anti cd8a antibody (Boster Bio)


Structured Review

Anti Cd8a Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd8a antibody/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Targeting complement C5a to improve radiotherapy sensitivity in non-small cell lung cancer"
Article Title: Targeting complement C5a to improve radiotherapy sensitivity in non-small cell lung cancer
Journal: Translational Lung Cancer Research
doi: 10.21037/tlcr-23-258

Figure Legend Snippet: Infiltration of recruited CD8 + T cells increased after treatment of RT. (A) Mice were inoculated subcutaneously with LLC tumor cells and treated with 8 Gy of local RT daily for 3 times. Tumor tissues were collected at the indicated time points for subsequent analysis. (B) Tumor tissues in RT and control groups. (C) Immunofluorescence of tumors in RT and control groups. Sections were stained with an antibody recognizing CD8 (green) and DAPI (blue). Scale bars represent 50 µm. Representative images are shown. (D) Flow cytometry assays displayed that infiltration of CD8 + T cells were upregulated after RT. RT, radiotherapy; DAPI, 4',6-diamidino-2-phenylindole; FSC-A, forward scatter-A; FITC-A, fluorescein isothiocyanate-A; LLC, Lewis lung carcinoma; con, control.
Techniques Used: Immunofluorescence, Staining, Flow Cytometry

Figure Legend Snippet: RT upregulated C5aR1 expression in RT-recruited CD8 + T cells. (A) CD8 + T cells were sorted by magnetic beads for RNA-seq. (B) RNA-seq analysis was displayed and the differential genes are shown in the heat map. (C) DEG counts in RT and control groups. (D) Top 20 of GO enrichment of the up-regulated genes. The red arrows indicate that these functional pathways exist C5aR1 gene enrichment. (E) Volcano map of RNA-seq showing the DEGs. The red dots indicate significantly upregulated genes. RNA-seq, RNA sequencing; RT, radiotherapy; DEGs, differentially expressed genes; GO, Gene Ontology.
Techniques Used: Expressing, Magnetic Beads, RNA Sequencing Assay, Functional Assay
anti cd8 (Boster Bio)


Structured Review
Anti Cd8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd8/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
cd8a (Boster Bio)


Structured Review
Cd8a, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd8a/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
cd8 (Boster Bio)


Structured Review

Cd8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd8/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "CD147 contributes to SARS-CoV-2-induced pulmonary fibrosis"
Article Title: CD147 contributes to SARS-CoV-2-induced pulmonary fibrosis
Journal: Signal Transduction and Targeted Therapy
doi: 10.1038/s41392-022-01230-5

Figure Legend Snippet: hCD147 transgenic mice infected with SARS-CoV-2 and delta variant mimicked pulmonary fibrosis pathology. a hCD147 mice or C57BL/6J mice were inoculated via the intranasal with 3 × 10 5 TCID 50 of SARS-CoV-2 or delta variant, and samples were collected at 2, 6, 13, 20 and 27 d.p.i. b RT-qPCR for viral RNA levels in lung tissues at 2 d.p.i. n = 3 for C57BL/6J group, n = 6 for hCD147 groups. c H&E staining of lung tissue sections from 2 to 27 d.p.i. Scale bars, 50 μm. d TEM analysis of lung samples from 2 to 27 d.p.i. The stars indicate collagen fibrils; the arrows indicate elastic fibers. Black scale bars, 20 μm. Yellow scale bars, 2 μm. e Statistics of the percentage of cells positive for α-SMA (fibroblasts), F4/80 (macrophages), CD3 (T cells), CD4, CD8, CD19 (B cells), Ly6G (neutrophils), and NCR1 (NK cells). n = 12 images for each group, one-way ANOVA followed by multiple comparisons
Techniques Used: Transgenic Assay, Infection, Variant Assay, Quantitative RT-PCR, Staining
rabbit monoclonal anti cd8 antibody (Boster Bio)


Structured Review

Rabbit Monoclonal Anti Cd8 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti cd8 antibody/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in Sarcoma Microenvironment"
Article Title: CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in Sarcoma Microenvironment
Journal: Molecular cancer therapeutics
doi: 10.1158/1535-7163.MCT-20-0591

Figure Legend Snippet: Systemic treatment of PLX3397 depletes TAMs and increases lymphocyte infiltration into LM8 osteosarcoma. A. Composition of tumor cells and TAMs evaluated by flow-cytometric analysis using the dissociated LM8 tumor cells. Data were represented as mean ± SEM; n = 3; Mann–Whitney test: *p < 0.05. B. Flow cytometry analysis of TAMs (CD45+CD11b+CD206+; left) and CD8 T cells (CD45+CD3+CD8+; right) within the dissociated LM8 tumor cells and representative flow data. C. Composition of infiltrating immune cells (CD3+, CD4+, and CD8+ cells) evaluated by flow-cytometric analysis using the dissociated LM8 tumor cells. Data were represented as mean ± SEM; n = 3; Mann – Whitney test: *p < 0.05. D. Composition of infiltrating FOXP3+ regulatory T cells evaluated by immunohistochemistry using the dissociated LM8 tumor cells. Data were represented as mean ± SEM; n = 3; Mann–Whitney test: *p < 0.05. E. Distribution of infiltrating CD68+ macrophages, CD8+ T cells, and FOXP3+ regulatory T cells in PLX3397- or PBS-treated tumors (green). Nuclei were stained with DAPI (blue). Left, H&E staining images. Scale bars, 50 μm (lower) and 10 μm (upper).
Techniques Used: MANN-WHITNEY, Flow Cytometry, Immunohistochemistry, Staining
rabbit monoclonal anti cd8 antibody (Boster Bio)


Structured Review

Rabbit Monoclonal Anti Cd8 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti cd8 antibody/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in Sarcoma Microenvironment"
Article Title: CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in Sarcoma Microenvironment
Journal: Molecular cancer therapeutics
doi: 10.1158/1535-7163.MCT-20-0591

Figure Legend Snippet: Systemic treatment of PLX3397 depletes TAMs and increases lymphocyte infiltration into LM8 osteosarcoma. A. Composition of tumor cells and TAMs evaluated by flow-cytometric analysis using the dissociated LM8 tumor cells. Data were represented as mean ± SEM; n = 3; Mann–Whitney test: *p < 0.05. B. Flow cytometry analysis of TAMs (CD45+CD11b+CD206+; left) and CD8 T cells (CD45+CD3+CD8+; right) within the dissociated LM8 tumor cells and representative flow data. C. Composition of infiltrating immune cells (CD3+, CD4+, and CD8+ cells) evaluated by flow-cytometric analysis using the dissociated LM8 tumor cells. Data were represented as mean ± SEM; n = 3; Mann – Whitney test: *p < 0.05. D. Composition of infiltrating FOXP3+ regulatory T cells evaluated by immunohistochemistry using the dissociated LM8 tumor cells. Data were represented as mean ± SEM; n = 3; Mann–Whitney test: *p < 0.05. E. Distribution of infiltrating CD68+ macrophages, CD8+ T cells, and FOXP3+ regulatory T cells in PLX3397- or PBS-treated tumors (green). Nuclei were stained with DAPI (blue). Left, H&E staining images. Scale bars, 50 μm (lower) and 10 μm (upper).
Techniques Used: MANN-WHITNEY, Flow Cytometry, Immunohistochemistry, Staining
cd8 (Boster Bio)


Structured Review

Cd8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd8/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Fibroblast Common Serum Response Signature-Related Classification Affects the Tumour Microenvironment and Predicts Prognosis in Bladder Cancer"
Article Title: Fibroblast Common Serum Response Signature-Related Classification Affects the Tumour Microenvironment and Predicts Prognosis in Bladder Cancer
Journal: Oxidative Medicine and Cellular Longevity
doi: 10.1155/2022/5645944

Figure Legend Snippet: ANLN to immunity in bladder cancer. (a) Boxplot of the abundance of PD-L1 expression in the CRS groups in the TCGA-BLCA cohort. (b) Correlation analysis between PD-L1 expression and the CRS in TCGA cohort. mRNA levels of (c) ANLN or (d) EML1 in anti-PD-L1 responsiveness in the IMvigor210 cohort. (e, f) Correlation analyses of expression of ANLN and CD8 and PD-L1 in TCGA cohort. (g) Survival probability of patients with differential expression of ANLN of TCGA and GSE13507 cohorts. Levels of ANLN were identified according to the median of ANLN. (h) Correlation analyses of ANLN expression and the CRS. (i) Correlation analyses of expression of ANLN and TNM staging. (j) Correlation analyses of expression of ANLN and grade. Correlation analyses between (k) number of CD8+ T cells or (l) PD-L1 expression and ANLN expression of patients with BLCA in our hospital cohort. (m) Representative IHC or HE images of ANLN, CD8, PD-L1, and Ki67. CR: complete response; PR: partial response; SD: stable disease; PD: progressive disease; CRS: fibroblast common serum response risk score.
Techniques Used: Expressing
anti cd8 ab boster (Boster Bio)


Structured Review
Anti Cd8 Ab Boster, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd8 ab boster/product/Boster Bio
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti cd8 ab (Boster Bio)


Structured Review

Anti Cd8 Ab, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd8 ab/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation"
Article Title: Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation
Journal: Cell Transplantation
doi: 10.1177/09636897211054503

Figure Legend Snippet: The effect of LEF treatment on CMR- and AVR- mediated immune responses. (A) MLR responses. Recipient splenocytes were isolated at POD 6 (responders) and irradiated naïve SD or BALB/c splenocytes (stimulators) were co-cultured for three days. Data are representative of three independent experiments. (B) Absolute numbers of splenocytes in LEF -treated and normal-saline treated mice recipients. Naïve C57BL/6 mice are shown for comparison ( n = 3 mice/group). (C) Representative proportion of CD4 + and CD8 + T cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD4 + and CD8 + T cells was determined by flow cytometry ( n = 3 mice/group). (D) Representative proportion of CD19 + B cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD19 + B cells was determined by flow cytometry ( n = 3 mice/group). (E) The number of CD3 + T cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (F) The number of CD19 + B cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (G) Representative proportion of donor-specific antibodies in recipient serum. Serum was collected from xenograft and allograft recipients at POD 6, and the percent of IgG1, IgG2a, and IgM was determined by flow cytometry ( n = 3 mice/group). (H) Serum levels of proinflammatory cytokines. Peripheral blood was collected at POD 6 and IFN-γ serum levels were measured by ELISA ( n = 3 mice/group). (I) CD3 + T cells were isolated from naïve C57BL/6 mice, and co-cultured with anti-CD3 and anti-CD28 monoclonal antibody in the absence and presence of LEF for 3 days, the supernatant was collected and measured by ELISA ( n = 3 separate experiments). Data are presented as the mean ± SEM of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the normal saline-treated group.
Techniques Used: Isolation, Irradiation, Cell Culture, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and CD8 + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P < 0.05, ** P < 0.01 compared to the normal saline-treated group.
Techniques Used: Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining
anti cd8 (Boster Bio)


Structured Review
Anti Cd8, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd8/product/Boster Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99