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Abcam anti chikv igm
Anti Chikv Igm, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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anti chikv igm - by Bioz Stars, 2021-05
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Enzyme-linked Immunosorbent Assay:

Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection
Article Snippet: .. Commercially available anti-CHIKV IgM (ab177848, Lot: GR195090-3, Abcam, Cambridge, MA) and anti-CHIKV IgG Human ELISA Kits (ab177835, Lot: GR148047-1, Abcam) were used according to manufacturer’s instructions. ..

Article Title: Demographic and clinical characteristics of chikungunya patients from six Colombian cities, 2014–2015
Article Snippet: Because our population was immunologically naïve (there were no reports of CHIKV infection prior to this epidemic) we considered as positive the presence of virus-specific IgG antibodies in single serum sample during any stage of the disease. .. CHIKV serology Enzyme-linked immunosorbent assay (ELISA) was performed according to the manufacturer’s instructions (Abcam® ab177848 anti-CHIKV IgM human ELISA kit and ab177835 anti-CHIKV IgG human ELISA kit, Abcam, Cambridge, UK). .. Abcam’s anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the Centers for Disease Control and Prevention (CDC) IgM ELISA [ , ].

Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays
Article Snippet: .. In summary, the Euroimmun Anti-CHIKV ELISA (IgM), InBios CHIKjj Detect MAC-ELISA, Abcam Anti-CHIKV IgM human ELISA (with CHIKV-specific IgM serum controls), and Euroimmun Anti-CHIKV IIFT kits were shown to have equivalent performance to the reference assays by which they were evaluated. ..

Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays
Article Snippet: .. The Abcam Anti-CHIKV IgM ELISA had high performance at CARPHA, but at CDC, performance was inconsistent between lots. .. After replacement of the biotinylated IgM antibody controls with serum containing CHIKV-specific IgM and additional quality assurance/control measures, the Abcam kit was rereleased and reevaluated at CDC.

Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays
Article Snippet: .. The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA. ..

Article Title: Demographic and clinical characteristics of chikungunya patients from six Colombian cities, 2014–2015
Article Snippet: CHIKV serology Enzyme-linked immunosorbent assay (ELISA) was performed according to the manufacturer’s instructions (Abcam® ab177848 anti-CHIKV IgM human ELISA kit and ab177835 anti-CHIKV IgG human ELISA kit, Abcam, Cambridge, UK). .. Abcam’s anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the Centers for Disease Control and Prevention (CDC) IgM ELISA [ , ]. ..

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    Abcam anti chikv igm human elisa kit
    Comparison of <t>EILV/CHIKV</t> and Abcam human anti-CHIKV <t>IgM-capture</t> <t>ELISA</t> methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.
    Anti Chikv Igm Human Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chikv igm human elisa kit/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chikv igm human elisa kit - by Bioz Stars, 2021-05
    93/100 stars
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    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques:

    Diversity of the antibody response patterns in chikungunya acute febrile versus chronic patients. ( A ) Paired analysis of CHIKV-specific plasma IgM (red circles) and IgG (blue squares) values in individual CHIKV-confirmed patients within the no antibodies, IgM-alone, or IgM and IgG antibody response patterns in the acute febrile (left, n = 133), early chronic (middle, n = 21), and late chronic (right, n = 30) phases. Within each antibody response pattern group, the patients are stratified based on increasing IgM values on the x axis. Horizontal dotted line indicates assay cutoff for IgM and IgG. The samples that were also positive for CHIKV PCR are indicated by green-filled symbols. ( B ) Evaluation of plasma CHIKV NT antibody activity in each of the patient groups that are described in A . Dotted gates were placed to further subgroup the patients based on a combination of IgM, IgG, and NT activity. NT assay limit of detection is indicated by the horizontal dotted line. The NT antibody titers were significantly different between the IgM-alone group and IgM and IgG group in the acute febrile patients. Statistical significance was calculated by unpaired Mann-Whitney U test. ( C ) Relative proportion of the patients with each of the indicated antibody response patterns shown in B among the CHIKV-confirmed patients in febrile phase (left), early chronic phase (middle), and late chronic phase (right).

    Journal: JCI Insight

    Article Title: Antibody response patterns in chikungunya febrile phase predict protection versus progression to chronic arthritis

    doi: 10.1172/jci.insight.130509

    Figure Lengend Snippet: Diversity of the antibody response patterns in chikungunya acute febrile versus chronic patients. ( A ) Paired analysis of CHIKV-specific plasma IgM (red circles) and IgG (blue squares) values in individual CHIKV-confirmed patients within the no antibodies, IgM-alone, or IgM and IgG antibody response patterns in the acute febrile (left, n = 133), early chronic (middle, n = 21), and late chronic (right, n = 30) phases. Within each antibody response pattern group, the patients are stratified based on increasing IgM values on the x axis. Horizontal dotted line indicates assay cutoff for IgM and IgG. The samples that were also positive for CHIKV PCR are indicated by green-filled symbols. ( B ) Evaluation of plasma CHIKV NT antibody activity in each of the patient groups that are described in A . Dotted gates were placed to further subgroup the patients based on a combination of IgM, IgG, and NT activity. NT assay limit of detection is indicated by the horizontal dotted line. The NT antibody titers were significantly different between the IgM-alone group and IgM and IgG group in the acute febrile patients. Statistical significance was calculated by unpaired Mann-Whitney U test. ( C ) Relative proportion of the patients with each of the indicated antibody response patterns shown in B among the CHIKV-confirmed patients in febrile phase (left), early chronic phase (middle), and late chronic phase (right).

    Article Snippet: Serology tests.CHIKV-specific IgM and IgG were detected using chikungunya IgM capture ELISA kits (Abcam ab177848) and chikungunya IgG capture ELISA kit (Abcam ab177835) following the manufacturer’s recommendations.

    Techniques: Polymerase Chain Reaction, Activity Assay, MANN-WHITNEY

    Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Article Snippet: The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA.

    Techniques: Titration

    Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Article Snippet: The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA.

    Techniques: Positive Control

    Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Article Snippet: The Abcam Anti-CHIKV IgM human ELISA kit was evaluated at CDC and CARPHA.

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control