Journal: Experimental Animals

Article Title: Abietic acid attenuates sepsis-induced lung injury by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to inhibit M1 macrophage polarization

doi: 10.1538/expanim.22-0018

Figure Lengend Snippet: Abietic acid inhibited M1 macrophage polarization in BALF of sepsis mice. (A) Representative flow scatter plot of M1 macrophages (F4/80+CD86+ cells) in BALF detected by flow cytometry. (B) Representative flow scatter plot of M2 macrophages (F4/80+CD163+ cells) in BALF detected by flow cytometry. (C) Statistical comparison of the proportion of M1 macrophages in BALF from 7 mice per group. (D) Statistical comparison of the proportion of M2 macrophages in BALF from 7 mice per group. Statistical comparison of the proportion of M1/M2 macrophages ratio in BALF from 7 mice per group. Data from 7 mice for each group are plotted as (mean ± SD) as a column chart, and P values were calculated by analysis of variance. *** P <0.001 vs. Sham group, ns P >0.05, # P <0.05, ## P <0.01 and ### P <0.001 vs. CLP group.

Article Snippet: Next, cells in BALF were stained with anti-CD86-FITC (99879, Cell Signaling Technology, Boston, MA, USA), anti-CD163-APC (MA5-17717, Invitrogen, Grand Island, NY, USA) and anti-F4/80-PE (80380, Cell Signaling Technology) for 30 min in the dark at 4°C.

Techniques: Flow Cytometry