anti cd336 apc  (BioLegend)

 
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    Name:
    APC anti human CD336 NKp44
    Description:
    APC anti human CD336 NKp44 P44 8 Isotype Mouse IgG1 κ Reactivity Human Apps FC Size 25 tests
    Catalog Number:
    325109
    Price:
    125
    Category:
    Human Immunology
    Source:
    Mouse
    Applications:
    FC
    Conjugate:
    APC
    Immunogen:
    recombinant human NKp44
    Size:
    25 tests
    Quantity:
    1
    Preparation:
    The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions The solution is free of unconjugated APC and unconjugated antibody
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    Structured Review

    BioLegend anti cd336 apc
    APC anti human CD336 NKp44
    APC anti human CD336 NKp44 P44 8 Isotype Mouse IgG1 κ Reactivity Human Apps FC Size 25 tests
    https://www.bioz.com/result/anti cd336 apc/product/BioLegend
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd336 apc - by Bioz Stars, 2021-07
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Transient Expression of GATA3 in Hematopoietic Stem Cells Facilitates Helper Innate Lymphoid Cell Differentiation
    Article Snippet: Moreover, viability of cells was analyzed using flow cytometry with the help of fixable viability dye eFluor 780 (eBioscience). .. Expression of surface receptor was determined using the following monoclonal antibodies: anti-CD3-PerCp5.5 (clone HIT3a), anti-CD11c-PerCp5.5 (clone Bu15), anti-D14-PerCp5.5 (clone 63D3), anti-CD19-PerCp5.5 (clone HIB19), anti-CD123-PerCp5.5 (clone 6H6), anti-CD127-PE (clone A019D5), anti-CD294-BV421/BV605 (clone BM16), anti-CD336-APC (clone P44-8), and anti-mouse IgG-FITC/PE (clone Poly4053) (all from Biolegend); anti-CD11a-FITC (clone G43-25B), anti-CD34-PE/APC (clone 4H11), anti-CD45-APC/PE (clone HI30), anti-CD56-BV421/BV605 (clone NCAM16.2), anti-CD94-PerCp5.5/FITC (clone HP-3D9), anti-CD117-PE-Cy7 (clone 104D2), and anti-CD161-PE (clone DX12) (all from BD Biosciences); and anti-α4β7 (Cat#11718, from NIH). ..

    Article Title: Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage
    Article Snippet: Flow cytometry-based viability assessment was performed using the fixable viability dye eFluor 780 (eBioscience). .. Expression of surface receptors was determined using the following monoclonal antibodies: anti-CD3-PerCp5.5 (clone HIT3a), anti-CD10-FITC (clone HI10a), anti-CD11c-PerCp5.5 (clone Bu15), anti-CD15- APC/BV605 (clone W6D3), anti-CD16-FITC (clone 3G8), anti-CD19-PerCp5.5 (clone HIB19), anti-CD38-PE (clone HIT2), anti-CD43-PE (clone CD43–10G7), anti-CD66b-PECy7 (clone G10F5), anti-CD127-PE (clone A019D5), anti-CD336-APC (clone P44–8) and anti-mouse IgG-FITC/PE (clone Poly4053) (all from Biolegend); anti-CD11a-FITC (clone G43–25B), anti-CD11b-FITC/PE (clone M1/70), anti-CD18-APC (clone 6.7), anti-CD34-PE/APC (clone 4H11), anti-CD45-APC/PE (clone HI30), anti-CD45RO-PerCp5.5 (clone UCHL1), anti-CD56-BV421/BV605 (clone NCAM16.2), anti-CD94-PerCp5.5/FITC (clone HP-3D9) and anti-CD123-FITC (clone 7G3) (all from BD Biosciences); anti-PRLR-PE/APC (clone B6.2 + PRLR742), from Novus Biosciences); anti-CD45RA-PerCp5.5 (clone HI100, from Tonbo Biosciences); and anti-α4β7 (Cat# 11718, from NIH AIDS reagent program). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Transient Expression of GATA3 in Hematopoietic Stem Cells Facilitates Helper Innate Lymphoid Cell Differentiation
    Article Snippet: Moreover, viability of cells was analyzed using flow cytometry with the help of fixable viability dye eFluor 780 (eBioscience). .. Expression of surface receptor was determined using the following monoclonal antibodies: anti-CD3-PerCp5.5 (clone HIT3a), anti-CD11c-PerCp5.5 (clone Bu15), anti-D14-PerCp5.5 (clone 63D3), anti-CD19-PerCp5.5 (clone HIB19), anti-CD123-PerCp5.5 (clone 6H6), anti-CD127-PE (clone A019D5), anti-CD294-BV421/BV605 (clone BM16), anti-CD336-APC (clone P44-8), and anti-mouse IgG-FITC/PE (clone Poly4053) (all from Biolegend); anti-CD11a-FITC (clone G43-25B), anti-CD34-PE/APC (clone 4H11), anti-CD45-APC/PE (clone HI30), anti-CD56-BV421/BV605 (clone NCAM16.2), anti-CD94-PerCp5.5/FITC (clone HP-3D9), anti-CD117-PE-Cy7 (clone 104D2), and anti-CD161-PE (clone DX12) (all from BD Biosciences); and anti-α4β7 (Cat#11718, from NIH). ..

    Purification:

    Article Title: Influenza virus uses its neuraminidase protein to evade the recognition of two activating NK cell receptors
    Article Snippet: The human influenza virus A/Puerto Rico/8/34 H1N1 used in this study was generated as previously described [ ]. .. The monoclonal antibodies (mAbs) used in the present study included the anti-Influenza type A monoclonal mAb (Centers for Disease Control Atlanta Georgia), anti-influenza virus type A (H1) mAb (H17-L2) (the kind gift of Dr. Jonathan Yewdell, NIH), APC conjugated anti-human NKp44 mAb (BioLegend), LEAF Purified anti-human CD336 (NKp44), PE- conjugated anti-human NKp46 (Beckman Coulter) and PE-conjugated anti mouse Ncr1 (R & D systems). .. Biotin-SP-AffiniPure Rabbit Anti-Human IgG and anti human Fcγ polyclonal antibodies were purchased from Jackson ImmunoResearch.

    Staining:

    Article Title: Quantification and role of innate lymphoid cell subsets in Chronic Obstructive Pulmonary Disease
    Article Snippet: To exclude lineage markers, the following human monoclonal antibodies were used: peridinin chlorophyll protein–cyanine 5.5 (PerCP)‐conjugated anti‐CD1a (HI49), anti‐CD3 (OKT3), anti‐CD14 (63D3), anti‐CD19 (HIB19), anti‐CD11c (3.9), anti‐CD11b (M1/70), anti‐CD123 (6H6), anti‐CD34 (581), anti‐TCRα/β (IP26), anti‐TCRγ/δ (B1), anti‐BDCA2 (201A), anti‐FCεR1 (AER‐37) and anti‐CD235A (HI264). .. To distinguish ILC subsets based on surface staining, the following antibodies were used: fluorescein isothiocyanate (FITC)‐conjugated anti‐CD45 (HI30), allophycocyanin (APC)‐conjugated anti‐NKp44 (P44‐8), phycoerythrin (PE)/indotricarbocya nine (Cy7)‐conjugated anti‐CD117 (104D2), Brilliant Violet 421TM‐conjugated anti‐CD127 (A019D5), Brilliant Violet 605TM‐conjugated anti‐CD56 (HCD56) (all from BioLegend, San Diego, CA), PE‐conjugated CD161 (HP3610, eBioscience) and biotinylated anti‐CRTH2 (BM19; Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with streptavidin‐APC‐Cy7 (BD Biosciences, San Jose, CA). .. To identify ILC subsets based on cytokine staining, the same PerCP‐conjugated lineage antibodies were used, except for CD3, which was replaced by APC‐Cy7‐conjugated anti‐CD3 (SK7).

    Article Title: HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition
    Article Snippet: Mononuclear cells were isolated from rectal biopsies and 3 × 106 cells were used for phenotypic characterization. .. Flow cytometry staining was carried out for cell surface and intracellular molecules using anti-human fluorochrome-conjugated monoclonal antibodies that are known to cross-react with rhesus macaques: Alexa-Fluor 700 anti-CD3 (SP34-2; 557917, 0.2 mg ml−1 ); PE-C594 anti-IFN-γ (B27; 562392, 5 µl); PerCPCy5.5 anti-CD4 (L200; 552838, 5 µl) from BD Biosciences; V450 anti-TNF-α (Mab11; 502920, 5 µl); NKp44 (P44-8; 325110, 5 µl); PE-Cy7 anti-IL-2 (17H12; 500325, 50 µg ml−1 ); 605NC anti-CD20 (2H7; 302334, 50 µg ml−1 ) from BioLegend; PE-APC eFlour 780 anti-IL-17 (eBio64DEC17; 47–7179, 0.125 mg); FITC anti-CD107a (eBioH4A3; 11-1079-42, 5 µl, 0.5 µg); 605NC anti-CD8 (RPA-T8; 93–0088, 5 µl) from eBioscience; and PE anti-NKG2A (Z199; PN , 5 µl) from Beckman Coulter. .. The yellow and aqua LIVE/DEAD viability dyes (Invitrogen; , 1 µl) were used to exclude dead cells.

    Article Title: Alteration of circulating innate lymphoid cells in patients with atherosclerotic cerebral infarction
    Article Snippet: All samples were treated with sodium heparin and examined within 4 h. Peripheral blood mononuclear cells (PBMCs) were prepared using the Ficoll density gradient for flow cytometry (FCM) and reverse transcription-polymerase chain reaction (RT-PCR). .. For analyzing regulatory T cells, cell surface staining was performed using fluorescein isothiocyanate-conjugated anti-CD45 (HI30 clone, Biolegend, CA, USA); phycoerythrin (PE)-conjugated anti-CD3 (B1.49.9 clone, Biolegend); PE-anti-CD19, CD20, CD14, CD16, CD56, CD11b, CD11c, CD123, and FcγRIa; PerCP-Cy5.5-anti-CD127; PE-cy7-conjugated anti-CD294 (CRTH2, BM16 clone, Biolegend); Brilliant Violet 421 (BV421)-anti-CD117, Alexa Fluor 700-anti-CD161, and APC anti-CD336 (NKp44, Biolegend); and appropriate isotype controls for 20 min at room temperature in the dark, followed by washing with phosphate-buffered saline solution. .. Stained cells were assessed by FCM using an FACS Aria II flow cytometer with BD FACSDiva Software (Becton Dickinson, CA, USA).

    Lysis:

    Article Title: Comprehensive analysis of the percentage of surface receptors and cytotoxic granules positive natural killer cells in patients with pancreatic cancer, gastric cancer, and colorectal cancer
    Article Snippet: Reagents The anti-human CD3-FITC/CD16 + 56-PE mixed antibody was obtained from Beckman Coulter (Brea, CA, USA). .. The anti-human CD3-FITC, CD16-PE/Cy7, CD56-PE/Cy7, NKG2D-PE/Cy7, NKp44-APC, NKp46-PE/Cy7, NKp30-APC, KIR3DL1-PE, DNAM-1-Alexa Fluor 647, and perforin-PerCP/Cy5.5 antibodies, and the RBC Lysis Buffer, Fixation Buffer and Wash Buffer were purchased from Biolegend (San Diego, CA, USA), as well as FITC, PE, PE/Cy7, APC, PerCP, Alexa Fluor-647, and PerCP/Cy 5.5 mouse IgG1 antibodies. .. The anti-human NKG2A-PerCP and granzyme B-APC antibodies were obtained from R & D Systems (Minneapolis, MI, UAS).

    Flow Cytometry:

    Article Title: HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition
    Article Snippet: Mononuclear cells were isolated from rectal biopsies and 3 × 106 cells were used for phenotypic characterization. .. Flow cytometry staining was carried out for cell surface and intracellular molecules using anti-human fluorochrome-conjugated monoclonal antibodies that are known to cross-react with rhesus macaques: Alexa-Fluor 700 anti-CD3 (SP34-2; 557917, 0.2 mg ml−1 ); PE-C594 anti-IFN-γ (B27; 562392, 5 µl); PerCPCy5.5 anti-CD4 (L200; 552838, 5 µl) from BD Biosciences; V450 anti-TNF-α (Mab11; 502920, 5 µl); NKp44 (P44-8; 325110, 5 µl); PE-Cy7 anti-IL-2 (17H12; 500325, 50 µg ml−1 ); 605NC anti-CD20 (2H7; 302334, 50 µg ml−1 ) from BioLegend; PE-APC eFlour 780 anti-IL-17 (eBio64DEC17; 47–7179, 0.125 mg); FITC anti-CD107a (eBioH4A3; 11-1079-42, 5 µl, 0.5 µg); 605NC anti-CD8 (RPA-T8; 93–0088, 5 µl) from eBioscience; and PE anti-NKG2A (Z199; PN , 5 µl) from Beckman Coulter. .. The yellow and aqua LIVE/DEAD viability dyes (Invitrogen; , 1 µl) were used to exclude dead cells.

    Cytometry:

    Article Title: HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition
    Article Snippet: Mononuclear cells were isolated from rectal biopsies and 3 × 106 cells were used for phenotypic characterization. .. Flow cytometry staining was carried out for cell surface and intracellular molecules using anti-human fluorochrome-conjugated monoclonal antibodies that are known to cross-react with rhesus macaques: Alexa-Fluor 700 anti-CD3 (SP34-2; 557917, 0.2 mg ml−1 ); PE-C594 anti-IFN-γ (B27; 562392, 5 µl); PerCPCy5.5 anti-CD4 (L200; 552838, 5 µl) from BD Biosciences; V450 anti-TNF-α (Mab11; 502920, 5 µl); NKp44 (P44-8; 325110, 5 µl); PE-Cy7 anti-IL-2 (17H12; 500325, 50 µg ml−1 ); 605NC anti-CD20 (2H7; 302334, 50 µg ml−1 ) from BioLegend; PE-APC eFlour 780 anti-IL-17 (eBio64DEC17; 47–7179, 0.125 mg); FITC anti-CD107a (eBioH4A3; 11-1079-42, 5 µl, 0.5 µg); 605NC anti-CD8 (RPA-T8; 93–0088, 5 µl) from eBioscience; and PE anti-NKG2A (Z199; PN , 5 µl) from Beckman Coulter. .. The yellow and aqua LIVE/DEAD viability dyes (Invitrogen; , 1 µl) were used to exclude dead cells.

    Recombinase Polymerase Amplification:

    Article Title: HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition
    Article Snippet: Mononuclear cells were isolated from rectal biopsies and 3 × 106 cells were used for phenotypic characterization. .. Flow cytometry staining was carried out for cell surface and intracellular molecules using anti-human fluorochrome-conjugated monoclonal antibodies that are known to cross-react with rhesus macaques: Alexa-Fluor 700 anti-CD3 (SP34-2; 557917, 0.2 mg ml−1 ); PE-C594 anti-IFN-γ (B27; 562392, 5 µl); PerCPCy5.5 anti-CD4 (L200; 552838, 5 µl) from BD Biosciences; V450 anti-TNF-α (Mab11; 502920, 5 µl); NKp44 (P44-8; 325110, 5 µl); PE-Cy7 anti-IL-2 (17H12; 500325, 50 µg ml−1 ); 605NC anti-CD20 (2H7; 302334, 50 µg ml−1 ) from BioLegend; PE-APC eFlour 780 anti-IL-17 (eBio64DEC17; 47–7179, 0.125 mg); FITC anti-CD107a (eBioH4A3; 11-1079-42, 5 µl, 0.5 µg); 605NC anti-CD8 (RPA-T8; 93–0088, 5 µl) from eBioscience; and PE anti-NKG2A (Z199; PN , 5 µl) from Beckman Coulter. .. The yellow and aqua LIVE/DEAD viability dyes (Invitrogen; , 1 µl) were used to exclude dead cells.

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  • 94
    BioLegend allophycocyanin apc conjugated anti nkp44 p44 8
    Quantification of pulmonary ILC in COPD patients and correlation with clinical parameters. For flow cytometric analysis, ILCs were surface‐stained and gated as live, CD45 + lymphocytes that are Lin − (CD3, CD19, CD11c, CD11b, CD1a, CD14, CD34, CD123, TCRαβ, TCRγδ, BDCA2, CD235A and FcεR1) CD161 + CD127 + . ILC subsets were further defined as CD117 − CRTH2 − CD56 − ILC1, CRTH2 + ILC2 and CD117 + CRTH2 − <t>NKp44</t> +/− ILC3 (a, upper panel) . For ILC labelling following 4h of stimulation: ILCs were gated as live, CD45 + lymphocytes that were Lin − (CD19, CD11c, CD11b, CD1a, CD14, CD34, CD123, TCRαβ, TCRγδ, BDCA2, CD235A and FcεR1) CD3 − CD161 + , followed by defining subsets as IFN‐γ + IL‐17 − CD56 − ILC1, IFN‐γ − IL‐17 − IL‐13 + ILC2 and IFN‐γ − IL‐17 + ILC3 (a, lower panel) . Gates were set based on FMO controls (see Supplementary figures 1 and 2 ). Percentage of CD161 + CD127 + ILC of CD45 + live cells (b) and ILC subsets based on surface staining, expressed as % of CD161 + CD127 + ILC (c) . Bar graphs on (b) , (c) , (e) and (f) represent the median values per group; error bars represent the interquartile range (IQR). Spearman’s correlation between % of ILC1 and FEV1/FVC%, %DL CO , total CAT score and score on CAT question 4 (grade of breathlessness when walking up hill or a flight of stairs) (d) . Percentages of ILC subsets based on cytokine staining, expressed as the % of CD161 + ILC (e) . Percentage of Lin + CD56 − CD3 + lymphocyte subsets based on cytokine staining, expressed as the % of CD45 + , live cells: IFN‐γ + IL‐17 − T, IFN‐γ − IL‐17 − IL‐13 + T and IFN‐γ − IL‐17 + T (f) . Spearman’s correlation between % of ILC1 and % of IFN‐γ + ILC (g) and % of IL‐13 + ILC and FEV1% predicted (h) in control subjects (clear dots) and COPD patients (black dots). Group sizes: GOLD I = 3 or 4; GOLD II = 8 or 9; GOLD III = 2, never‐smoking controls = 3, ex‐smoking controls = 3, smoking controls = 3.
    Allophycocyanin Apc Conjugated Anti Nkp44 P44 8, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allophycocyanin apc conjugated anti nkp44 p44 8/product/BioLegend
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allophycocyanin apc conjugated anti nkp44 p44 8 - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

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    Quantification of pulmonary ILC in COPD patients and correlation with clinical parameters. For flow cytometric analysis, ILCs were surface‐stained and gated as live, CD45 + lymphocytes that are Lin − (CD3, CD19, CD11c, CD11b, CD1a, CD14, CD34, CD123, TCRαβ, TCRγδ, BDCA2, CD235A and FcεR1) CD161 + CD127 + . ILC subsets were further defined as CD117 − CRTH2 − CD56 − ILC1, CRTH2 + ILC2 and CD117 + CRTH2 − NKp44 +/− ILC3 (a, upper panel) . For ILC labelling following 4h of stimulation: ILCs were gated as live, CD45 + lymphocytes that were Lin − (CD19, CD11c, CD11b, CD1a, CD14, CD34, CD123, TCRαβ, TCRγδ, BDCA2, CD235A and FcεR1) CD3 − CD161 + , followed by defining subsets as IFN‐γ + IL‐17 − CD56 − ILC1, IFN‐γ − IL‐17 − IL‐13 + ILC2 and IFN‐γ − IL‐17 + ILC3 (a, lower panel) . Gates were set based on FMO controls (see Supplementary figures 1 and 2 ). Percentage of CD161 + CD127 + ILC of CD45 + live cells (b) and ILC subsets based on surface staining, expressed as % of CD161 + CD127 + ILC (c) . Bar graphs on (b) , (c) , (e) and (f) represent the median values per group; error bars represent the interquartile range (IQR). Spearman’s correlation between % of ILC1 and FEV1/FVC%, %DL CO , total CAT score and score on CAT question 4 (grade of breathlessness when walking up hill or a flight of stairs) (d) . Percentages of ILC subsets based on cytokine staining, expressed as the % of CD161 + ILC (e) . Percentage of Lin + CD56 − CD3 + lymphocyte subsets based on cytokine staining, expressed as the % of CD45 + , live cells: IFN‐γ + IL‐17 − T, IFN‐γ − IL‐17 − IL‐13 + T and IFN‐γ − IL‐17 + T (f) . Spearman’s correlation between % of ILC1 and % of IFN‐γ + ILC (g) and % of IL‐13 + ILC and FEV1% predicted (h) in control subjects (clear dots) and COPD patients (black dots). Group sizes: GOLD I = 3 or 4; GOLD II = 8 or 9; GOLD III = 2, never‐smoking controls = 3, ex‐smoking controls = 3, smoking controls = 3.

    Journal: Clinical & Translational Immunology

    Article Title: Quantification and role of innate lymphoid cell subsets in Chronic Obstructive Pulmonary Disease

    doi: 10.1002/cti2.1287

    Figure Lengend Snippet: Quantification of pulmonary ILC in COPD patients and correlation with clinical parameters. For flow cytometric analysis, ILCs were surface‐stained and gated as live, CD45 + lymphocytes that are Lin − (CD3, CD19, CD11c, CD11b, CD1a, CD14, CD34, CD123, TCRαβ, TCRγδ, BDCA2, CD235A and FcεR1) CD161 + CD127 + . ILC subsets were further defined as CD117 − CRTH2 − CD56 − ILC1, CRTH2 + ILC2 and CD117 + CRTH2 − NKp44 +/− ILC3 (a, upper panel) . For ILC labelling following 4h of stimulation: ILCs were gated as live, CD45 + lymphocytes that were Lin − (CD19, CD11c, CD11b, CD1a, CD14, CD34, CD123, TCRαβ, TCRγδ, BDCA2, CD235A and FcεR1) CD3 − CD161 + , followed by defining subsets as IFN‐γ + IL‐17 − CD56 − ILC1, IFN‐γ − IL‐17 − IL‐13 + ILC2 and IFN‐γ − IL‐17 + ILC3 (a, lower panel) . Gates were set based on FMO controls (see Supplementary figures 1 and 2 ). Percentage of CD161 + CD127 + ILC of CD45 + live cells (b) and ILC subsets based on surface staining, expressed as % of CD161 + CD127 + ILC (c) . Bar graphs on (b) , (c) , (e) and (f) represent the median values per group; error bars represent the interquartile range (IQR). Spearman’s correlation between % of ILC1 and FEV1/FVC%, %DL CO , total CAT score and score on CAT question 4 (grade of breathlessness when walking up hill or a flight of stairs) (d) . Percentages of ILC subsets based on cytokine staining, expressed as the % of CD161 + ILC (e) . Percentage of Lin + CD56 − CD3 + lymphocyte subsets based on cytokine staining, expressed as the % of CD45 + , live cells: IFN‐γ + IL‐17 − T, IFN‐γ − IL‐17 − IL‐13 + T and IFN‐γ − IL‐17 + T (f) . Spearman’s correlation between % of ILC1 and % of IFN‐γ + ILC (g) and % of IL‐13 + ILC and FEV1% predicted (h) in control subjects (clear dots) and COPD patients (black dots). Group sizes: GOLD I = 3 or 4; GOLD II = 8 or 9; GOLD III = 2, never‐smoking controls = 3, ex‐smoking controls = 3, smoking controls = 3.

    Article Snippet: To distinguish ILC subsets based on surface staining, the following antibodies were used: fluorescein isothiocyanate (FITC)‐conjugated anti‐CD45 (HI30), allophycocyanin (APC)‐conjugated anti‐NKp44 (P44‐8), phycoerythrin (PE)/indotricarbocya nine (Cy7)‐conjugated anti‐CD117 (104D2), Brilliant Violet 421TM‐conjugated anti‐CD127 (A019D5), Brilliant Violet 605TM‐conjugated anti‐CD56 (HCD56) (all from BioLegend, San Diego, CA), PE‐conjugated CD161 (HP3610, eBioscience) and biotinylated anti‐CRTH2 (BM19; Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with streptavidin‐APC‐Cy7 (BD Biosciences, San Jose, CA).

    Techniques: Staining