anti cd8 alpha cd8a antibody picoband  (Boster Bio)


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    Boster Bio anti cd8 alpha cd8a antibody picoband
    Detection of mitochondrial damage and apoptosis in <t>CD8</t> + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P
    Anti Cd8 Alpha Cd8a Antibody Picoband, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8 alpha cd8a antibody picoband/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 alpha cd8a antibody picoband - by Bioz Stars, 2022-12
    92/100 stars

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    1) Product Images from "Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis"

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    Journal: World Journal of Gastrointestinal Oncology

    doi: 10.4251/wjgo.v14.i6.1124

    Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P
    Figure Legend Snippet: Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P

    Techniques Used: Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P
    Figure Legend Snippet: Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P
    Figure Legend Snippet: Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry

    Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P
    Figure Legend Snippet: Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P

    Techniques Used: Cell Culture, Flow Cytometry

    PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.
    Figure Legend Snippet: PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.

    Techniques Used: Expressing, Double Staining

    Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P
    Figure Legend Snippet: Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

    Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.
    Figure Legend Snippet: Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.

    Techniques Used: Expressing, Cell Culture, Sequencing

    Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.
    Figure Legend Snippet: Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.

    Techniques Used: Transmission Assay, Electron Microscopy, Cell Culture, Co-Culture Assay

    CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.
    Figure Legend Snippet: CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.

    Techniques Used:

    Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P
    Figure Legend Snippet: Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry

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    Boster Bio anti cd8 alpha cd8a antibody picoband
    Detection of mitochondrial damage and apoptosis in <t>CD8</t> + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P
    Anti Cd8 Alpha Cd8a Antibody Picoband, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8 alpha cd8a antibody picoband/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 alpha cd8a antibody picoband - by Bioz Stars, 2022-12
    92/100 stars
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    Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry

    Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Culture, Flow Cytometry

    PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Expressing, Double Staining

    Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

    Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Expressing, Cell Culture, Sequencing

    Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Transmission Assay, Electron Microscopy, Cell Culture, Co-Culture Assay

    CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques:

    Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry

    Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and CD8 + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P

    Journal: Cell Transplantation

    Article Title: Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation

    doi: 10.1177/09636897211054503

    Figure Lengend Snippet: Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and CD8 + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P

    Article Snippet: Immunohistochemical staining was performed as described previously , the sections were then stained with primary antibody anti-CD4 Ab (Boster, Wuhan, China), anti-CD8 Ab (Boster), anti-CD20 Ab (Boster), anti-IgM (Boster), and secondary antibody goat anti-rabbit IgG (GB23303; Boster, Wuhan, China).

    Techniques: Expressing, Quantitative RT-PCR, Mouse Assay, Immunohistochemistry, Staining

    The effect of LEF treatment on CMR- and AVR- mediated immune responses. (A) MLR responses. Recipient splenocytes were isolated at POD 6 (responders) and irradiated naïve SD or BALB/c splenocytes (stimulators) were co-cultured for three days. Data are representative of three independent experiments. (B) Absolute numbers of splenocytes in LEF -treated and normal-saline treated mice recipients. Naïve C57BL/6 mice are shown for comparison ( n = 3 mice/group). (C) Representative proportion of CD4 + and CD8 + T cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD4 + and CD8 + T cells was determined by flow cytometry ( n = 3 mice/group). (D) Representative proportion of CD19 + B cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD19 + B cells was determined by flow cytometry ( n = 3 mice/group). (E) The number of CD3 + T cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (F) The number of CD19 + B cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (G) Representative proportion of donor-specific antibodies in recipient serum. Serum was collected from xenograft and allograft recipients at POD 6, and the percent of IgG1, IgG2a, and IgM was determined by flow cytometry ( n = 3 mice/group). (H) Serum levels of proinflammatory cytokines. Peripheral blood was collected at POD 6 and IFN-γ serum levels were measured by ELISA ( n = 3 mice/group). (I) CD3 + T cells were isolated from naïve C57BL/6 mice, and co-cultured with anti-CD3 and anti-CD28 monoclonal antibody in the absence and presence of LEF for 3 days, the supernatant was collected and measured by ELISA ( n = 3 separate experiments). Data are presented as the mean ± SEM of three independent experiments. * P

    Journal: Cell Transplantation

    Article Title: Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation

    doi: 10.1177/09636897211054503

    Figure Lengend Snippet: The effect of LEF treatment on CMR- and AVR- mediated immune responses. (A) MLR responses. Recipient splenocytes were isolated at POD 6 (responders) and irradiated naïve SD or BALB/c splenocytes (stimulators) were co-cultured for three days. Data are representative of three independent experiments. (B) Absolute numbers of splenocytes in LEF -treated and normal-saline treated mice recipients. Naïve C57BL/6 mice are shown for comparison ( n = 3 mice/group). (C) Representative proportion of CD4 + and CD8 + T cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD4 + and CD8 + T cells was determined by flow cytometry ( n = 3 mice/group). (D) Representative proportion of CD19 + B cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD19 + B cells was determined by flow cytometry ( n = 3 mice/group). (E) The number of CD3 + T cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (F) The number of CD19 + B cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (G) Representative proportion of donor-specific antibodies in recipient serum. Serum was collected from xenograft and allograft recipients at POD 6, and the percent of IgG1, IgG2a, and IgM was determined by flow cytometry ( n = 3 mice/group). (H) Serum levels of proinflammatory cytokines. Peripheral blood was collected at POD 6 and IFN-γ serum levels were measured by ELISA ( n = 3 mice/group). (I) CD3 + T cells were isolated from naïve C57BL/6 mice, and co-cultured with anti-CD3 and anti-CD28 monoclonal antibody in the absence and presence of LEF for 3 days, the supernatant was collected and measured by ELISA ( n = 3 separate experiments). Data are presented as the mean ± SEM of three independent experiments. * P

    Article Snippet: Immunohistochemical staining was performed as described previously , the sections were then stained with primary antibody anti-CD4 Ab (Boster, Wuhan, China), anti-CD8 Ab (Boster), anti-CD20 Ab (Boster), anti-IgM (Boster), and secondary antibody goat anti-rabbit IgG (GB23303; Boster, Wuhan, China).

    Techniques: Isolation, Irradiation, Cell Culture, Mouse Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay