anti cd8 alpha cd8a antibody picoband  (Boster Bio)


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    Boster Bio anti cd8 alpha cd8a antibody picoband
    Detection of mitochondrial damage and apoptosis in <t>CD8</t> + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P
    Anti Cd8 Alpha Cd8a Antibody Picoband, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8 alpha cd8a antibody picoband/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 alpha cd8a antibody picoband - by Bioz Stars, 2022-08
    94/100 stars

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    1) Product Images from "Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis"

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    Journal: World Journal of Gastrointestinal Oncology

    doi: 10.4251/wjgo.v14.i6.1124

    Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P
    Figure Legend Snippet: Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P

    Techniques Used: Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P
    Figure Legend Snippet: Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P
    Figure Legend Snippet: Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry

    Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P
    Figure Legend Snippet: Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P

    Techniques Used: Cell Culture, Flow Cytometry

    PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.
    Figure Legend Snippet: PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.

    Techniques Used: Expressing, Double Staining

    Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P
    Figure Legend Snippet: Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

    Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.
    Figure Legend Snippet: Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.

    Techniques Used: Expressing, Cell Culture, Sequencing

    Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.
    Figure Legend Snippet: Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.

    Techniques Used: Transmission Assay, Electron Microscopy, Cell Culture, Co-Culture Assay

    CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.
    Figure Legend Snippet: CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.

    Techniques Used:

    Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P
    Figure Legend Snippet: Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P

    Techniques Used: Cell Function Assay, Cell Culture, Flow Cytometry

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    Boster Bio anti cd8 ab
    Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and <t>CD8</t> + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P
    Anti Cd8 Ab, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8 ab/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 ab - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    92
    Boster Bio anti cd8 alpha cd8a antibody picoband
    Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and <t>CD8</t> + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P
    Anti Cd8 Alpha Cd8a Antibody Picoband, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8 alpha cd8a antibody picoband/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 alpha cd8a antibody picoband - by Bioz Stars, 2022-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and CD8 + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P

    Journal: Cell Transplantation

    Article Title: Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation

    doi: 10.1177/09636897211054503

    Figure Lengend Snippet: Phenotypic characteristics of grafts. Xenografts and allografts were recovered at POD 6. Relative mRNA expression of IL-2, IFN-γ, IL-4, and TNF-α in (A) xenograft and (B) allografts measured by qRT-PCR ( n = 3 mice/group). (C) Immunohistochemistry staining of CD20 + (brown) in xenograft and allograft; bar indicates 20 µm and quantified. (D) IgM in grafts were examined by immunohistochemistry staining; bar indicates 20 µm and quantified. Immunohistochemistry staining of CD4 + (brown), and CD8 + (brown) in (E) xenograft and (H) allografts. (Original magnification: ×400). Data are presented as mean ± SEM from three independent experiments. * P

    Article Snippet: Immunohistochemical staining was performed as described previously , the sections were then stained with primary antibody anti-CD4 Ab (Boster, Wuhan, China), anti-CD8 Ab (Boster), anti-CD20 Ab (Boster), anti-IgM (Boster), and secondary antibody goat anti-rabbit IgG (GB23303; Boster, Wuhan, China).

    Techniques: Expressing, Quantitative RT-PCR, Mouse Assay, Immunohistochemistry, Staining

    The effect of LEF treatment on CMR- and AVR- mediated immune responses. (A) MLR responses. Recipient splenocytes were isolated at POD 6 (responders) and irradiated naïve SD or BALB/c splenocytes (stimulators) were co-cultured for three days. Data are representative of three independent experiments. (B) Absolute numbers of splenocytes in LEF -treated and normal-saline treated mice recipients. Naïve C57BL/6 mice are shown for comparison ( n = 3 mice/group). (C) Representative proportion of CD4 + and CD8 + T cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD4 + and CD8 + T cells was determined by flow cytometry ( n = 3 mice/group). (D) Representative proportion of CD19 + B cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD19 + B cells was determined by flow cytometry ( n = 3 mice/group). (E) The number of CD3 + T cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (F) The number of CD19 + B cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (G) Representative proportion of donor-specific antibodies in recipient serum. Serum was collected from xenograft and allograft recipients at POD 6, and the percent of IgG1, IgG2a, and IgM was determined by flow cytometry ( n = 3 mice/group). (H) Serum levels of proinflammatory cytokines. Peripheral blood was collected at POD 6 and IFN-γ serum levels were measured by ELISA ( n = 3 mice/group). (I) CD3 + T cells were isolated from naïve C57BL/6 mice, and co-cultured with anti-CD3 and anti-CD28 monoclonal antibody in the absence and presence of LEF for 3 days, the supernatant was collected and measured by ELISA ( n = 3 separate experiments). Data are presented as the mean ± SEM of three independent experiments. * P

    Journal: Cell Transplantation

    Article Title: Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation

    doi: 10.1177/09636897211054503

    Figure Lengend Snippet: The effect of LEF treatment on CMR- and AVR- mediated immune responses. (A) MLR responses. Recipient splenocytes were isolated at POD 6 (responders) and irradiated naïve SD or BALB/c splenocytes (stimulators) were co-cultured for three days. Data are representative of three independent experiments. (B) Absolute numbers of splenocytes in LEF -treated and normal-saline treated mice recipients. Naïve C57BL/6 mice are shown for comparison ( n = 3 mice/group). (C) Representative proportion of CD4 + and CD8 + T cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD4 + and CD8 + T cells was determined by flow cytometry ( n = 3 mice/group). (D) Representative proportion of CD19 + B cells in recipient splenocytes. A total of 1 × 10 6 splenocytes were isolated at POD 6, and the percentage of CD19 + B cells was determined by flow cytometry ( n = 3 mice/group). (E) The number of CD3 + T cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (F) The number of CD19 + B cells in recipient spleen were determined by flow cytometry ( n = 3 mice/group). (G) Representative proportion of donor-specific antibodies in recipient serum. Serum was collected from xenograft and allograft recipients at POD 6, and the percent of IgG1, IgG2a, and IgM was determined by flow cytometry ( n = 3 mice/group). (H) Serum levels of proinflammatory cytokines. Peripheral blood was collected at POD 6 and IFN-γ serum levels were measured by ELISA ( n = 3 mice/group). (I) CD3 + T cells were isolated from naïve C57BL/6 mice, and co-cultured with anti-CD3 and anti-CD28 monoclonal antibody in the absence and presence of LEF for 3 days, the supernatant was collected and measured by ELISA ( n = 3 separate experiments). Data are presented as the mean ± SEM of three independent experiments. * P

    Article Snippet: Immunohistochemical staining was performed as described previously , the sections were then stained with primary antibody anti-CD4 Ab (Boster, Wuhan, China), anti-CD8 Ab (Boster), anti-CD20 Ab (Boster), anti-IgM (Boster), and secondary antibody goat anti-rabbit IgG (GB23303; Boster, Wuhan, China).

    Techniques: Isolation, Irradiation, Cell Culture, Mouse Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Detection of mitochondrial damage and apoptosis in CD8 + T cells. Activated CD8 + T cells, co-cultured with Huh-7 cells for 3 d, were detected by flow cytometry. A: JC-1; B: Reactive oxygen species; C: Apoptosis. The data represent the mean ± SE; b P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Regulation of CD8 + T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8 + T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; d P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry

    Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Regulation of CD8 + T cell function by Mdivi-1. A-C: Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing 5 μmol/L Mdivi-1 for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; c P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry

    Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Effect of glutamine deprivation on CD8 + T cells co-cultured with hepatoma cells. Activated CD8 + T cells were co-cultured with Huh-7 cells in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; a P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Culture, Flow Cytometry

    PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: PD-1 expression by infiltrating CD8 + T cells in liver tissues. Tissues were subjected to fluorescent double staining with anti-CD8 and anti-PD-1 antibodies. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 400 μm.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Expressing, Double Staining

    Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Evaluations of CD8 + T cell function. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Flow cytometry was used to detect the expression of indicated molecules in CD8 + T cells. A: PD-1; B: PRF1; C: Granzyme B (GZMB). D and E: Quantitative real-time polymerase chain reaction was used to detect the mRNA levels in CD8 + T cells (D: PRF1 ; E: GZMB ). The data represent the mean ± SE; a P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

    Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Differential gene expression in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. Total RNA was extracted from CD8 + T cells for transcriptome sequencing. A: GO enrichment plot of upregulated genes; B: GO enrichment plot of downregulated genes.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Expressing, Cell Culture, Sequencing

    Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Transmission electron microscopy images of mitochondria in CD8 + T cells. Activated CD8 + T cells were co-cultured with Huh-7 cells for 3 d. The morphology of mitochondria in CD8 + T cells was detected by transmission electron microscopy. A: Control; B: Co-culture. Scale bar: 2 μm.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Transmission Assay, Electron Microscopy, Cell Culture, Co-Culture Assay

    CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: CD8 + T cell infiltration in liver tissues. A: Hepatocellular carcinoma tissues; B: Paracancerous tissues. Scale bar: 200 μm.

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques:

    Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P

    Journal: World Journal of Gastrointestinal Oncology

    Article Title: Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

    doi: 10.4251/wjgo.v14.i6.1124

    Figure Lengend Snippet: Altered CD8 + T cell function by glutamine deficiency. Activated CD8 + T cells were cultured in RPMI 1640 containing no glutamine (0Gln) for 3 d. The levels of indicated molecules in CD8 + T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B; D: JC-1; E: Reactive oxygen species; F: Apoptosis. The data represent the mean ± SE; b P

    Article Snippet: Immunofluorescence staining of paraffin-embedded human liver tissue sections was performed using anti-PD-1/CD279 (66220-1-Ig, Proteintech, Rosemont, IL, United States) and anti-CD8α (PB9249, Boster Bio, Pleasanton, CA, United States) antibodies.

    Techniques: Cell Function Assay, Cell Culture, Flow Cytometry