anti cd40  (Sino Biological)


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    Name:
    Anti CD40 Ligand Antibody Mouse Monoclonal
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human CD40L CD154 TNFSF5 rh CD40L CD154 TNFSF5 Catalog 10239 H08E NP 000065 1 Glu 108 Leu 261 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    10239-MM04
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Human
    Applications:
    ELISA
    Immunogen:
    Recombinant Human CD40L / CD154 / TNFSF5 protein (Catalog#10239-H08E)
    Product Aliases:
    Anti-CD154 Antibody, Anti-CD40 Ligand Antibody, Anti-CD40L Antibody, Anti-gp39 Antibody, Anti-hCD40L Antibody, Anti-HIGM1 Antibody, Anti-IGM Antibody, Anti-IMD3 Antibody, Anti-T-BAM Antibody, Anti-TNFSF5 Antibody, Anti-TRAP Antibody
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Sino Biological anti cd40
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human CD40L CD154 TNFSF5 rh CD40L CD154 TNFSF5 Catalog 10239 H08E NP 000065 1 Glu 108 Leu 261 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/anti cd40/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd40 - by Bioz Stars, 2021-07
    92/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes
    Article Snippet: The generated rAds were used to infect confluent HeLa cells in six-well plate at a multiplicity of infection of 100. .. Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China). ..

    Infection:

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes
    Article Snippet: The generated rAds were used to infect confluent HeLa cells in six-well plate at a multiplicity of infection of 100. .. Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China). ..

    Western Blot:

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes
    Article Snippet: The generated rAds were used to infect confluent HeLa cells in six-well plate at a multiplicity of infection of 100. .. Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes
    Article Snippet: The generated rAds were used to infect confluent HeLa cells in six-well plate at a multiplicity of infection of 100. .. Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China). ..

    Isolation:

    Article Title: Chimeric specific antigen epitope-carrying dendritic cells induce interleukin-17(+) regulatory T cells to suppress food allergy.
    Article Snippet: The on-purpose-modulated dendritic cells (DCs) have shown charming effects on restoring immune regulatory functions in subjects with immune diseases. .. The on-purpose-modulated dendritic cells (DCs) have shown charming effects on restoring immune regulatory functions in subjects with immune diseases. ..

    Cell Culture:

    Article Title: Chimeric specific antigen epitope-carrying dendritic cells induce interleukin-17(+) regulatory T cells to suppress food allergy.
    Article Snippet: The on-purpose-modulated dendritic cells (DCs) have shown charming effects on restoring immune regulatory functions in subjects with immune diseases. .. The on-purpose-modulated dendritic cells (DCs) have shown charming effects on restoring immune regulatory functions in subjects with immune diseases. ..

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  • 92
    Sino Biological anti cd40l monoclonal abs
    Targeting wild-type or codon-optimized HA2 via <t>CD40L</t> provides complete heterosubtypic protection. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Data are shown from one experiment with n =10 mice per treatment group.
    Anti Cd40l Monoclonal Abs, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd40l monoclonal abs/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd40l monoclonal abs - by Bioz Stars, 2021-07
    92/100 stars
      Buy from Supplier

    92
    Sino Biological antibody drug
    Targeting wild-type or codon-optimized HA2 via <t>CD40L</t> provides complete heterosubtypic protection. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Data are shown from one experiment with n =10 mice per treatment group.
    Antibody Drug, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody drug/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody drug - by Bioz Stars, 2021-07
    92/100 stars
      Buy from Supplier

    90
    Sino Biological anti cd40
    Targeting wild-type or codon-optimized HA2 via <t>CD40L</t> provides complete heterosubtypic protection. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Data are shown from one experiment with n =10 mice per treatment group.
    Anti Cd40, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd40/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd40 - by Bioz Stars, 2021-07
    90/100 stars
      Buy from Supplier

    N/A
    Produced in rabbits immunized with purified recombinant Rat CD40 TNFRSF5 Catalog 80151 R08H Q4QQW2 RAT Met1 Arg193 Total IgG was purified by Protein A affinity chromatography
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    Image Search Results


    Targeting wild-type or codon-optimized HA2 via CD40L provides complete heterosubtypic protection. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Data are shown from one experiment with n =10 mice per treatment group.

    Journal: Mucosal Immunology

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes

    doi: 10.1038/mi.2014.59

    Figure Lengend Snippet: Targeting wild-type or codon-optimized HA2 via CD40L provides complete heterosubtypic protection. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Data are shown from one experiment with n =10 mice per treatment group.

    Article Snippet: Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China).

    Techniques: Mouse Assay, Construct

    CD40L and HA2 codon-optimization enhance circulating and nasal anti-HA2 antibody response. HA2-specific antibody titers at 2 weeks after ( a ) priming and ( b ) boosting are shown for circulating IgG1 and IgG2a, and nasal IgA. Balb/c mice were intranasally immunized with 10 9 pfu of each rAd virus or with PBS on day 0 and boosted 2 weeks post primary immunization. Data are shown as mean titer±s.d. from one experiment out of three independent experiments, with n =3 mice per treatment group in each experiment. **** P

    Journal: Mucosal Immunology

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes

    doi: 10.1038/mi.2014.59

    Figure Lengend Snippet: CD40L and HA2 codon-optimization enhance circulating and nasal anti-HA2 antibody response. HA2-specific antibody titers at 2 weeks after ( a ) priming and ( b ) boosting are shown for circulating IgG1 and IgG2a, and nasal IgA. Balb/c mice were intranasally immunized with 10 9 pfu of each rAd virus or with PBS on day 0 and boosted 2 weeks post primary immunization. Data are shown as mean titer±s.d. from one experiment out of three independent experiments, with n =3 mice per treatment group in each experiment. **** P

    Article Snippet: Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China).

    Techniques: Mouse Assay

    Enhanced protection and immune response is dependent on CD40L. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Circulating HA2-specific IgG1 and IgG2a titers ( d ) and IFN-γ and IL-4T cell responses ( e ) are shown at 2 weeks post secondary immunization. Data are shown from one experiment with n =8 mice per treatment group in ( a–c ). Data in ( d ) are shown as mean titer±s.d. from one experiment, with n =5 mice per treatment group. Data in ( e ) are shown as mean numbers of spot-forming cells (SFCs)±s.d. from one experiment with n =5. Each sample was tested in triplicates. **** P

    Journal: Mucosal Immunology

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes

    doi: 10.1038/mi.2014.59

    Figure Lengend Snippet: Enhanced protection and immune response is dependent on CD40L. Survival curves and body weight loss of Balb/c mice immunized with two doses of 10 9 pfu of the indicated rAd constructs and challenged with 5 × LD 50 of mouse-adapted influenza ( a ) A/Puerto Rico/8/1934(H1N1) ( b ) A/Jingke/30/95(H3N2) or ( c ) A/chicken/Jiangsu/7/2002(H9N2) viruses 2 weeks post secondary immunization. Circulating HA2-specific IgG1 and IgG2a titers ( d ) and IFN-γ and IL-4T cell responses ( e ) are shown at 2 weeks post secondary immunization. Data are shown from one experiment with n =8 mice per treatment group in ( a–c ). Data in ( d ) are shown as mean titer±s.d. from one experiment, with n =5 mice per treatment group. Data in ( e ) are shown as mean numbers of spot-forming cells (SFCs)±s.d. from one experiment with n =5. Each sample was tested in triplicates. **** P

    Article Snippet: Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China).

    Techniques: Mouse Assay, Construct

    CD40L enhances T-cell responses to HA2. Balb/c mice were intranasally immunized twice with 10 9 pfu of each rAd virus or with PBS. Mice were sacrificed at 2 weeks ( a ) post primary or ( b ) secondary immunization and splenocytes were isolated for IFN-γ, TNF-α, and IL-4 measurement by ELISpot. Data shown as mean numbers of spot-forming cells (SFCs)±s.d. from one experiment with n =3 per treatment group. Each sample was tested in triplicates. **** P

    Journal: Mucosal Immunology

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes

    doi: 10.1038/mi.2014.59

    Figure Lengend Snippet: CD40L enhances T-cell responses to HA2. Balb/c mice were intranasally immunized twice with 10 9 pfu of each rAd virus or with PBS. Mice were sacrificed at 2 weeks ( a ) post primary or ( b ) secondary immunization and splenocytes were isolated for IFN-γ, TNF-α, and IL-4 measurement by ELISpot. Data shown as mean numbers of spot-forming cells (SFCs)±s.d. from one experiment with n =3 per treatment group. Each sample was tested in triplicates. **** P

    Article Snippet: Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China).

    Techniques: Mouse Assay, Isolation, Enzyme-linked Immunospot

    Recombinant Ad constructs and in vitro protein expression. ( a ) Schematic representation of the generated rAd constructs. The rAd-SHA2(Opt)FCD40L and rAd-SHA2(wt)FCD40L were generated to express secreted HA2(Opt)-FCD40L and HA2(wt)-FCD40L fusion proteins, respectively. S is an N-terminal leader sequence derived from human tyrosinase signal peptide. HA2 is the HA2 subunit from influenza A/California/7/2009 virus(H1N1). F is a 27 amino-acid fragment from the T4 bacteriophage fibritin trimerization motif fused with ectodomain (144 amino acids) of the mouse CD40L. The rAd-SHA2(Opt)F expresses secreted trimeric HA2 protein. The rAd-SHA2(wt) and rAd-SHA2(Opt) express secreted monomeric HA2(wt) and HA2(Opt), respectively. The rAd-control is an “empty” control vector. Constructs were engineered in recombinant ΔE1/E3 adenovirus pAdxsi vectors under the control of CMV promoter. ( b ) In vitro protein expression in cell culture. Confluent HeLa cells were infected with rAds at multiplicity of infection of 100 and 48 h later, cell lysates were collected. Protein expression was confirmed by western blot using anti-HA2 polyclonal antibodies.

    Journal: Mucosal Immunology

    Article Title: Targeting the HA2 subunit of influenza A virus hemagglutinin via CD40L provides universal protection against diverse subtypes

    doi: 10.1038/mi.2014.59

    Figure Lengend Snippet: Recombinant Ad constructs and in vitro protein expression. ( a ) Schematic representation of the generated rAd constructs. The rAd-SHA2(Opt)FCD40L and rAd-SHA2(wt)FCD40L were generated to express secreted HA2(Opt)-FCD40L and HA2(wt)-FCD40L fusion proteins, respectively. S is an N-terminal leader sequence derived from human tyrosinase signal peptide. HA2 is the HA2 subunit from influenza A/California/7/2009 virus(H1N1). F is a 27 amino-acid fragment from the T4 bacteriophage fibritin trimerization motif fused with ectodomain (144 amino acids) of the mouse CD40L. The rAd-SHA2(Opt)F expresses secreted trimeric HA2 protein. The rAd-SHA2(wt) and rAd-SHA2(Opt) express secreted monomeric HA2(wt) and HA2(Opt), respectively. The rAd-control is an “empty” control vector. Constructs were engineered in recombinant ΔE1/E3 adenovirus pAdxsi vectors under the control of CMV promoter. ( b ) In vitro protein expression in cell culture. Confluent HeLa cells were infected with rAds at multiplicity of infection of 100 and 48 h later, cell lysates were collected. Protein expression was confirmed by western blot using anti-HA2 polyclonal antibodies.

    Article Snippet: Forty-eight hours later, protein expression in cell lysates of infected cells was confirmed by western blot of proteins separated on sodium dodecyl sulfate -polyacrylamide gel electrophoresis or native gradient (4–12%) polyacrylamide gel electrophoresis as previously described using anti-CD40L monoclonal Abs and rabbit anti-HA Abs (Sino Biological Inc., Beijing, China).

    Techniques: Recombinant, Construct, In Vitro, Expressing, Generated, Sequencing, Derivative Assay, Plasmid Preparation, Cell Culture, Infection, Western Blot