rabbit anti cav2 3  (Alomone Labs)


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    Alomone Labs rabbit anti cav2 3
    Rabbit Anti Cav2 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cav2 3/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cav2 3 - by Bioz Stars, 2022-12
    93/100 stars

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    Alomone Labs anti cav2 3 cacna1e antibody
    Quantification of KCTDs at the plasma and total membrane in HEK cells co-expressing <t>Cav2.3,</t> auxiliary β3 and α2δ1 subunits. Fold increase of KCTD signal at the plasma membrane (PM) compared to the averaged KCTD signal in total membrane (TM). Quantification of KCTDs at the plasma and total membrane in HEK cells co-expressing <t>Cav2.3,</t> auxiliary β3 and α2δ1 subunits.
    Anti Cav2 3 Cacna1e Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav2 3 cacna1e antibody/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti cav2 3 cacna1e antibody - by Bioz Stars, 2022-12
    93/100 stars
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    Quantification of KCTDs at the plasma and total membrane in HEK cells co-expressing Cav2.3, auxiliary β3 and α2δ1 subunits. Fold increase of KCTD signal at the plasma membrane (PM) compared to the averaged KCTD signal in total membrane (TM). Quantification of KCTDs at the plasma and total membrane in HEK cells co-expressing Cav2.3, auxiliary β3 and α2δ1 subunits.

    Journal: eLife

    Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals

    doi: 10.7554/eLife.68274

    Figure Lengend Snippet: Quantification of KCTDs at the plasma and total membrane in HEK cells co-expressing Cav2.3, auxiliary β3 and α2δ1 subunits. Fold increase of KCTD signal at the plasma membrane (PM) compared to the averaged KCTD signal in total membrane (TM). Quantification of KCTDs at the plasma and total membrane in HEK cells co-expressing Cav2.3, auxiliary β3 and α2δ1 subunits.

    Article Snippet: For co-immunoprecipitation assay, lysates were incubated by rotating for 16 hr at 4°C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel), washed, and eluted as described above with subsequent immunoblotting analysis performed as described above.

    Techniques: Expressing

    Co-localization of Cav2.3 with GBR and KCTDs in the active zone of medial habenula terminals. ( A ) Active zones double labeled for Cav2.3 and either GABA B1 (left), KCTD8 (middle), or KCTD12b (right) in IPN replicas. Top row images are from presynaptic terminals in the rostral IPN; bottom row images are from presynaptic terminals in the lateral IPN. Scale bar: 100 nm. ( B ) Quantification of active zone immunolabeling in the rostral and lateral IPN. With the exception of the absence of KCTD12b in lateral IPN terminals, absolute particle numbers per active zone (left graph) and particle densities (middle graph) are comparable between MHb terminals in the rostral and lateral IPN. Right graph: Over 97% of active zones positive for Cav2.3 labeling also show labeling for one of the other molecules (GABA B1 , KCTD8, or KCTD12b), suggesting co-localization of all presynaptic molecules inside the same active zone. Numbers inside the bars indicate the number of replicas used for each quantification. ( C ) Nearest-neighbor distance (NND) for all presynaptic molecules in MHb terminals inside the rostral and lateral IPN based on the real (black line) and simulated random distribution (blue line). Smaller NND values in real distributions compared to simulation suggest clustering of all presynaptic molecules. p-values calculated via Kolmogorov–Smirnov test. Co-localization of Cav2.3 with GBR and KCTDs in the active zone of medial habenula terminals.

    Journal: eLife

    Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals

    doi: 10.7554/eLife.68274

    Figure Lengend Snippet: Co-localization of Cav2.3 with GBR and KCTDs in the active zone of medial habenula terminals. ( A ) Active zones double labeled for Cav2.3 and either GABA B1 (left), KCTD8 (middle), or KCTD12b (right) in IPN replicas. Top row images are from presynaptic terminals in the rostral IPN; bottom row images are from presynaptic terminals in the lateral IPN. Scale bar: 100 nm. ( B ) Quantification of active zone immunolabeling in the rostral and lateral IPN. With the exception of the absence of KCTD12b in lateral IPN terminals, absolute particle numbers per active zone (left graph) and particle densities (middle graph) are comparable between MHb terminals in the rostral and lateral IPN. Right graph: Over 97% of active zones positive for Cav2.3 labeling also show labeling for one of the other molecules (GABA B1 , KCTD8, or KCTD12b), suggesting co-localization of all presynaptic molecules inside the same active zone. Numbers inside the bars indicate the number of replicas used for each quantification. ( C ) Nearest-neighbor distance (NND) for all presynaptic molecules in MHb terminals inside the rostral and lateral IPN based on the real (black line) and simulated random distribution (blue line). Smaller NND values in real distributions compared to simulation suggest clustering of all presynaptic molecules. p-values calculated via Kolmogorov–Smirnov test. Co-localization of Cav2.3 with GBR and KCTDs in the active zone of medial habenula terminals.

    Article Snippet: For co-immunoprecipitation assay, lysates were incubated by rotating for 16 hr at 4°C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel), washed, and eluted as described above with subsequent immunoblotting analysis performed as described above.

    Techniques: Labeling, Immunolabeling

    Allen Brain Atlas images showing lack of expression of Cav2.1 ( http://mouse.brain-map.org/gene/show/12071 ) and Cav2.2 ( http://mouse.brain-map.org/gene/show/12072 ) mRNA in MHb neurons, whereas Cav2.3 shows strong expression in MHb neurons ( http://mouse.brain-map.org/gene/show/12075 ).

    Journal: eLife

    Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals

    doi: 10.7554/eLife.68274

    Figure Lengend Snippet: Allen Brain Atlas images showing lack of expression of Cav2.1 ( http://mouse.brain-map.org/gene/show/12071 ) and Cav2.2 ( http://mouse.brain-map.org/gene/show/12072 ) mRNA in MHb neurons, whereas Cav2.3 shows strong expression in MHb neurons ( http://mouse.brain-map.org/gene/show/12075 ).

    Article Snippet: For co-immunoprecipitation assay, lysates were incubated by rotating for 16 hr at 4°C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel), washed, and eluted as described above with subsequent immunoblotting analysis performed as described above.

    Techniques: Expressing

    SDS-digested freeze-fracture replica labeling confirms Cav2.3 in the active zone of medial habenula terminals in the IPN. ( A ) Example image of a grid-glued replica containing the whole IPN. White dashed line indicates demarcation of rostral/central and lateral subnuclei. Scale bar: 20 µm. ( B ) Example image of a presynaptic P-face and a postsynaptic E-face of a habenular synapse in the rostral IPN that was double labeled with antibodies against AMPA receptors (10 nm gold) and Cav2.3 (5 nm gold). Scale bar: 100 nm. ( C ) Example image of a similar synaptic profile double labeled with antibodies against AMPA receptors (10 nm gold) and Cav2.3 (5 nm gold) in the rostral IPN of a Cav2.3 KO mouse. Scale bar: 100 nm. ( D ) Left: double labeling of a WT carbon-only replica with antibodies against Cav2.3 (5 nm gold) and a mixture of active zone proteins (2 nm gold), including RIM1/2, CAST, and neurexin. Right: the same image with additional coloring of 2 nm (red) and 5 nm (blue) particles and demarcation of the active zone area based on active zone-marker labeling. Scale bars: 100 nm. ( F ) Left: quantification of Cav2.3 labeling densities in the presynaptic P-face in WT and Cav2.3 KO mice. ***p

    Journal: eLife

    Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals

    doi: 10.7554/eLife.68274

    Figure Lengend Snippet: SDS-digested freeze-fracture replica labeling confirms Cav2.3 in the active zone of medial habenula terminals in the IPN. ( A ) Example image of a grid-glued replica containing the whole IPN. White dashed line indicates demarcation of rostral/central and lateral subnuclei. Scale bar: 20 µm. ( B ) Example image of a presynaptic P-face and a postsynaptic E-face of a habenular synapse in the rostral IPN that was double labeled with antibodies against AMPA receptors (10 nm gold) and Cav2.3 (5 nm gold). Scale bar: 100 nm. ( C ) Example image of a similar synaptic profile double labeled with antibodies against AMPA receptors (10 nm gold) and Cav2.3 (5 nm gold) in the rostral IPN of a Cav2.3 KO mouse. Scale bar: 100 nm. ( D ) Left: double labeling of a WT carbon-only replica with antibodies against Cav2.3 (5 nm gold) and a mixture of active zone proteins (2 nm gold), including RIM1/2, CAST, and neurexin. Right: the same image with additional coloring of 2 nm (red) and 5 nm (blue) particles and demarcation of the active zone area based on active zone-marker labeling. Scale bars: 100 nm. ( F ) Left: quantification of Cav2.3 labeling densities in the presynaptic P-face in WT and Cav2.3 KO mice. ***p

    Article Snippet: For co-immunoprecipitation assay, lysates were incubated by rotating for 16 hr at 4°C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel), washed, and eluted as described above with subsequent immunoblotting analysis performed as described above.

    Techniques: Labeling, Marker, Mouse Assay

    Comparison of distribution of silver-enhanced immunogold particles outside the active zone for Cav2.3, KCTD8, KCTD12, and KCTD12b in MHb terminals inside the rostral and lateral IPN. Distribution of particles in samples from two mice was not significantly different. p-values above graph resulted from Kolmogorov–Smirnov test. M1 = mouse 1, M2 = mouse 2, n indicates the total number of particles analyzed. Comparison of distribution of silver-enhanced immunogold particles outside the active zone for Cav2.3, KCTD8, KCTD12, and KCTD12b in MHb terminals inside the rostral and lateral IPN.

    Journal: eLife

    Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals

    doi: 10.7554/eLife.68274

    Figure Lengend Snippet: Comparison of distribution of silver-enhanced immunogold particles outside the active zone for Cav2.3, KCTD8, KCTD12, and KCTD12b in MHb terminals inside the rostral and lateral IPN. Distribution of particles in samples from two mice was not significantly different. p-values above graph resulted from Kolmogorov–Smirnov test. M1 = mouse 1, M2 = mouse 2, n indicates the total number of particles analyzed. Comparison of distribution of silver-enhanced immunogold particles outside the active zone for Cav2.3, KCTD8, KCTD12, and KCTD12b in MHb terminals inside the rostral and lateral IPN.

    Article Snippet: For co-immunoprecipitation assay, lysates were incubated by rotating for 16 hr at 4°C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel), washed, and eluted as described above with subsequent immunoblotting analysis performed as described above.

    Techniques: Mouse Assay

    Absence of KCTD12b leads to a compensatory increase of KCTD8 inside the active zone of ventral MHb terminals. ( A ) Example images of active zones containing Cav2.3 and either GABA B1 (left) or KCTD8 (right) in replicas of WT (upper row) and KCTD12b KO IPN tissue (lower row). Scale bars: 100 nm. ( B ) Quantification of relative densities for Cav2.3, KCTD8, and GABA B1 in active zones located in the rostral IPN of WT and KCTD12b KO mice. Densities were normalized to the average density in MHb terminals inside the lateral IPN of the same replica. The number inside the bars indicates the number of replicas used for quantification. **p

    Journal: eLife

    Article Title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals

    doi: 10.7554/eLife.68274

    Figure Lengend Snippet: Absence of KCTD12b leads to a compensatory increase of KCTD8 inside the active zone of ventral MHb terminals. ( A ) Example images of active zones containing Cav2.3 and either GABA B1 (left) or KCTD8 (right) in replicas of WT (upper row) and KCTD12b KO IPN tissue (lower row). Scale bars: 100 nm. ( B ) Quantification of relative densities for Cav2.3, KCTD8, and GABA B1 in active zones located in the rostral IPN of WT and KCTD12b KO mice. Densities were normalized to the average density in MHb terminals inside the lateral IPN of the same replica. The number inside the bars indicates the number of replicas used for quantification. **p

    Article Snippet: For co-immunoprecipitation assay, lysates were incubated by rotating for 16 hr at 4°C in the presence of 2.5 μl of 0.3 μg/μl anti-Cav2.3 (CACNA1E) antibody (ACC-006, Alomone Labs, Jerusalem, Israel), washed, and eluted as described above with subsequent immunoblotting analysis performed as described above.

    Techniques: Mouse Assay