Journal: bioRxiv

Article Title: Improved in situ Characterization of Proteome-wide Protein Complex Dynamics with Thermal Proximity Co-Aggregation

doi: 10.1101/2023.02.13.528386

Figure Lengend Snippet: (a) Dynamics of CORUM-annotated protein complexes under glucose deprivation as profiled by Slim-TPCA. Complexes are grouped based on their functions. (b) K562 cells were incubated in presence and absence of glucose followed by quantification of ATP based on luminescence intensity (n = 3 independent biological experiments). Data are presented as mean values ± s.d. (error bars). (c) K562 cells were incubated in presence and absence of glucose, harvested at different time points and analyzed by immunoblottingusing Phospho-Rb (Ser780) antibody, Phospho-Histone H3(Ser10) antibody, and Phospho-CDK Substrate antibody. GAPDH antibody and β-Tubulin antibody were used as loading control.

Article Snippet: Phospho-Rb (Ser780) antibody, Phospho-Histone H3(Ser10) antibody, Phospho-CDK Substrate antibody, and β-Tubulin antibody were obtained from Cell Signaling Technology (Danvers, USA).

Techniques: Incubation