anti chikv igm  (Abcam)

 
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    Name:
    Human Anti Chikungunya Virus IgM ELISA Kit
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    Catalog Number:
    ab177848
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    Abcam anti chikv igm
    Comparison of <t>EILV/CHIKV</t> and Abcam human anti-CHIKV <t>IgM-capture</t> ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    https://www.bioz.com/result/anti chikv igm/product/Abcam
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti chikv igm - by Bioz Stars, 2020-08
    93/100 stars

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    1) Product Images from "Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection"

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0004119

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.
    Figure Legend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.
    Figure Legend Snippet: Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Techniques Used:

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection
    Article Snippet: .. Commercially available anti-CHIKV IgM (ab177848, Lot: GR195090-3, Abcam, Cambridge, MA) and anti-CHIKV IgG Human ELISA Kits (ab177835, Lot: GR148047-1, Abcam) were used according to manufacturer’s instructions. ..

    Article Title: Development of an E2 ELISA Methodology to Assess Chikungunya Seroprevalence in Patients from an Endemic Region of Mexico
    Article Snippet: .. Detection of Anti-CHIKV IgM and IgG by Commercial ELISA Kit For the qualitative measurement of IgM and IgG class antibodies against CHIKV in human serum, an Anti-Chikungunya Virus IgM Human ELISA Kit (ab 177848) and an IgG Human ELISA Kit (ab 177835) from Abcam were used to test all the serum samples according to the manufacturer’s protocols. .. Maps and Geographical Data Maps in A–C were obtained using the QGIS 3.6 software Release 3.6 and the QGIS Geographic Information System, using the ESRI satellite database.

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    Abcam anti chikv igm elisa
    Comparison of sensitivity by serum titration. Chikungunya virus <t>(CHIKV)</t> <t>IgM+</t> samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.
    Anti Chikv Igm Elisa, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chikv igm elisa/product/Abcam
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chikv igm elisa - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    93
    Abcam anti chikv igm human elisa kit
    Comparison of sensitivity by serum titration. Chikungunya virus <t>(CHIKV)</t> <t>IgM+</t> samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.
    Anti Chikv Igm Human Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chikv igm human elisa kit/product/Abcam
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti chikv igm human elisa kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Article Snippet: The Abcam Anti-CHIKV IgM ELISA had high performance at CARPHA, but at CDC, performance was inconsistent between lots.

    Techniques: Titration

    Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Article Snippet: The Abcam Anti-CHIKV IgM ELISA had high performance at CARPHA, but at CDC, performance was inconsistent between lots.

    Techniques: Positive Control

    Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Article Snippet: The Abcam Anti-CHIKV IgM ELISA had high performance at CARPHA, but at CDC, performance was inconsistent between lots.

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control

    Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Article Snippet: The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA.

    Techniques: Titration

    Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Article Snippet: The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA.

    Techniques: Positive Control

    Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Article Snippet: The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA.

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control

    Age-related impairment of adaptive immune response to CHIKV. (A) Popliteal LNs collected and quantified from either naïve or CHIKV-infected A and O mice at day 3, 7, or 9 post-infection. The LN draining from the CHIKV-inoculated foot is indicated as dLN and from the non-inoculated foot as ndLN. Table under graph indicates the average fold-increase from naïve for each age in either the dLN or ndLN ( n = 6–8 per group). Horizontal lines indicate the median. Statistical significance determined by student’s t -test. (B-C) Lymphocytes from popliteal LNs on d7 post-infection were stimulated with CHIKV peptides in the presence of protein transport inhibitor. Total number of IFNγ + CD4 T cells (B) and frequency (C) for each age. Data are mean ± SEM ( n = 10 per group). Statistical significance determined by unpaired Student’s t -test. (D) CHIKV-specific IgM and (E) IgG2c in serum determined by ELISA at the indicated day post-infection. Data are mean ( n = 4–24 per group). Statistical significance was determined by two-way ANOVA with Bonferroni post-test. (F) Plaque reduction neutralizing test on serum from days 9 and 60 post-infection. Data are mean + SEM ( n = 12 per group). Statistical significance was determined by two-way ANOVA with Bonferroni post-test. (G) Serum samples collected from patients experiencing acute CHIKV-disease were evaluated by plaque reduction neutralizing test. Data are mean + SEM ( n = 24 young and 15 aged). Statistical significance was evaluated by unpaired student’s t -test. In all panels black indicates A, red indicates O; * P

    Journal: PLoS Pathogens

    Article Title: Dysregulated TGF-β Production Underlies the Age-Related Vulnerability to Chikungunya Virus

    doi: 10.1371/journal.ppat.1005891

    Figure Lengend Snippet: Age-related impairment of adaptive immune response to CHIKV. (A) Popliteal LNs collected and quantified from either naïve or CHIKV-infected A and O mice at day 3, 7, or 9 post-infection. The LN draining from the CHIKV-inoculated foot is indicated as dLN and from the non-inoculated foot as ndLN. Table under graph indicates the average fold-increase from naïve for each age in either the dLN or ndLN ( n = 6–8 per group). Horizontal lines indicate the median. Statistical significance determined by student’s t -test. (B-C) Lymphocytes from popliteal LNs on d7 post-infection were stimulated with CHIKV peptides in the presence of protein transport inhibitor. Total number of IFNγ + CD4 T cells (B) and frequency (C) for each age. Data are mean ± SEM ( n = 10 per group). Statistical significance determined by unpaired Student’s t -test. (D) CHIKV-specific IgM and (E) IgG2c in serum determined by ELISA at the indicated day post-infection. Data are mean ( n = 4–24 per group). Statistical significance was determined by two-way ANOVA with Bonferroni post-test. (F) Plaque reduction neutralizing test on serum from days 9 and 60 post-infection. Data are mean + SEM ( n = 12 per group). Statistical significance was determined by two-way ANOVA with Bonferroni post-test. (G) Serum samples collected from patients experiencing acute CHIKV-disease were evaluated by plaque reduction neutralizing test. Data are mean + SEM ( n = 24 young and 15 aged). Statistical significance was evaluated by unpaired student’s t -test. In all panels black indicates A, red indicates O; * P

    Article Snippet: Samples were tested using the Anti-CHIKV IgM human ELISA kit (Abcam, Cambridge, MA, USA).

    Techniques: Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Dysregulated cytokine production with age. Serum was collected from A and O mice and assayed by ELISA for (A) CXCL9 or (B) TGFβ concentration at days 2 or 9 and 30, respectively. Data are mean + SEM ( n = 3 naïve and 7–8 infected per age). (C) Human samples from IgM-positive CHIKV patients or age and sex-matched controls were assayed for Free-active TGFβ cytokine by ELISA. Data are mean + SEM ( n = 39 each group). Statistical significance was evaluated by unpaired student’s t -test. * P

    Journal: PLoS Pathogens

    Article Title: Dysregulated TGF-β Production Underlies the Age-Related Vulnerability to Chikungunya Virus

    doi: 10.1371/journal.ppat.1005891

    Figure Lengend Snippet: Dysregulated cytokine production with age. Serum was collected from A and O mice and assayed by ELISA for (A) CXCL9 or (B) TGFβ concentration at days 2 or 9 and 30, respectively. Data are mean + SEM ( n = 3 naïve and 7–8 infected per age). (C) Human samples from IgM-positive CHIKV patients or age and sex-matched controls were assayed for Free-active TGFβ cytokine by ELISA. Data are mean + SEM ( n = 39 each group). Statistical significance was evaluated by unpaired student’s t -test. * P

    Article Snippet: Samples were tested using the Anti-CHIKV IgM human ELISA kit (Abcam, Cambridge, MA, USA).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Infection

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: