anti cii igg antibody  (Chondrex Inc)


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    Chondrex Inc anti cii igg antibody
    Anti Cii Igg Antibody, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    serum igg anti cii antibody  (Chondrex Inc)


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    Chondrex Inc serum igg anti cii antibody
    Serum Igg Anti Cii Antibody, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse type ii collagen specific igg anti cii antibody  (Chondrex Inc)


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    Chondrex Inc mouse type ii collagen specific igg anti cii antibody
    Mouse Type Ii Collagen Specific Igg Anti Cii Antibody, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse type ii collagen specific igg anti cii antibody  (Chondrex Inc)


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    Chondrex Inc mouse type ii collagen specific igg anti cii antibody
    Mouse Type Ii Collagen Specific Igg Anti Cii Antibody, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cii immunoglobulin g igg levels  (Chondrex Inc)


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    Chondrex Inc cii immunoglobulin g igg levels
    Cii Immunoglobulin G Igg Levels, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti bovine cii igg antibody titer  (Chondrex Inc)


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    anti cii igg antibody titers  (Chondrex Inc)


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    Chondrex Inc anti cii igg antibody titers
    Effect of KPs on <t>CII-specific</t> immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen <t>(IgG)</t> antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.
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    1) Product Images from "Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice"

    Article Title: Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.721594

    Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.
    Figure Legend Snippet: Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Radioactivity, Incubation, Flow Cytometry

    anti mouse cii igg tmb  (Chondrex Inc)


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    Chondrex Inc anti mouse cii igg tmb
    Effect of KPs on <t>CII-specific</t> immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen <t>(IgG)</t> antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.
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    1) Product Images from "Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice"

    Article Title: Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.721594

    Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.
    Figure Legend Snippet: Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Radioactivity, Incubation, Flow Cytometry

    bovine cii igg1  (Chondrex Inc)


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    Chondrex Inc bovine cii igg1
    WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) <t>CII</t> reactive <t>IgG1</t> and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001
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    1) Product Images from "Activation of formyl peptide receptor 1 elicits therapeutic effects against collagen‐induced arthritis"

    Article Title: Activation of formyl peptide receptor 1 elicits therapeutic effects against collagen‐induced arthritis

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.16854

    WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) CII reactive IgG1 and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001
    Figure Legend Snippet: WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) CII reactive IgG1 and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001

    Techniques Used: Injection, Staining

    anti chicken collagen type ii cii igg  (Chondrex Inc)


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    Chondrex Inc anti chicken collagen type ii cii igg
    IL-17RD shedding occurs in macrophages under inflammatory conditions. a Induction of IL17RD expression in the human monocytic U937 cell line treated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) was determined by RT-PCR. β-actin was used as a control. b Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of U937 cells treated with PMA for the indicated times. c Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of RAW264.7 cells treated with TNF-α (20 ng/mL) for the indicated times. d Induction of Il17rd expression in bone marrow cells (BMCs) or bone marrow-derived macrophages (BMDMs) was determined by RT-PCR. e ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with TNF-α. f sIL-17RD in the supernatants of BMDMs stimulated with or without TNF-α was immunoprecipitated with the 3F12 antibody or control <t>IgG</t> and analyzed by western blotting with the 3F12 antibody. D dimer, M monomer, IP immunoprecipitation. g ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with other cytokines. h sIL-17RD in the serum of mice treated without (control) or with collagen type II <t>(CII)-induced</t> arthritis (CIA) was measured by ELISA. Each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the unstimulated control (two-way ANOVA)
    Anti Chicken Collagen Type Ii Cii Igg, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering"

    Article Title: A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering

    Journal: Cellular and Molecular Immunology

    doi: 10.1038/s41423-020-00548-w

    IL-17RD shedding occurs in macrophages under inflammatory conditions. a Induction of IL17RD expression in the human monocytic U937 cell line treated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) was determined by RT-PCR. β-actin was used as a control. b Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of U937 cells treated with PMA for the indicated times. c Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of RAW264.7 cells treated with TNF-α (20 ng/mL) for the indicated times. d Induction of Il17rd expression in bone marrow cells (BMCs) or bone marrow-derived macrophages (BMDMs) was determined by RT-PCR. e ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with TNF-α. f sIL-17RD in the supernatants of BMDMs stimulated with or without TNF-α was immunoprecipitated with the 3F12 antibody or control IgG and analyzed by western blotting with the 3F12 antibody. D dimer, M monomer, IP immunoprecipitation. g ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with other cytokines. h sIL-17RD in the serum of mice treated without (control) or with collagen type II (CII)-induced arthritis (CIA) was measured by ELISA. Each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the unstimulated control (two-way ANOVA)
    Figure Legend Snippet: IL-17RD shedding occurs in macrophages under inflammatory conditions. a Induction of IL17RD expression in the human monocytic U937 cell line treated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) was determined by RT-PCR. β-actin was used as a control. b Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of U937 cells treated with PMA for the indicated times. c Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of RAW264.7 cells treated with TNF-α (20 ng/mL) for the indicated times. d Induction of Il17rd expression in bone marrow cells (BMCs) or bone marrow-derived macrophages (BMDMs) was determined by RT-PCR. e ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with TNF-α. f sIL-17RD in the supernatants of BMDMs stimulated with or without TNF-α was immunoprecipitated with the 3F12 antibody or control IgG and analyzed by western blotting with the 3F12 antibody. D dimer, M monomer, IP immunoprecipitation. g ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with other cytokines. h sIL-17RD in the serum of mice treated without (control) or with collagen type II (CII)-induced arthritis (CIA) was measured by ELISA. Each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the unstimulated control (two-way ANOVA)

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Derivative Assay, Enzyme-linked Immunosorbent Assay, Immunoprecipitation

    sIL-17RD accelerates CII-induced arthritis development and the inflammatory response. a Schematic showing the injection of purified sIL-17RD or GFP protein into DBA/1J mice with collagen type II (CII)-induced arthritis (CIA). b The paws of mice in the indicated groups were immunized with collagen II for 6 weeks. Clinical arthritis score (c) and mid hind paw thickness (d) of DBA/1J mice with CIA that were injected with GFP or sIL-17RD proteins. The data are presented as the mean clinical score. *p < 0.05, **p < 0.01 versus the control GFP-injected mice. Hematoxylin and eosin (H&E)-stained sections (e) and H&E score (F) of ankle joints in the mice of the indicated groups. **p < 0.01 versus the control GFP-injected mice. g The incidence of arthritis in each group. h Serum levels of mouse anti-type II collagen IgG, as measured by ELISA. i–l Serum levels of IL-17, IL-6, TNF-α, and IL-10, as measured by ELISA. Six mice were used in each group, and each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001 versus the control GFP-injected mice
    Figure Legend Snippet: sIL-17RD accelerates CII-induced arthritis development and the inflammatory response. a Schematic showing the injection of purified sIL-17RD or GFP protein into DBA/1J mice with collagen type II (CII)-induced arthritis (CIA). b The paws of mice in the indicated groups were immunized with collagen II for 6 weeks. Clinical arthritis score (c) and mid hind paw thickness (d) of DBA/1J mice with CIA that were injected with GFP or sIL-17RD proteins. The data are presented as the mean clinical score. *p < 0.05, **p < 0.01 versus the control GFP-injected mice. Hematoxylin and eosin (H&E)-stained sections (e) and H&E score (F) of ankle joints in the mice of the indicated groups. **p < 0.01 versus the control GFP-injected mice. g The incidence of arthritis in each group. h Serum levels of mouse anti-type II collagen IgG, as measured by ELISA. i–l Serum levels of IL-17, IL-6, TNF-α, and IL-10, as measured by ELISA. Six mice were used in each group, and each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001 versus the control GFP-injected mice

    Techniques Used: Injection, Purification, Staining, Enzyme-linked Immunosorbent Assay

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    Chondrex Inc anti cii igg antibody
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    Effect of KPs on <t>CII-specific</t> immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen <t>(IgG)</t> antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.
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    Effect of KPs on <t>CII-specific</t> immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen <t>(IgG)</t> antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.
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    WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) <t>CII</t> reactive <t>IgG1</t> and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001
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    IL-17RD shedding occurs in macrophages under inflammatory conditions. a Induction of IL17RD expression in the human monocytic U937 cell line treated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) was determined by RT-PCR. β-actin was used as a control. b Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of U937 cells treated with PMA for the indicated times. c Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of RAW264.7 cells treated with TNF-α (20 ng/mL) for the indicated times. d Induction of Il17rd expression in bone marrow cells (BMCs) or bone marrow-derived macrophages (BMDMs) was determined by RT-PCR. e ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with TNF-α. f sIL-17RD in the supernatants of BMDMs stimulated with or without TNF-α was immunoprecipitated with the 3F12 antibody or control <t>IgG</t> and analyzed by western blotting with the 3F12 antibody. D dimer, M monomer, IP immunoprecipitation. g ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with other cytokines. h sIL-17RD in the serum of mice treated without (control) or with collagen type II <t>(CII)-induced</t> arthritis (CIA) was measured by ELISA. Each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the unstimulated control (two-way ANOVA)
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    Image Search Results


    Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.

    Journal: Frontiers in Pharmacology

    Article Title: Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice

    doi: 10.3389/fphar.2021.721594

    Figure Lengend Snippet: Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.

    Article Snippet: The anti-CII IgG antibody titers were analyzed with commercially available kits and anti-mouse CII IgG TMB (2036T; Chondrex, Redmond, WA, United States) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Radioactivity, Incubation, Flow Cytometry

    Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.

    Journal: Frontiers in Pharmacology

    Article Title: Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice

    doi: 10.3389/fphar.2021.721594

    Figure Lengend Snippet: Effect of KPs on CII-specific immune responses in serum and the CD4 + T cell subset profiles in the spleens of CIA mice. (A) Sera were collected by orbital sinus venipuncture at day 42 after primary immunization to estimate anti-type II collagen (IgG) antibody levels by ELISA. (B) Splenocytes were harvested at day 42. Cultures were pulsed with 3 H-thymidine (1 μCi/well) and the incorporation of radioactivity was quantified in counts per minute (cpm) by liquid scintillation counting after 18 h of incubation. Intracellular flow cytometry analysis of cells collected from the spleens of the mice in different groups on day 42 after immunization. Flow cytometry analysis of Th1 and Th17 cells. The cells were gated on the CD4 + population. Bar graph of the quantification of CD4 + T cell subsets, Th1 (C) and Th17 (D) , plotted from three independent experiments. The data are expressed as the mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001 vs the CIA/vehicle control group, as determined by one-way ANOVA with Dunnett’s test.

    Article Snippet: The anti-CII IgG antibody titers were analyzed with commercially available kits and anti-mouse CII IgG TMB (2036T; Chondrex, Redmond, WA, United States) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Radioactivity, Incubation, Flow Cytometry

    WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) CII reactive IgG1 and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Activation of formyl peptide receptor 1 elicits therapeutic effects against collagen‐induced arthritis

    doi: 10.1111/jcmm.16854

    Figure Lengend Snippet: WKYMVm administration elicits beneficial effects against CIA. (A‐D) Vehicle or WKYMVm (4 mg/kg) was subcutaneously injected daily into CIA mice from secondary boosting at day 21. Mice were sacrificed on day 35. (A) Paw thickness (top) and clinical scores (bottom) of CIA mice were monitored during the indicated period. (B) Paw swelling of the two groups compared at the day of sacrifice. Representative images are shown. (C) CII reactive IgG1 and IgG2a were measured in peripheral blood serum from each group of mice. (D) Joint tissue sections were stained with H&E and safranin O (×40). Representative figures are shown. Data are expressed as the mean ± SEM ( n = 4–6 per group). p values were calculated by two‐way ANOVA (A) or Student's t test (C). * p < 0.05, *** p < 0.001

    Article Snippet: The levels of IgG1 and IgG2a reactive to immunized collagen in the peripheral blood serum were determined by using a mouse anti‐bovine CII IgG1 and IgG2a antibody assay kit with tetramethylbenzidine (TMB) substrate (Chondrex, Redmond, WA, USA).

    Techniques: Injection, Staining

    IL-17RD shedding occurs in macrophages under inflammatory conditions. a Induction of IL17RD expression in the human monocytic U937 cell line treated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) was determined by RT-PCR. β-actin was used as a control. b Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of U937 cells treated with PMA for the indicated times. c Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of RAW264.7 cells treated with TNF-α (20 ng/mL) for the indicated times. d Induction of Il17rd expression in bone marrow cells (BMCs) or bone marrow-derived macrophages (BMDMs) was determined by RT-PCR. e ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with TNF-α. f sIL-17RD in the supernatants of BMDMs stimulated with or without TNF-α was immunoprecipitated with the 3F12 antibody or control IgG and analyzed by western blotting with the 3F12 antibody. D dimer, M monomer, IP immunoprecipitation. g ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with other cytokines. h sIL-17RD in the serum of mice treated without (control) or with collagen type II (CII)-induced arthritis (CIA) was measured by ELISA. Each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the unstimulated control (two-way ANOVA)

    Journal: Cellular and Molecular Immunology

    Article Title: A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering

    doi: 10.1038/s41423-020-00548-w

    Figure Lengend Snippet: IL-17RD shedding occurs in macrophages under inflammatory conditions. a Induction of IL17RD expression in the human monocytic U937 cell line treated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) was determined by RT-PCR. β-actin was used as a control. b Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of U937 cells treated with PMA for the indicated times. c Immunoblot analysis of sIL-17RD (S) in the supernatants and IL-17RD (FL) in the cell lysates of RAW264.7 cells treated with TNF-α (20 ng/mL) for the indicated times. d Induction of Il17rd expression in bone marrow cells (BMCs) or bone marrow-derived macrophages (BMDMs) was determined by RT-PCR. e ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with TNF-α. f sIL-17RD in the supernatants of BMDMs stimulated with or without TNF-α was immunoprecipitated with the 3F12 antibody or control IgG and analyzed by western blotting with the 3F12 antibody. D dimer, M monomer, IP immunoprecipitation. g ELISA analysis of sIL-17RD in the supernatants of BMDMs stimulated with other cytokines. h sIL-17RD in the serum of mice treated without (control) or with collagen type II (CII)-induced arthritis (CIA) was measured by ELISA. Each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared to the unstimulated control (two-way ANOVA)

    Article Snippet: The level of anti-chicken collagen type II (CII) IgG in serum was analyzed using ELISA (Chondrex, USA) according to the manufacturer’s protocol.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Derivative Assay, Enzyme-linked Immunosorbent Assay, Immunoprecipitation

    sIL-17RD accelerates CII-induced arthritis development and the inflammatory response. a Schematic showing the injection of purified sIL-17RD or GFP protein into DBA/1J mice with collagen type II (CII)-induced arthritis (CIA). b The paws of mice in the indicated groups were immunized with collagen II for 6 weeks. Clinical arthritis score (c) and mid hind paw thickness (d) of DBA/1J mice with CIA that were injected with GFP or sIL-17RD proteins. The data are presented as the mean clinical score. *p < 0.05, **p < 0.01 versus the control GFP-injected mice. Hematoxylin and eosin (H&E)-stained sections (e) and H&E score (F) of ankle joints in the mice of the indicated groups. **p < 0.01 versus the control GFP-injected mice. g The incidence of arthritis in each group. h Serum levels of mouse anti-type II collagen IgG, as measured by ELISA. i–l Serum levels of IL-17, IL-6, TNF-α, and IL-10, as measured by ELISA. Six mice were used in each group, and each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001 versus the control GFP-injected mice

    Journal: Cellular and Molecular Immunology

    Article Title: A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering

    doi: 10.1038/s41423-020-00548-w

    Figure Lengend Snippet: sIL-17RD accelerates CII-induced arthritis development and the inflammatory response. a Schematic showing the injection of purified sIL-17RD or GFP protein into DBA/1J mice with collagen type II (CII)-induced arthritis (CIA). b The paws of mice in the indicated groups were immunized with collagen II for 6 weeks. Clinical arthritis score (c) and mid hind paw thickness (d) of DBA/1J mice with CIA that were injected with GFP or sIL-17RD proteins. The data are presented as the mean clinical score. *p < 0.05, **p < 0.01 versus the control GFP-injected mice. Hematoxylin and eosin (H&E)-stained sections (e) and H&E score (F) of ankle joints in the mice of the indicated groups. **p < 0.01 versus the control GFP-injected mice. g The incidence of arthritis in each group. h Serum levels of mouse anti-type II collagen IgG, as measured by ELISA. i–l Serum levels of IL-17, IL-6, TNF-α, and IL-10, as measured by ELISA. Six mice were used in each group, and each experiment was repeated three times. The values are the mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001 versus the control GFP-injected mice

    Article Snippet: The level of anti-chicken collagen type II (CII) IgG in serum was analyzed using ELISA (Chondrex, USA) according to the manufacturer’s protocol.

    Techniques: Injection, Purification, Staining, Enzyme-linked Immunosorbent Assay