anti cholinergic muscarinic receptor 1  (Alomone Labs)


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    Alomone Labs anti cholinergic muscarinic receptor 1
    Anti Cholinergic Muscarinic Receptor 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti cholinergic muscarinic receptor 1  (Alomone Labs)


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    Alomone Labs anti cholinergic muscarinic receptor 1
    Anti Cholinergic Muscarinic Receptor 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m1 muscarinic receptor  (Alomone Labs)


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    Alomone Labs m1 muscarinic receptor
    M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m1r antibodies  (Alomone Labs)


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    Alomone Labs m1r antibodies
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
    M1r Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice"

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    Journal: IBRO Neuroscience Reports

    doi: 10.1016/j.ibneur.2021.09.005

    M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
    Figure Legend Snippet: M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.

    Techniques Used: Labeling, Blocking Assay

    Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.
    Figure Legend Snippet: Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.

    Techniques Used: Labeling

    Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.
    Figure Legend Snippet: Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.

    Techniques Used: Labeling

     M1R-labeled  axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.
    Figure Legend Snippet: M1R-labeled axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.

    Techniques Used: Expressing

    Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.
    Figure Legend Snippet: Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.

    Techniques Used: Labeling, Blocking Assay

    CB1R-terminals forming symmetric or asymmetric synapses with  M1R-labeled  or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.
    Figure Legend Snippet: CB1R-terminals forming symmetric or asymmetric synapses with M1R-labeled or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.

    Techniques Used:

    anti chrm1 antibody  (Alomone Labs)


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    Alomone Labs anti chrm1 antibody
    Anti Chrm1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti muscarinic receptor  (Alomone Labs)


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    Alomone Labs rabbit anti muscarinic receptor
    Rabbit Anti Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human m1 polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit anti human m1 polyclonal antibody
    Rabbit Anti Human M1 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mab alomone  (Alomone Labs)


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    Alomone Labs mab alomone
    Mab Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti m1 muscarinic receptor  (Alomone Labs)


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    Alomone Labs rabbit anti m1 muscarinic receptor
    <t>(A)</t> <t>Calretinin</t> (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) <t>M1</t> muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
    Rabbit Anti M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Muscarinic modulation of M and h currents in gerbil spherical bushy cells"

    Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0226954

    (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
    Figure Legend Snippet: (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.

    Techniques Used: Staining

    On one hand, acetylcholine (Ach) binds the M1/M3 muscarinic receptor which induces the activation of the phospholipase C (PLC) via the Gα q/11 protein. The activated PLC depletes the phosphatidylinositol biphosphate (PIP 2 ) into diacylglycerol (DAG) and inositol triphosphate (IP 3 ). IP 3 concentration increase releases calcium from the internal cell storage. Directly or indirectly, one or several of these 2 nd messengers trigger the closure of Kv7 channels thereby depolarizing the cell membrane. On the other hand, acetylcholine may bind the M2 muscarinic receptor inhibiting the adenylate cyclase (AC) via the Gα i protein which induces a reduction of cyclic AMP. Thus, for the same membrane potential, more HCN channels are closed inducing the hyperpolarization of the cell. Cell membrane and ion channels were designed by “ Servier Medical Art ” ( https://smart.servier.com ) provided by Les Laboratoires Servier ( https://servier.com/en ), licensed under Creative Commons Attribution 3.0 Unported License .
    Figure Legend Snippet: On one hand, acetylcholine (Ach) binds the M1/M3 muscarinic receptor which induces the activation of the phospholipase C (PLC) via the Gα q/11 protein. The activated PLC depletes the phosphatidylinositol biphosphate (PIP 2 ) into diacylglycerol (DAG) and inositol triphosphate (IP 3 ). IP 3 concentration increase releases calcium from the internal cell storage. Directly or indirectly, one or several of these 2 nd messengers trigger the closure of Kv7 channels thereby depolarizing the cell membrane. On the other hand, acetylcholine may bind the M2 muscarinic receptor inhibiting the adenylate cyclase (AC) via the Gα i protein which induces a reduction of cyclic AMP. Thus, for the same membrane potential, more HCN channels are closed inducing the hyperpolarization of the cell. Cell membrane and ion channels were designed by “ Servier Medical Art ” ( https://smart.servier.com ) provided by Les Laboratoires Servier ( https://servier.com/en ), licensed under Creative Commons Attribution 3.0 Unported License .

    Techniques Used: Activation Assay, Concentration Assay

    amr 010 ab 2340994 rabbit anti m2 muscarinic receptor alomone  (Alomone Labs)


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    Alomone Labs amr 010 ab 2340994 rabbit anti m2 muscarinic receptor alomone
    Amr 010 Ab 2340994 Rabbit Anti M2 Muscarinic Receptor Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti m1 muscarinic receptor  (Alomone Labs)


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    Alomone Labs rabbit anti m1 muscarinic receptor
    Primary antibodies used
    Rabbit Anti M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti m1 muscarinic receptor/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "Cholinergic activation of enteric glia is a physiological mechanism that contributes to the regulation of gastrointestinal motility"

    Article Title: Cholinergic activation of enteric glia is a physiological mechanism that contributes to the regulation of gastrointestinal motility

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00155.2018

    Primary antibodies used
    Figure Legend Snippet: Primary antibodies used

    Techniques Used:

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    Alomone Labs m1r antibodies
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    Alomone Labs mab alomone
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    Alomone Labs rabbit anti m1 muscarinic receptor
    <t>(A)</t> <t>Calretinin</t> (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) <t>M1</t> muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
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    Alomone Labs amr 010 ab 2340994 rabbit anti m2 muscarinic receptor alomone
    <t>(A)</t> <t>Calretinin</t> (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) <t>M1</t> muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.
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    Image Search Results


    M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling, Blocking Assay

    Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling

    Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling

     M1R-labeled  axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: M1R-labeled axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Expressing

    Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling, Blocking Assay

    CB1R-terminals forming symmetric or asymmetric synapses with  M1R-labeled  or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: CB1R-terminals forming symmetric or asymmetric synapses with M1R-labeled or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques:

    (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.

    Journal: PLoS ONE

    Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

    doi: 10.1371/journal.pone.0226954

    Figure Lengend Snippet: (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.

    Article Snippet: The primary antibodies used were: goat anti-calretinin antibody (1/500, Merck Millipore, Germany), rabbit anti-M1 muscarinic receptor (443–458) antibody (1/200, #AMR-010, RRID: AB_2340994, Alomone labs, Israel) and rabbit anti-M2 muscarinic receptor antibody (1/200, #AMR-002, RRID: AB_2039995, Alomone labs, Israel).

    Techniques: Staining

    On one hand, acetylcholine (Ach) binds the M1/M3 muscarinic receptor which induces the activation of the phospholipase C (PLC) via the Gα q/11 protein. The activated PLC depletes the phosphatidylinositol biphosphate (PIP 2 ) into diacylglycerol (DAG) and inositol triphosphate (IP 3 ). IP 3 concentration increase releases calcium from the internal cell storage. Directly or indirectly, one or several of these 2 nd messengers trigger the closure of Kv7 channels thereby depolarizing the cell membrane. On the other hand, acetylcholine may bind the M2 muscarinic receptor inhibiting the adenylate cyclase (AC) via the Gα i protein which induces a reduction of cyclic AMP. Thus, for the same membrane potential, more HCN channels are closed inducing the hyperpolarization of the cell. Cell membrane and ion channels were designed by “ Servier Medical Art ” ( https://smart.servier.com ) provided by Les Laboratoires Servier ( https://servier.com/en ), licensed under Creative Commons Attribution 3.0 Unported License .

    Journal: PLoS ONE

    Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

    doi: 10.1371/journal.pone.0226954

    Figure Lengend Snippet: On one hand, acetylcholine (Ach) binds the M1/M3 muscarinic receptor which induces the activation of the phospholipase C (PLC) via the Gα q/11 protein. The activated PLC depletes the phosphatidylinositol biphosphate (PIP 2 ) into diacylglycerol (DAG) and inositol triphosphate (IP 3 ). IP 3 concentration increase releases calcium from the internal cell storage. Directly or indirectly, one or several of these 2 nd messengers trigger the closure of Kv7 channels thereby depolarizing the cell membrane. On the other hand, acetylcholine may bind the M2 muscarinic receptor inhibiting the adenylate cyclase (AC) via the Gα i protein which induces a reduction of cyclic AMP. Thus, for the same membrane potential, more HCN channels are closed inducing the hyperpolarization of the cell. Cell membrane and ion channels were designed by “ Servier Medical Art ” ( https://smart.servier.com ) provided by Les Laboratoires Servier ( https://servier.com/en ), licensed under Creative Commons Attribution 3.0 Unported License .

    Article Snippet: The primary antibodies used were: goat anti-calretinin antibody (1/500, Merck Millipore, Germany), rabbit anti-M1 muscarinic receptor (443–458) antibody (1/200, #AMR-010, RRID: AB_2340994, Alomone labs, Israel) and rabbit anti-M2 muscarinic receptor antibody (1/200, #AMR-002, RRID: AB_2039995, Alomone labs, Israel).

    Techniques: Activation Assay, Concentration Assay