anti cd11b primary antibody  (Abcam)

 
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    Name:
    Anti CD11b antibody EPR1344
    Description:

    Catalog Number:
    AB133357
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    Structured Review

    Abcam anti cd11b primary antibody
    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 <t>(CD11b)</t> (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    https://www.bioz.com/result/anti cd11b primary antibody/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd11b primary antibody - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling"

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04831-3

    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)
    Figure Legend Snippet: Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Techniques Used: Mouse Assay

    2) Product Images from "miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling"

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04831-3

    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)
    Figure Legend Snippet: Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Techniques Used: Mouse Assay

    Related Articles

    Incubation:

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling
    Article Snippet: Alternatively, freshly cut tissues were analyzed for CD11b expression and immunohistochemistry was performed using the Ventana DiscoveryXT platform. .. In brief, tissue sections were incubated in Tris EDTA buffer at 95 °C to retrieve antigenicity, followed by incubation with the anti-CD11b primary antibody (ab133357, Abcam). .. Bound antibodies were incubated with secondary horse radish peroxidase-conjugated antibodies which was followed by Chromomap DAB detection.

    Article Title: IL-11 induces differentiation of myeloid-derived suppressor cells through activation of STAT3 signalling pathway
    Article Snippet: .. Endogenous peroxidase activity was blocked with 0.3% H2 O2 at room temperature for 10 min. After protein blocking at room temperature for 10 min, slides were incubated with a polyclonal rabbit anti-human IL-11 (RPA057Hu01, Uscn Life Science, USA), a polyclonal rabbit anti-human phospho-STAT3 (Tyr705) antibody (9145, Cell Signaling Technology), a monoclonal mouse anit-CD14 antibody (MY4), or a rabbit monoclonal anti-CD11b antibody (EPR1344, Abcam, Cambridge, UK) overnight at 4 °C. .. Sections were then incubated at room temperature for 15 min with a horseradish peroxidase-labelled anti-rabbit Igs.

    Article Title: Endothelial Glycocalyx Disorders May Be Associated With Extended Inflammation During Endotoxemia in a Diabetic Mouse Model
    Article Snippet: .. Immunohistochemistry Lung sections were incubated with primary antibodies against the neutrophil and macrophage surface marker CD11b (ab133357; Abcam, Cambridge, United Kingdom), the vascular endothelial cell marker CD31 (DIO-310; Dianova GmbH, Hamburg, Germany), and the activated macrophage surface marker Iba-1 (019-19741; Wako Pure Chemical, Osaka, Japan). .. Sections were immunostained with the Vectastain Elite ABC system (Vector Laboratories, Burlingame, CA, United States) as previously described ( ).

    Activity Assay:

    Article Title: IL-11 induces differentiation of myeloid-derived suppressor cells through activation of STAT3 signalling pathway
    Article Snippet: .. Endogenous peroxidase activity was blocked with 0.3% H2 O2 at room temperature for 10 min. After protein blocking at room temperature for 10 min, slides were incubated with a polyclonal rabbit anti-human IL-11 (RPA057Hu01, Uscn Life Science, USA), a polyclonal rabbit anti-human phospho-STAT3 (Tyr705) antibody (9145, Cell Signaling Technology), a monoclonal mouse anit-CD14 antibody (MY4), or a rabbit monoclonal anti-CD11b antibody (EPR1344, Abcam, Cambridge, UK) overnight at 4 °C. .. Sections were then incubated at room temperature for 15 min with a horseradish peroxidase-labelled anti-rabbit Igs.

    Blocking Assay:

    Article Title: IL-11 induces differentiation of myeloid-derived suppressor cells through activation of STAT3 signalling pathway
    Article Snippet: .. Endogenous peroxidase activity was blocked with 0.3% H2 O2 at room temperature for 10 min. After protein blocking at room temperature for 10 min, slides were incubated with a polyclonal rabbit anti-human IL-11 (RPA057Hu01, Uscn Life Science, USA), a polyclonal rabbit anti-human phospho-STAT3 (Tyr705) antibody (9145, Cell Signaling Technology), a monoclonal mouse anit-CD14 antibody (MY4), or a rabbit monoclonal anti-CD11b antibody (EPR1344, Abcam, Cambridge, UK) overnight at 4 °C. .. Sections were then incubated at room temperature for 15 min with a horseradish peroxidase-labelled anti-rabbit Igs.

    Immunohistochemistry:

    Article Title: Endothelial Glycocalyx Disorders May Be Associated With Extended Inflammation During Endotoxemia in a Diabetic Mouse Model
    Article Snippet: .. Immunohistochemistry Lung sections were incubated with primary antibodies against the neutrophil and macrophage surface marker CD11b (ab133357; Abcam, Cambridge, United Kingdom), the vascular endothelial cell marker CD31 (DIO-310; Dianova GmbH, Hamburg, Germany), and the activated macrophage surface marker Iba-1 (019-19741; Wako Pure Chemical, Osaka, Japan). .. Sections were immunostained with the Vectastain Elite ABC system (Vector Laboratories, Burlingame, CA, United States) as previously described ( ).

    Article Title: A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studies
    Article Snippet: Image acquisitionPictures were captured with Zeiss Axio-Scan.Z1 using ZEN software (Zeiss) or with the MEA53100 Eclipse Ti-E inverted microscope (Nikon) using MQS31000 NIS-ELEMENTS AR software (Nikon) with camera MQA11550 DS-Qi1MC for bright field images and MQA11010 DS-Fi1 for immunofluorescent images. .. Immunohistochemistry and immunofluorescence staining Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186, Abcam), rabbit anti CD11b (ab133357, Abcam), rabbit anti Iba1 (019-1974, Wako), chicken anti GFP (ab1397, Abcam), mouse anti Bcat1 (TA504360, OriGene), mouse anti-GFAP (644701, BioLegend). .. Secondary antibodies were: goat anti-rabbit Alexa Fluor 546 (A11071 Thermo Fischer Scientific) and goat anti-mouse Alexa Fluor 555 (A21422, Thermo Fischer Scientific).

    Marker:

    Article Title: Endothelial Glycocalyx Disorders May Be Associated With Extended Inflammation During Endotoxemia in a Diabetic Mouse Model
    Article Snippet: .. Immunohistochemistry Lung sections were incubated with primary antibodies against the neutrophil and macrophage surface marker CD11b (ab133357; Abcam, Cambridge, United Kingdom), the vascular endothelial cell marker CD31 (DIO-310; Dianova GmbH, Hamburg, Germany), and the activated macrophage surface marker Iba-1 (019-19741; Wako Pure Chemical, Osaka, Japan). .. Sections were immunostained with the Vectastain Elite ABC system (Vector Laboratories, Burlingame, CA, United States) as previously described ( ).

    Staining:

    Article Title: Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells
    Article Snippet: Sections were stained with the primary antibodies to the following: FXII (1:50, Proteintech), human CD11c (1:100, clone 3.9, Abcam, Cambridge, UK), human albumin (1:50, Origene Technologies, Rockville, MD, USA), ionized calcium-binding adapter molecule 1 (1:250, polyclonal, Wako Chemicals GmbH, Neuss, Germany), glial fibrillary acidic protein (GFAP; 1:1,000, clone G-A-5, Sigma-Aldrich) and mouse CD11c (1:1,000, clone N418, eBioscience, San Diego, CA, USA). .. Astrocyte and microglia cultures were stained with anti-GFAP and anti-CD11b (1:1,000, clone EPR1344, Abcam), respectively. .. In addition, both were stained with anti-MHC-I (1:200, clone ER-HR52, Abcam) and anti-MHC-II (1:50, clone NIMR-4, Abcam).

    Article Title: A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studies
    Article Snippet: Image acquisitionPictures were captured with Zeiss Axio-Scan.Z1 using ZEN software (Zeiss) or with the MEA53100 Eclipse Ti-E inverted microscope (Nikon) using MQS31000 NIS-ELEMENTS AR software (Nikon) with camera MQA11550 DS-Qi1MC for bright field images and MQA11010 DS-Fi1 for immunofluorescent images. .. Immunohistochemistry and immunofluorescence staining Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186, Abcam), rabbit anti CD11b (ab133357, Abcam), rabbit anti Iba1 (019-1974, Wako), chicken anti GFP (ab1397, Abcam), mouse anti Bcat1 (TA504360, OriGene), mouse anti-GFAP (644701, BioLegend). .. Secondary antibodies were: goat anti-rabbit Alexa Fluor 546 (A11071 Thermo Fischer Scientific) and goat anti-mouse Alexa Fluor 555 (A21422, Thermo Fischer Scientific).

    Immunofluorescence:

    Article Title: A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studies
    Article Snippet: Image acquisitionPictures were captured with Zeiss Axio-Scan.Z1 using ZEN software (Zeiss) or with the MEA53100 Eclipse Ti-E inverted microscope (Nikon) using MQS31000 NIS-ELEMENTS AR software (Nikon) with camera MQA11550 DS-Qi1MC for bright field images and MQA11010 DS-Fi1 for immunofluorescent images. .. Immunohistochemistry and immunofluorescence staining Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186, Abcam), rabbit anti CD11b (ab133357, Abcam), rabbit anti Iba1 (019-1974, Wako), chicken anti GFP (ab1397, Abcam), mouse anti Bcat1 (TA504360, OriGene), mouse anti-GFAP (644701, BioLegend). .. Secondary antibodies were: goat anti-rabbit Alexa Fluor 546 (A11071 Thermo Fischer Scientific) and goat anti-mouse Alexa Fluor 555 (A21422, Thermo Fischer Scientific).

    Formalin-fixed Paraffin-Embedded:

    Article Title: A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studies
    Article Snippet: Image acquisitionPictures were captured with Zeiss Axio-Scan.Z1 using ZEN software (Zeiss) or with the MEA53100 Eclipse Ti-E inverted microscope (Nikon) using MQS31000 NIS-ELEMENTS AR software (Nikon) with camera MQA11550 DS-Qi1MC for bright field images and MQA11010 DS-Fi1 for immunofluorescent images. .. Immunohistochemistry and immunofluorescence staining Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186, Abcam), rabbit anti CD11b (ab133357, Abcam), rabbit anti Iba1 (019-1974, Wako), chicken anti GFP (ab1397, Abcam), mouse anti Bcat1 (TA504360, OriGene), mouse anti-GFAP (644701, BioLegend). .. Secondary antibodies were: goat anti-rabbit Alexa Fluor 546 (A11071 Thermo Fischer Scientific) and goat anti-mouse Alexa Fluor 555 (A21422, Thermo Fischer Scientific).

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  • 93
    Abcam anti cd11b antibody
    DUSP12 deficiency aggravates HFHC‐induced liver fibrosis and inflammation. (A) Body weight and (B) liver weight in DUSP12‐Flox or DUSP12‐CKO mice fed an HFHC for 16 weeks (n = 10/group). (C) Representative H E staining of liver sections obtained from Flox or DUSP12‐CKO mice after 16 weeks of HFHC feeding (n = 6/group). Scale bar, 100 μm. The NAFLD activity score of Flox or DUSP12‐CKO mice after 16 weeks of HFHC feeding (n = 6/group). (D) Triglyceride and (E) TC levels in Flox or DUSP12‐CKO mice fed an HFHC for 16 weeks (n = 10/group). (F) Relative mRNA levels of lipogenesis‐related genes SCD1, CD36, PPARγ, and PPARα were tested by real‐time quantitative PCR (n = 4/group). (G) Fasting blood glucose levels were measured in DUSP12‐Flox or DUSP12‐CKO mice fed an HFHC every 4 weeks from 0 to 16 weeks (n = 10 in each group). (H,I) GTT (H) and ITT (I) were performed on DUSP12‐Flox and DUSP12‐CKO mice after HFHC feeding for 14 weeks and 15 weeks, respectively (for each test, n = 10/group). (J) Representative images showing immunofluorescence staining for <t>CD11b</t> in the livers of the indicated mice fed an HFHC for 16 weeks. Nuclei were labeled with 4′,6‐diamidino‐2‐phenylindole (blue) (n = 4 mice/group). Scale bar, 25 µm. (K) Relative mRNA levels of cytokines in DUSP12‐Flox and DUSP12‐CKO mice after HFHC feeding (n = 4 mice/group). (L) Representative picrosirius red staining of liver sections from DUSP12‐Flox and DUSP12‐CKO mice after HFHC diet for 16 weeks (n = 6 mice/group). (M) mRNA levels of profibrotic genes in livers from mice in the indicated groups (n = 4 mice/group). mRNA expression of target genes was normalized to that of β‐actin. All data represent the mean ± SEM. * P
    Anti Cd11b Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd11b antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd11b antibody - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    97
    Abcam anti cd11b antibody epr1344
    Modulation of inflammatory cells under endotoxemic conditions. (A1) <t>CD11b-positive</t> cells were quantified as numbers of cells per high-power field. (A2) Representative images of CD11b cells in immunostained lung specimens. (B1) Iba-1 positive cells were quantified as the number of cells per high-power field. (B2) Representative images of Iba-1 in immunostained lung samples. *, P
    Anti Cd11b Antibody Epr1344, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd11b antibody epr1344/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd11b antibody epr1344 - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    DUSP12 deficiency aggravates HFHC‐induced liver fibrosis and inflammation. (A) Body weight and (B) liver weight in DUSP12‐Flox or DUSP12‐CKO mice fed an HFHC for 16 weeks (n = 10/group). (C) Representative H E staining of liver sections obtained from Flox or DUSP12‐CKO mice after 16 weeks of HFHC feeding (n = 6/group). Scale bar, 100 μm. The NAFLD activity score of Flox or DUSP12‐CKO mice after 16 weeks of HFHC feeding (n = 6/group). (D) Triglyceride and (E) TC levels in Flox or DUSP12‐CKO mice fed an HFHC for 16 weeks (n = 10/group). (F) Relative mRNA levels of lipogenesis‐related genes SCD1, CD36, PPARγ, and PPARα were tested by real‐time quantitative PCR (n = 4/group). (G) Fasting blood glucose levels were measured in DUSP12‐Flox or DUSP12‐CKO mice fed an HFHC every 4 weeks from 0 to 16 weeks (n = 10 in each group). (H,I) GTT (H) and ITT (I) were performed on DUSP12‐Flox and DUSP12‐CKO mice after HFHC feeding for 14 weeks and 15 weeks, respectively (for each test, n = 10/group). (J) Representative images showing immunofluorescence staining for CD11b in the livers of the indicated mice fed an HFHC for 16 weeks. Nuclei were labeled with 4′,6‐diamidino‐2‐phenylindole (blue) (n = 4 mice/group). Scale bar, 25 µm. (K) Relative mRNA levels of cytokines in DUSP12‐Flox and DUSP12‐CKO mice after HFHC feeding (n = 4 mice/group). (L) Representative picrosirius red staining of liver sections from DUSP12‐Flox and DUSP12‐CKO mice after HFHC diet for 16 weeks (n = 6 mice/group). (M) mRNA levels of profibrotic genes in livers from mice in the indicated groups (n = 4 mice/group). mRNA expression of target genes was normalized to that of β‐actin. All data represent the mean ± SEM. * P

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Dual Specificity Phosphatase 12 Regulates Hepatic Lipid Metabolism Through Inhibition of the Lipogenesis and Apoptosis Signal–Regulating Kinase 1 Pathways

    doi: 10.1002/hep.30597

    Figure Lengend Snippet: DUSP12 deficiency aggravates HFHC‐induced liver fibrosis and inflammation. (A) Body weight and (B) liver weight in DUSP12‐Flox or DUSP12‐CKO mice fed an HFHC for 16 weeks (n = 10/group). (C) Representative H E staining of liver sections obtained from Flox or DUSP12‐CKO mice after 16 weeks of HFHC feeding (n = 6/group). Scale bar, 100 μm. The NAFLD activity score of Flox or DUSP12‐CKO mice after 16 weeks of HFHC feeding (n = 6/group). (D) Triglyceride and (E) TC levels in Flox or DUSP12‐CKO mice fed an HFHC for 16 weeks (n = 10/group). (F) Relative mRNA levels of lipogenesis‐related genes SCD1, CD36, PPARγ, and PPARα were tested by real‐time quantitative PCR (n = 4/group). (G) Fasting blood glucose levels were measured in DUSP12‐Flox or DUSP12‐CKO mice fed an HFHC every 4 weeks from 0 to 16 weeks (n = 10 in each group). (H,I) GTT (H) and ITT (I) were performed on DUSP12‐Flox and DUSP12‐CKO mice after HFHC feeding for 14 weeks and 15 weeks, respectively (for each test, n = 10/group). (J) Representative images showing immunofluorescence staining for CD11b in the livers of the indicated mice fed an HFHC for 16 weeks. Nuclei were labeled with 4′,6‐diamidino‐2‐phenylindole (blue) (n = 4 mice/group). Scale bar, 25 µm. (K) Relative mRNA levels of cytokines in DUSP12‐Flox and DUSP12‐CKO mice after HFHC feeding (n = 4 mice/group). (L) Representative picrosirius red staining of liver sections from DUSP12‐Flox and DUSP12‐CKO mice after HFHC diet for 16 weeks (n = 6 mice/group). (M) mRNA levels of profibrotic genes in livers from mice in the indicated groups (n = 4 mice/group). mRNA expression of target genes was normalized to that of β‐actin. All data represent the mean ± SEM. * P

    Article Snippet: Immunofluorescence Staining Paraffin sections were labeled with primary antibodies (ab75476, 1:100; Abcam) overnight, followed by incubation with secondary antibody for 1 hour.

    Techniques: Mouse Assay, Staining, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Labeling, Expressing

    Modulation of inflammatory cells under endotoxemic conditions. (A1) CD11b-positive cells were quantified as numbers of cells per high-power field. (A2) Representative images of CD11b cells in immunostained lung specimens. (B1) Iba-1 positive cells were quantified as the number of cells per high-power field. (B2) Representative images of Iba-1 in immunostained lung samples. *, P

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Endothelial Glycocalyx Disorders May Be Associated With Extended Inflammation During Endotoxemia in a Diabetic Mouse Model

    doi: 10.3389/fcell.2021.623582

    Figure Lengend Snippet: Modulation of inflammatory cells under endotoxemic conditions. (A1) CD11b-positive cells were quantified as numbers of cells per high-power field. (A2) Representative images of CD11b cells in immunostained lung specimens. (B1) Iba-1 positive cells were quantified as the number of cells per high-power field. (B2) Representative images of Iba-1 in immunostained lung samples. *, P

    Article Snippet: Immunohistochemistry Lung sections were incubated with primary antibodies against the neutrophil and macrophage surface marker CD11b (ab133357; Abcam, Cambridge, United Kingdom), the vascular endothelial cell marker CD31 (DIO-310; Dianova GmbH, Hamburg, Germany), and the activated macrophage surface marker Iba-1 (019-19741; Wako Pure Chemical, Osaka, Japan).

    Techniques:

    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Journal: Nature Communications

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling

    doi: 10.1038/s41467-018-04831-3

    Figure Lengend Snippet: Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Article Snippet: In brief, tissue sections were incubated in Tris EDTA buffer at 95 °C to retrieve antigenicity, followed by incubation with the anti-CD11b primary antibody (ab133357, Abcam).

    Techniques: Mouse Assay

    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Journal: Nature Communications

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling

    doi: 10.1038/s41467-018-04831-3

    Figure Lengend Snippet: Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Article Snippet: In brief, tissue sections were incubated in Tris EDTA buffer at 95 °C to retrieve antigenicity, followed by incubation with the anti-CD11b primary antibody (ab133357, Abcam).

    Techniques: Mouse Assay