with endoplasmic reticulum stress-induced apoptosis in macrophages. Activating transcription factor (ATF)6, C/EBP-homologous protein (CHOP), total (t)-c-Jun N-terminal kinases (JNK)1/2, phosphorylated (p)-JNK1/2, cleaved-caspase 12, cleaved-caspase 9, and cleaved-caspase 3 protein expression levels were determined by western blot analysis. Each band density was normalized to control. Data are presented as the mean ± standard error of the mean. * P

    Techniques Used: Derivative Assay, Protease Inhibitor, Expressing, Western Blot

    8) Product Images from "Silencing of heart and neural crest derivatives expressed transcript 2 attenuates transforming growth factor-β1-enhanced apoptosis of human bronchial epithelial cells"

    Article Title: Silencing of heart and neural crest derivatives expressed transcript 2 attenuates transforming growth factor-β1-enhanced apoptosis of human bronchial epithelial cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.9299

    siHAND2 restores the expression levels of cell cycle- and apoptosis-related factors in HBE cells treated with TGF-β1 (A) Reverse transcription-quantitative polymerase chain reaction analysis was performed to detect mRNA expression of cell cycle- and apoptosis-related factors in HBE cells of different groups. The mRNA expression of cell cycle-related factors (Cyclin D1 and P21) decreased, and apoptosis-related factors (caspase-3, caspase-8 and caspase-9) increased significantly in the TGF-β1 group, compared with those in the control group. All expression levels were recovered in the siHAND2 + TGF-β1 group, compared with those in the TGF-β1 group (P
    Figure Legend Snippet: siHAND2 restores the expression levels of cell cycle- and apoptosis-related factors in HBE cells treated with TGF-β1 (A) Reverse transcription-quantitative polymerase chain reaction analysis was performed to detect mRNA expression of cell cycle- and apoptosis-related factors in HBE cells of different groups. The mRNA expression of cell cycle-related factors (Cyclin D1 and P21) decreased, and apoptosis-related factors (caspase-3, caspase-8 and caspase-9) increased significantly in the TGF-β1 group, compared with those in the control group. All expression levels were recovered in the siHAND2 + TGF-β1 group, compared with those in the TGF-β1 group (P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    9) Product Images from "Panobinostat, a histone deacetylase inhibitor, suppresses leptomeningeal seeding in a medulloblastoma animal model"

    Article Title: Panobinostat, a histone deacetylase inhibitor, suppresses leptomeningeal seeding in a medulloblastoma animal model

    Journal: Oncotarget

    doi: 10.18632/oncotarget.18132

    Effect of panobinostat on histone acetylation, apoptosis, cell cycle, IDs and differentiation-related signal pathway in medulloblastoma cells Immunoblotting of whole cell lysates prepared from MB cells treated with panobinostat. The representative western blot result shows panobinostat clearly regulated acetyl-Histone H3 (Ac-H3), cleaved caspase-3, CCND1, IDs, synaptophysin and NeuroD1.
    Figure Legend Snippet: Effect of panobinostat on histone acetylation, apoptosis, cell cycle, IDs and differentiation-related signal pathway in medulloblastoma cells Immunoblotting of whole cell lysates prepared from MB cells treated with panobinostat. The representative western blot result shows panobinostat clearly regulated acetyl-Histone H3 (Ac-H3), cleaved caspase-3, CCND1, IDs, synaptophysin and NeuroD1.

    Techniques Used: Western Blot

    Therapeutic effect of panobinostat in a UW426-effLuc medulloblastoma leptomeningeal seeding model UW426-effLuc mice were treated 3 days later with panobinostat (10 mg/kg once daily for 2 weeks). (A and B) Serial bioluminescence image (BLI) of representative mice in each group. Quantification of BLI results shows that panobinostat reduced intracranial tumor burden and inhibited spinal cord seeding. (C) Representative longitudinal sections of whole craniospinal neuraxis of the mice with UW426-effLuc. Immunofluorescence with acetyl-H3 (green), Ki-67 (red), cleaved caspase-3 (green), ID3 (green) and synaptophysin (red). Representative images reveal increased Ac-H3, cleaved caspase-3 and synaptophysin and decreased Ki-67 and ID3 in (D) cerebellar mass and (E) leptomeningeal disseminated tumor cell layers. Scale bars, 100 μm. Cells were counterstained with 4′,6′-diamidino-2-phenylindole (blue). (F) A plot of overall survival is estimated by the Kaplan-Meier method. Panobinostat treatment significantly enhances the survival of mice bearing UW426-effLuc. *p
    Figure Legend Snippet: Therapeutic effect of panobinostat in a UW426-effLuc medulloblastoma leptomeningeal seeding model UW426-effLuc mice were treated 3 days later with panobinostat (10 mg/kg once daily for 2 weeks). (A and B) Serial bioluminescence image (BLI) of representative mice in each group. Quantification of BLI results shows that panobinostat reduced intracranial tumor burden and inhibited spinal cord seeding. (C) Representative longitudinal sections of whole craniospinal neuraxis of the mice with UW426-effLuc. Immunofluorescence with acetyl-H3 (green), Ki-67 (red), cleaved caspase-3 (green), ID3 (green) and synaptophysin (red). Representative images reveal increased Ac-H3, cleaved caspase-3 and synaptophysin and decreased Ki-67 and ID3 in (D) cerebellar mass and (E) leptomeningeal disseminated tumor cell layers. Scale bars, 100 μm. Cells were counterstained with 4′,6′-diamidino-2-phenylindole (blue). (F) A plot of overall survival is estimated by the Kaplan-Meier method. Panobinostat treatment significantly enhances the survival of mice bearing UW426-effLuc. *p

    Techniques Used: Mouse Assay, Immunofluorescence

    10) Product Images from "Signalling through AMPA receptors on oligodendrocyte precursors promotes myelination by enhancing oligodendrocyte survival"

    Article Title: Signalling through AMPA receptors on oligodendrocyte precursors promotes myelination by enhancing oligodendrocyte survival

    Journal: eLife

    doi: 10.7554/eLife.28080

    Gria3 null 2 –/– 4 –/– mice generate fewer OLs in the corpus callosum. ( A ) CC1 immunolabelling marks differentiated OLs in Gria3 null and Gria3 null 2 –/– 4 –/– mice. ( B ) The density of CC1 + OLs is significantly less (by ∼22% at P14 and ~26% at P53) in Gria3 null 2 –/– 4 –/– mice relative to Gria3 null controls (p=0.026 at P14, p=0.029 at P53, Mann-Whitney test; > 900 cells were counted per mouse at all ages). ( C ) Pdgfra + OPs in Gria3 null and Gria3 null 2 –/– 4 –/– mice. ( D ) There were no significant differences in the density of Pdgfra + OPs at P14 or P53 in Gria3 null 2 –/– 4 –/– versus Gria3 null mice (p=0.11 at P14; p=0.7 at P53, Mann-Whitney test; > 400 cells were counted per mouse at all ages). ( E ) Cleaved Caspase-3 + , Olig2 + OL lineage cells in Gria3 null 2 –/– 4 –/– mice. Cells in the rectangle (dotted line) are shown on the right at higher magnification. ( F ) There was a ~24% increase in the fraction of Olig2 + cells that expressed cleaved Caspase-3 in Gria3 null 2 –/– 4 –/– compared to Gria3 null mice (p=0.004, Mann-Whitney test; > 1500 Olig2 + cells were counted in each mouse). ( G ) Dye-filled OLs in P14 corpus callosum of Gria3 null and Gria3 null 2 –/– 4 –/– mice. ( H ) There was no change in the length of the internodes (p=0.93, Student’s t-test) or the number of internodes per OL (p=0.41, Student’s t-test) in Gria3 null 2 –/– 4 –/– compared to Gria3 null mice. Numbers of mice analyzed are indicated in ( B ), ( D ) and ( F ) and numbers of cells in ( H ). Scale bars: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.28080.017
    Figure Legend Snippet: Gria3 null 2 –/– 4 –/– mice generate fewer OLs in the corpus callosum. ( A ) CC1 immunolabelling marks differentiated OLs in Gria3 null and Gria3 null 2 –/– 4 –/– mice. ( B ) The density of CC1 + OLs is significantly less (by ∼22% at P14 and ~26% at P53) in Gria3 null 2 –/– 4 –/– mice relative to Gria3 null controls (p=0.026 at P14, p=0.029 at P53, Mann-Whitney test; > 900 cells were counted per mouse at all ages). ( C ) Pdgfra + OPs in Gria3 null and Gria3 null 2 –/– 4 –/– mice. ( D ) There were no significant differences in the density of Pdgfra + OPs at P14 or P53 in Gria3 null 2 –/– 4 –/– versus Gria3 null mice (p=0.11 at P14; p=0.7 at P53, Mann-Whitney test; > 400 cells were counted per mouse at all ages). ( E ) Cleaved Caspase-3 + , Olig2 + OL lineage cells in Gria3 null 2 –/– 4 –/– mice. Cells in the rectangle (dotted line) are shown on the right at higher magnification. ( F ) There was a ~24% increase in the fraction of Olig2 + cells that expressed cleaved Caspase-3 in Gria3 null 2 –/– 4 –/– compared to Gria3 null mice (p=0.004, Mann-Whitney test; > 1500 Olig2 + cells were counted in each mouse). ( G ) Dye-filled OLs in P14 corpus callosum of Gria3 null and Gria3 null 2 –/– 4 –/– mice. ( H ) There was no change in the length of the internodes (p=0.93, Student’s t-test) or the number of internodes per OL (p=0.41, Student’s t-test) in Gria3 null 2 –/– 4 –/– compared to Gria3 null mice. Numbers of mice analyzed are indicated in ( B ), ( D ) and ( F ) and numbers of cells in ( H ). Scale bars: 50 μm. DOI: http://dx.doi.org/10.7554/eLife.28080.017

    Techniques Used: Mouse Assay, MANN-WHITNEY

    11) Product Images from "Sappanone A Protects Against Myocardial Ischemia Reperfusion Injury by Modulation of Nrf2"

    Article Title: Sappanone A Protects Against Myocardial Ischemia Reperfusion Injury by Modulation of Nrf2

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/DDDT.S230358

    Sappanone A (SA) inhibited ischemia reperfusion (IR)-induced myocardial apoptosis. SA (20 mg/kg) was intraperitoneally administrated into rats 1 h prior to heart isolation, and then the hearts were isolated that underwent 30-min ischemia, followed by 120-min reperfusion. ( A ) Myocardial apoptosis was measured by terminal deoxynucleotidyl transferase dUTP nick end labeling staining (x400) (n=6 per group). The apoptotic cell nuclei were stained brown and the living cell nuclei were stained blue. Scale bar=20 µm. ( B ) The expression of cleaved caspase-3 was measured by Western blotting (n=3). Data are expressed as the mean ± standard deviation (SD). *P
    Figure Legend Snippet: Sappanone A (SA) inhibited ischemia reperfusion (IR)-induced myocardial apoptosis. SA (20 mg/kg) was intraperitoneally administrated into rats 1 h prior to heart isolation, and then the hearts were isolated that underwent 30-min ischemia, followed by 120-min reperfusion. ( A ) Myocardial apoptosis was measured by terminal deoxynucleotidyl transferase dUTP nick end labeling staining (x400) (n=6 per group). The apoptotic cell nuclei were stained brown and the living cell nuclei were stained blue. Scale bar=20 µm. ( B ) The expression of cleaved caspase-3 was measured by Western blotting (n=3). Data are expressed as the mean ± standard deviation (SD). *P

    Techniques Used: Isolation, TUNEL Assay, Staining, Expressing, Western Blot, Standard Deviation

    12) Product Images from "Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells"

    Article Title: Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2013/159864

    Htra2 induction and cleaved caspase-3 activation were detected by Western blot. Experiments were repeated three times, and the data were presented as mean ± SD (* P
    Figure Legend Snippet: Htra2 induction and cleaved caspase-3 activation were detected by Western blot. Experiments were repeated three times, and the data were presented as mean ± SD (* P

    Techniques Used: Activation Assay, Western Blot

    13) Product Images from "Inhibition of autophagy enhances the selective anti-cancer activity of tigecycline to overcome drug resistance in the treatment of chronic myeloid leukemia"

    Article Title: Inhibition of autophagy enhances the selective anti-cancer activity of tigecycline to overcome drug resistance in the treatment of chronic myeloid leukemia

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-017-0512-6

    Tigecycline has no obvious inhibitory effect on normal cells. ( a ) Death of normal bone marrow mononuclear cells exposed to tigecycline in different time points were measured by flow cytometry. ( b ) Western blot analysis of cleaved caspase-3 protein level in normal cells. ( c ) Effect of tigecycline on the oxygen consumption rate (OCR) curves and values for CML cells and normal cells. ( d ) Analyses of the glycolysis capacity of leukemic and normal cells were performed before and after stimulation with tigecycline. ( e ) The mitochondrial membrane potentials of leukemic and normal cells before and after treatment with tigecycline. ( f ) Mitochondrial mass of normal cells and CML cells was measured by incubating the cells with MitoTracker® Green FM dye. ( g ) Mitochondrial DNA (mtDNA) copy number was analyzed by QPCR. * P
    Figure Legend Snippet: Tigecycline has no obvious inhibitory effect on normal cells. ( a ) Death of normal bone marrow mononuclear cells exposed to tigecycline in different time points were measured by flow cytometry. ( b ) Western blot analysis of cleaved caspase-3 protein level in normal cells. ( c ) Effect of tigecycline on the oxygen consumption rate (OCR) curves and values for CML cells and normal cells. ( d ) Analyses of the glycolysis capacity of leukemic and normal cells were performed before and after stimulation with tigecycline. ( e ) The mitochondrial membrane potentials of leukemic and normal cells before and after treatment with tigecycline. ( f ) Mitochondrial mass of normal cells and CML cells was measured by incubating the cells with MitoTracker® Green FM dye. ( g ) Mitochondrial DNA (mtDNA) copy number was analyzed by QPCR. * P

    Techniques Used: Flow Cytometry, Cytometry, Western Blot, Real-time Polymerase Chain Reaction

    Tigecycline causes apoptosis of CML cells by activating the cytochrome c/caspase-9/caspase-3 signaling pathway. ( a ) Apoptosis assay of the CML cells in response to stimulation with tigecycline. Left panel: a representative flow cytometry plots for CML cells stained with annexin V-FITC/PI-stained. Right panel: percentage apoptosis of the CML cells. Apoptosis was defined as the percentage of annexin V-positive cells. ( b ) Western blot analyses of cytochrome c, cleaved caspase-9, and caspase-3 protein levels. Cyto.C (Mito), cytochrome c protein in the mitochondria; Cyto.C (Cyto), cytochrome c protein in the cytoplasm. Cytochrome c oxidase-4 and β-actin were used as the reference proteins for the analyses of mitochondrial and cytoplasmic proteins, respectively. * P
    Figure Legend Snippet: Tigecycline causes apoptosis of CML cells by activating the cytochrome c/caspase-9/caspase-3 signaling pathway. ( a ) Apoptosis assay of the CML cells in response to stimulation with tigecycline. Left panel: a representative flow cytometry plots for CML cells stained with annexin V-FITC/PI-stained. Right panel: percentage apoptosis of the CML cells. Apoptosis was defined as the percentage of annexin V-positive cells. ( b ) Western blot analyses of cytochrome c, cleaved caspase-9, and caspase-3 protein levels. Cyto.C (Mito), cytochrome c protein in the mitochondria; Cyto.C (Cyto), cytochrome c protein in the cytoplasm. Cytochrome c oxidase-4 and β-actin were used as the reference proteins for the analyses of mitochondrial and cytoplasmic proteins, respectively. * P

    Techniques Used: Apoptosis Assay, Flow Cytometry, Cytometry, Staining, Western Blot

    14) Product Images from "Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis"

    Article Title: Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.102575

    The involvement of METTL1/miR-149-3p axis in the regulation of CR-CC cell apoptosis. ( A , B ) FCM was employed to determine the apoptosis ratio of CR-CC cells. ( C , D ) Western Blot was used to detect the expression levels of cleaved Caspase-3 in CR-HCT116 cells, CR-SW480 cells and CR-SW620 cells respectively. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+OE-MET” represented “Cisplatin plus overexpressed METTL1 treated group”, “Cis+OE-MET+Inhi” represented “Cisplatin plus overexpressed METTL1 and miR-149-3p inhibitor treated group”). All the experiments repeated at least 3 times. “*” means p
    Figure Legend Snippet: The involvement of METTL1/miR-149-3p axis in the regulation of CR-CC cell apoptosis. ( A , B ) FCM was employed to determine the apoptosis ratio of CR-CC cells. ( C , D ) Western Blot was used to detect the expression levels of cleaved Caspase-3 in CR-HCT116 cells, CR-SW480 cells and CR-SW620 cells respectively. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+OE-MET” represented “Cisplatin plus overexpressed METTL1 treated group”, “Cis+OE-MET+Inhi” represented “Cisplatin plus overexpressed METTL1 and miR-149-3p inhibitor treated group”). All the experiments repeated at least 3 times. “*” means p

    Techniques Used: Western Blot, Expressing

    The effects of S100A4 and p53 on CR-CC cell viability. ( A , B ) TUNEL assay was conducted to detect cell apoptosis ratio in CC cells. ( C , D ) Western Blot was used to determine the expression levels of cleaved Caspase-3 in CR-CC cells. CCK-8 assay was performed to detect the proliferation ability of ( E ) CR-HCT116 cells, ( F ) CR-SW480 cells and ( G ) CR-SW620 cells. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+KD-S” represented “Cisplatin plus downregulated S100A4 treated group”, “Cis+OE-p53” represented “Cisplatin plus overexpressed p53 treated group”). All the experiments repeated at least 3 times. “*” means p
    Figure Legend Snippet: The effects of S100A4 and p53 on CR-CC cell viability. ( A , B ) TUNEL assay was conducted to detect cell apoptosis ratio in CC cells. ( C , D ) Western Blot was used to determine the expression levels of cleaved Caspase-3 in CR-CC cells. CCK-8 assay was performed to detect the proliferation ability of ( E ) CR-HCT116 cells, ( F ) CR-SW480 cells and ( G ) CR-SW620 cells. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+KD-S” represented “Cisplatin plus downregulated S100A4 treated group”, “Cis+OE-p53” represented “Cisplatin plus overexpressed p53 treated group”). All the experiments repeated at least 3 times. “*” means p

    Techniques Used: TUNEL Assay, Western Blot, Expressing, CCK-8 Assay

    Overexpressed METTL1 promoted CR-CC cells apoptosis by regulating S100A4. ( A , B ) FCM was conducted to determine cell apoptosis ratio in CC cells. ( C , D ) Western Blot was employed to detect the expression levels of cleaved Caspase-3 in CR-CC cells. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+OE-MET” represented “Cisplatin plus overexpressed METTL1 treated group”, “Cis+OE-M+OE-S” represented “Cisplatin plus overexpressed METTL1 and overexpressed S100A4 treated group”). “*” means p
    Figure Legend Snippet: Overexpressed METTL1 promoted CR-CC cells apoptosis by regulating S100A4. ( A , B ) FCM was conducted to determine cell apoptosis ratio in CC cells. ( C , D ) Western Blot was employed to detect the expression levels of cleaved Caspase-3 in CR-CC cells. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+OE-MET” represented “Cisplatin plus overexpressed METTL1 treated group”, “Cis+OE-M+OE-S” represented “Cisplatin plus overexpressed METTL1 and overexpressed S100A4 treated group”). “*” means p

    Techniques Used: Western Blot, Expressing

    15) Product Images from "miR-23a binds to p53 and enhances its association with miR-128 promoter"

    Article Title: miR-23a binds to p53 and enhances its association with miR-128 promoter

    Journal: Scientific Reports

    doi: 10.1038/srep16422

    miR-23a promotes apoptosis. ( A , B , upper panel) The levels of cleaved caspase-3 and cleaved PARP were elevated in response to hydrogen peroxide and doxorubicin treatment. Cardiomyocytes were treated with 100 μM hydrogen peroxide (H 2 O 2 , A) or 1 μM doxorubicin (Dox, B), at indicated time and cleaved caspase-3 and cleaved PARP were analysed by immunoblot. The total amount of β-actin served as internal control. ( A , B , lower panel) miR-23a was elevated in response to apoptotic stimuli. Cardiomyocytes were treated with 100 μM hydrogen peroxide ( A ) or 1 μM doxorubicin ( B ), and harvested at the indicated time for the detection of miR-23a by qRT-PCR. *p
    Figure Legend Snippet: miR-23a promotes apoptosis. ( A , B , upper panel) The levels of cleaved caspase-3 and cleaved PARP were elevated in response to hydrogen peroxide and doxorubicin treatment. Cardiomyocytes were treated with 100 μM hydrogen peroxide (H 2 O 2 , A) or 1 μM doxorubicin (Dox, B), at indicated time and cleaved caspase-3 and cleaved PARP were analysed by immunoblot. The total amount of β-actin served as internal control. ( A , B , lower panel) miR-23a was elevated in response to apoptotic stimuli. Cardiomyocytes were treated with 100 μM hydrogen peroxide ( A ) or 1 μM doxorubicin ( B ), and harvested at the indicated time for the detection of miR-23a by qRT-PCR. *p

    Techniques Used: Quantitative RT-PCR

    16) Product Images from "miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR"

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S173416

    miR-376a overexpression inhibits breast cancer cell proliferation and promoted cell apoptosis. Notes: ( A and B ) MDA-MB-453 and MDA-MB-231 cells with miR-376a overexpression or not were subjected to analyze the cell viability by CCK8 assay. ( C and D ) Cell apoptosis was detected in cells depicted in ( A ). ( E ) The expression of apoptosis executors (cleaved caspase 3 and cleaved PARP) was examined in cells depicted in ( A ). Data are presented as mean±SD; * P
    Figure Legend Snippet: miR-376a overexpression inhibits breast cancer cell proliferation and promoted cell apoptosis. Notes: ( A and B ) MDA-MB-453 and MDA-MB-231 cells with miR-376a overexpression or not were subjected to analyze the cell viability by CCK8 assay. ( C and D ) Cell apoptosis was detected in cells depicted in ( A ). ( E ) The expression of apoptosis executors (cleaved caspase 3 and cleaved PARP) was examined in cells depicted in ( A ). Data are presented as mean±SD; * P

    Techniques Used: Over Expression, Multiple Displacement Amplification, CCK-8 Assay, Expressing

    Overexpression of NRP-1 or inhibition of Wnt/β-catenin signaling rescues the inhibitory effects of miR-376a on breast cancer cell progression. Notes: ( A and B ) The cell viability was examined in MDA-MB-453 and MDA-MB-231 cells with miR-376a overexpression as well as NRP-1 knockdown or SKL2001 treatment. ( C ) Cell migration ability was detected in cells depicted in ( A ). ( D ) The expression of epithelial (E-cadherin) and mesenchymal markers (Vimentin) was measured in cells depicted in ( A ). ( E ) Cell apoptosis was evaluated in cells depicted in ( A ). ( F ) The expression of apoptosis executors (cleaved caspase 3 and cleaved PARP) was determined in cells depicted in ( A ). Data are presented as mean±SD; * P
    Figure Legend Snippet: Overexpression of NRP-1 or inhibition of Wnt/β-catenin signaling rescues the inhibitory effects of miR-376a on breast cancer cell progression. Notes: ( A and B ) The cell viability was examined in MDA-MB-453 and MDA-MB-231 cells with miR-376a overexpression as well as NRP-1 knockdown or SKL2001 treatment. ( C ) Cell migration ability was detected in cells depicted in ( A ). ( D ) The expression of epithelial (E-cadherin) and mesenchymal markers (Vimentin) was measured in cells depicted in ( A ). ( E ) Cell apoptosis was evaluated in cells depicted in ( A ). ( F ) The expression of apoptosis executors (cleaved caspase 3 and cleaved PARP) was determined in cells depicted in ( A ). Data are presented as mean±SD; * P

    Techniques Used: Over Expression, Inhibition, Multiple Displacement Amplification, Migration, Expressing

    17) Product Images from "Tanshinone IIA Reverses Gefitinib-Resistance In Human Non-Small-Cell Lung Cancer Via Regulation Of VEGFR/Akt Pathway"

    Article Title: Tanshinone IIA Reverses Gefitinib-Resistance In Human Non-Small-Cell Lung Cancer Via Regulation Of VEGFR/Akt Pathway

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S221228

    Tan IIA enhances the pro-apoptotic effect of gefitinib in HCC827/gefitinib cells. ( A, B ) HCC827/gefitinib cells were treated with 40 nM OXA or/and 2 μM Tan IIA for 72 hrs. Apoptotic cells were measured by flow cytometry. ( C, D ) Expression level of active caspase 3 in HCC827/gefitinib cells was detected with Western blotting. *P
    Figure Legend Snippet: Tan IIA enhances the pro-apoptotic effect of gefitinib in HCC827/gefitinib cells. ( A, B ) HCC827/gefitinib cells were treated with 40 nM OXA or/and 2 μM Tan IIA for 72 hrs. Apoptotic cells were measured by flow cytometry. ( C, D ) Expression level of active caspase 3 in HCC827/gefitinib cells was detected with Western blotting. *P

    Techniques Used: Flow Cytometry, Cytometry, Expressing, Western Blot

    18) Product Images from "Olmesartan prevents cardiac rupture in mice with myocardial infarction by modulating growth differentiation factor 15 and p53"

    Article Title: Olmesartan prevents cardiac rupture in mice with myocardial infarction by modulating growth differentiation factor 15 and p53

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.12736

    RNH6270 inhibited the apoptotic signal in vitro . Western blots of p53, p-p53 and cleaved caspase 3 induced by Ang II in cultured neonatal rat cardiomyocytes (A) and fibroblasts (B) were performed, and the expression of each protein was normalized to β-actin
    Figure Legend Snippet: RNH6270 inhibited the apoptotic signal in vitro . Western blots of p53, p-p53 and cleaved caspase 3 induced by Ang II in cultured neonatal rat cardiomyocytes (A) and fibroblasts (B) were performed, and the expression of each protein was normalized to β-actin

    Techniques Used: In Vitro, Western Blot, Cell Culture, Expressing

    19) Product Images from "Synergy between hemagglutinin 2 (HA2) subunit of influenza fusogenic membrane glycoprotein and oncolytic Newcastle disease virus suppressed tumor growth and further enhanced by Immune checkpoint PD-1 blockade"

    Article Title: Synergy between hemagglutinin 2 (HA2) subunit of influenza fusogenic membrane glycoprotein and oncolytic Newcastle disease virus suppressed tumor growth and further enhanced by Immune checkpoint PD-1 blockade

    Journal: Cancer Cell International

    doi: 10.1186/s12935-020-01476-5

    Immunohistochemistry of caspase-3 expression in tumor tissue. a Tumor-bearing mice were treated with NDV alone or in combination with HA2 plasmid then sacrificed and tumor tissue was excised to process for IHC studies. Randomly selected areas from each tumor were analyzed. Arrowheads represent immunohistochemical staining of caspase-3 (magnification: ×200). b Comparison between the stained areas of caspase-3 expression using Image J software. Bar graphs indicate the mean percentage (%) of staining area in tumor section of different groups. Values represent the mean ± standard deviation (SD) of three independent experiments. **P
    Figure Legend Snippet: Immunohistochemistry of caspase-3 expression in tumor tissue. a Tumor-bearing mice were treated with NDV alone or in combination with HA2 plasmid then sacrificed and tumor tissue was excised to process for IHC studies. Randomly selected areas from each tumor were analyzed. Arrowheads represent immunohistochemical staining of caspase-3 (magnification: ×200). b Comparison between the stained areas of caspase-3 expression using Image J software. Bar graphs indicate the mean percentage (%) of staining area in tumor section of different groups. Values represent the mean ± standard deviation (SD) of three independent experiments. **P

    Techniques Used: Immunohistochemistry, Expressing, Mouse Assay, Plasmid Preparation, Staining, Software, Standard Deviation

    20) Product Images from "Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98"

    Article Title: Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-019-1361-3

    Knockdown of linc00665 promotes cell cycle arrest and induces cell apoptosis in vitro. a , b Flow cytometric analysis of cell cycle distributions in A549 and H1299 cells after transfection with linc00665 siRNA. c , d Flow cytometric analysis of apoptosis in A549 and H1299 cells after transfection. e Expression of Bcl-2, Bax, Caspase-3, PARP, and GAPDH protein in A549 and H1299 cells after transfection with linc00665 siRNA. NC negative control; * p
    Figure Legend Snippet: Knockdown of linc00665 promotes cell cycle arrest and induces cell apoptosis in vitro. a , b Flow cytometric analysis of cell cycle distributions in A549 and H1299 cells after transfection with linc00665 siRNA. c , d Flow cytometric analysis of apoptosis in A549 and H1299 cells after transfection. e Expression of Bcl-2, Bax, Caspase-3, PARP, and GAPDH protein in A549 and H1299 cells after transfection with linc00665 siRNA. NC negative control; * p

    Techniques Used: In Vitro, Flow Cytometry, Transfection, Expressing, Negative Control

    21) Product Images from "Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality"

    Article Title: Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2017.124

    Damaged livers and hearts in Pdcd5 –/– embryos at E12.5. ( a ) The livers from Pdcd5 +/+ or Pdcd5 –/– embryos were analyzed by H E staining. ( b–c ) The hearts from Pdcd5 +/+ or Pdcd5 –/– embryos were analyzed by H E staining, magnified by 100 × ( b ) and 400 × ( c ). ( d ) The levels of cleaved caspase-3 protein in Pdcd5 +/+ or Pdcd5 –/– livers were detected by immunohistochemical analysis
    Figure Legend Snippet: Damaged livers and hearts in Pdcd5 –/– embryos at E12.5. ( a ) The livers from Pdcd5 +/+ or Pdcd5 –/– embryos were analyzed by H E staining. ( b–c ) The hearts from Pdcd5 +/+ or Pdcd5 –/– embryos were analyzed by H E staining, magnified by 100 × ( b ) and 400 × ( c ). ( d ) The levels of cleaved caspase-3 protein in Pdcd5 +/+ or Pdcd5 –/– livers were detected by immunohistochemical analysis

    Techniques Used: Staining, Immunohistochemistry

    22) Product Images from "Down‐regulation of GAS5 ameliorates myocardial ischaemia/reperfusion injury via the miR‐335/ROCK1/AKT/GSK‐3β axis. Down‐regulation of GAS5 ameliorates myocardial ischaemia/reperfusion injury via the miR‐335/ROCK1/AKT/GSK‐3β axis"

    Article Title: Down‐regulation of GAS5 ameliorates myocardial ischaemia/reperfusion injury via the miR‐335/ROCK1/AKT/GSK‐3β axis. Down‐regulation of GAS5 ameliorates myocardial ischaemia/reperfusion injury via the miR‐335/ROCK1/AKT/GSK‐3β axis

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.14724

    Down‐regulation of GAS5 alleviated I/R‐induced myocardial injury ex vivo. A, Rats were given a single tail‐vein injection of adeno‐associated virus carrying small hairpin RNAs (AAV‐shGAS5) and negative control (AAV‐NC). Four weeks after the injection, the expression of GAS5 in left ventricular myocardium was determined using qRT‐PCR. B, Myocardial submicroscopic structure was observed by transmission electron microscope (×5000). C, The effect of GAS5 knockdown on myocardial infarct size measured by TTC staining (n = 6 per group). The viable myocardium was stained red, and infarct myocardium was unstained. D,The effect of GAS5 knockdown on the changes of heart rate, left ventricular developed pressure (LVDP), positive first‐order derivative of ventricular pressure (+dp/dt) and negative first‐order derivative of ventricular pressure (−dp/dt). Baseline: cardiac function parameters were recorded 1 min before ischaemia. R‐30, R‐60 and R‐120: cardiac function parameters were collected after 30, 60 and 120 min of reperfusion (n = 6 per group). E, The effect of GAS5 knockdown on apoptosis measured by TUNEL staining (×400) (n = 6 per group). The apoptotic cell nuclei were stained brown, and the living cell nuclei were stained blue. F, The effect of GAS5 knockdown on cleaved caspase‐3 protein expression measured by immunohistochemistry (IHC) analysis (×400) (n = 6 per group). Data are expressed as the mean ± standard deviation (SD). ** P
    Figure Legend Snippet: Down‐regulation of GAS5 alleviated I/R‐induced myocardial injury ex vivo. A, Rats were given a single tail‐vein injection of adeno‐associated virus carrying small hairpin RNAs (AAV‐shGAS5) and negative control (AAV‐NC). Four weeks after the injection, the expression of GAS5 in left ventricular myocardium was determined using qRT‐PCR. B, Myocardial submicroscopic structure was observed by transmission electron microscope (×5000). C, The effect of GAS5 knockdown on myocardial infarct size measured by TTC staining (n = 6 per group). The viable myocardium was stained red, and infarct myocardium was unstained. D,The effect of GAS5 knockdown on the changes of heart rate, left ventricular developed pressure (LVDP), positive first‐order derivative of ventricular pressure (+dp/dt) and negative first‐order derivative of ventricular pressure (−dp/dt). Baseline: cardiac function parameters were recorded 1 min before ischaemia. R‐30, R‐60 and R‐120: cardiac function parameters were collected after 30, 60 and 120 min of reperfusion (n = 6 per group). E, The effect of GAS5 knockdown on apoptosis measured by TUNEL staining (×400) (n = 6 per group). The apoptotic cell nuclei were stained brown, and the living cell nuclei were stained blue. F, The effect of GAS5 knockdown on cleaved caspase‐3 protein expression measured by immunohistochemistry (IHC) analysis (×400) (n = 6 per group). Data are expressed as the mean ± standard deviation (SD). ** P

    Techniques Used: Ex Vivo, Injection, Negative Control, Expressing, Quantitative RT-PCR, Transmission Assay, Microscopy, Staining, TUNEL Assay, Immunohistochemistry, Standard Deviation

    Effect of GAS5 knockdown on hypoxia/reoxygenation (H/R)‐induced cardiomyocyte injury in vitro. A, The expression of GAS5 in cardiomyocytes was measured by qRT‐PCR after transfected with GAS5‐specific small interfering RNA (siRNA), negative control (scramble). After 24 h of transfection, cells were subjected to 6 h of hypoxia, followed by 3 h of reoxygenation. Then, cell viability was measured by CCK‐8 assay (B). The lactate dehydrogenase (LDH) (C) and creatine kinase‐MB (CK‐MB) (D) activity in culture medium were measured by spectrophotometry. E, Cell apoptosis was detected by flow cytometry. F, Cleaved caspase‐3 protein expression was detected by Western blotting. Data are presented as the mean ± standard deviation, n = 3,* P
    Figure Legend Snippet: Effect of GAS5 knockdown on hypoxia/reoxygenation (H/R)‐induced cardiomyocyte injury in vitro. A, The expression of GAS5 in cardiomyocytes was measured by qRT‐PCR after transfected with GAS5‐specific small interfering RNA (siRNA), negative control (scramble). After 24 h of transfection, cells were subjected to 6 h of hypoxia, followed by 3 h of reoxygenation. Then, cell viability was measured by CCK‐8 assay (B). The lactate dehydrogenase (LDH) (C) and creatine kinase‐MB (CK‐MB) (D) activity in culture medium were measured by spectrophotometry. E, Cell apoptosis was detected by flow cytometry. F, Cleaved caspase‐3 protein expression was detected by Western blotting. Data are presented as the mean ± standard deviation, n = 3,* P

    Techniques Used: In Vitro, Expressing, Quantitative RT-PCR, Transfection, Small Interfering RNA, Negative Control, CCK-8 Assay, Activity Assay, Spectrophotometry, Flow Cytometry, Cytometry, Western Blot, Standard Deviation

    23) Product Images from "Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells"

    Article Title: Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2013/159864

    Htra2 induction and cleaved caspase-3 activation were detected by Western blot. Experiments were repeated three times, and the data were presented as mean ± SD (* P
    Figure Legend Snippet: Htra2 induction and cleaved caspase-3 activation were detected by Western blot. Experiments were repeated three times, and the data were presented as mean ± SD (* P

    Techniques Used: Activation Assay, Western Blot

    24) Product Images from "Ginkgo biloba Leaf Extract Protects against Myocardial Injury via Attenuation of Endoplasmic Reticulum Stress in Streptozotocin-Induced Diabetic ApoE−/− Mice"

    Article Title: Ginkgo biloba Leaf Extract Protects against Myocardial Injury via Attenuation of Endoplasmic Reticulum Stress in Streptozotocin-Induced Diabetic ApoE−/− Mice

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2018/2370617

    Effects of GBE on the cardiac histomorphological changes in diabetic ApoE −/− mice. (a) H E staining of cross-sectional tissue slices of the myocardium. (b) Masson's staining of collagen in the myocardium. The blue area against the red represents collagen deposition. Arrows indicate interstitial fibers. (c, d) Immunostaining of cleaved caspase-3. The brown-yellow area represents the positive expression of cleaved caspase-3. Images of the samples incubated only with the secondary antibody were provided correspondingly. (1A, 1B) Control group; (2A, 2B) untreated diabetic group; (3A, 3B) atorvastatin group; (4A, 4B) 200 mg/kg/day GBE group; and (5A, 5B) 400 mg/kg/day GBE group. (A) 100x magnification; (B) 200x magnification. ∗ P
    Figure Legend Snippet: Effects of GBE on the cardiac histomorphological changes in diabetic ApoE −/− mice. (a) H E staining of cross-sectional tissue slices of the myocardium. (b) Masson's staining of collagen in the myocardium. The blue area against the red represents collagen deposition. Arrows indicate interstitial fibers. (c, d) Immunostaining of cleaved caspase-3. The brown-yellow area represents the positive expression of cleaved caspase-3. Images of the samples incubated only with the secondary antibody were provided correspondingly. (1A, 1B) Control group; (2A, 2B) untreated diabetic group; (3A, 3B) atorvastatin group; (4A, 4B) 200 mg/kg/day GBE group; and (5A, 5B) 400 mg/kg/day GBE group. (A) 100x magnification; (B) 200x magnification. ∗ P

    Techniques Used: Mouse Assay, Staining, Immunostaining, Expressing, Incubation

    Effects of GBE on ERS-related apoptosis hallmark expression. Expression levels of p-JNK/JNK, CHOP, caspase-12, and cleaved caspase-3 were determined by Western blotting. The expression levels of CHOP, caspase-12, and cleaved caspase-3, were adjusted for GAPDH, and the expression of p-JNK was adjusted for total JNK. These values were normalized over the untreated diabetic group. (a, b) Expression levels of p-JNK/JNK; (c, d) expression of CHOP; (e, f) expression of caspase-12; and (g, h) expression of cleaved caspase-3. ∗ P
    Figure Legend Snippet: Effects of GBE on ERS-related apoptosis hallmark expression. Expression levels of p-JNK/JNK, CHOP, caspase-12, and cleaved caspase-3 were determined by Western blotting. The expression levels of CHOP, caspase-12, and cleaved caspase-3, were adjusted for GAPDH, and the expression of p-JNK was adjusted for total JNK. These values were normalized over the untreated diabetic group. (a, b) Expression levels of p-JNK/JNK; (c, d) expression of CHOP; (e, f) expression of caspase-12; and (g, h) expression of cleaved caspase-3. ∗ P

    Techniques Used: Expressing, Western Blot

    25) Product Images from "Upregulation of miR-335 ameliorates myocardial ischemia reperfusion injury via targeting hypoxia inducible factor 1-alpha subunit inhibitor"

    Article Title: Upregulation of miR-335 ameliorates myocardial ischemia reperfusion injury via targeting hypoxia inducible factor 1-alpha subunit inhibitor

    Journal: American Journal of Translational Research

    doi:

    The effect of microRNA (miR)-335 overexpression on mitochondrial permeability transition pore (MPTP) opening. H9c2 cells were transfected with miR-335 mimics or a negative control (mimics NC), or treated with 0.5 mM dimethyloxalylglycine (DMOG) for 24 h. A. MPTP opening was induced by CaCl 2 . The decrease in optical density (OD) reflected the extent of MPTP opening. B. Statistical analysis for MPTP opening. Minimum optical density (min OD) represents the OD value recorded at the onset of the experiment (0 min); maximum optical density (max OD) represents the OD value recorded at the end of the experiment (10 min). Min/max OD is negatively associated with the extent of MPTP opening. C. The protein expression of cytochrome c was measured by western blotting. D. The protein expression of cleaved caspase-9 was measured by western blotting. E. The protein expression of cleaved caspase-3 was measured by western blotting. Data are presented as the mean ± standard deviation from three independent experiments. * P
    Figure Legend Snippet: The effect of microRNA (miR)-335 overexpression on mitochondrial permeability transition pore (MPTP) opening. H9c2 cells were transfected with miR-335 mimics or a negative control (mimics NC), or treated with 0.5 mM dimethyloxalylglycine (DMOG) for 24 h. A. MPTP opening was induced by CaCl 2 . The decrease in optical density (OD) reflected the extent of MPTP opening. B. Statistical analysis for MPTP opening. Minimum optical density (min OD) represents the OD value recorded at the onset of the experiment (0 min); maximum optical density (max OD) represents the OD value recorded at the end of the experiment (10 min). Min/max OD is negatively associated with the extent of MPTP opening. C. The protein expression of cytochrome c was measured by western blotting. D. The protein expression of cleaved caspase-9 was measured by western blotting. E. The protein expression of cleaved caspase-3 was measured by western blotting. Data are presented as the mean ± standard deviation from three independent experiments. * P

    Techniques Used: Over Expression, Permeability, Transfection, Negative Control, Expressing, Western Blot, Standard Deviation

    26) Product Images from "Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas"

    Article Title: Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas

    Journal: Molecular Cancer

    doi: 10.1186/s12943-019-1120-1

    circ-MAPK4 behaved as an oncogene in glioma cells. ( a ) Circ-MAPK4 is also highly expressed in glioma cell lines, especially in U138 and U373, when compared to gial cell line (HA1800). ( b ) Schematic representation of target sequences of siRNAs which specially silence circ-MAPK4. ( c) The silence efficiency of siRNA targeting to circMAPK4 was analyzed by qPCR after transfected for 48 h in either U138 or U373 cells. ( d ) CCK-8 assays were performed to test survival of U138 and U373 cells after silencing of circ-MAPK4. ( e ) Colony formation assays were performed to test survival of U138 and U373 cells after silencing of circ-MAPK4. ( f ) Apoptosis assays were used to detect apoptosis levels of circ-MAPK4 silenced U138 and U373 cells. ( g ) Western blot assays were performed to analyze the protein expression levels of cleaved form of caspase-9, caspase-7, caspase-3, and PARP1 in circ-MAPK4 silenced U138 and U373 cells. ( h ) Transwell assays were carried out to test the invasive activity of circ-MAPK4 silenced U138 and U373 cells. The results above were summarized as bar graph. All data are the means ± SEM of three experiments. * P
    Figure Legend Snippet: circ-MAPK4 behaved as an oncogene in glioma cells. ( a ) Circ-MAPK4 is also highly expressed in glioma cell lines, especially in U138 and U373, when compared to gial cell line (HA1800). ( b ) Schematic representation of target sequences of siRNAs which specially silence circ-MAPK4. ( c) The silence efficiency of siRNA targeting to circMAPK4 was analyzed by qPCR after transfected for 48 h in either U138 or U373 cells. ( d ) CCK-8 assays were performed to test survival of U138 and U373 cells after silencing of circ-MAPK4. ( e ) Colony formation assays were performed to test survival of U138 and U373 cells after silencing of circ-MAPK4. ( f ) Apoptosis assays were used to detect apoptosis levels of circ-MAPK4 silenced U138 and U373 cells. ( g ) Western blot assays were performed to analyze the protein expression levels of cleaved form of caspase-9, caspase-7, caspase-3, and PARP1 in circ-MAPK4 silenced U138 and U373 cells. ( h ) Transwell assays were carried out to test the invasive activity of circ-MAPK4 silenced U138 and U373 cells. The results above were summarized as bar graph. All data are the means ± SEM of three experiments. * P

    Techniques Used: Real-time Polymerase Chain Reaction, Transfection, CCK-8 Assay, Western Blot, Expressing, Activity Assay

    Inhibition of phosphorylation of p38/MAPK reverses cell survival induced by circ-MAPK4. ( a ) U373 cells transfected with siRNA negative control, circ-MAPK4 siRNA-1 and siRNA-2 were treated with or without p-p38/MAPK inhibitor (SB203580). Inhibition efficiency of p-p38/MAPK inhibitor was accessed by testing phosphorylation and total protein levels of p38/MAPK and ERK using western blot assays. ( b , c ) CCK-8 and colony formation assays were performed to reveal cell survival of these U373 cells. ( d ) Apoptosis assays were performed to reveal apoptosis levels of these U373 cells. ( e ) The western blot assays were used to evaluate effect of p-p38/MAPK inhibitor on protein expression levels of cleaved form of caspase-9, caspase-7, caspase-3, PARP1 in these U373 cells. The data were summarized as bar graph. Data are the means ± SEM of three experiments. * P
    Figure Legend Snippet: Inhibition of phosphorylation of p38/MAPK reverses cell survival induced by circ-MAPK4. ( a ) U373 cells transfected with siRNA negative control, circ-MAPK4 siRNA-1 and siRNA-2 were treated with or without p-p38/MAPK inhibitor (SB203580). Inhibition efficiency of p-p38/MAPK inhibitor was accessed by testing phosphorylation and total protein levels of p38/MAPK and ERK using western blot assays. ( b , c ) CCK-8 and colony formation assays were performed to reveal cell survival of these U373 cells. ( d ) Apoptosis assays were performed to reveal apoptosis levels of these U373 cells. ( e ) The western blot assays were used to evaluate effect of p-p38/MAPK inhibitor on protein expression levels of cleaved form of caspase-9, caspase-7, caspase-3, PARP1 in these U373 cells. The data were summarized as bar graph. Data are the means ± SEM of three experiments. * P

    Techniques Used: Inhibition, Transfection, Negative Control, Western Blot, CCK-8 Assay, Expressing

    27) Product Images from "Circ‐AKT3 inhibits the accumulation of extracellular matrix of mesangial cells in diabetic nephropathy via modulating miR‐296‐3p/E‐cadherin signals, et al. Circ‐AKT3 inhibits the accumulation of extracellular matrix of mesangial cells in diabetic nephropathy via modulating miR‐296‐3p/E‐cadherin signals"

    Article Title: Circ‐AKT3 inhibits the accumulation of extracellular matrix of mesangial cells in diabetic nephropathy via modulating miR‐296‐3p/E‐cadherin signals, et al. Circ‐AKT3 inhibits the accumulation of extracellular matrix of mesangial cells in diabetic nephropathy via modulating miR‐296‐3p/E‐cadherin signals

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.15513

    Circ‐AKT3 overexpression inhibited the apoptosis of mouse mesangial SV40‐MES13 cells treated with high glucose. (A, B) Cell apoptosis of mouse mesangial SV40‐MES13 cells was detected by flow cytometry assay. (C) Bax, Bcl‐2, Caspase 3 and Cleaved caspase 3 protein expression were measured by Western blot analysis in each group. (D, E) Relative Bax/Bcl‐2 and Cleaved caspase 3/Caspase 3 protein expression were assessed by RT‐PCR in each group. * P
    Figure Legend Snippet: Circ‐AKT3 overexpression inhibited the apoptosis of mouse mesangial SV40‐MES13 cells treated with high glucose. (A, B) Cell apoptosis of mouse mesangial SV40‐MES13 cells was detected by flow cytometry assay. (C) Bax, Bcl‐2, Caspase 3 and Cleaved caspase 3 protein expression were measured by Western blot analysis in each group. (D, E) Relative Bax/Bcl‐2 and Cleaved caspase 3/Caspase 3 protein expression were assessed by RT‐PCR in each group. * P

    Techniques Used: Over Expression, Flow Cytometry, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

    Effect of circ‐AKT3/miR‐296‐3p/E‐cadherin on the apoptosis of mouse mesangial SV40‐MES13 cells. (A, B) Cell apoptosis of mouse mesangial SV40‐MES13 cells was detected by flow cytometry assay in each group. (C‐E) Relative Bax/Bcl‐2 and Cleaved caspase 3/Caspase 3 protein expression were measured via Western blot analysis in each group * P
    Figure Legend Snippet: Effect of circ‐AKT3/miR‐296‐3p/E‐cadherin on the apoptosis of mouse mesangial SV40‐MES13 cells. (A, B) Cell apoptosis of mouse mesangial SV40‐MES13 cells was detected by flow cytometry assay in each group. (C‐E) Relative Bax/Bcl‐2 and Cleaved caspase 3/Caspase 3 protein expression were measured via Western blot analysis in each group * P

    Techniques Used: Flow Cytometry, Expressing, Western Blot

    28) Product Images from "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress"

    Article Title: Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2017.347

    MARCH2 modulates ER stress. ( a ) Western blotting analysis of PERK, p-PERK (Thr 981) and p-EIF2 α (Ser 51), EIF2 α , ATF4, CHOP and ATG12-ATG5 levels in the indicated cell lines. ACTB was used as a loading control. ( b ) Control cells and Cas9- MARCH2 HCT116 cells were treated with BafA1 (10 nM) and/or DTT (1 mM) for 4 h and subjected western blotting to quantify CHOP and SQSTM1. ( c ) Control cells and Cas9- MARCH2 HCT116 cells were treated with or without salubrinal (5 μ M) for 2 h and subjected western blotting to quantify CHOP, LC3B-II, cleaved caspase-3, cleaved-PARP, CDC2, Cyclin B1 and MDM2. ( d ) Cells were transfected with siRNA control or siRNA targeting PPP1R3A for 24 h, and subjected Western blotting to quantify endogenous CHOP, LC3B-II, cleaved caspase-3, cleaved-PARP, CDC2, Cyclin B1 and MDM2
    Figure Legend Snippet: MARCH2 modulates ER stress. ( a ) Western blotting analysis of PERK, p-PERK (Thr 981) and p-EIF2 α (Ser 51), EIF2 α , ATF4, CHOP and ATG12-ATG5 levels in the indicated cell lines. ACTB was used as a loading control. ( b ) Control cells and Cas9- MARCH2 HCT116 cells were treated with BafA1 (10 nM) and/or DTT (1 mM) for 4 h and subjected western blotting to quantify CHOP and SQSTM1. ( c ) Control cells and Cas9- MARCH2 HCT116 cells were treated with or without salubrinal (5 μ M) for 2 h and subjected western blotting to quantify CHOP, LC3B-II, cleaved caspase-3, cleaved-PARP, CDC2, Cyclin B1 and MDM2. ( d ) Cells were transfected with siRNA control or siRNA targeting PPP1R3A for 24 h, and subjected Western blotting to quantify endogenous CHOP, LC3B-II, cleaved caspase-3, cleaved-PARP, CDC2, Cyclin B1 and MDM2

    Techniques Used: Western Blot, Transfection

    Knockout of MARCH2 in HCT116 cells promotes apoptosis. ( a ) Control cells and Cas9- MARCH2 HCT116 cells were serum-starved for 18 h, and then pulsed with 10% FCS for 24 h or 48 h with or without 50 μ m z-VAD-fmk. Apoptosis was measured by FITC–Annexin-V/PI staining and flow cytometry. ( b ) Data are mean±S.E.M. of at least three independent experiments. ( c ) Western blotting of the levels of cleaved caspase-3 and cleaved-PARP in Cas9- MARCH2 HCT116 cells treated as described in ( a ). ( d ) Quantification of cleaved caspase-3 and cleaved-PARP levels relative to ACTB in cells treated as described in ( c ). The average value in DMSO treated control cells for 24 h was normalized to 1. Data are mean±S.D. of three independent experiments. * P
    Figure Legend Snippet: Knockout of MARCH2 in HCT116 cells promotes apoptosis. ( a ) Control cells and Cas9- MARCH2 HCT116 cells were serum-starved for 18 h, and then pulsed with 10% FCS for 24 h or 48 h with or without 50 μ m z-VAD-fmk. Apoptosis was measured by FITC–Annexin-V/PI staining and flow cytometry. ( b ) Data are mean±S.E.M. of at least three independent experiments. ( c ) Western blotting of the levels of cleaved caspase-3 and cleaved-PARP in Cas9- MARCH2 HCT116 cells treated as described in ( a ). ( d ) Quantification of cleaved caspase-3 and cleaved-PARP levels relative to ACTB in cells treated as described in ( c ). The average value in DMSO treated control cells for 24 h was normalized to 1. Data are mean±S.D. of three independent experiments. * P

    Techniques Used: Knock-Out, Staining, Flow Cytometry, Cytometry, Western Blot

    MARCH2 promotes the tumorigenicity of colon cancer cells in vivo . ( a ) Empty vector-transfected HCT116 cells, MARCH2-overexpressing HCT116 cells, control (wild-type) HCT116 cells or Cas9- MARCH2 HCT116 cells were subcutaneously injected into BALB/c nude mice ( n =6). Xenograft tumors were excised and imaged on day 20. Scale bar: 1 cm. ( b ) Immunohistochemical staining for MARCH2, Ki-67, SQSTM1 and cleaved caspase-3 in xenograft tumor tissues. ( c ) The number of positive cells per 200 cells in ( b ). ** P
    Figure Legend Snippet: MARCH2 promotes the tumorigenicity of colon cancer cells in vivo . ( a ) Empty vector-transfected HCT116 cells, MARCH2-overexpressing HCT116 cells, control (wild-type) HCT116 cells or Cas9- MARCH2 HCT116 cells were subcutaneously injected into BALB/c nude mice ( n =6). Xenograft tumors were excised and imaged on day 20. Scale bar: 1 cm. ( b ) Immunohistochemical staining for MARCH2, Ki-67, SQSTM1 and cleaved caspase-3 in xenograft tumor tissues. ( c ) The number of positive cells per 200 cells in ( b ). ** P

    Techniques Used: In Vivo, Plasmid Preparation, Transfection, Injection, Mouse Assay, Immunohistochemistry, Staining

    29) Product Images from "Circular RNA hsa_circ_0003141 promotes tumorigenesis of hepatocellular carcinoma via a miR-1827/UBAP2 axis"

    Article Title: Circular RNA hsa_circ_0003141 promotes tumorigenesis of hepatocellular carcinoma via a miR-1827/UBAP2 axis

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.103244

    Knockdown of hsa_circ_0003141 inhibits tumorigenesis of Huh-7 subcutaneous xenografts in vivo . Mice were s.c. implanted with hsa_circ_0003141-shRNA1-transfected Huh-7 cells. ( A ) Xenograft tumor volume monitored weekly. ( B , C ) Xenografts tumors were photographed and calculated. ( D ) Expression of UBAP2 and cleaved caspase 3 in tumor tissues analyzed by western blotting. ( E , F ) The relative expression of UBAP2 and cleaved caspase 3 in tumor tissues normalized to β-actin. **P
    Figure Legend Snippet: Knockdown of hsa_circ_0003141 inhibits tumorigenesis of Huh-7 subcutaneous xenografts in vivo . Mice were s.c. implanted with hsa_circ_0003141-shRNA1-transfected Huh-7 cells. ( A ) Xenograft tumor volume monitored weekly. ( B , C ) Xenografts tumors were photographed and calculated. ( D ) Expression of UBAP2 and cleaved caspase 3 in tumor tissues analyzed by western blotting. ( E , F ) The relative expression of UBAP2 and cleaved caspase 3 in tumor tissues normalized to β-actin. **P

    Techniques Used: In Vivo, Mouse Assay, Transfection, Expressing, Western Blot

    Hsa_circ_0003141 functions as oncogene in Hun-7 cells by sponging miR-1827, and thus increasing UBAP2 expression. ( A ) Western analysis of UBAP2 and cleaved caspase 3 in Huh-7 cells transfected with hsa_circ_0003141-shRNA1 with and without miR-1827 inhibitor. ( B , C ) The relative expression of UBAP2 and cleaved caspase 3 in Huh-7 cells normalized to β-actin. **P
    Figure Legend Snippet: Hsa_circ_0003141 functions as oncogene in Hun-7 cells by sponging miR-1827, and thus increasing UBAP2 expression. ( A ) Western analysis of UBAP2 and cleaved caspase 3 in Huh-7 cells transfected with hsa_circ_0003141-shRNA1 with and without miR-1827 inhibitor. ( B , C ) The relative expression of UBAP2 and cleaved caspase 3 in Huh-7 cells normalized to β-actin. **P

    Techniques Used: Expressing, Western Blot, Transfection

    30) Product Images from "Medicinal supplement genipin induces p53 and Bax-dependent apoptosis in colon cancer cells"

    Article Title: Medicinal supplement genipin induces p53 and Bax-dependent apoptosis in colon cancer cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.9025

    Effect of genipin on the expression of apoptosis-associated proteins in colon cancer cells. (A) The expression levels of apoptosis-associated proteins in HCT116 cells treated with genipin (0, 200, 400 and 600 µM) for 24 h were analyzed by western blotting using anti-p53, anti-Bcl-2, anti-Bax and anti-cleaved caspase-3 antibodies. (B) The results of the western blot analysis were quantified; n=5. Data are expressed as the mean ± standard deviation. *P
    Figure Legend Snippet: Effect of genipin on the expression of apoptosis-associated proteins in colon cancer cells. (A) The expression levels of apoptosis-associated proteins in HCT116 cells treated with genipin (0, 200, 400 and 600 µM) for 24 h were analyzed by western blotting using anti-p53, anti-Bcl-2, anti-Bax and anti-cleaved caspase-3 antibodies. (B) The results of the western blot analysis were quantified; n=5. Data are expressed as the mean ± standard deviation. *P

    Techniques Used: Expressing, Western Blot, Standard Deviation

    31) Product Images from "Overexpression of retinoblastoma-binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression"

    Article Title: Overexpression of retinoblastoma-binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2018.9153

    RbAp48 in combination with radiation regulates the expression of apoptosis-associated proteins. (A) Reverse transcription-quantitative polymerase chain reaction was performed to assess the expression levels of caspase-3, −7, −9, PARP, Bcl-2 and Bax in AGS cells. (B) Western blot analysis was performed to evaluate the expression levels of cleaved caspase-3, −7, −9 and cleaved PARP. *P
    Figure Legend Snippet: RbAp48 in combination with radiation regulates the expression of apoptosis-associated proteins. (A) Reverse transcription-quantitative polymerase chain reaction was performed to assess the expression levels of caspase-3, −7, −9, PARP, Bcl-2 and Bax in AGS cells. (B) Western blot analysis was performed to evaluate the expression levels of cleaved caspase-3, −7, −9 and cleaved PARP. *P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    32) Product Images from "Silencing of Annexin A1 suppressed the apoptosis and inflammatory response of preeclampsia rat trophoblasts"

    Article Title: Silencing of Annexin A1 suppressed the apoptosis and inflammatory response of preeclampsia rat trophoblasts

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3887

    Knockdown of ANXA1 regulated the apoptosis-associated factors in trophoblasts. (A) Bax and (B) Bcl-2 mRNA expression levels were detected using reverse transcription-quantitative polymerase chain reaction. (C) Protein expression levels of pro-caspase-3, cleaved-caspase-3, Bax and Bcl-2 were measured using western blot analysis. ** P
    Figure Legend Snippet: Knockdown of ANXA1 regulated the apoptosis-associated factors in trophoblasts. (A) Bax and (B) Bcl-2 mRNA expression levels were detected using reverse transcription-quantitative polymerase chain reaction. (C) Protein expression levels of pro-caspase-3, cleaved-caspase-3, Bax and Bcl-2 were measured using western blot analysis. ** P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    33) Product Images from "Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma"

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.906616

    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Figure Legend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Techniques Used: Expressing, Cotransfection

    34) Product Images from "Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes"

    Article Title: Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2018.6388

    Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved caspase-3, total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P
    Figure Legend Snippet: Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved caspase-3, total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P

    Techniques Used: Fluorescence, Expressing, Western Blot

    35) Product Images from "Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation"

    Article Title: Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation

    Journal: Toxins

    doi: 10.3390/toxins10060235

    Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved caspase-3 in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p
    Figure Legend Snippet: Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved caspase-3 in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p

    Techniques Used: Expressing, Cell Culture, Western Blot

    Proposed model for MC-LR-induced testis germ cell apoptosis in rats via SIRT1 signaling pathway activation. MC-LR exposure downregulated SIRT1 levels in primary co-cultured Sertoli–germ cells and rat testes, subsequently increasing p53 and Ku70 acetylation, and Bax/Bcl-2 and cleaved caspase-3 expression. RES pretreatment could promote the activation of SIRT1, which could resist MC-LR-induced apoptosis via reducing the acetylation of p53 and Ku70, as well as Bax/Bcl-2 and caspase-3 activation.
    Figure Legend Snippet: Proposed model for MC-LR-induced testis germ cell apoptosis in rats via SIRT1 signaling pathway activation. MC-LR exposure downregulated SIRT1 levels in primary co-cultured Sertoli–germ cells and rat testes, subsequently increasing p53 and Ku70 acetylation, and Bax/Bcl-2 and cleaved caspase-3 expression. RES pretreatment could promote the activation of SIRT1, which could resist MC-LR-induced apoptosis via reducing the acetylation of p53 and Ku70, as well as Bax/Bcl-2 and caspase-3 activation.

    Techniques Used: Activation Assay, Cell Culture, Expressing

    36) Product Images from "Lin28A activates androgen receptor via regulation of c-myc and promotes malignancy of ER−/Her2+ breast cancer"

    Article Title: Lin28A activates androgen receptor via regulation of c-myc and promotes malignancy of ER−/Her2+ breast cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.11004

    Lin28A played a critical role in the G1/S progression and apoptosis in ER-/Her2+ breast cancer cells A. The flow cytometry analysis showed that the number of MDA-MB-453 cells in G1 phase increased, while the number of cells in S phase decreased in the Lin28A siRNA#2 group compared to the control group. Western blot analysis of a series of cell cycle-related molecules, including PCNA and cyclin E were detected. B. The number of SK-BR-3 cells in G1 phase decreased, while the number of cells in S phase increased in the Lin28A group compared to the control group. Western blot analysis of a series of cell cycle-related molecules, including PCNA and cyclin E were detected. C. The percentage of apoptotic cells was higher in the Lin28A siRNA#2 group compared to the control group in MDA-MB-453 cells. Western blotting was used to analyse the levels of the anti-apoptosis marker Bcl-2, pro-apoptotic marker procaspase-3 and cleaved caspase-3. D. The percentage of apoptotic cells was lower in the Lin28A group compared to the control group in SK-BR-3 cells. Western blotting was used to analyse the levels of the anti-apoptosis marker Bcl-2, pro-apoptotic marker procaspase-3 and cleaved caspase-3.
    Figure Legend Snippet: Lin28A played a critical role in the G1/S progression and apoptosis in ER-/Her2+ breast cancer cells A. The flow cytometry analysis showed that the number of MDA-MB-453 cells in G1 phase increased, while the number of cells in S phase decreased in the Lin28A siRNA#2 group compared to the control group. Western blot analysis of a series of cell cycle-related molecules, including PCNA and cyclin E were detected. B. The number of SK-BR-3 cells in G1 phase decreased, while the number of cells in S phase increased in the Lin28A group compared to the control group. Western blot analysis of a series of cell cycle-related molecules, including PCNA and cyclin E were detected. C. The percentage of apoptotic cells was higher in the Lin28A siRNA#2 group compared to the control group in MDA-MB-453 cells. Western blotting was used to analyse the levels of the anti-apoptosis marker Bcl-2, pro-apoptotic marker procaspase-3 and cleaved caspase-3. D. The percentage of apoptotic cells was lower in the Lin28A group compared to the control group in SK-BR-3 cells. Western blotting was used to analyse the levels of the anti-apoptosis marker Bcl-2, pro-apoptotic marker procaspase-3 and cleaved caspase-3.

    Techniques Used: Flow Cytometry, Cytometry, Multiple Displacement Amplification, Western Blot, Marker

    37) Product Images from "Neuroprotective Effects of Ginsenoside-Rg1 Against Depression-Like Behaviors via Suppressing Glial Activation, Synaptic Deficits, and Neuronal Apoptosis in Rats"

    Article Title: Neuroprotective Effects of Ginsenoside-Rg1 Against Depression-Like Behaviors via Suppressing Glial Activation, Synaptic Deficits, and Neuronal Apoptosis in Rats

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02889

    Ginsenoside-Rg1 suppressed the expression of apoptosis-related proteins within the vmPFC region as induced by CUMS-exposure. (A) Expression of cleaved caspase-3, caspase-9, and Bcl-2 within with the vmPFC region with use of immunohistochemical staining. Scale bar is 20 μm. (B) Bar graphs show quantification of cleaved caspase-3, caspase-9, and Bcl-2. Values are expressed relative to those of the control group. (C) Expression of cleaved caspase-3, caspase-9, and Bcl-2 within with the vmPFC region with use of Western Blot assays. (D) Expression of phosphorylated p38, p65, and Nrf2 within with the vmPFC region with use of Western Blot assays. N = 6 per group. Data were presented as the means ± SEM. * P
    Figure Legend Snippet: Ginsenoside-Rg1 suppressed the expression of apoptosis-related proteins within the vmPFC region as induced by CUMS-exposure. (A) Expression of cleaved caspase-3, caspase-9, and Bcl-2 within with the vmPFC region with use of immunohistochemical staining. Scale bar is 20 μm. (B) Bar graphs show quantification of cleaved caspase-3, caspase-9, and Bcl-2. Values are expressed relative to those of the control group. (C) Expression of cleaved caspase-3, caspase-9, and Bcl-2 within with the vmPFC region with use of Western Blot assays. (D) Expression of phosphorylated p38, p65, and Nrf2 within with the vmPFC region with use of Western Blot assays. N = 6 per group. Data were presented as the means ± SEM. * P

    Techniques Used: Expressing, Immunohistochemistry, Staining, Western Blot

    Ginsenoside-Rg1 reduced neuronal apoptosis within the vmPFC region as induced by CUMS-exposure.  (A)  Representative images of TUNEL staining from the vmPFC region. Scale bar is 20 μm.  N  = 4 per group.  (B)  Representative images of immunohistochemical staining of NeuN expression within the vmPFC region. Scale bar is 20 μm.  N  = 4 per group.  (C)  Representative images of immunofluorescence staining of NeuN/cleaved Caspase 3 double labeled positive cells within the vmPFC region. Scale bar is 20 μm.  N  = 4 per group.  (D)  Representative electron micrograph of vmPFC neuronal ultrastructure revealing nuclear chromatin aggregation, condensation and margination. Scale bar is 1 μm.  (E)  Bar graphs show quantification of TUNEL positive cells density in the vmPFC region.  (F)  Bar graphs show quantification of NeuN positive cells density in the vmPFC region.  (G)  Bar graphs show quantification of NeuN/cleaved Caspase 3 positive cells in the vmPFC region.  N  = 6 per group. Data were presented as the means ± SEM.  ** P
    Figure Legend Snippet: Ginsenoside-Rg1 reduced neuronal apoptosis within the vmPFC region as induced by CUMS-exposure. (A) Representative images of TUNEL staining from the vmPFC region. Scale bar is 20 μm. N = 4 per group. (B) Representative images of immunohistochemical staining of NeuN expression within the vmPFC region. Scale bar is 20 μm. N = 4 per group. (C) Representative images of immunofluorescence staining of NeuN/cleaved Caspase 3 double labeled positive cells within the vmPFC region. Scale bar is 20 μm. N = 4 per group. (D) Representative electron micrograph of vmPFC neuronal ultrastructure revealing nuclear chromatin aggregation, condensation and margination. Scale bar is 1 μm. (E) Bar graphs show quantification of TUNEL positive cells density in the vmPFC region. (F) Bar graphs show quantification of NeuN positive cells density in the vmPFC region. (G) Bar graphs show quantification of NeuN/cleaved Caspase 3 positive cells in the vmPFC region. N = 6 per group. Data were presented as the means ± SEM. ** P

    Techniques Used: TUNEL Assay, Staining, Immunohistochemistry, Expressing, Immunofluorescence, Labeling

    38) Product Images from "MicroRNA-21 Overexpression Promotes the Neuroprotective Efficacy of Mesenchymal Stem Cells for Treatment of Intracerebral Hemorrhage"

    Article Title: MicroRNA-21 Overexpression Promotes the Neuroprotective Efficacy of Mesenchymal Stem Cells for Treatment of Intracerebral Hemorrhage

    Journal: Frontiers in Neurology

    doi: 10.3389/fneur.2018.00931

    miR-21 can reduce PC12 cell death and TRPM7 is a functional target of miR-21 (A) . MiR-21 transfection efficiency detected by qPCR (B) . Apoptosis rate of PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (C) . Fluo-4 assay of PC12 cells (D) . The expression level of TRPM7 in PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (E) . Target region of miR-21 and TRPM7 (F) . Luciferase assay for the combination of miR-21 and TRPM7 (G) . Western blot analyzing the expression level of TRPM7, p65, and p-IκB proteins (H) . Quantitative histogram of TRPM7 expression level in different groups (I) . Western blot analyzing the expression level of cleaved caspase-3. * p
    Figure Legend Snippet: miR-21 can reduce PC12 cell death and TRPM7 is a functional target of miR-21 (A) . MiR-21 transfection efficiency detected by qPCR (B) . Apoptosis rate of PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (C) . Fluo-4 assay of PC12 cells (D) . The expression level of TRPM7 in PC12 cells transfected with miR-21 mimics, miR-21 inhibitors, and their corresponding negative controls (NC) (E) . Target region of miR-21 and TRPM7 (F) . Luciferase assay for the combination of miR-21 and TRPM7 (G) . Western blot analyzing the expression level of TRPM7, p65, and p-IκB proteins (H) . Quantitative histogram of TRPM7 expression level in different groups (I) . Western blot analyzing the expression level of cleaved caspase-3. * p

    Techniques Used: Functional Assay, Transfection, Real-time Polymerase Chain Reaction, Expressing, Luciferase, Western Blot

    miR-21 enhanced survival of Hemin-induced mesenchymal stem cells (MSCs) (A) . Viability of MSCs treated with Hemin for different lengths of time (B) . Expression level of miR-21 detected by qPCR (C) . Viability of MSCs transfected with miR-21 mimics, miR-21 inhibitors, or their corresponding negative control (NC) (D) . Cell apoptosis analyzed by Annexin V-FITC and PI staining of MSCs in different groups (E) . Western blot analysis of cleaved-caspase-3 expression level in MSCs transfected with different miRNAs. * p
    Figure Legend Snippet: miR-21 enhanced survival of Hemin-induced mesenchymal stem cells (MSCs) (A) . Viability of MSCs treated with Hemin for different lengths of time (B) . Expression level of miR-21 detected by qPCR (C) . Viability of MSCs transfected with miR-21 mimics, miR-21 inhibitors, or their corresponding negative control (NC) (D) . Cell apoptosis analyzed by Annexin V-FITC and PI staining of MSCs in different groups (E) . Western blot analysis of cleaved-caspase-3 expression level in MSCs transfected with different miRNAs. * p

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Transfection, Negative Control, Staining, Western Blot

    39) Product Images from "Seawater inhalation induces acute lung injury via ROS generation and the endoplasmic reticulum stress pathway"

    Article Title: Seawater inhalation induces acute lung injury via ROS generation and the endoplasmic reticulum stress pathway

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3486

    SW-induced cell apoptosis and growth inhibition are dependent on endoplasmic reticulum stress. (A) Effects of 4-PBA (30 mg/kg) on SW-induced apoptosis in rats (TUNEL staining). (a) Control, (b) SW, (c) 4-PBA and (d) 4-PBA + SW groups (magnification, ×10). (B) Effects of 4-PBA (2 mM) or Thap (150 nM) on SW-induced apoptosis of A549 cells (Annexin V-FITC/PI staining). (a) Control, (b) SW, (c) 4-PBA, (d) 4-PBA + SW and (e) Thap groups. (C) Number of apoptotic cells per field in (A). (D) Percentage of apoptotic cells in (B). (E-G) Effects of 4-PBA or Thap on the expression of apoptosis-associated proteins caspase-3 and p-JNK. (H) Effects of 4-PBA or Thap on SW-induced cell growth inhibition (Cell Counting kit-8 assay). Data are presented as the means ± standard error of the mean, n=5. * P
    Figure Legend Snippet: SW-induced cell apoptosis and growth inhibition are dependent on endoplasmic reticulum stress. (A) Effects of 4-PBA (30 mg/kg) on SW-induced apoptosis in rats (TUNEL staining). (a) Control, (b) SW, (c) 4-PBA and (d) 4-PBA + SW groups (magnification, ×10). (B) Effects of 4-PBA (2 mM) or Thap (150 nM) on SW-induced apoptosis of A549 cells (Annexin V-FITC/PI staining). (a) Control, (b) SW, (c) 4-PBA, (d) 4-PBA + SW and (e) Thap groups. (C) Number of apoptotic cells per field in (A). (D) Percentage of apoptotic cells in (B). (E-G) Effects of 4-PBA or Thap on the expression of apoptosis-associated proteins caspase-3 and p-JNK. (H) Effects of 4-PBA or Thap on SW-induced cell growth inhibition (Cell Counting kit-8 assay). Data are presented as the means ± standard error of the mean, n=5. * P

    Techniques Used: Inhibition, TUNEL Assay, Staining, Expressing, Cell Counting

    SW-induced growth inhibition and apoptosis depend on ROS. (A) Effects of NAC (150 mg/kg) pretreatment on SW-induced apoptosis in rats (TUNEL staining; magnification, ×10). (B) Effects of NAC (5 mM) on SW-induced apoptosis of A549 cells (Annexin V-FITC/PI staining). (a) Control, (b) SW, (c) NAC and (d) NAC + SW groups. (C) Number of apoptotic cells per field in (A). (D) Percentage of apoptotic cells in (B). (E-G) Effects of NAC (5 mM) on the expression of the apoptosis-associated proteins caspase-3 and p-JNK in A549 cells. (H) Effects of NAC on SW-induced cell growth inhibition (Cell Counting kit-8 assay). Data are presented as the means ± standard error of the mean, n=5. *** P
    Figure Legend Snippet: SW-induced growth inhibition and apoptosis depend on ROS. (A) Effects of NAC (150 mg/kg) pretreatment on SW-induced apoptosis in rats (TUNEL staining; magnification, ×10). (B) Effects of NAC (5 mM) on SW-induced apoptosis of A549 cells (Annexin V-FITC/PI staining). (a) Control, (b) SW, (c) NAC and (d) NAC + SW groups. (C) Number of apoptotic cells per field in (A). (D) Percentage of apoptotic cells in (B). (E-G) Effects of NAC (5 mM) on the expression of the apoptosis-associated proteins caspase-3 and p-JNK in A549 cells. (H) Effects of NAC on SW-induced cell growth inhibition (Cell Counting kit-8 assay). Data are presented as the means ± standard error of the mean, n=5. *** P

    Techniques Used: Inhibition, TUNEL Assay, Staining, Expressing, Cell Counting

    40) Product Images from "Nicorandil pretreatment inhibits myocardial apoptosis and improves cardiac function after coronary microembolization in rats"

    Article Title: Nicorandil pretreatment inhibits myocardial apoptosis and improves cardiac function after coronary microembolization in rats

    Journal: Journal of Geriatric Cardiology : JGC

    doi: 10.11909/j.issn.1671-5411.2018.09.002

    Cleaved caspase-3 protein expression detected by Western blot. The data were obtained from at least three independent experiments, and the values represent the mean ± SD. a P
    Figure Legend Snippet: Cleaved caspase-3 protein expression detected by Western blot. The data were obtained from at least three independent experiments, and the values represent the mean ± SD. a P

    Techniques Used: Expressing, Western Blot

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    Incubation:

    Article Title: Roles of autophagy in androgen-induced benign prostatic hyperplasia in castrated rats
    Article Snippet: .. Membranes were blocked with TBS supplemented with 0.1% Tween-20 (v/v) and 5% (w/v) non-fat dry milk for 30 min at room temperature prior to incubation with the following primary antibodies for 12 h: Anti-LC3 (cat no. 3868; Cell Signaling Technology, Danvers, MA, USA; 1:1,000); anti-Beclin-1 (cat no. ab62557; Abcam; Cambridge, MA, USA, 1:1,000) and anti-caspase-3 (cat no. ab32351; Abcam; 1:1,000). ..

    Article Title: MicroRNA-21 Overexpression Promotes the Neuroprotective Efficacy of Mesenchymal Stem Cells for Treatment of Intracerebral Hemorrhage
    Article Snippet: .. The membranes were incubated in 1% BSA solution with primary antibodies including anti-cleaved caspase-3, anti-matrix metalloproteinase 2 (MMP2), anti-matrix metalloproteinase 9 (MMP9), anti-tissue inhibitor of metalloproteinase-1 (TIMP-1)TIMP1, anti-transient receptor potential melastatin 7 (TRPM7), anti-p65, anti-phospho-IkB, and anti-beta actin (Abcam, CA, United States) at 4°C overnight and then incubated with the secondary antibody for 1 h at 22–25°C. .. The protein bands were detected using ECL (Electro-Chemi-Luminescence) substrate with the chemiluminescence method (LAS-3000, FUJIFILM, Japan).

    Article Title: Farnesyltransferase inhibitor R115777 inhibits cell growth and induces apoptosis in mantle cell lymphoma
    Article Snippet: .. Slides were incubated 1 hour with the active caspase-3 antibody diluted (1/10) in blocking solution (ab2302, Abcam). .. After washes, slides were incubated for 1h with secondary antibody (A-31572, Molecular Probes), stained in DAPI diluted 1/5000 in VECTASHIELD and viewed under the microscope.

    Article Title: Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone
    Article Snippet: .. The membrane was incubated at room temperature in blocking solution (5% nonfat milk) for 1 h followed by incubation for 2 h in blocking solution containing an appropriate dilution of anti- Caspase-3, Caspase-8, Caspase-9, Bcl-2, Bax, Bid, Bad, Drp1, Opa1, Mfn1, Mfn2 antibody (Abcam, UK). .. After washing, blots were then probed with appropriate secondary horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA) and detected by an ECL detection system (Millipore) and scanned by MultiGel-21 (Top Bio, Taiwan). β-actin served as internal control.

    Blocking Assay:

    Article Title: Farnesyltransferase inhibitor R115777 inhibits cell growth and induces apoptosis in mantle cell lymphoma
    Article Snippet: .. Slides were incubated 1 hour with the active caspase-3 antibody diluted (1/10) in blocking solution (ab2302, Abcam). .. After washes, slides were incubated for 1h with secondary antibody (A-31572, Molecular Probes), stained in DAPI diluted 1/5000 in VECTASHIELD and viewed under the microscope.

    Article Title: Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone
    Article Snippet: .. The membrane was incubated at room temperature in blocking solution (5% nonfat milk) for 1 h followed by incubation for 2 h in blocking solution containing an appropriate dilution of anti- Caspase-3, Caspase-8, Caspase-9, Bcl-2, Bax, Bid, Bad, Drp1, Opa1, Mfn1, Mfn2 antibody (Abcam, UK). .. After washing, blots were then probed with appropriate secondary horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA) and detected by an ECL detection system (Millipore) and scanned by MultiGel-21 (Top Bio, Taiwan). β-actin served as internal control.

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    Abcam anti cleaved caspase 3
    Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved <t>caspase-3</t> in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p
    Anti Cleaved Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3/product/Abcam
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    99
    Abcam rabbit anti caspase 3
    Inhibition of ER stress-related apoptosis in the sciatic nerve of DPN rats by intrathecal injection of IRE1α siRNA. ( A ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against P-IRE1α and XBP-1s. β-actin was probed as loading control. The intensity is expressed as mean ± SEM of the percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis or unpaired Student’s t-test. ( B ) TUNEL, p-JNK, and Caspae-12 were effectively eliminated as measured by immunohistochemistry method. Data are expressed as mean ± SEM of the percentage of the respective controls and analyzed using one-way ANOVA with Tamhane’s T2 analysis or unpaired Student’s t-test. ( C ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against GRP78, CHOP, Bcl-2, Bax and <t>Cleaved-Caspase-3.</t> β-actin was probed as loading control. Results of are expressed as mean ± SEM indicated as percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis (GRP78, Cleaved-Caspase-3, CHOP) or Tamhane’s T2 analysis (Bcl-2, Bax) or unpaired Student’s t-test. Δ P
    Rabbit Anti Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti caspase 3/product/Abcam
    Average 99 stars, based on 28 article reviews
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    Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved caspase-3 in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p

    Journal: Toxins

    Article Title: Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation

    doi: 10.3390/toxins10060235

    Figure Lengend Snippet: Apoptosis-related protein expression levels in co-cultured Sertoli–germ cells and testicular tissues exposed to MC-LR in the presence or absence of RES. ( A – D ) Western blot of sirtuin 1 (SIRT1), p53, acetylated p53, Bax, Bcl-2, and cleaved caspase-3 in co-cultured Sertoli–germ cells exposed to MC-LR in the presence or absence of RES; and ( E , F ) Western blot of apoptosis-related protein expression levels in testicular tissues of rats exposed to MC-LR with or without RES. The expression levels were quantified with Quantity One. β-actin was used as a loading control. Data are presented as mean ± SEM for each group; * p

    Article Snippet: The membranes were blocked in tris buffered saline with tween (TBST) containing 5% BSA at 23 °C for two hours, and immunoblotted using primary anti-Ku70 (sc-17789, Santa Cruz Biotechnology, Boston, CA, USA), anti-SIRT1 (ab110304), anti-p53 (ab131442), anti-p53 (acetyl K381, ab61241), anti-cleaved-caspase-3 (ab2302), anti-Bax (ab32503), anti-Bcl-2 (ab7973), and anti-β-actin (ab6276) (Abcam, Cambridge, UK).

    Techniques: Expressing, Cell Culture, Western Blot

    FOXK2 expression affects c-caspase-3 expression and BGC-823 cell apoptosis. (A) si-FOXK2 transfection effectively reduced FOXK2 mRNA expression in BGC-823 cells, as well as (B) FOXK2 and c-caspase-3 protein expression. (C) FOXK2 overexpression plasmid transfection successfully increased FOXK2 mRNA. (D) FOXK2 and c-caspase-3 protein expression also increased. (E) si-FOXK2 decreased BGC-823 cell apoptosis, whereas (F) FOXK2 overexpression increased BGC-823 cell apoptosis. *P

    Journal: Molecular Medicine Reports

    Article Title: Downregulation of FOXK2 is associated with poor prognosis in patients with gastric cancer

    doi: 10.3892/mmr.2018.9466

    Figure Lengend Snippet: FOXK2 expression affects c-caspase-3 expression and BGC-823 cell apoptosis. (A) si-FOXK2 transfection effectively reduced FOXK2 mRNA expression in BGC-823 cells, as well as (B) FOXK2 and c-caspase-3 protein expression. (C) FOXK2 overexpression plasmid transfection successfully increased FOXK2 mRNA. (D) FOXK2 and c-caspase-3 protein expression also increased. (E) si-FOXK2 decreased BGC-823 cell apoptosis, whereas (F) FOXK2 overexpression increased BGC-823 cell apoptosis. *P

    Article Snippet: FOXK2 (cat. no. ab50946; Abcam), cleaved caspase-3 (cat. no. ab2302; Abcam), E-cadherin (cat. no. ab15148; Abcam) and N-cadherin (cat. no. ab18203; Abcam) antibodies were purchased from Abcam.

    Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation

    SGO1 promotes tumor formation in nude mice model. PC3 cells (A) and DU145 cells (D) were infected with the lentivirus containing SGO1-shRNA-B respectively, and the cells were implanted subcutaneously in nude mice, and the tumor-bearing nude mice were photographed three weeks later. (B, E) Tumor volume of PC3 cells and DU145 cells were recorded during tumor growth respectively. (C, F) At the end of the experiment, the tumor weight of each group was measured. (G) After embedding the above-described tumor tissues in paraffin, the expression of Ki67 and cleaved caspase 3 was detected by immunohistochemistry.

    Journal: American Journal of Cancer Research

    Article Title: SGO1 induces proliferation and metastasis of prostate cancer through AKT-mediated signaling pathway

    doi:

    Figure Lengend Snippet: SGO1 promotes tumor formation in nude mice model. PC3 cells (A) and DU145 cells (D) were infected with the lentivirus containing SGO1-shRNA-B respectively, and the cells were implanted subcutaneously in nude mice, and the tumor-bearing nude mice were photographed three weeks later. (B, E) Tumor volume of PC3 cells and DU145 cells were recorded during tumor growth respectively. (C, F) At the end of the experiment, the tumor weight of each group was measured. (G) After embedding the above-described tumor tissues in paraffin, the expression of Ki67 and cleaved caspase 3 was detected by immunohistochemistry.

    Article Snippet: The antibodies purchased include SGO1 (Abcam, ab58023), GAPDH (Cell Signaling Technology, 2118), cyclin A2 (Cell Signaling Technology, 4656), CDK2 (Cell Signaling Technology, 2546), cyclin D1 (Cell Signaling Technology, 4978), AKT (Cell Signaling Technology, 4685), pAKT Ser473 (Cell Signaling Technology, 4060), cleaved caspase-3 (Abcam, ab2302), cleaved caspase-9 (Abcam, ab2324), caspase-3 (Abcam, ab13847), caspase-9 (Abcam, ab32539), cleaved PARP1 (Abeam, ab32064), PARP (Abeam, ab74290), pBad Ser136 (Cell Signaling Technology, 4336).

    Techniques: Mouse Assay, Infection, shRNA, Expressing, Immunohistochemistry

    Inhibition of ER stress-related apoptosis in the sciatic nerve of DPN rats by intrathecal injection of IRE1α siRNA. ( A ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against P-IRE1α and XBP-1s. β-actin was probed as loading control. The intensity is expressed as mean ± SEM of the percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis or unpaired Student’s t-test. ( B ) TUNEL, p-JNK, and Caspae-12 were effectively eliminated as measured by immunohistochemistry method. Data are expressed as mean ± SEM of the percentage of the respective controls and analyzed using one-way ANOVA with Tamhane’s T2 analysis or unpaired Student’s t-test. ( C ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against GRP78, CHOP, Bcl-2, Bax and Cleaved-Caspase-3. β-actin was probed as loading control. Results of are expressed as mean ± SEM indicated as percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis (GRP78, Cleaved-Caspase-3, CHOP) or Tamhane’s T2 analysis (Bcl-2, Bax) or unpaired Student’s t-test. Δ P

    Journal: Scientific Reports

    Article Title: IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro

    doi: 10.1038/s41598-018-20950-9

    Figure Lengend Snippet: Inhibition of ER stress-related apoptosis in the sciatic nerve of DPN rats by intrathecal injection of IRE1α siRNA. ( A ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against P-IRE1α and XBP-1s. β-actin was probed as loading control. The intensity is expressed as mean ± SEM of the percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis or unpaired Student’s t-test. ( B ) TUNEL, p-JNK, and Caspae-12 were effectively eliminated as measured by immunohistochemistry method. Data are expressed as mean ± SEM of the percentage of the respective controls and analyzed using one-way ANOVA with Tamhane’s T2 analysis or unpaired Student’s t-test. ( C ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against GRP78, CHOP, Bcl-2, Bax and Cleaved-Caspase-3. β-actin was probed as loading control. Results of are expressed as mean ± SEM indicated as percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis (GRP78, Cleaved-Caspase-3, CHOP) or Tamhane’s T2 analysis (Bcl-2, Bax) or unpaired Student’s t-test. Δ P

    Article Snippet: The primary antibodies were as follows: mouse anti-IRE1α (sc-390960; 1: 1000; Santa Cruz), rabbit anti-P-IRE1α (ab48187; 1: 2000; abcam), mouse anti-XBP1 (sc-8015; 1: 1000; Abcam), mouse anti-GRP78 (sc-376768; 1: 1000; Santa Cruz), mouse anti-GADD153 (sc-7351; 1: 500; Santa Cruz), rabbit anti-Caspase-3 (ab13847; 1: 1000; abcam), rabbit anti-Caspase-12 (om273459; 1: 1000; Omnimabs), mouse anti-Bcl-2 (sc-7382; 1: 1000; Santa Cruz), mouse anti-Bax (sc-7480; 1: 1000; Santa Cruz), mouse anti-p-JNK (sc-6254; 1: 1000; Santa Cruz), and rabbit anti-PGP9.5 (ab108986; 1: 2000; Abcam).

    Techniques: Inhibition, Injection, Western Blot, TUNEL Assay, Immunohistochemistry

    Expression of CHOP, Bcl-2, Bax, p-JNK, Caspase-12 and cleaved-Caspase-3 in RSC96 cells after IRE1α siRNA transfection and exposure to high glucose. ( A ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against CHOP, Bcl-2, Bax, p-JNK, Caspase-12 and cleaved-Caspase-3. ( B ) The band intensity expressed as mean ± SEM of the percentage of the respective 25 mM glucose-treated cells and analyzed using one-way ANOVA with LSD analysis (CHOP, Bcl-2/48 h, Bax/48 h, p-JNK/24 h, Caspase-12, cleaved-Caspase-3,) or Tamhane’s T2 analysis (p-JNK/48 h, Bcl-2/24 h, Bax/24 h, Caspase-3/24 h,). Δ P

    Journal: Scientific Reports

    Article Title: IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro

    doi: 10.1038/s41598-018-20950-9

    Figure Lengend Snippet: Expression of CHOP, Bcl-2, Bax, p-JNK, Caspase-12 and cleaved-Caspase-3 in RSC96 cells after IRE1α siRNA transfection and exposure to high glucose. ( A ) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against CHOP, Bcl-2, Bax, p-JNK, Caspase-12 and cleaved-Caspase-3. ( B ) The band intensity expressed as mean ± SEM of the percentage of the respective 25 mM glucose-treated cells and analyzed using one-way ANOVA with LSD analysis (CHOP, Bcl-2/48 h, Bax/48 h, p-JNK/24 h, Caspase-12, cleaved-Caspase-3,) or Tamhane’s T2 analysis (p-JNK/48 h, Bcl-2/24 h, Bax/24 h, Caspase-3/24 h,). Δ P

    Article Snippet: The primary antibodies were as follows: mouse anti-IRE1α (sc-390960; 1: 1000; Santa Cruz), rabbit anti-P-IRE1α (ab48187; 1: 2000; abcam), mouse anti-XBP1 (sc-8015; 1: 1000; Abcam), mouse anti-GRP78 (sc-376768; 1: 1000; Santa Cruz), mouse anti-GADD153 (sc-7351; 1: 500; Santa Cruz), rabbit anti-Caspase-3 (ab13847; 1: 1000; abcam), rabbit anti-Caspase-12 (om273459; 1: 1000; Omnimabs), mouse anti-Bcl-2 (sc-7382; 1: 1000; Santa Cruz), mouse anti-Bax (sc-7480; 1: 1000; Santa Cruz), mouse anti-p-JNK (sc-6254; 1: 1000; Santa Cruz), and rabbit anti-PGP9.5 (ab108986; 1: 2000; Abcam).

    Techniques: Expressing, Transfection, Western Blot