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Becton Dickinson mouse anti human cd13
Mouse Anti Human Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti human cd13/product/Becton Dickinson
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mouse anti human cd13 - by Bioz Stars, 2024-05
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Becton Dickinson cd13
hA-MSC markers. Graph showing expression of positive (CD90, CD29, <t>CD13,</t> and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).
Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd13/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cd13 - by Bioz Stars, 2024-05
86/100 stars

Images

1) Product Images from "Human Amniotic MSC Response in LPS-Stimulated Ascites from Patients with Cirrhosis: FOXO1 Gene and Th17 Activation in Enhanced Antibacterial Activation"

Article Title: Human Amniotic MSC Response in LPS-Stimulated Ascites from Patients with Cirrhosis: FOXO1 Gene and Th17 Activation in Enhanced Antibacterial Activation

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms25052801

hA-MSC markers. Graph showing expression of positive (CD90, CD29, CD13, and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).
Figure Legend Snippet: hA-MSC markers. Graph showing expression of positive (CD90, CD29, CD13, and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).

Techniques Used: Expressing, Isolation, Flow Cytometry, Staining


Structured Review

Becton Dickinson cd13
Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd13/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cd13 - by Bioz Stars, 2024-05
86/100 stars

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Becton Dickinson cd13
Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd13/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cd13 - by Bioz Stars, 2024-05
86/100 stars

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Becton Dickinson cd13 alexa fluor 647
Cd13 Alexa Fluor 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd13 alexa fluor 647/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
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cd13 alexa fluor 647 - by Bioz Stars, 2024-05
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Becton Dickinson cd13 cd33
Cd13 Cd33, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd13 cd33 - by Bioz Stars, 2024-05
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Becton Dickinson cd13
Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd13/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cd13 - by Bioz Stars, 2024-05
86/100 stars

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Becton Dickinson cd13 alexa fluor 647
<t>CD13</t> + cells increase in mouse and human brains at a delayed time point after ischemic stroke. A A decrease in brain CD13 + cell numbers acutely after MCAO and MFI of CD13 after stroke ( n = 3–5/gp). B Increase in the brain CD13 + lectin + cells at day 15 post-MCAO ( n = 4/gp). C An increase in CD13 + percentage area at a subacute time point after stroke in human AIS patients. Magnification 20X, scale bar 50 µm, ( n = 8–9). Data presented as mean ± SEM. Two-group comparisons were analyzed by unpaired t-test with Welch’s correction. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test (** p < 0.01, *** p < 0.001, **** p < 0.0001)
Cd13 Alexa Fluor 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd13 alexa fluor 647/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cd13 alexa fluor 647 - by Bioz Stars, 2024-05
86/100 stars

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1) Product Images from "CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery"

Article Title: CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery

Journal: Journal of Neuroinflammation

doi: 10.1186/s12974-023-02918-3

CD13 + cells increase in mouse and human brains at a delayed time point after ischemic stroke. A A decrease in brain CD13 + cell numbers acutely after MCAO and MFI of CD13 after stroke ( n = 3–5/gp). B Increase in the brain CD13 + lectin + cells at day 15 post-MCAO ( n = 4/gp). C An increase in CD13 + percentage area at a subacute time point after stroke in human AIS patients. Magnification 20X, scale bar 50 µm, ( n = 8–9). Data presented as mean ± SEM. Two-group comparisons were analyzed by unpaired t-test with Welch’s correction. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test (** p < 0.01, *** p < 0.001, **** p < 0.0001)
Figure Legend Snippet: CD13 + cells increase in mouse and human brains at a delayed time point after ischemic stroke. A A decrease in brain CD13 + cell numbers acutely after MCAO and MFI of CD13 after stroke ( n = 3–5/gp). B Increase in the brain CD13 + lectin + cells at day 15 post-MCAO ( n = 4/gp). C An increase in CD13 + percentage area at a subacute time point after stroke in human AIS patients. Magnification 20X, scale bar 50 µm, ( n = 8–9). Data presented as mean ± SEM. Two-group comparisons were analyzed by unpaired t-test with Welch’s correction. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Techniques Used:

CD13 impedes acute stroke recovery and promotes neutrophil trans-migration. A No gross anatomical difference in large blood vessels between wild-type and CD13KO naïve mice ( n = 3). B Smaller infarcts ( n = 5–6/group) and C lower NDS ( n = 10–11/group) in CD13KO compared to WT after MCAO. D No difference in cerebral blood flow between CD13 wild type and CD13 knockout ( n = 3). E Higher microglia counts in the ipsilateral hemisphere of CD13KO mice after stroke. F No difference in the infiltrated monocytes in the ipsilateral hemisphere of CD13KO versus WT mice. G A decrease in ipsilateral infiltrated neutrophils in the CD13KO mice compared to the WT. n = 5–6/gp. Data presented as mean ± SEM. Two-group comparisons were analyzed by unpaired t-test with Welch’s correction. NDS analyzed by Mann–Whitney test and presented as a median with an interquartile range. Two-way ANOVA with Tukey’s multiple comparisons test was used to analyze flow cytometry data (* p < 0.05, ** p < 0.01, *** p < 0.001)
Figure Legend Snippet: CD13 impedes acute stroke recovery and promotes neutrophil trans-migration. A No gross anatomical difference in large blood vessels between wild-type and CD13KO naïve mice ( n = 3). B Smaller infarcts ( n = 5–6/group) and C lower NDS ( n = 10–11/group) in CD13KO compared to WT after MCAO. D No difference in cerebral blood flow between CD13 wild type and CD13 knockout ( n = 3). E Higher microglia counts in the ipsilateral hemisphere of CD13KO mice after stroke. F No difference in the infiltrated monocytes in the ipsilateral hemisphere of CD13KO versus WT mice. G A decrease in ipsilateral infiltrated neutrophils in the CD13KO mice compared to the WT. n = 5–6/gp. Data presented as mean ± SEM. Two-group comparisons were analyzed by unpaired t-test with Welch’s correction. NDS analyzed by Mann–Whitney test and presented as a median with an interquartile range. Two-way ANOVA with Tukey’s multiple comparisons test was used to analyze flow cytometry data (* p < 0.05, ** p < 0.01, *** p < 0.001)

Techniques Used: Migration, Knock-Out, MANN-WHITNEY, Flow Cytometry

CD13 plays a critical role in post-stroke cognitive recovery at chronic time points. A percentage alterations of Y-maze, escape latency, and the number of incorrect entries on Barnes maze, distance moved on an open field at day 7 post-MCAO. B percentage alterations of Y-maze, escape latency and the number of incorrect entries on Barnes maze, distance moved on an open field at day 28 post-MCAO. Data presented as mean ± SEM. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. n = 6–13/gp. (* p < 0.05, ** p < 0.01)
Figure Legend Snippet: CD13 plays a critical role in post-stroke cognitive recovery at chronic time points. A percentage alterations of Y-maze, escape latency, and the number of incorrect entries on Barnes maze, distance moved on an open field at day 7 post-MCAO. B percentage alterations of Y-maze, escape latency and the number of incorrect entries on Barnes maze, distance moved on an open field at day 28 post-MCAO. Data presented as mean ± SEM. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. n = 6–13/gp. (* p < 0.05, ** p < 0.01)

Techniques Used:

CD13 KO mice had increased tissue loss, inflammation, and white matter injury at day 30 post-MCAO. A Ventricle area. B percentage tissue loss at day 30 post-MCAO. C Neuron counts day 30 post-MCAO. D Iba-1 and E GFAP counts in the peri-infarct area. F MBP intensity in CC and G striatum. H increased Iba-1 and I GFAP counts in CC at day 30 post-MCAO. Magnification 20X. Scale bar 50 µm. Data presented as mean ± SEM. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. n = 6/gp. (* p < 0.05, ** p < 0.01, *** p < 0.001)
Figure Legend Snippet: CD13 KO mice had increased tissue loss, inflammation, and white matter injury at day 30 post-MCAO. A Ventricle area. B percentage tissue loss at day 30 post-MCAO. C Neuron counts day 30 post-MCAO. D Iba-1 and E GFAP counts in the peri-infarct area. F MBP intensity in CC and G striatum. H increased Iba-1 and I GFAP counts in CC at day 30 post-MCAO. Magnification 20X. Scale bar 50 µm. Data presented as mean ± SEM. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. n = 6/gp. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Techniques Used:

CD13 is involved in post-stroke angiogenesis. A Percentage lectin + area and pericyte coverage in the peri-infarct area. B Avg. vessel length and the total number of endpoints at day 30 post-MCAO. Magnification 20×. Scale bar 50 µm. C BrdU + lectin + counts. Magnification 40×. Scale bar 20 µm. Data presented as mean ± SEM. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. n = 6/gp. (* p < 0.05, ** p < 0.01)
Figure Legend Snippet: CD13 is involved in post-stroke angiogenesis. A Percentage lectin + area and pericyte coverage in the peri-infarct area. B Avg. vessel length and the total number of endpoints at day 30 post-MCAO. Magnification 20×. Scale bar 50 µm. C BrdU + lectin + counts. Magnification 40×. Scale bar 20 µm. Data presented as mean ± SEM. Three-group data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. n = 6/gp. (* p < 0.05, ** p < 0.01)

Techniques Used:


Structured Review

Becton Dickinson mouse anti p150
Mouse Anti P150, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti p150/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
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mouse anti p150 - by Bioz Stars, 2024-05
86/100 stars

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Becton Dickinson mouse anti p150
a Representative spinning-disk confocal time-series of control and Spindly-depleted U2OS cells expressing GFP-CENP-E T422A. Time scale: hour:min. Scale bar: 10 μm. b Immunoblot of Spindly depletion in GFP-CENP-E T422A cells. Four independent experiments showed similar results. c GFP-CENP-E T422A intensity at KTs at different time points after NEB in CENPA-mCherry-expressing cells. Dots and dashed lines represent mean values and SD, respectively. The solid thick line represents the fitted curve. N (number of cells, number of independent experiments): T422A + siCTRL (10, 4), T422A + siSPDLY (10, 2). d Immunofluorescence-based quantification of GFP-CENP-E intensities at the spindle poles in metaphase/pseudo-metaphase. Violin plots with median (thick dashed lines) and quartiles (light dashed lines) are presented. N (number of spindle poles, number of independent experiments) WT (66, 3) T422A (84, 3), WT + siSPDLY (90, 3), T422A + siSPDLY (79, 3). Statistical significance was determined by the Mann–Whitney U -test (unpaired, two-tailed; no normal distribution). p values are indicated. e Percentage of pseudo-metaphases with GFP-CENP-E T422A accumulated at the pole, represented with mean and SEM. N (number of cells, number of independent experiments): siCTRL (150, 3), siSPDLY (150, 3), siSPDLY + SPDLY WT (32, 3) and siSPDLY + SPDLY SB mutant (30, 3) in 3 immunostainings. Scale bar: 10 μm. f Co-immunoprecipitation of GFP-CEN-PE WT with two subunits of the dynein complex <t>dynactin/p150</t> and dynein light intermediate chain/LIC1 from mitotic enriched HEK293 cells. Three independent experiments showed similar results.
Mouse Anti P150, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti p150/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti p150 - by Bioz Stars, 2024-05
86/100 stars

Images

1) Product Images from "CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly"

Article Title: CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly

Journal: Nature Communications

doi: 10.1038/s41467-023-41091-2

a Representative spinning-disk confocal time-series of control and Spindly-depleted U2OS cells expressing GFP-CENP-E T422A. Time scale: hour:min. Scale bar: 10 μm. b Immunoblot of Spindly depletion in GFP-CENP-E T422A cells. Four independent experiments showed similar results. c GFP-CENP-E T422A intensity at KTs at different time points after NEB in CENPA-mCherry-expressing cells. Dots and dashed lines represent mean values and SD, respectively. The solid thick line represents the fitted curve. N (number of cells, number of independent experiments): T422A + siCTRL (10, 4), T422A + siSPDLY (10, 2). d Immunofluorescence-based quantification of GFP-CENP-E intensities at the spindle poles in metaphase/pseudo-metaphase. Violin plots with median (thick dashed lines) and quartiles (light dashed lines) are presented. N (number of spindle poles, number of independent experiments) WT (66, 3) T422A (84, 3), WT + siSPDLY (90, 3), T422A + siSPDLY (79, 3). Statistical significance was determined by the Mann–Whitney U -test (unpaired, two-tailed; no normal distribution). p values are indicated. e Percentage of pseudo-metaphases with GFP-CENP-E T422A accumulated at the pole, represented with mean and SEM. N (number of cells, number of independent experiments): siCTRL (150, 3), siSPDLY (150, 3), siSPDLY + SPDLY WT (32, 3) and siSPDLY + SPDLY SB mutant (30, 3) in 3 immunostainings. Scale bar: 10 μm. f Co-immunoprecipitation of GFP-CEN-PE WT with two subunits of the dynein complex dynactin/p150 and dynein light intermediate chain/LIC1 from mitotic enriched HEK293 cells. Three independent experiments showed similar results.
Figure Legend Snippet: a Representative spinning-disk confocal time-series of control and Spindly-depleted U2OS cells expressing GFP-CENP-E T422A. Time scale: hour:min. Scale bar: 10 μm. b Immunoblot of Spindly depletion in GFP-CENP-E T422A cells. Four independent experiments showed similar results. c GFP-CENP-E T422A intensity at KTs at different time points after NEB in CENPA-mCherry-expressing cells. Dots and dashed lines represent mean values and SD, respectively. The solid thick line represents the fitted curve. N (number of cells, number of independent experiments): T422A + siCTRL (10, 4), T422A + siSPDLY (10, 2). d Immunofluorescence-based quantification of GFP-CENP-E intensities at the spindle poles in metaphase/pseudo-metaphase. Violin plots with median (thick dashed lines) and quartiles (light dashed lines) are presented. N (number of spindle poles, number of independent experiments) WT (66, 3) T422A (84, 3), WT + siSPDLY (90, 3), T422A + siSPDLY (79, 3). Statistical significance was determined by the Mann–Whitney U -test (unpaired, two-tailed; no normal distribution). p values are indicated. e Percentage of pseudo-metaphases with GFP-CENP-E T422A accumulated at the pole, represented with mean and SEM. N (number of cells, number of independent experiments): siCTRL (150, 3), siSPDLY (150, 3), siSPDLY + SPDLY WT (32, 3) and siSPDLY + SPDLY SB mutant (30, 3) in 3 immunostainings. Scale bar: 10 μm. f Co-immunoprecipitation of GFP-CEN-PE WT with two subunits of the dynein complex dynactin/p150 and dynein light intermediate chain/LIC1 from mitotic enriched HEK293 cells. Three independent experiments showed similar results.

Techniques Used: Expressing, Western Blot, Immunofluorescence, MANN-WHITNEY, Two Tailed Test, Mutagenesis, Immunoprecipitation

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    Becton Dickinson mouse anti human cd13
    Mouse Anti Human Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson cd13
    hA-MSC markers. Graph showing expression of positive (CD90, CD29, <t>CD13,</t> and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).
    Cd13, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd13/product/Becton Dickinson
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    Becton Dickinson cd13 alexa fluor 647
    hA-MSC markers. Graph showing expression of positive (CD90, CD29, <t>CD13,</t> and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).
    Cd13 Alexa Fluor 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd13 alexa fluor 647/product/Becton Dickinson
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    Becton Dickinson cd13 cd33
    hA-MSC markers. Graph showing expression of positive (CD90, CD29, <t>CD13,</t> and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).
    Cd13 Cd33, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd13 cd33/product/Becton Dickinson
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    Becton Dickinson mouse anti p150
    hA-MSC markers. Graph showing expression of positive (CD90, CD29, <t>CD13,</t> and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).
    Mouse Anti P150, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti p150/product/Becton Dickinson
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    hA-MSC markers. Graph showing expression of positive (CD90, CD29, CD13, and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).

    Journal: International Journal of Molecular Sciences

    Article Title: Human Amniotic MSC Response in LPS-Stimulated Ascites from Patients with Cirrhosis: FOXO1 Gene and Th17 Activation in Enhanced Antibacterial Activation

    doi: 10.3390/ijms25052801

    Figure Lengend Snippet: hA-MSC markers. Graph showing expression of positive (CD90, CD29, CD13, and CD73) and negative (CD34, CD3, HLA-DR, and CD45) cell surface markers after human amnion mesenchymal stem cell (hA-MSCs) isolation, with representative images of flow cytometry analysis for the quantification of both positive and negative surface markers (Blue isotype control, green positive staining; the panel is representative of three different analyses).

    Article Snippet: The expression of hA-MSC markers was analyzed by procuring the isolated cells, washing them twice with FACS buffer (PBS containing 0.3% bovine serum albumin and 0.1% sodium azide), and staining them with antibodies against positive cell surface antigens, CD90 (PE, Clone 5E10, Mouse BALB/c IgG1, κ, BD Biosciences, San Jose, CA, USA), CD73 (APC, Clone AD2, Mouse IgG1, k, Miltenyi Biotec), CD29 (APC, Clone MAR4, Mouse BALB/c IgG1κ BD), and CD13 (APC, Clone WM15, Mouse IgG1 κ BD), and negative cell surface antigens CD45 (PE-Cy7, Clone HI30, Mouse IgG1 κ BD), CD34 (PE-Cy7, Clone 8G12, Mouse BALB/c IgG1 κ BD), CD3 (PerCP-Cy5.5, Clone UCHT1, BD), and HLA-DR (PE, Clone G46-6, BD), for the characterization of MSCs.

    Techniques: Expressing, Isolation, Flow Cytometry, Staining